Biological Trace Element Research最新文献

{"title":"Biogenic Zinc Oxide Nanoparticles for Skin Disorders: Mechanistic Insights, Translational Challenges, and Future Prospects.","authors":"Sumana Das, Susmita Kumari Yadav, Anima Pandey","doi":"10.1007/s12011-026-05200-8","DOIUrl":"10.1007/s12011-026-05200-8","url":null,"abstract":"<p><p>The rising global burden of skin disorders necessitates the development of safe, effective, and sustainable therapeutic strategies. In this review, biogenic zinc oxide nanoparticles have emerged as a next-generation platform in dermatological nanomedicine, owing to their intrinsic antimicrobial, anti-inflammatory, photoprotective, and antioxidant properties. This review highlights recent advancements in biogenic ZnONPs synthesised via plant-mediated approaches, with comparative insights into their physicochemical characteristics, safety and biological activities. Mechanistically, biogenic ZnONPs operate through multimodal pathways, including microbial membrane disruption, modulation of inflammatory signalling, modulation of reactive oxygen species, promotion of reepithelization, and protection against UV-induced oxidative damage. Also, integrate multi-omics approaches to further advance understanding of nanoparticle-cell interactions at the cellular level. However, challenges such as a lack of standardised synthesis protocol, ion-mediated cytotoxicity, oxidative stress, and limited clinical validation remain significant barriers. Overall, biogenic ZnONPs represent a sustainable and versatile platform for transdermal therapy, but their clinical translation requires standerized synthesis, comprehensive safety evaluation, and well-designed preclinical and clinical studies.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7454-7473"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spermidine Alleviates Liver Damage Induced by Iron Overload by Inhibiting Oxidative Stress.","authors":"Shuo Li, Yilian Wang, Shiyu Chen, Yuxuan Qi, Yuxin Qi, Xinyi Wang, Bo Kang","doi":"10.1007/s12011-026-05234-y","DOIUrl":"10.1007/s12011-026-05234-y","url":null,"abstract":"<p><p>Liver diseases are usually accompanied by the occurrence of oxidative stress, and imbalance in hepatic iron homeostasis is a key factor leading to hepatic oxidative stress. Spermidine, as a natural antioxidant, whether it can alleviate the oxidative stress caused by iron homeostasis imbalance remains unclear. This study examined whether spermidine could mitigate iron overload in the liver induced by dextran iron. The results indicate that liver damage caused by iron overload includes disruption of the lobular structure, proliferation of Kupffer cells, and substantial iron deposition (P < 0.001), concurrent with increased MDA and ROS levels (P < 0.001). Furthermore, iron overload markedly upregulated the expression of pro-inflammatory, including TNF-α, IL-1β, IL-6, IFN-γ, CXCL10, and IL-12 (P < 0.001). Meanwhile, iron overload elevated serum TBIL (P < 0.001) but reduced ALT and AST levels (P < 0.05). Spermidine markedly reduces hepatic MDA, ROS, and iron ion levels induced by dextran iron while simultaneously enhancing the hepatic antioxidant capacity. Mechanistically, it suppresses the expression of pro-inflammatory genes such as TNF-α, IL-1β, IL-6, CXCL-10 and IL-12 (P < 0.05), and upregulates key antioxidant proteins, including Nrf2, HO-1, and GPX4, thus alleviating liver injury induced by iron overload. Spermidine is not a direct iron chelator and does not reduce serum iron levels; instead, it indirectly regulates hepatic iron distribution and deposition via oxidative stress suppression. In conclusion, spermidine can alleviate liver toxicity caused by iron overload by reducing oxidative damage to the liver.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7928-7939"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148454343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal Evidence of Metal and Metalloid Exposure in a Free-Ranging Juvenile Southern Elephant Seal (Mirounga leonina) From the Brazilian Coast.","authors":"Leila Soledade Lemos, Salvatore Siciliano, Rachel Ann Hauser-Davis","doi":"10.1007/s12011-026-05216-0","DOIUrl":"10.1007/s12011-026-05216-0","url":null,"abstract":"<p><p>Southern elephant seals are highly mobile marine predators that can reflect environmental exposure to chemical elements through their diet and foraging ecology. However, information on elemental concentrations in elephant seal feces remains scarce, particularly outside Antarctic regions. In this study, the concentrations of several metals and metalloids, including rarely studied technology-critical elements, were determined in fecal samples from a juvenile male southern elephant seal (Mirounga leonina) from along the southwestern Atlantic coast of Brazil. Elemental analyses revealed the predominance of biologically essential elements such as Ca and Fe, whereas toxic elements generally occurred at lower concentrations. Rare earth elements, as well as Li, Rb, and Ti were also detected, consistent with recent dietary exposure. Comparisons with previous Antarctic studies indicated overall similar or lower concentrations for most elements, although higher concentrations of As, Be, Cs, Cu, Rb, and U were observed herein. These differences may be associated with regional environmental conditions as well as ontogenetic variations in diet between juvenile and adult individuals. This study provides the first baseline data on elemental concentrations in southern elephant seal feces from the southwestern Atlantic and highlights the usefulness of feces as a non-invasive matrix for monitoring recent environmental exposure in marine ecosystems.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"7702-7710"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Does Boric Acid Inhibit Cell Proliferation on MCF-7 and MDA-MB-231 Cells in Monolayer and Spheroid Cultures by Using Apoptosis Pathways?","authors":"Dilek Bayram, Meltem Özgöçmen, Dilek Aşcı Çelik, Emine Sarman, Murat Sevimli","doi":"10.1007/s12011-026-05354-5","DOIUrl":"https://doi.org/10.1007/s12011-026-05354-5","url":null,"abstract":"","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Arsenic Trioxide in MRL/lpr Mice: Disease-Context Support from Human Transcriptomic and DNA Methylation Datasets.","authors":"Yi Zhang, XiaoYu Chen, ChenXi Shi, JiaYing Fu, XinYi Chen, Feng Shao, ZhiJun Xie, XiaoBing Yang","doi":"10.1007/s12011-026-05260-w","DOIUrl":"https://doi.org/10.1007/s12011-026-05260-w","url":null,"abstract":"<p><p>To evaluate the therapeutic effects of arsenic trioxide (ATO) in lupus-prone mice and to determine whether SOCS1/JAK2/STAT3/IL-17A-related inflammatory regulation is relevant to human systemic lupus erythematosus (SLE) using public transcriptomic and DNA methylation datasets. Two in vivo experiments were performed using MRL/lpr mice. The first assessed the effects of ATO on hepatorenal injury and inflammatory gene expression. The second further evaluated these findings using C57BL/6 controls and MRL/lpr mice treated with ATO, 5-Azacytidine (5-Aza), or their combination. Serum biomarkers, histopathology, and the mRNA expression of the SOCS1/JAK2/STAT3 axis were analyzed. Network pharmacology was used to predict potential ATO-related targets and pathways in SLE. WGCNA was performed using the GSE4588 human CD4⁺ T-cell transcriptomic dataset to identify SLE-associated co-expression modules. Disease-context support was assessed using the GSE65391 whole-blood transcriptomic dataset. SOCS1 promoter-associated methylation was further evaluated using the GSE82218 PBMC DNA methylation dataset. ATO improved lupus-like manifestations in MRL/lpr mice, reduced anti-dsDNA antibodies, IgG, urinary protein, and hepatorenal injury markers, restored complement C3, and alleviated renal and hepatic inflammation. At the molecular level, ATO treatment was associated with reduced DNMT1 expression, increased SOCS1 expression, and decreased JAK2, STAT3, and IL-17A transcription. Network pharmacology highlighted Th17 cell differentiation as a candidate ATO-related pathway in SLE. WGCNA of the GSE4588 human CD4⁺ T-cell transcriptomic dataset identified SLE-associated co-expression modules and hub genes. STRING/Cytoscape analysis further identified hub genes including CD44, TLR4, THBS1, and TET2, suggesting disease-associated immune and inflammatory network remodeling in SLE CD4⁺ T cells. In GSE65391, JAK2 and STAT3 expression and JAK/STAT-related signatures were increased in SLE. In the GSE82218 DNA methylation cohort, SOCS1 promoter-associated CpG sites showed site-specific hypermethylation in SLE. ATO ameliorates lupus-like immune and hepatorenal injury in MRL/lpr mice, accompanied by DNMT1/SOCS1-related transcriptional changes and reduced JAK2/STAT3/IL-17A-related inflammatory transcription. Public human transcriptomic and DNA methylation data support the disease relevance of this regulatory context.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Basis of Ferroptosis and Pyroptosis in Lead-Induced Neurotoxicity: A Narrative Review of Current Evidence and Therapeutic Implications.","authors":"S Suvashini, P Harshitha, Aluru Parithathvi, Herman Sunil Dsouza","doi":"10.1007/s12011-026-05320-1","DOIUrl":"https://doi.org/10.1007/s12011-026-05320-1","url":null,"abstract":"<p><p>Lead (Pb) is a well-established potent neurotoxicant. Biochemically, overexposure to Pb is associated with alterations in synaptic signaling and neurotransmission, causing disruptions in neuronal activity and hence resulting in the manifestation of developmental-related disorders. Furthermore, lead-induced neurotoxicity triggers ferroptosis and pyroptosis through mechanical pathways, including oxidative stress, mitochondrial dysfunction, and neuroinflammatory signaling. Recently, the inhibition of ferroptosis and pyroptosis has been explored as a potential treatment strategy against neurodegenerative diseases. However, a comprehensive synthesis of the molecular mechanisms linking these regulated cell death pathways to Pb-induced neurotoxicity is lacking. This narrative review synthesizes the current evidence on how Pb may influence key regulators of ferroptosis and pyroptosis, including the dysregulation of iron homeostasis, inhibition of the antioxidant system, excess reactive oxygen species generation (ROS), lipid peroxidation, and NLRP3/caspase-1-mediated cleavage and gasdermin D-dependent release of proinflammatory cytokines. In addition, this review integrates the current understanding of the potential crosstalk between ferroptosis and pyroptosis, identifies critical knowledge gaps and discusses emerging therapeutic interventions targeting these regulated cell death pathways. Although the literature provides experimental links between exposure to Pb and non-apoptotic -regulated cell death, including ferroptosis and pyroptosis, across in vivo and in vitro models, studies underlying its molecular mechanisms are lacking. Hence, in-depth investigations and evaluations are necessary to provide a framework for exploring the convergence of these pathways in lead neurotoxicity, as well as for the development of therapeutic strategies against Pb-induced ferroptosis and pyroptosis in neurodegeneration.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boric Acid Induces Oxidative Stress-Associated Cytotoxicity and Modulates Wnt Signaling in MALME-3M Cells.","authors":"Şeyma Nur Çelik, Hatice İlayhan Karahan, Mustafa Soyöz, Tülay Kılıçaslan Ayna, İbrahim Pi̇ri̇m, Melek Pehli̇van","doi":"10.1007/s12011-026-05348-3","DOIUrl":"https://doi.org/10.1007/s12011-026-05348-3","url":null,"abstract":"<p><p>Melanoma is an aggressive malignancy with high metastatic potential and frequent treatment resistance. Boric acid (BA), a bioactive boron-derived compound, has shown anticancer activity in several tumor models, although its molecular effects in melanoma remain incompletely understood. This study investigated the antiproliferative effects of BA in MALME-3M metastatic melanoma cells, with emphasis on oxidative status, apoptosis-related markers, cell-cycle progression, migration-related features, and Wnt signaling-associated molecules. Cell viability and clonogenic potential were assessed using MTT and colony-formation assays. Apoptosis-related changes were evaluated by RT-qPCR and Annexin A5 (ANXA5) ELISA. Cell-cycle distribution was evaluated descriptively by flow cytometry. Wnt pathway-related molecules were examined by RT-qPCR and Western blotting, while oxidative status was evaluated using Total Oxidant Status (TOS), Total Antioxidant Status (TAS), and Oxidative Stress Index (OSI) measurements. BA reduced cell viability in a concentration- and time-dependent manner and markedly reduced clonogenic capacity. BA treatment was associated with increased TOS, decreased TAS, altered apoptosis-related gene expression, increased ANXA5 levels, and G0/G1 accumulation with reduced S-phase representation. BA treatment also showed a tendency toward reduced wound closure and altered MMP2 and TIMP1 expression. The molecular expression profile was consistent with reduced canonical Wnt/β-catenin signaling and modulation of non-canonical Wnt-related components. Overall, BA exerted antiproliferative effects in MALME-3M cells accompanied by oxidative stress-associated cytotoxicity and changes in apoptosis-, cell-cycle-, migration-, and Wnt signaling-related markers. These findings support further validation of BA in additional melanoma models.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149029649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report on Metal Contamination and Associated Human Health Risks in the Smooth Butterfly Ray Gymnura cf. micrura (Myliobatiformes: Gymnuridae) from the Brazilian Amazon Coast.","authors":"Iris Valquiria Medeiros da Cruz Teixeira, Tatiana Dilllenburg Saint'Pierre, Getulio Rincon Filho, Rachel Ann Hauser-Davis","doi":"10.1007/s12011-026-05337-6","DOIUrl":"https://doi.org/10.1007/s12011-026-05337-6","url":null,"abstract":"<p><p>Metal accumulation in batoids reflects both environmental exposure and physiological regulation, although studies concerning this group are still scarce. The Smooth butterfly ray (Gymnura cf. micrura) is consumed in northeastern Brazil, yet information on metal contamination and associated health risks are unavailable to date. This study assessed metal and metalloid concentrations, muscle metallothionein (MT) levels, elemental associations, and human health risks in specimens collected off the Maranhão coast. No significant sex-related differences were observed in element concentrations or MT levels, and MT showed no significant correlations with metals, suggesting limited muscle involvement in detoxification or exposure levels below induction thresholds. Multivariate and correlation analyses indicated distinct accumulation patterns, with rare earth elements associated with lithogenic inputs and some essential-toxic element relationships suggesting possible antagonistic interactions, particularly involving Se and Hg. A risk assessment showed that estimated daily and weekly intake values remained below guidance limits under realistic inorganic As assumptions. However, non-carcinogenic and carcinogenic risks increased under conservative scenarios, especially for infants at higher consumption frequencies. Although a positive HBVSe value suggests possible Se-mediated protection against Hg toxicity, this effect does not eliminate overall contaminant-related health risks.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Optimization of Selenium Inclusion Level in the Larval Diet of Labeo rohita.","authors":"Shanza Gul, Amina Zuberi, Javeria Shamas, Mashooq Ali, Muhammad Kamran","doi":"10.1007/s12011-026-05352-7","DOIUrl":"https://doi.org/10.1007/s12011-026-05352-7","url":null,"abstract":"","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Determination of Lead, Cadmium, Mercury, and Arsenic in Commercial Dairy Products by Atomic Absorption Spectrometry and Inductively Coupled Plasma Optical Emission Spectrometry.","authors":"Mohamed M A Zeinhom, Hassan A Rudayni, Haifa E Alfassam, Waleed R El-Ghareeb, Ayman E Taha, Ahmed A Allam","doi":"10.1007/s12011-026-05345-6","DOIUrl":"https://doi.org/10.1007/s12011-026-05345-6","url":null,"abstract":"<p><p>Heavy metal contamination of dairy products represents a significant food safety concern because of the potential for chronic dietary exposure and associated health risks. This study determined the concentrations of lead (Pb), cadmium (Cd), mercury (Hg), and arsenic (As) in commercial milk, white soft cheese, and yoghurt marketed in Egypt using atomic absorption spectrometry (AAS) and inductively coupled plasma optical emission spectrometry (ICP-OES), followed by dietary exposure and pollution index assessments. A total of 150 samples (50 of each dairy product) were collected from retail markets and analyzed following acid digestion. AAS detected Pb concentrations ranging from 0.021 to 0.194 mg/kg, Cd from 0.010 to 0.186 mg/kg, Hg from 0.033 to 0.060 mg/kg, and As from 0.122 to 0.154 mg/kg. Corresponding ICP-OES concentrations ranged from 0.013 to 0.182 mg/kg for Pb, 0.0092 to 0.114 mg/kg for Cd, 0.012 to 0.038 mg/kg for Hg, and 0.097 to 0.132 mg/kg for As. The two analytical techniques showed consistent patterns of variation among dairy matrices, although systematic method-dependent differences were observed, with ICP-OES generally yielding lower concentrations than AAS with relative differences ranging from 6.19% to 76.00%, depending on the metal and dairy matrix. Estimated daily and weekly intakes of all investigated metals were below internationally recognized health-based guidance values for adult consumers. However, pollution index analysis revealed that Pb and Cd concentrations exceeded the maximum permissible limits in all dairy products, while Hg exceeded the regulatory limit only in yoghurt, remained at the permissible limit in milk, and complied with the regulatory limit in white soft cheese. No regulatory maximum permissible limit has been established for As in these dairy products. These findings demonstrate the usefulness of both AAS and ICP-OES for trace-element determination while emphasizing the importance of rigorous method validation and continuous monitoring to ensure the safety of dairy products and protect public health.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}