Biological Trace Element Research最新文献

{"title":"Assessment of Elemental Deficiency of Crossbred Dairy Cows and Mineral Composition in Natural Feed and Nutritional Supplements in the Northern and Northwestern Provinces in Sri Lanka.","authors":"Joseph W Clarkson, Neil I Ward, Joaquín M Prada, David Tisdall, Mónica Felipe-Sotelo, J K Vidanarachchi, Mike Christian, Mark Andrew Chambers","doi":"10.1007/s12011-024-04299-x","DOIUrl":"10.1007/s12011-024-04299-x","url":null,"abstract":"<p><p>This study assessed the elemental status of cross-bred dairy cows in small holder farms in Sri Lanka, with the aim to establish the elemental baseline and identify possible deficiencies. For this purpose, 458 milk, hair, serum and whole blood samples were collected from 120 cows in four regions of Northern and Northwestern Sri Lanka, (namely Vavaniya, Mannar, Jaffna and Kurunegala). Farmers also provided a total of 257 samples of feed, which included local fodder as well as 79 supplement materials. The concentrations of As, Ca, Cd, Co, Cr, Cu, Fe, I, K, Mg, Mn, Mo, Na, Ni, Pb, Se, V and Zn were determined by inductively coupled plasma mass spectrometry (ICP-MS). Evaluation of the data revealed that all cows in this study could be considered deficient in I and Co (18.6-78.5 µg L^-1 I and 0.06-0.65 µg L^-1 Co, in blood serum) when compared with deficiency upper boundary levels of 0.70 µg L^-1 Co and 50 µg L^-1 I. Poor correlations were found between the composition of milk or blood with hair, which suggests that hair is not a good indicator of mineral status. Most local fodders meet dietary requirements, with Sarana grass offering the greatest nutritional profile. Principal component analysis (PCA) was used to assess differences in the elemental composition of the diverse types of feed, as well as regional variability, revealing clear differences between forage, concentrates and nutritional supplements, with the latter showing higher concentrations of non-essential or even toxic elements, such as Cd and Pb.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"2029-2040"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin Treatment Ameliorates Hepatic Insulin Resistance Induced by Sub-chronic Oral Exposure to Cadmium LOAEL Dose via NF-κB and Nrf2 Pathways.","authors":"Victor Enrique Sarmiento-Ortega, Diana Moroni-González, Alfonso Diaz, Eduardo Brambila, Samuel Treviño","doi":"10.1007/s12011-024-04314-1","DOIUrl":"10.1007/s12011-024-04314-1","url":null,"abstract":"<p><p>Cadmium (Cd) is a global pollutant, and its accumulation in the liver causes oxidative stress, inflammation, insulin resistance (IR), and metabolic complications. This study investigated whether curcumin treatment could alleviate hepatic IR in Wistar rats exposed to sub-chronic cadmium and explored the underlying molecular pathways. Male Wistar rats were divided into a control group (standard normocaloric diet + cadmium-free water) and a cadmium group (standard normocaloric diet + drinking water with 32.5 ppm CdCl₂) for 30 days. Oral glucose tolerance, insulin response, and IR were assessed using mathematical models. Liver tissue was analyzed for markers of oxidative stress, inflammation, and key regulatory pathways, including NF-κB, Nrf2, MAPKs (JNK and p38), and the IRS1-Akt pathway. We established an effective curcumin dose of 250 mg/kg for 5 days orally. Results demonstrated that after 30 days of exposure, cadmium accumulated in the liver, inducing an oxidative and inflammatory state. This was characterized by increased expression of NF-κB, JNK, and p38, along with diminished Nrf2 expression, hepatic IR, hyperglycemia, and hyperinsulinemia. Curcumin treatment effectively alleviated these metabolic disorders by restoring the balance between NF-κB and Nrf2 in the liver, modulating the MAPK pathway, and, consequently, improving oxidative and inflammatory balance. In conclusion, this study suggests that cadmium induces hepatic IR through an imbalance between NF-κB and Nrf2 signaling pathways. Curcumin treatment appears to improve these pathways, thereby ameliorating hepatic IR.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":"2382-2393"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141892796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metallic Makos: Metal and Metalloid Levels and Human Health Risks Arising from the Consumption of Shortfin Makos (Isurus oxyrinchus) from Southeastern Brazil.","authors":"Júlia Vianna de Pinho, Isabel Quental Willmer, Amanda Pontes Lopes, Regina Fonsêca, Tatiana Dillenburg Saint'Pierre, Patricia Charvet, Acácio Ribeiro Gomes Tomas, Rachel Ann Hauser-Davis","doi":"10.1007/s12011-025-04572-7","DOIUrl":"https://doi.org/10.1007/s12011-025-04572-7","url":null,"abstract":"<p><p>Sharks are among the most threatened vertebrate groups, facing pressures from overfishing, habitat loss, and pollution, with the latter posing risks not only to marine ecosystems but also to human health due to the global consumption of shark meat. This study assessed the concentrations of essential, potentially toxic, and toxic metals in the muscle tissue of 61 shortfin makos (Isurus oxyrinchus), an endothermic pelagic species, caught by industrial fleets off the coast of São Paulo, Southeastern Brazil. Several elements, including Co, Ni, V, Rb, and Ti, are reported herein for the first time in this species, expanding the understanding of metal bioaccumulation in pelagic sharks. High levels of toxic elements such as As, Cd, Cr, Hg, and Pb, along with potentially toxic elements like Ti, were detected, with concentrations of As and Hg exceeding food-safety thresholds, raising significant public health concerns. Shortfin makos may present partial physiological resilience to environmental stressors due to endothermy; although, the elevated concentrations of neurotoxic metals like Hg and significant levels of Cu and Ti detected herein highlight its vulnerability to contamination. Moderate positive correlations between metallothionein (MT) and Ti and Rb, and a moderate negative correlation with Co, suggest selective detoxification pathways; although, the limited correlation with other elements points to restricted MT-mediated protective mechanisms in muscle tissue. The relatively high MT levels observed may reflect an adaptive response to chronic exposure rather than effective detoxification. An elevated hazard index indicates that even when individual contaminants are within acceptable limits, their combined effects pose considerable health risks. Given species-specific variability in metal accumulation and the absence of toxicological thresholds for elasmobranchs, future research should aim to define these limits to guide both conservation strategies and public health policies. Ecotoxicological assessments can serve as indirect conservation tools by highlighting human health risks associated with the consumption of threatened species, potentially discouraging their exploitation.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Combined Effect Between SEPS1 Genetic Polymorphisms and Exposure to Metals on the Risk of Gestational Diabetes Mellitus.","authors":"Weiwei Wu, Yulin Li, Weixuan Hu, Jing Zhao, Bole Zhang, Yongliang Feng, Yawei Zhang, Suping Wang","doi":"10.1007/s12011-025-04597-y","DOIUrl":"https://doi.org/10.1007/s12011-025-04597-y","url":null,"abstract":"<p><p>Environmental metal exposure is a key risk factor for gestational diabetes mellitus (GDM), but the mechanisms remain unclear. The traditional view holds that excessive accumulation of metals directly damages the islets to induce GDM. Recent studies suggest that supplementing with trace elements can alleviate insulin resistance by reducing endoplasmic reticulum stress, indicating that endoplasmic reticulum stress might have a comparable impact in metal-induced GDM. Selenoprotein S (SEPS1), a key regulator of endoplasmic reticulum function, may play a role. This study aims to investigate the interaction between the genetic predisposition of the SEPS1 gene and exposure to metals on GDM from the perspective of endoplasmic reticulum stress. A total of 278 pregnant women with GDM and 278 matched pregnant women without GDM were recruited. Maternal blood samples were collected before delivery to genotype the SEPS1 gene and measure metal levels. We found that rs28533324 and rs894317 were associated with the risk of GDM. The level of chromium (Cr) Q3 in maternal blood increased the risk of GDM, and the level of nickel (Ni) Q4 decreased the risk of GDM. Furthermore, the Q3 and Q4 Cr exhibit a multiplicative interaction with rs894317 in both the codominant and dominant models, and a multiplicative interaction between the Cr Q3 and rs894317 is also observed in the allele model. We found a novel link between SEPS1 gene variation and GDM, with these associations potentially being modified by Cr exposure. Our findings provide new etiological insights into GDM induced by Cr through endoplasmic reticulum stress.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Higher Intakes of Dietary Zinc with Higher Ferritin or Hemoglobin: a Cross-sectional Study from NHANES (2017-2020).","authors":"Hanieh Abdi, Mohammad Safargar, Mihnea-Alexandru Găman, Camellia Akhgarjand, Kousalya Prabahar, Haniye Zarezadeh, Xin Ying Chan, Parsa Jamilian, Hamed Kord-Varkaneh","doi":"10.1007/s12011-025-04589-y","DOIUrl":"https://doi.org/10.1007/s12011-025-04589-y","url":null,"abstract":"<p><p>Zinc is an essential trace element that plays a critical role in numerous physiological processes, including immune function, cell growth, and particularly iron metabolism. This study aimed to explore the association between dietary zinc intake and key biomarkers of iron status, specifically serum ferritin and hemoglobin levels, in a representative sample of the US population. A cross-sectional analysis was conducted using data from the 2017-2020 National Health and Nutrition Examination Survey (NHANES), which included 15,560 participants. Dietary zinc intake was assessed through 24-h dietary recall, and serum ferritin and hemoglobin levels were measured through laboratory tests. Participants were grouped into quartiles based on their zinc intake. Multivariable linear regression models were applied to evaluate the relationship between dietary zinc intake and ferritin and hemoglobin levels, adjusting for potential confounders such as age, sex, body mass index (BMI), waist circumference, energy intake, macronutrients, and micronutrients. These adjustments ensured a more accurate analysis of the associations between zinc intake and iron markers. Our findings demonstrate that higher dietary zinc intake is significantly associated with elevated serum ferritin (B = 6.17, p = 0.027) and hemoglobin (B = 0.048, p = 0.019) levels, independent of other factors. Participants in the highest zinc intake quartile also showed higher hematocrit and mean corpuscular volume (MCV) levels compared to those in the lowest quartile. These results suggest that adequate dietary zinc intake may play a crucial role in improving iron status and reducing the risk of iron-deficiency anemia, particularly in populations at risk. Future research is necessary to further elucidate the mechanisms behind this relationship and to inform public health nutritional guidelines.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Components in the Breast Milk of Passive Smoker Women: A Systematic Review and Health Risks Assessment.","authors":"Nazila Moftian, Hossein Arfaeinia, Farshid Soleimani, Sara Dadipoor, Somayyeh Dehghani, Nayereh Rezaie Rahimi, Maryam Aghel, Mohammad Mahdi Hatami","doi":"10.1007/s12011-025-04595-0","DOIUrl":"https://doi.org/10.1007/s12011-025-04595-0","url":null,"abstract":"<p><p>Breast milk from passive smokers may contain various harmful chemical components, primarily due to exposure to tobacco smoke. This systematic review was conducted on the chemical components of breast milk in women who were exposed to environmental tobacco smoke (ETS). English language-published papers were advanced searched without time limitation until April, 2024 in Web of Science, PubMed, and Scopus databases. A total of 1156 papers were recorded in the initial search of these databases. Finally, 18 articles met the inclusion criteria and were included. Our results showed that metals (n = 10) and heterocyclic compounds (n = 9) (cotinine, nicotine, and nornicotine) were the most commonly reported compounds in the included studies. Cotinine was the most frequently reported compound in mature milk (n = 9) and colostrum (n = 3). Among metals in mature milk, cadmium (n = 8) and lead (n = 6) are the most common compounds. Metals in colostrum were just reported in one study. The cancer risk (CR) values showed a decreasing pattern in the order of Sr > Mn > U > Co > Pb > Cr > As > Cd > Be for both aged infant groups. Parents should be encouraged to not smoke, as well as must be advised of the potential risks of ETS exposure to their babies. It is recommended that routine and continuous monitoring of probable sources of chemical contamination of breast milk due to environmental exposures should be done to discover the influencing factors and develop suitable interventions for decreasing the exposure/contamination.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Heavy Metals and Essential Minerals in the Hair of Children with Autism Spectrum Disorder and Their Association with Symptom Severity.","authors":"Xulan Zhou, Xiaochun Xia, Liming Li, Yaohui Ye, Qihui Chen, Mingyue Ke, Qian Cui, Yuling He, Yiting Chen, Shaoqing Lin, Wenlong Liu, Juan Wang","doi":"10.1007/s12011-025-04588-z","DOIUrl":"https://doi.org/10.1007/s12011-025-04588-z","url":null,"abstract":"<p><p>The exact cause of Autism spectrum disorder (ASD) remains unclear. The accumulation of heavy metals and the imbalance of trace elements are believed to play a key role in the pathogenesis of ASD. This study aimed to compare the levels of trace elements and heavy metals in the hair of 1-16-year-old children with varying ASD severity. We included a control group of 57 children, as well as 124 children with autism, consisting of 53 with mild to moderate autism and 71 with severe autism. Questionnaires and hair samples were collected, and 21 chemical elements were analyzed using inductively coupled plasma mass spectrometry (ICP-MS). Children with severe autism showed significantly higher levels of the trace elements copper (Cu) and heavy metals vanadium (V), cobalt (Co), nickel (Ni), arsenic (As), cadmium (Cd), and lead (Pb) in their bodies compared to the control group. Boys with severe autism showed significantly higher levels of Cu, As, Cd, and Pb compared to the control group, while girls with severe autism exhibited significantly lower levels of potassium (K) and increased levels of Pb. Severely autistic children under 7 years old had significantly increased levels of Mn, Cu, V, Co, Ni, As, Cd, and Pb. Children with severe autism aged 7-16 years typically showed significantly higher levels of Cu and As. These findings underscore the importance of heavy metals and essential minerals as environmental factors in the severity of ASD disease.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Different Doses and Routes of Acute Cadmium Exposure With Bone Marrow Hypoplasia, Cellular and Biochemical Alterations in Male Wistar Rats.","authors":"Damir Suljević, Vojo Milanović, Maja Mitrašinović-Brulić","doi":"10.1007/s12011-025-04591-4","DOIUrl":"https://doi.org/10.1007/s12011-025-04591-4","url":null,"abstract":"<p><p>Cadmium is a widespread and non-biodegradable pollutant that is dangerous to living organisms. The routes of exposure and doses of pollutants are different, and it is extremely important to assess their toxicity. Three experimental groups received a single treatment of CdCl₂ (15 and 30 mg/kg orally; 15 OR and 30 OR group and 15 mg/kg intraperitoneally; 15 IP group) and one control group (Ctr). The toxic effects of Cd were examined on hematological and biochemical parameters, and histopathological observation of hepatocytes and bone marrow. Leukocytopenia and granulopenia were recorded in 30-OR, and thrombocytopenia in 15-OR and 15-IP. 30-OR causes a decrease in RBC, and 15-IP causes changes in RBC count. Renal markers (CRE and BUN) show a correlation with 15-OR dose, inflammatory marker CRP shows a positive correlation with 15-IP dose, LDH as a biomarker of oxidative stress and CK as a biomarker of membrane damage were significantly increased in all experimental groups. The hepatocyte membrane and the size of the nucleolus have changed in all groups, and the highest degree in 15-IP. Bone marrow hypoplasia was noted with oral doses, and basophilia and an increased number of lymphoblasts and myeloblasts and immature hematopoietic cells with toxic granulations in 15-IP. Single doses of Cd cause serious toxicological changes in blood and tissues. Oral doses cause significant tissue-specific microscopic lesions observed in the liver during histopathology and bone marrow hypoplasia compared with intraperitoneal administration. Intraperitoneal administration shows a strong correlation with renal, inflammatory and stress markers compared to oral administration.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationships Between Soil and Egg Parameters and Lead and Cadmium Concentrations in Eggshell and Egg Content of Collared Pratincole (Glareola pratincola).","authors":"Shahryar Karamati, Seyed Mehdi Amininasab, Mohammadreza Tatian, Fardin Sadeghzadeh","doi":"10.1007/s12011-025-04592-3","DOIUrl":"https://doi.org/10.1007/s12011-025-04592-3","url":null,"abstract":"<p><p>Understanding the relationship between breeding habitats and pollutants is crucial for the reproductive success of birds. This study aimed to determine the relationships between egg characteristics and the concentrations of lead (Pb) and cadmium (Cd) in the habitat as well as in the eggshells and egg contents of the Collared Pratincole (Glareola pratincola). The study was conducted in northern Iran in the spring of 2022. Thirty nests were randomly selected and monitored until the clutch size was completed. One egg and soil samples were collected from each nest. Soil characteristics included the concentrations of Pb and Cd. Egg characteristics measured included laying order, weight, shell thickness, egg shape index, clutch size, and the concentrations of Pb and Cd in both the eggshell and egg content. The results showed that the concentrations (mg/kg) of Pb (90.47 ± 6.86) and Cd (10.29 ± 0.67) in the eggshell were significantly higher than those in the egg content (Pb: 8.72 ± 5.05; Cd: 0.64 ± 0.38) (P ≤ 0.001). A higher accumulation of these trace elements was observed in eggshells from eggs containing older embryos (Pb: P = 0.003, Cd: P = 0.022). A significant correlation was also found between Pb concentrations in the eggshell and the egg content (P = 0.016) as well as between Pb and Cd levels in the eggshell (P ≤ 0.001). This study indicates that measuring trace elements in both the eggshell and egg content can be a useful method for monitoring the potential transfer of environmental pollutants from mother to embryo. The results provide baseline information regarding the concentration of trace elements in the eggs of the Collared Pratincole and the potential for transfer from the mother to embryo, which may be used for ecotoxicology, physiology, and genetic studies to inform conservation and management plans.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targets and Potential Mechanism of Chondroitin Sulfate A-selenium Nanoparticle on Kashin-Beck Disease Chondrocytes.","authors":"Huan Deng, Feidan Deng, Xinyue Wen, Yude Jiang, Abebe Feyissa Amhare, Lichun Qiao, Miaoqian Li, Ping Wan, Jun Wang, Jing Han","doi":"10.1007/s12011-025-04584-3","DOIUrl":"https://doi.org/10.1007/s12011-025-04584-3","url":null,"abstract":"<p><p>Kashin-Beck disease (KBD) is a chronic and deformable osteoarthropathy linked to low selenium. Limited cartilage regeneration poses challenges for its treatment. Previous studies have found that chondroitin sulfate A-selenium nanoparticle (CSA-SeNP) protects chondrocytes. This study used label-free LC-MS/MS quantitative proteomics to identify differentially expressed proteins and pathways in KBD chondrocytes post-CSA-SeNP treatment. Western blot (WB) was used to verify the key differential proteins, and transmission electron microscopy (TEM) was used to observe the ultrastructure of chondrocytes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed proteins mainly involved in RNA processing, translation, peptide biosynthesis, gene expression, rRNA metabolism, and ribonucleoprotein complex biogenesis. Notably, 121 proteins were up-regulated, 27 down-regulated, and 117 specifically expressed in the CSA-SeNP group. Enriched KEGG pathways included ribosome biogenesis, mRNA surveillance, endoplasmic reticulum protein processing, and endocytosis. Proteins related to autophagy, ER stress, cell homeostasis, protein processing and transport, including PELO, WES1, PLAA, RRBP1, ARC1B, ARFGAP2, and SH3KBP1, were significantly up-regulated in the CSA-SeNP group. In conclusion, our results demonstrated that CSA-SeNP may exert protective effects on chondrocytes in adult patients with KBD mainly through the regulation of target proteins and pathways related to ribosome biogenesis, mRNA surveillance, endoplasmic reticulum protein processing, endocytosis, autophagy, ER stress, and cell homeostasis. Further studies with larger sample size and in vivo to identify, screen and verify the regulatory effects of target proteins and pathways may provide more information for elucidating the mechanism of CSA-SeNP in the treatment of KBD.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}