Biological Trace Element Research最新文献

{"title":"Assessment of Elements in Black Tea Infusions by Brewing Method in Terms of their Nutritional Value.","authors":"Maria Długaszek, Jadwiga Mierczyk, Wojciech Skrzeczanowski, Maria Długaszek","doi":"10.1007/s12011-025-04629-7","DOIUrl":"https://doi.org/10.1007/s12011-025-04629-7","url":null,"abstract":"<p><p>Tea infusions contain many valuable bioactive compounds and elements. The research is aimed at examining them in terms of nutritional value and determining the optimal conditions for their preparation. The study determined the content of Ca, Cu, Fe, K, Mg, Mn, Na, Zn, and Al in infusions of black tea, including bagged and loose leaf. An atomic absorption spectrometer was used for quantitative instrumental analysis. The obtained results were subjected to statistical analysis including PCA (Principal Components Analysis). The brewing time was 5, 10 and 20 min, and the pH value of the infusions was 2 and 6. It was found that, generally, both parameters increased the content of elements in infusions. The amount of elements extracted from 1 g of tea was within the range: Ca- ND (not detected)-7.23 mg, K- 15.1-32.3 mg, Mg- 0.11-0.86 mg, Na- ND-2.85 mg, and Al- ND- 1028 µg, Cu- 1.24-11.02 µg, Fe- ND-36.13 µg, Mn- 97.3-541.6, and Zn- 6.18-22.43 µg. These data were compared with the results for green tea infusions presented in a previous study. The results of this study suggest that the amount of Al and Mn in tea infusions should be controlled.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic Insights Into the Role of Selenoprotein M in Nickel-Induced Lung Fibrosis.","authors":"Haoyue Guan, Yue Sun, Senqiu Qiao, Di Li, Jingzeng Cai, Ziwei Zhang","doi":"10.1007/s12011-025-04636-8","DOIUrl":"https://doi.org/10.1007/s12011-025-04636-8","url":null,"abstract":"<p><p>Long-term exposure to high concentrations of nickel (Ni) compounds could cause damage to lung tissue and increase the risk of lung and respiratory cancers. Selenoprotein M (SELENOM) plays a crucial role in antioxidant and anti-inflammatory activities. However, the relationship between SELENOM and the mechanism of Ni-induced pulmonary fibrosis in mice remains unknown. Our study explored the regulated mechanism of SELENOM in Ni-induced pulmonary fibrosis. Wild-type and SELENOM knockout C57BL/6N male mice were randomly divided into Wild-control and Wild-Ni groups, which were administered distilled water and NiCl₂ (10 mg/kg) by gavage for 21 days. Lung tissues were then collected for histological analysis using hematoxylin-eosin (H&E) and Masson staining, as well as for electron microscopic examination. Firstly, light microscopy revealed inflammatory cell infiltration, alveolar collapse, and alveolar wall thickening in the lung tissue of SELENOM knockout mice. Electron microscopy of lung tissue showed a large accumulation of fibroblasts, proliferation of collagen fibers, and dense collagen deposition, indicating that SELENOM knockout increased lung injury in Ni treatment. Secondly, SELENOM knockout increased malondialdehyde (MDA) levels while decreasing superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-Px) activities. Furthermore, Ni exposure and SELENOM knockout significantly upregulated protein and mRNA levels of epithelial-mesenchymal transition (EMT) markers α-SMA, COL-I, TGF-β1/Smad, and JAK2/STAT3 signaling pathway in the lung. These findings suggest that SELENOM knockout promotes EMT and exacerbates pulmonary fibrosis and inflammation through activation of the TGF-β1/Smad and JAK2/STAT3 signaling pathways. In summary, our study highlights the critical role of SELENOM in mitigating Ni-induced pulmonary fibrosis and provides insights into potential therapeutic targets for Ni-induced lung diseases.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Lycium Barbarum Polysaccharides and Sodium Selenite on Cadmium Chloride-Induced Testicular Toxicity in Rats.","authors":"Xueling Zhang, Yongxin Zhang, Ting Wang, Deng Xiao, Jianding Wang, Chao Ma, Tong Liu, Duoming Zhao, Sheng Li, Junling Wang, Chengyun Li","doi":"10.1007/s12011-025-04637-7","DOIUrl":"https://doi.org/10.1007/s12011-025-04637-7","url":null,"abstract":"<p><p>Cadmium (Cd) is a major environmental pollutant associated with male reproductive health. Lycium barbarum polysaccharides (LBP) and selenium (Se) have been shown to protect against testicular damage. Male rats (180-200 g) were selected to construct a Cd-exposed model by intraperitoneal injection with concentrations of 0.0, 0.5, 1.0, 1.5, and 2.0 mg/kg. Then, LBP and sodium selenite (Na₂SeO₃) were given by gavage for treatment. The results showed that Cd accumulates in the testis in a dose-dependent manner, and compared with the control group, after Cd-exposed, the testis suffered significant toxic damage. The differences in mRNA enrichment analysis between the Cd-exposed and the control groups showed that the differential genes were mainly involved in peroxidase activity, iron regulation, and cellular energy metabolism. Compared with the Cd-exposed group, LBP and Na₂SeO₃ intervention could antagonize the toxic damaging effects caused by Cd, and the combined intervention of LBP and Na₂SeO₃ could better antagonize the toxic damaging effects caused by Cd compared with the intervention alone. In general, Cd exposure caused testicular damage, increased testicular hormonal disorders, oxidative stress damage, energy metabolism disorders, and iron atrophy in the testis, while treatment with LBP and selenium alleviated the effects of Cd-induced testicular toxicity damage.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano Zinc Oxide Restores Gut Barrier Integrity and Modulates Microbiota to Mitigate TNBS-Induced Colitis in Mice.","authors":"Zhengqiang Yu, Yi Qiu, Yingxiang Yang, Changlin Wen, Shiyuan Huang, Tao Liu, Rao Zhong, Xi Peng","doi":"10.1007/s12011-025-04635-9","DOIUrl":"https://doi.org/10.1007/s12011-025-04635-9","url":null,"abstract":"<p><p>Inflammatory bowel diseases (IBD) are chronic, relapsing gastrointestinal disorders with complex pathological mechanisms and limited treatment options, caused by various uncertain factors. Zinc plays a crucial role in wound healing, tissue regeneration, and immune responses. While zinc deficiency is common among patients with IBD, the effects of dietary zinc supplementation on the disease course remain unclear. In this study, 125 male C57BL/6 J mice were randomly divided into five groups: a control group (Con), a 2,4,6-trinitrobenzenesulfonic acid (TNBS) induction group, and three zinc supplementation groups receiving 160 ppm, 400 ppm, and 1000 ppm of zinc oxide nanoparticles ( ZnO NPs), respectively. The results showed that dietary supplementation with ZnO NPs significantly alleviated damage to the colonic mucosal epithelial cells and crypts in IBD mice, while effectively reducing the inflammatory response. Furthermore, ZnO NPs helps maintain the quantity and secretion function of goblet cells, restores normal expression levels of tight junction proteins (ZO-1, occludin), and proliferating cell nuclear antigen (PCNA), and preserves the diversity of gut microbiota. Notably, a significant protective effect was observed with dietary zinc supplementation at 160 ppm. These findings suggest that ZnO NPs significantly improves TNBS-induced intestinal inflammatory damage by modulating the gut microbiota, mucus, and mechanical barriers.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elemental Composition of Women's Fingernails: A Comparative Analysis Between Cervical Cancer Patients and Healthy Individuals.","authors":"Hanh Van Nguyen, Sang Thi Minh Nguyen, Huong Thi Thu Tran, Loan Thi Hong Truong, Dong Van Nguyen, Linh Thi Truc Nguyen, Ba Ngoc Vu, Phuong Truc Huynh","doi":"10.1007/s12011-025-04628-8","DOIUrl":"https://doi.org/10.1007/s12011-025-04628-8","url":null,"abstract":"<p><p>This study investigates elemental composition differences in fingernails between cervical cancer patients and healthy individuals using the Total Reflection X-ray Fluorescence (TXRF) method. A total of 43 fingernail samples from women diagnosed with cervical cancer and 40 from age-matched healthy controls were analyzed. The results revealed significantly higher concentrations of essential elements (Mn, Fe, and Cu) and toxic elements (Cr, As, Br, and Pb) in cancer patients. In contrast, essential elements such as Ca, Zn, and Se were found at lower levels in cancerous patients. The deficiencies in Ca, Zn, and Se are likely influenced by dietary factors, whereas the elevated levels of Cr, Mn, Fe, Cu, As, Br, and Pb may be linked to environmental pollution. Strong positive correlations of elements in the fingernails of cancer patients were found between K-As, Ca-Se, Ti-Cr, Ti-Fe, Ti-Cu, Ti-Pb, Cr-Fe, Cr-Pb, Fe-Pb, Cu-Pb, As-Pb, and Sb-Ba. In contrast, strong negative correlations were observed between Ca-Ti, Ca-Pb, Cr-Zn, Fe-Zn, Cu-Zn, Cu-Se, Zn-Pb, and Se-Pb. Meanwhile, a strong positive correlation between Zn-Se and a strong negative correlation between K-Se were observed in the fingernails of healthy controls. Principal component analysis (PCA) showed the essential and toxic elements shift between cancer patients and healthy controls. The study showed significant differences in element distribution patterns and interrelationships in the fingernails of cancer patients compared to the controls. It was concluded that the considerable changes in fingernail element concentrations were associated with disease risk, specifically cervical cancer. Monitoring and regulating the intake of essential elements while controlling exposure to toxic elements could be beneficial for cervical cancer patients.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mercuric Chloride Aggravates Hyperglycemia-Induced Anxiety and Depressive-Like Behaviors in Type 2 Diabetic Rats: Breakdown of the Antioxidant Defense System.","authors":"Benloughmari Douae, Bikri Samir, El Aboubi Meriam, Yassif Fatima-Zahra, Aboussaleh Youssef","doi":"10.1007/s12011-025-04640-y","DOIUrl":"https://doi.org/10.1007/s12011-025-04640-y","url":null,"abstract":"<p><p>Introduction Type 2 diabetes mellitus (T2DM) is a global health problem frequently associated with biochemical disturbance and also, with a range of mental health disorders including such as anxiety and depression. Whereas, mercury chloride (HgCl₂) is a common environmental pollutant, which is neurotoxic and induces oxidative stress, especially in metabolic disorders like diabetes. The purpose of this investigation is to evaluate the interaction between hyperglycemia-induced oxidative stress and HgCl₂ toxicity and to assess their far-reaching effect spotlighted on biochemical and behavioral disturbances. By analyzing key oxidative stress markers and anxiety- and depression-like behaviors. Experimental design was carried out as follow: control group, HgCl₂-treated group, diabetic group and diabetic HgCl₂-treated group. Type 2 diabetes was induced in a diabetic model via streptozotocin (STZ) and nicotinamide (NA) injections. For the HgCl₂-exposed groups, rats were administered 0.375 mg/kg/day of HgCl₂ orally for 45 consecutive days. Additionally, behavioral tests were performed to examine anxiety- and depression-like behaviors, and hematological, biochemical, oxidative stress markers were assessed to evaluate systemic and neurotoxic effects. The results showed significant increases in fasting blood glucose levels in diabetic and HgCl₂-treated diabetic groups compared to controls (p < 0.001). Body weight significantly decreased in all treated groups (p < 0.05), with the greatest reduction observed in the HgCl₂-treated diabetic group. Behavioral analysis revealed heightened anxiety and depression-like behaviors, particularly in the HgCl₂-treated diabetic group (p < 0.05). Biochemical assessments indicated significant disruptions in lipid profiles and hepatic and renal markers, with pronounced effects in HgCl₂-treated diabetic rats (p < 0.05). Oxidative stress markers demonstrated elevated malondialdehyde and nitric oxide levels in the liver, hippocampus, and prefrontal cortex, paired with diminished antioxidant defences, including catalase and superoxide dismutase activities (p < 0.05). These findings underscore the synergistic role of hyperglycemia and HgCl₂ exposure in amplifying oxidative damage and emotional disturbances, suggesting a critical interplay between metabolic and neurotoxic pathways.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastrointestinal-Ocular Toxicity Following Systematic Ingestion of Nickel-Cadmium Contaminated Water in Wistar Rats Demonstrates Possible Health Complications Characterized by Oxidative Stress, Inflammatory and Histological Changes.","authors":"Chiedu Onyinye Emeninwa, Augustine Apiamu, Samuel Ogheneovo Asagba","doi":"10.1007/s12011-025-04622-0","DOIUrl":"https://doi.org/10.1007/s12011-025-04622-0","url":null,"abstract":"<p><p>The study evaluated the biochemical and histopathological alterations of subacute nickel (Ni) and cadmium (Cd) exposure on the gastrointestinal and ocular systems of male Wistar albino rats via ingestion of contaminated water. Four groups of six rats each were exposed to uncontaminated water (group A as control), 100 mg/L of Cd (group B), 100 mg/L of Ni (group C), and a combination of 100 mg/L Cd and Ni (group D) for 28 days. The stomach, intestinal, and ocular weights were recorded and were significantly reduced (p ≤ 0.05) in the treatment groups when compared to the control, but the effect was profound in group D rats, as compared with groups B and C rats. The assessed amylase, lipase, and alkaline phosphatase (ALP) activities were significantly impaired (p > 0.05) in the tissues with some exceptions among treatment groups relative to the control. The increased stomach, intestinal, and ocular malondialdehyde (MDA) levels (p ≤ 0.05) affirmed the induction of oxidative stress among treatment groups with depleted antioxidant defense system in assessed tissues, but no significant change (p > 0.05) in ocular reduced glutathione (GSH) level was observed, as compared with the control. The tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) levels assessed were significantly elevated (p ≤ 0.05) in the tissues of groups B and D rats with some exceptions among group C rats when compared to the control. The histological alteration characterized by cellular degeneration, oxidative damage, inflammation, and tissue necrosis further affirmed the synergistic impacts with indications of gastritis, retinal dysfunction, and ocular impairment in predisposed rats.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship Between Magnesium Depletion Score and Kidney Stone Risk in Gout Patients: a Mediation Analysis.","authors":"Jing Liu, Yu Lei","doi":"10.1007/s12011-025-04638-6","DOIUrl":"https://doi.org/10.1007/s12011-025-04638-6","url":null,"abstract":"<p><p>This study investigated the association and mediating mechanisms between magnesium depletion score (MDS) and kidney stones (KDS) in gout patients through a cross-sectional analysis. Logistic regression analysis of 502 gout patients revealed a significant positive association between MDS grade and KDS (OR = 2.14, 95% CI: 1.78-2.58), with the risk remaining elevated in the MDS4 group after adjusting for confounders (OR = 6.25, 95% CI: 2.38-16.44).Causal mediation analysis indicated that MDS fully explained the effect of serum uric acid (SUA) on KDS, suggesting that disrupted magnesium metabolism plays a key role in gout-related kidney injury. Subgroup analysis revealed an increased risk of KDS in obese, hypertensive, and diabetic patients with MDS. MDS should be incorporated into clinical risk assessments for gout, and magnesium supplementation or combined uric acid-lowering therapy should be recommended for high-risk groups to prevent KDS.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Concentrations of Selected Trace Elements (Cd, Pb, As, Zn, Fe) in Traditional Food Products and in Food Products Containing House Crickets (Acheta domesticus)-Preliminary Study.","authors":"Monika Rusin, Anna Dziekan, Joanna Domagalska, Weronika Osmala-Kurpiewska","doi":"10.1007/s12011-025-04624-y","DOIUrl":"https://doi.org/10.1007/s12011-025-04624-y","url":null,"abstract":"<p><p>The acceleration of global population growth will consequently result in a surge of demand for food. The cultivation of insects for human consumption represents a significant method of satisfying human nutritional requirements. The aim of this study is to analyze the concentration of selected metals, namely iron, zinc, lead, cadmium, and arsenic, in traditional food products and to compare them with the concentrations of these trace elements in food products containing house crickets. The samples of traditional food products (n = 27) and food products containing house crickets (n = 30) were classified to the food categories: flour, pasta, crisps, bars, chocolate, crisp bread, crackers, bread spreads, bread sticks, and dried snacks. The concentration of lead, cadmium, zinc, arsenic, and iron was determined through the use of inductively coupled plasma optical emission spectrometry (ICP-OES) and electrothermal atomic absorption spectrometry (ET-AAS). The number of scientific reports on the assessment of the health risk associated with dietary exposure to metals through the consumption of food containing edible insects is relatively limited. The results of the present study demonstrate that products containing house crickets exhibit higher concentrations of zinc, iron, and arsenic than traditional products (Mann-Whitney U test). The R-Spearman rank correlation test revealed a statistically significant correlation between the percentage content of house crickets in food products and the concentrations of zinc and iron. The results of the study indicate a positive correlation between the percentage of house crickets in food products and the concentration of metals.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Subchronic Manganese and Iron Exposure, Alone or in Combination, on Elemental Distribution in Rats.","authors":"Yan Li, Jian-Chao Peng, Yuan-Yuan Fang, Li-Mei Qin, Michael Aschner, Yue-Ming Jiang","doi":"10.1007/s12011-025-04626-w","DOIUrl":"https://doi.org/10.1007/s12011-025-04626-w","url":null,"abstract":"<p><p>Overexposure to manganese (Mn) or iron (Fe) may lead to neurological damage. The aim of this study was to investigate the effects of subchronic Mn and Fe exposure, alone or in combination, on the distribution of other elements and the relationship between Mn and Fe levels in whole blood and brain. Forty male Sprague-Dawley (SD) rats were divided into control, Mn-exposed, Fe-exposed, and combined Mn-Fe-exposed groups, with 10 rats assigned randomly to each group. The control, Mn-exposed, Fe-exposed group and the combined Mn-Fe-exposed groups were injected intraperitoneally with equal amounts of saline, 5 mg/kg MnCl₂, 20 mg/kg FeSO₄ or 5 mg/kg MnCl₂+20 mg/kg FeSO₄ once a day, 5 days a week for 8 weeks. The levels of Mn, Fe and other metallic elements [including barium (Ba), beryllium (Be), strontium (Sr), antimony (Sb), lead (Pb), vanadium (V) and copper (Cu)] in whole blood and brain tissue (including the globus pallidus, hippocampus, striatum and substantia nigra) were determined by inductively coupled plasma-mass spectrometry (ICP-MS). The results of this study show that, in whole blood, Mn levels were increased (p < 0.05) in the Mn-exposed group, Fe levels were decreased in both the Fe-exposed (p < 0.05) and Mn-exposed groups (p < 0.01), and Sb levels were increased in both the Mn-exposed and combined Mn-Fe-exposed groups (p < 0.05). In the substantia nigra, the levels of Be (p < 0.01), Sr (p < 0.05), and Cu (p < 0.001) were increased in the Fe-exposed group; the levels of Cu were also significantly increased in the Mn-exposed group (p < 0.01) and the combined Mn-Fe-exposed group (p < 0.0001); the levels of V were decreased (p < 0.05) in the combined Mn-Fe-exposed group; and the levels of V were decreased in the Fe-exposed group (p < 0.01 ), Mn-exposed group (p < 0.05) and combined Mn-Fe-exposed group (p < 0.001) had decreased Ba levels. In the pallidum, Fe levels were increased in the Mn-Fe co-exposed group (p < 0.0001); Ba (p < 0.01) and Pb (p < 0.05) levels were decreased in the Fe-exposed group; Ba (p < 0.05) levels were decreased in the Mn-exposed group; and Ba levels were increased in the Mn-Fe co-exposed group (p < 0.05). In the hippocampus, Mn (p < 0.01), Cu (p < 0.05), Sb (p < 0.01), and V (p < 0.05) levels were increased in the Fe-exposed group; Mn levels were increased in the Mn-exposed group (p < 0.01) and the combined Mn-Fe-exposed group (p < 0.0001). In the striatum, Be levels were decreased in the Mn-Fe combined exposure group (p < 0.05). Mn and Fe levels in whole blood and brain tissue can reflect the accumulation of Mn and Fe. These measurements can serve as valuable predictive biomarkers for subchronic Mn or Fe exposure and combined Mn-Fe exposure. The interactions between Mn and Fe and the distribution and abnormalities of the essential metal elements in the central nervous system and other organs need to be further investigated.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}