Biomeditsinskaya khimiya最新文献

{"title":"Efficacy of poloxamer Р188 in restoring the metabolic profile in myelosuppression: a non-invasive nuclear magnetic resonance spectroscopy study.","authors":"A V Kholina, N A Borozdina, V A Palikov, I A Dyachenko, I N Kravchenko, R A Dalevich, I A Pakhomova, E N Kazakova, N M Pankratova, A N Pankratov, N V Pozdnyakov, A N Murashev, E V Arshintseva, S Yu Pushkin, M V Molchanov, V Yu Ziamko, M A Timchenko","doi":"10.18097/PBMCR1662","DOIUrl":"10.18097/PBMCR1662","url":null,"abstract":"<p><p>Modern diagnostics in oncohematology is based on highly informative \"gold standards\" such as bone marrow biopsy, immunophenotyping, and cytogenetic analysis. The need to monitor the progression of the disease and the effectiveness of therapy requires the development of additional noninvasive approaches. One such approach is nuclear magnetic resonance spectroscopy (¹H NMR spectroscopy); its key advantage is the ability to quickly and simultaneously identify many metabolites in biological body fluids to construct clinically relevant metabolomic profiles and provide an integrated picture of metabolic processes. The aim of the study was to identify key plasma metabolites altered due to carboplatin toxicity in the bone marrow of CBA mice and to evaluate the possibility of correcting these changes by oral administration of poloxamer P188. The animals were divided into 6 groups: intact control (CTR), a group with a pathology model without treatment (MYS), and 4 experimental groups receiving poloxamer P188 at doses from 10 mg/kg to 1000 mg/kg for 7 or 21 days. The plasma metabolic profile was analyzed by ¹H NMR spectroscopy on days 8 and 22. A total of 36 metabolites were identified in plasma by ¹H NMR spectroscopy, with statistically significant differences between groups found only for 15 compounds. A single intraperitoneal administration of carboplatin at a dose of 100 mg/kg caused significant metabolic shifts in the plasma of CBA mice associated with the development of myelosuppression: an increase in the concentrations of D-glucose-1-phosphate and lysine and a decrease in dimethylglycine, β-hydroxybutyrate, isobutyrate, and trimethylamine. Daily oral administration of P188 for 7 and 21 days in the myelosuppression model significantly affected the concentrations of key metabolites, resulting in increased isobutyrate and trimethylamine levels and decreased lysine levels. Thus, ¹H NMR spectroscopy enabled us to identify characteristic metabolic disturbances in carboplatin-induced myelosuppression in CBA mice and demonstrate the efficacy of P188, which normalized key metabolites: isobutyrate, trimethylamine, and lysine. These data confirm the potential of using NMR metabolomics for noninvasive and personalized monitoring of therapy efficacy.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 3","pages":"207-219"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148434867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA: role in pathogenesis, problems of microRNA therapy, and future prospects.","authors":"E V Kugaevskaya, O S Timoshenko, T A Gureeva, A V Lisitsa","doi":"10.18097/PBMCR1705","DOIUrl":"10.18097/PBMCR1705","url":null,"abstract":"<p><p>MicroRNAs are small non-coding RNA molecules of 20-24 nucleotides in length. They play a key role in regulation of gene expression by influencing stability and translation of mRNA in a wide range of biological processes. Since their discovery, a large number of microRNAs have been described, and significant progress has been made in elucidating their functions. Convincing evidence now exists that microRNAs are powerful genetic regulators. The discovery of a link between microRNAs and numerous human diseases, particularly various types of cancer, stimulated a significant interest in exploring their therapeutic potential. They represent a new class of therapeutics capable of restoring impaired cellular functions, especially in various malignancies. Despite significant progress in preclinical research, microRNA-based therapy is still in the beginning stages; only a few microRNAs have been taken into further clinical trials. This is due to their off-target effects, primarily toxicity and immunogenicity, which significantly limit the use of microRNAs as therapeutic agents. One way to overcome off-target effects is to develop new systems for targeted delivery of microRNAs to the target tissue (disease site). Managing the off-target effects of microRNAs is a serious problem that requires solutions.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 3","pages":"157-187"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148434976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of proteins with altered PTM levels in patients with temporal lobe epilepsy in different regions of the human brain.","authors":"Yu V Miroshnichenko, A V Rybina, V S Skvortsov","doi":"10.18097/PBMCR1641","DOIUrl":"10.18097/PBMCR1641","url":null,"abstract":"<p><p>In this study we have reanalyzed the original proteomic data from studies of hippocampal [DOI: 10.1172/jci.insight.188612] and temporal cortex [DOI: 10.1016/j.expneurol.2025.115361] tissues of patients with temporal lobe epilepsy. The goal was to identify proteins whose levels of post-translational modifications (PTMs) could be altered in this disease. Three datasets (PXD064519, ZMP8KU, and PXD010154) deposited at PRIDE and Texas Data Repository were used. Phosphorylation and N-terminal acetylation were considered as target PTMs. During re-identification of peptides we considered only peptides, for which both variants (with and without PTMs) were identified. A data comparison algorithm independent of experimental data alignment between individual samples was used. As a result, 35 proteins were selected that showed significant changes in phosphorylation levels. For 12 of these proteins, significant changes were recorded in both data sets. Twenty-nine proteins from this list have been linked to epilepsy pathogenesis in the literature.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 2","pages":"118-127"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of the physical exercise-induced increase of brain-derived neurotrophic factor levels: a systematic review.","authors":"J C Wibawa, N Ayubi, B N Putro, M Kurnaz","doi":"10.18097/PBMCE0039","DOIUrl":"10.18097/PBMCE0039","url":null,"abstract":"<p><p>Physical inactivity triggers several metabolic syndromes and influences cognitive function, including the development of dementia. Exercise can help to prevent these negative effects. However, there is currently limited research examining how exercise affects cognitive function through brain-derived neurotrophic factor (BDNF) levels, and the underlying mechanisms remain unclear. The aim of this study was to analyze existing literature on the effect of physical exercise on brain-derived neurotrophic factor (BDNF) levels as a biomarker of cognitive function. Several journal databases, including Scopus, Web of Science, PubMed, and Science Direct, were searched for this study. The study considered several variables, including studies on BDNF, high-intensity exercise, and moderate-intensity exercise published within the last ten years. Articles that did not meet the inclusion criteria (e.g. animal studies) were excluded from this systematic review. Using databases from PubMed, Science Direct, Web of Science, and Scopus, a total of 152 publications were identified. Ten carefully selected, peer-reviewed articles addressed the need for this systemic change. Standard operating procedures for this study were established using the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines. Based on the results of this comprehensive study, it is evident that exercise increases BDNF levels in humans. Although high-intensity exercise is more effective in increasing BDNF levels in humans than moderate-intensity exercise, further research is needed for selection of the optimal physical load required for BDNF expression. Physical exercise is recommended to improve brain development and memory ability.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 2","pages":"102-111"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic acid: biochemical properties and medical applications.","authors":"V V Shumyantseva, L E Agafonova, T A Filippova, R A Masamrekh, Yu Yu Khudoklinova, A V Kuzikov, A I Archakov","doi":"10.18097/PBMCR1645","DOIUrl":"10.18097/PBMCR1645","url":null,"abstract":"<p><p>This literature review examines the biological properties of hyaluronic acid (HA), its various chemical modifications with carboxyl, hydroxyl, and acetamide groups, applicable in drug and genetic material delivery systems. Special attention is paid to the use of HA in complexes with metal nanoparticles, other biopolymers, and biomolecules. HA molecules of different molar masses exhibit different effects on cellular processes. Therefore, HA fractions with strictly defined molecular masses are used to achieve various goals. Unique properties of HAsuch as high bioavailability, biocompatibility, antioxidant properties, and high affinity for a number of cellular receptors make HA a promising means for use in targeted therapy. The use of HA in regenerative medicine has been also discussed.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 2","pages":"85-101"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of antibodies to study cardiac renalase levels in the normotensive and hypertensive rats.","authors":"V I Fedchenko, O A Buneeva, S A Kaloshina, A T Kopylov, M V Medvedeva, A E Medvedev","doi":"10.18097/PBMCR1668","DOIUrl":"10.18097/PBMCR1668","url":null,"abstract":"<p><p>Renalase (RNLS) is a protein that plays an important role in the regulation of blood pressure in humans and animals. Previously, we found higher levels of RNLS mRNA in the hearts of spontaneously hypertensive rats (SHR rats) compared to normotensive controls (WKY - Wistar Kyoto rats) (Fedchenko et al., 2013; Med. Sci. Monit. Basic Res., 19, 267-270). In this study, we assessed the RNLS protein level in the hearts of these animals by means of available poly- and monoclonal antibodies to human and rat RNLS. Western blot analysis revealed an increase in several protein bands within the molecular mass range of RNLS from various sources (35-40 kDa). However, mass spectrometry analysis did not detect the presence of RNLS, and other rat heart proteins were detected in the protein bands of varying intensity: lactate dehydrogenase (LDHB; P42123) and mitochondrial malate dehydrogenase (MDHM; P04636). The interaction of highly purified LDH with anti-RNLS antibodies was confirmed in direct experiments using Western blot analysis. These results indicate that some cardiac proteins can (non-specifically?) interact with anti-RNLS antibodies. This significantly limits their use and requires detailed verification of the obtained results using more specific (alternative) molecular tools.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 2","pages":"112-117"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of levodopa on the blood antioxidant system and metabolome in patients with Parkinson's disease.","authors":"D A Abaimov, T N Fedorova, A A Shabalina, A S Silantyev, D N Konanov, E Yu Fedotova, V V Poleshchuk, M N Andreev, O V Trifonova, D L Maslov, E E Balashova, D A Sychev, S N Illarioshkin, P G Lokhov","doi":"10.18097/PBMCR1642","DOIUrl":"10.18097/PBMCR1642","url":null,"abstract":"<p><p>The blood serum antioxidant status and the blood plasma metabolomic profile have been analyzed in Parkinson's disease (PD) patients, both pharmacologically naive and taking levodopa (treatment duration 7.4±5.4 years) at low (< 300 mg/day) and high (> 300 mg/day) doses. High-dose levodopa therapy caused changes in both the antioxidant status and metabolome of patients. High-dose levodopa treatment also activated lipid peroxidation, manifested by an increase in the level of lipid hydroperoxides. High-resolution mass spectrometry revealed accumulation of levodopa and dopamine metabolic products in the metabolome, particularly the neurotoxic dopamine metabolite 3,4-dihydroxyphenylacetaldehyde (DOPAL), and associated changes in related metabolic pathways. Low-dose levodopa treatment did not cause significant changes in the antioxidant status and metabolome of patients, thus suggesting that maintaining a low-dose regimen as long as possible could potentially reduce neurotoxic metabolite accumulation and also maintain normal antioxidant functions in PD patients.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 2","pages":"141-151"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pilot shotgun proteomic profiling of peripheral blood mononuclear cells in patients with different MRI phenotypes of cerebral small vessel disease.","authors":"A A Geints, P S Shlapakova, L A Dobrynina, E I Kremneva, E V Khriapova, V G Zgoda, O V Tikhonova","doi":"10.18097/PBMCR1643","DOIUrl":"10.18097/PBMCR1643","url":null,"abstract":"<p><p>Age-related cerebral microangiopathy (CMA) also known as cerebral small vessel disease (CSVD), is a leading cause of cognitive impairment and stroke. Difficulties in studying CSVD are associated with limitations in visualizing small vessels and diagnostics based on MRI signs of brain damage (white matter hyperintensity, lacunae, microbleeds, etc.). Our previous assessment of each CSVD MRI feature using a four-point severity scale and distribution among brain region using cluster analysis revealed the existence of two MRI types. They do not differ in the severity of vascular risk factors but do differ in the severity of clinical manifestations and levels of circulating plasma biomarkers. Here we present results of a pilot panoramic study of the proteome of peripheral blood mononuclear cells from patients with CSVD MRI types I and II, as well as healthy volunteers. CSVD patients showed a tendency toward downregulation of proteins associated with vesicular trafficking and extracellular matrix (ECM) remodeling relative to control values. Patients with CSVD MRI type 1 showed trends toward insufficient activation of protective proteins (arginase-1, thioredoxin, autophagy and protein stress regulators) and excessive activation of platelet proteins and vascular wall remodeling regulators (such as profilin-1), compared to patients with CSVD MRI type 2. These results indicate the need to study the microstructure of the basement membrane, vascular ECM, and perivascular spaces in cerebral small vessels.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 2","pages":"128-140"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of the secretory phospholipases A2 from Gloydius blomhoffii and Gloydius halys venoms on hemostasis components in vitro.","authors":"M A Solovyeva, L V Galebskaya, M A Galkin, G M Berdichevskiy, E V Semenova, I M Mikhel, E A Rogozhin, L V Vasina","doi":"10.18097/PBMCR1639","DOIUrl":"10.18097/PBMCR1639","url":null,"abstract":"<p><p>The aim of this study was to investigate the effects of Gloydius blomhoffii and Gloydius halys venoms and their fractions on platelet and plasma hemostasis, and to evaluate the ability of the sPLA2 inhibitor Varespladib to neutralize the coagulopathic activity mediated by secretory phospholipase A2 (sPLA2) present in these venoms. The proaggregatory effect of whole G. halys and G. blomhoffii venoms on platelet hemostasis was associated with multidirectional effects of their multiple protein components, while sPLA2-containing fractions exhibited an antiaggregatory effect. Whole G. halys and G. blomhoffii venoms and Varespladib added to them did not affect the extrinsic pathway of hemocoagulation, but inhibited the intrinsic pathway of human blood coagulation. The paradoxical effect of further enhancing the anticoagulant activity of G. halys and G. blomhoffii venoms with the sPLA2 inhibitor Varespladib could be determined by a stronger sPLA2 interaction with blood coagulation factor Xa after formation of the enzyme-inhibitor complex.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 1","pages":"21-27"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The VPS35 protein and the role of its impairments in mitochondrial dysfunction in Parkinson's disease.","authors":"O A Buneeva, A E Medvedev","doi":"10.18097/PBMCR1648","DOIUrl":"10.18097/PBMCR1648","url":null,"abstract":"<p><p>The VPS35 is an essential protein that plays multifunctional roles in various biological processes. It is a core component of the retromer complex, involved in protein recycling from endosomes to the trans-Golgi network (TGN) and the plasma membrane. Besides its role as the retromer complex component, VPS35 interacts with many proteins and regulates mitochondrial homeostasis, mitochondrial dynamics (fusion and fission), and other important processes in various cell compartments. In the context of Parkinson's disease (PD) convincing evidence exists that VPS35 mutations, particularly [D620N], have a significant impact on normal retromer functioning, mitochondrial dysfunction, and impairment of neuronal health and survival. In this review we briefly consider structure and functions of the retromer complex, the role of VPS35 in mitochondria, and finally analyze physical and functional interactions of this protein with PD-important proteins associated with mitochondria.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 1","pages":"5-20"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}