脊髓灰质炎灭活疫苗减毒株与抗体相互作用的SPR分析。

Q3 Biochemistry, Genetics and Molecular Biology

Biomeditsinskaya khimiya Pub Date : 2025-02-01 DOI:10.18097/PBMCR1507

O V Gnedenko, Yu Yu Ivin, A N Piniaeva, A N Zyrina, I V Levin, N S Borisenko, D D Zhdanov, A S Ivanov, A V Lisitsa, A A Ishmukhametov, A I Archakov

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引用次数: 0

摘要

研究了灭活脊髓灰质炎病毒疫苗株与通过Fc片段固定在蛋白A上的定向抗体的相互作用。采用SPR生物传感器测定了不同灭活方法灭活的Sabin 1型和2型疫苗减毒脊髓灰质炎病毒相互作用的动力学和平衡参数。Sabin株2型脊髓灰质炎病毒多克隆抗体与β-丙内酯灭活的Sabin株2型脊髓灰质炎病毒的相互作用最强，KD = 1.04⋅10-11 M， Sabin株1型脊髓灰质炎病毒单克隆抗体与甲醛灭活的Sabin株1型脊髓灰质炎病毒的相互作用最强。KD = 1.39⋅10-11 M. Sabin株1型和2型灭活疫苗脊髓灰质炎病毒与固定化抗体的高亲和相互作用表明，用β-丙内酯或甲醛灭活病毒后，d抗原保留了其结构。

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The SPR analysis of the interaction of inactivated poliovirus vaccine attenuated strains with antibodies.

The interaction of inactivated poliovirus vaccine strains with oriented antibodies immobilized to protein A via Fc fragments has been investigated. Using an SPR biosensor, the kinetic and equilibrium parameters of the interaction of vaccine attenuated polioviruses of the Sabin strains type 1 and type 2, inactivated by various methods were determined. The strongest interaction was observed between polyclonal antibodies to Sabin strain type 2 poliovirus and Sabin strain type 2 poliovirus inactivated with β-propiolactone, KD = 1.04⋅10-11 M, as well as the interaction of monoclonal antibodies to Sabin strain type 1 poliovirus and Sabin strain type 1 poliovirus inactivated with formaldehyde, KD = 1.39⋅10-11 M. The high-affinity interaction of inactivated vaccine polioviruses of the Sabin strains type 1 and type 2 with immobilized antibodies indicates that the D-antigen retained its structure after virus inactivation with β-propiolactone or formaldehyde.

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来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).