Biomeditsinskaya khimiya最新文献

The effect of the secretory phospholipases A2 from Gloydius blomhoffii and Gloydius halys venoms on hemostasis components in vitro. 黄颡鱼和黄颡鱼毒液分泌磷脂酶A2对体外止血成分的影响。

Biomeditsinskaya khimiya Pub Date : 2026-02-01 DOI: 10.18097/PBMCR1639

M A Solovyeva, L V Galebskaya, M A Galkin, G M Berdichevskiy, E V Semenova, I M Mikhel, E A Rogozhin, L V Vasina

{"title":"The effect of the secretory phospholipases A2 from Gloydius blomhoffii and Gloydius halys venoms on hemostasis components in vitro.","authors":"M A Solovyeva, L V Galebskaya, M A Galkin, G M Berdichevskiy, E V Semenova, I M Mikhel, E A Rogozhin, L V Vasina","doi":"10.18097/PBMCR1639","DOIUrl":"https://doi.org/10.18097/PBMCR1639","url":null,"abstract":"The aim of this study was to investigate the effects of Gloydius blomhoffii and Gloydius halys venoms and their fractions on platelet and plasma hemostasis, and to evaluate the ability of the sPLA2 inhibitor Varespladib to neutralize the coagulopathic activity mediated by secretory phospholipase A2 (sPLA2) present in these venoms. The proaggregatory effect of whole G. halys and G. blomhoffii venoms on platelet hemostasis was associated with multidirectional effects of their multiple protein components, while sPLA2-containing fractions exhibited an antiaggregatory effect. Whole G. halys and G. blomhoffii venoms and Varespladib added to them did not affect the extrinsic pathway of hemocoagulation, but inhibited the intrinsic pathway of human blood coagulation. The paradoxical effect of further enhancing the anticoagulant activity of G. halys and G. blomhoffii venoms with the sPLA2 inhibitor Varespladib could be determined by a stronger sPLA2 interaction with blood coagulation factor Xa after formation of the enzyme-inhibitor complex.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 1","pages":"21-27"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The VPS35 protein and the role of its impairments in mitochondrial dysfunction in Parkinson's disease. VPS35蛋白及其损伤在帕金森病线粒体功能障碍中的作用

Biomeditsinskaya khimiya Pub Date : 2026-02-01 DOI: 10.18097/PBMCR1648

O A Buneeva, A E Medvedev

引用次数: 0

Microbiome associated with recurrent vulvovaginal candidiasis: key characteristics and potential therapeutic targets. 与复发性外阴阴道念珠菌病相关的微生物组：关键特征和潜在的治疗靶点。

Biomeditsinskaya khimiya Pub Date : 2026-02-01 DOI: 10.18097/PBMCR1644

E G Oganesyan, A S Zhuk, V V Venchakova, Yu V Dolgo-Saburova, O N Zhorzh, F-M Zhang, N V Vasilyeva, A E Taraskina

{"title":"Microbiome associated with recurrent vulvovaginal candidiasis: key characteristics and potential therapeutic targets.","authors":"E G Oganesyan, A S Zhuk, V V Venchakova, Yu V Dolgo-Saburova, O N Zhorzh, F-M Zhang, N V Vasilyeva, A E Taraskina","doi":"10.18097/PBMCR1644","DOIUrl":"https://doi.org/10.18097/PBMCR1644","url":null,"abstract":"Recurrent vulvovaginal candidiasis (RVVC) is one of the most complex forms of urogenital infection in terms of its clinical burden, impact on quality of life, and difficulty in preventing relapses. The aim of this study was to comprehensively characterize the taxonomic composition and functional potential of the vaginal microbiome associated with RVVC. This case-control study included patients with RVVC and conditionally healthy women. Vaginal samples were analyzed using shotgun metagenomic sequencing, followed by taxonomic and functional annotation of the microbiome using data quality control, taxonomic classification (Kraken2, MetaPhlAn4), and functional annotation (HUMAnN 3.9). At the community structure level, the RVVC microbiome exhibited pronounced interindividual variability and did not represent a uniform microbiota configuration. The taxonomic profile of the microbiome in RVVC was characterized by an increased relative abundance of Lactobacillus iners and anaerobic taxa (Prevotella bivia, Dialister microaerophilus), forming a compact \"core\" of intergroup differences. Functional analysis revealed a limited but reproducible set of metabolic pathways associated with RVVC; these included pathways of purine metabolism, central carbohydrate metabolism, and biosynthesis of cofactors and cell wall components. RVVC is associated not only with changes in the taxonomic composition of the microbiota but also with a stable reconfiguration of its functional potential. The identified shifts in metabolic pathway patterns reflect a transition of the vaginal microbial community to an alternative functional state, thus highlighting the need to develop new therapeutic strategies alternative to traditional antifungal-based approaches.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 1","pages":"62-74"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous inhibition of mTOR and STING as an approach to reduce alpha-synuclein and lysosphingolipid levels in peripheral blood monocytederived macrophages and the SH-SY5Y cell line: implications for therapy of Parkinson's disease. 同时抑制mTOR和STING降低外周血单核源性巨噬细胞和SH-SY5Y细胞系中α -突触核蛋白和溶鞘脂水平：对帕金森病治疗的影响

Biomeditsinskaya khimiya Pub Date : 2026-02-01 DOI: 10.18097/PBMCR1616

A I Bezrukova, K S Basharova, E S Galkina, O S Epifanivskaya, G V Baydakova, E Yu Zakharova, S N Pchelina, T S Usenko

引用次数: 0

The effect of nortriptyline and its combination with mometasone on secretion of IL-6 and IL-8 by stimulated blood mononuclear cells in vitro. 去甲替林及其联用莫米松对体外刺激单核细胞分泌IL-6、IL-8的影响。

Biomeditsinskaya khimiya Pub Date : 2026-02-01 DOI: 10.18097/PBMCR1630

T V Mironova, A D Taganovich, V V Makarevich, T S Kolesnikova, O V Levandovskaya, I P Shilovsky, M R Khaitov, A G Kadushkin

{"title":"The effect of nortriptyline and its combination with mometasone on secretion of IL-6 and IL-8 by stimulated blood mononuclear cells in vitro.","authors":"T V Mironova, A D Taganovich, V V Makarevich, T S Kolesnikova, O V Levandovskaya, I P Shilovsky, M R Khaitov, A G Kadushkin","doi":"10.18097/PBMCR1630","DOIUrl":"https://doi.org/10.18097/PBMCR1630","url":null,"abstract":"Glucocorticosteroids (GCS) are often the medication of choice for chronic inflammation. Since not all patients are equally sensitive to GCS, certain efforts are undertaken to increase sensitivity and complement glucocorticosteroid therapy with other nonsteroidal medications that can enhance the anti-inflammatory effect of GCS. The tricyclic antidepressant nortriptyline has demonstrated anti-inflammatory properties in several experimental studies, as well as the ability to complement the action of GCS. The aim of this study was to investigate the effects of nortriptyline, as well as its combination with mometasone, on blood mononuclear cells (MNCs) under conditions of stimulated immune responses (IR) of types 1, 2, and 17. In isolated MNCs from six healthy donors, IR type I, type 2, or type 17 were stimulated in the presence of nortriptyline, mometasone, or their combination by adding recombinant activator proteins (IL-2, IL-25, IL-33, thymic stromal lymphopoietin (TSLP), IL-12, IL-1β, IL-23). After three days, the concentration of IL-6 and IL-8 was determined in the supernatants by enzyme-linked immunosorbent assay. In the presence of nortriptyline at a final concentration of 10-5 M, IR type 2 and type 17 were accompanied by a decrease in IL-6 concentration. Addition of a combination of mometasone and nortriptyline to the MNC culture medium under conditions of IR type II activation had a potentiating effect. This was evidenced by a decrease in IL-6 and IL-8 secretion compared to the use of mometasone alone. The study demonstrates the ability of nortriptyline to suppress the secretion of proinflammatory cytokines by blood cells, which is selective and dependent on the type of immune response.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"72 1","pages":"75-79"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147503149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astaxanthin protects heart mitochondria from damage caused by chronic alcohol intoxication. 虾青素保护心脏线粒体免受慢性酒精中毒造成的损害。

Biomeditsinskaya khimiya Pub Date : 2026-02-01 DOI: 10.18097/PBMCR1617

O V Krestinina, I V Odinokova, A I Zvyagina, R R Sotnikov, L D Sotnikova, Yu L Baburina

引用次数: 0

Circulating protein biomarkers of glioblastoma: methodologic approaches and perspectives of development. 胶质母细胞瘤的循环蛋白生物标志物：方法学方法和发展前景。

Biomeditsinskaya khimiya Pub Date : 2025-12-01 DOI: 10.18097/PBMCR1637

V A Maiorov, O V Tikhonova, V G Zgoda

引用次数: 0

The effect of copper ions on cultured rat glial cells of the cerebral cortex under the action of lipopolysaccharide. 铜离子在脂多糖作用下对培养大鼠大脑皮层胶质细胞的影响。

Biomeditsinskaya khimiya Pub Date : 2025-12-01 DOI: 10.18097/PBMCR1597

E V Stelmashook, E E Genrikhs, O P Alexandrova, A E Lapieva, M R Kapkaeva, N K Isaev

{"title":"The effect of copper ions on cultured rat glial cells of the cerebral cortex under the action of lipopolysaccharide.","authors":"E V Stelmashook, E E Genrikhs, O P Alexandrova, A E Lapieva, M R Kapkaeva, N K Isaev","doi":"10.18097/PBMCR1597","DOIUrl":"https://doi.org/10.18097/PBMCR1597","url":null,"abstract":"Copper ions (Cu2+) at concentrations of 25-50 μM stimulate lipopolysaccharide (LPS)-induced nitric oxide (NO) production in glial cell cultures derived from rat cerebral cortex and containing both astrocytes and microglia. Addition of a higher Cu2+ concentration (100 μM) during LPS stimulation did not significantly increase NO in the incubation medium, while 200 μM Cu2+ decreased this parameter compared to LPS. Cu2+ ions at these concentrations decreased viability of cultured cells. Apparently, the decrease in cell viability is not associated with nitrite accumulation, because the addition of even 100 μM sodium nitrite to the culture medium did not reduce cell viability or affect the cytotoxicity of Cu2+. The study of microglial cells (using the IBA1 marker) revealed that in LPS-treated cultures, microglia had a predominantly flattened amoeboid morphology, characteristic of activated microglia. The LPS treatment also increased the cell body profile area and perimeter. At a concentration of 25 μM, Cu2+ ions did not affect the morphological changes in microglia associated with the inflammatory phenotype. It is possible that the copper-induced increase in LPS-induced NO production is mediated by astrocytes.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"71 6","pages":"424-431"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic profiling of patients with sepsis-associated encephalopathy. 败血症相关脑病患者的代谢组学分析。

Biomeditsinskaya khimiya Pub Date : 2025-12-01 DOI: 10.18097/PBMCR1599

E D Kessenikh, K M Bykova, E A Murashko, Ya A Dubrovskii, V V Dorofeykov, I A Savvina

{"title":"Metabolomic profiling of patients with sepsis-associated encephalopathy.","authors":"E D Kessenikh, K M Bykova, E A Murashko, Ya A Dubrovskii, V V Dorofeykov, I A Savvina","doi":"10.18097/PBMCR1599","DOIUrl":"10.18097/PBMCR1599","url":null,"abstract":"Sepsis-associated encephalopathy (SAE) is a condition characterized by acute brain dysfunction developed in the absence of a primary infection in the central nervous system. The aim of this study was to perform a pilot, untargeted metabolomic profiling of the blood plasma of SAE patients to identify metabolic changes potentially associated with the pathological condition and to generate hypotheses for further studies of its pathogenesis, as well as to the search for promising biomarkers, and the assessment of the severity of the patient's condition. Metabolomic profiling was performed using HPLC-HR-MS, followed by statistical analysis of the obtained data. This blinded, randomized, controlled clinical trial revealed significant differences in the metabolic profiles of the study and control groups. Functional analysis showed the metabolic pathways most affected by pathological processes in SAE patients. These included metabolism of acylcarnitines, lysophosphatidylcholines, and taurine, folate biosynthesis, and the drug metabolism involving the cytochrome P450 pathway. In SAE patients with impaired consciousness, including delirium and coma, decreased levels of long-chain acylcarnitines and lysophosphatidylcholines were observed. The metabolomic profiles of SAE patients differed significantly between the groups of deceased and surviving patients: concentrations of sulfur-containing amino acids were significantly lower in the group of deceased than in the group of survivors. Our study identified 64 candidate biomarkers that could potentially be used to predict sepsis outcomes. However, further study is needed using an expanded and independent cohort of patients.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"71 6","pages":"441-453"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The myokine irisin: effects on the brain and therapeutic potential in the treatment of depression and neurodegenerative diseases. 肌因子鸢尾素：对大脑的影响和治疗抑郁症和神经退行性疾病的潜力。

Biomeditsinskaya khimiya Pub Date : 2025-12-01 DOI: 10.18097/PBMCR1640

A M Gisina, K N Yarygin

{"title":"The myokine irisin: effects on the brain and therapeutic potential in the treatment of depression and neurodegenerative diseases.","authors":"A M Gisina, K N Yarygin","doi":"10.18097/PBMCR1640","DOIUrl":"https://doi.org/10.18097/PBMCR1640","url":null,"abstract":"Epidemiological studies indicate a consistent global increase, including in the Russian Federation, in the number of patients with cognitive impairments associated with neurodegenerative diseases and various affective disorders. In this context there is a clear need in the development of more effective therapeutic approaches for their corrections. Good evidence exists that regular physical activity improves cognitive functions and alleviates depression. Working muscles secrete biologically active substances known as myokines, which regulate muscle recovery and functions of internal organs, endocrine glands, the immune system, and the brain. This results in a coordinated response of organs and systems aimed at restoring functional activity of the body after physical exercises and improves memory and learning ability. Patients with cognitive impairments or depression are often unable to engage in regular physical activity due to physical limitations or decreased motivation. Therefore, pharmaceuticals that mimic the effects of muscle activity are a promising therapeutic option. One potential direction in this field could be the development of drugs based on the myokine irisin, which is produced during physical exercise and exerts a range of beneficial effects on cognitive function and mood. This review summarizes existing data on the effects of physical exercise on cognitive function in health and disease; it describes the physiological effects of irisin, and presents the proposed mechanisms of irisin action on cognitive function and symptoms of depression.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"71 6","pages":"379-399"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0