The SPR analysis of the interaction of inactivated poliovirus vaccine attenuated strains with antibodies.

Q3 Biochemistry, Genetics and Molecular Biology

Biomeditsinskaya khimiya Pub Date : 2025-02-01 DOI:10.18097/PBMCR1507

O V Gnedenko, Yu Yu Ivin, A N Piniaeva, A N Zyrina, I V Levin, N S Borisenko, D D Zhdanov, A S Ivanov, A V Lisitsa, A A Ishmukhametov, A I Archakov

{"title":"The SPR analysis of the interaction of inactivated poliovirus vaccine attenuated strains with antibodies.","authors":"O V Gnedenko, Yu Yu Ivin, A N Piniaeva, A N Zyrina, I V Levin, N S Borisenko, D D Zhdanov, A S Ivanov, A V Lisitsa, A A Ishmukhametov, A I Archakov","doi":"10.18097/PBMCR1507","DOIUrl":null,"url":null,"abstract":"<p><p>The interaction of inactivated poliovirus vaccine strains with oriented antibodies immobilized to protein A via Fc fragments has been investigated. Using an SPR biosensor, the kinetic and equilibrium parameters of the interaction of vaccine attenuated polioviruses of the Sabin strains type 1 and type 2, inactivated by various methods were determined. The strongest interaction was observed between polyclonal antibodies to Sabin strain type 2 poliovirus and Sabin strain type 2 poliovirus inactivated with β-propiolactone, KD = 1.04⋅10-11 M, as well as the interaction of monoclonal antibodies to Sabin strain type 1 poliovirus and Sabin strain type 1 poliovirus inactivated with formaldehyde, KD = 1.39⋅10-11 M. The high-affinity interaction of inactivated vaccine polioviruses of the Sabin strains type 1 and type 2 with immobilized antibodies indicates that the D-antigen retained its structure after virus inactivation with β-propiolactone or formaldehyde.</p>","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"71 1","pages":"59-64"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomeditsinskaya khimiya","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18097/PBMCR1507","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

Abstract

The interaction of inactivated poliovirus vaccine strains with oriented antibodies immobilized to protein A via Fc fragments has been investigated. Using an SPR biosensor, the kinetic and equilibrium parameters of the interaction of vaccine attenuated polioviruses of the Sabin strains type 1 and type 2, inactivated by various methods were determined. The strongest interaction was observed between polyclonal antibodies to Sabin strain type 2 poliovirus and Sabin strain type 2 poliovirus inactivated with β-propiolactone, KD = 1.04⋅10-11 M, as well as the interaction of monoclonal antibodies to Sabin strain type 1 poliovirus and Sabin strain type 1 poliovirus inactivated with formaldehyde, KD = 1.39⋅10-11 M. The high-affinity interaction of inactivated vaccine polioviruses of the Sabin strains type 1 and type 2 with immobilized antibodies indicates that the D-antigen retained its structure after virus inactivation with β-propiolactone or formaldehyde.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).