s -亚硝基谷胱甘肽对HepG2细胞有机阴离子转运多肽1B1 （OATP1B1）表达及活性的诱导作用。

Q3 Biochemistry, Genetics and Molecular Biology

Biomeditsinskaya khimiya Pub Date : 2025-02-01 DOI:10.18097/PBMCR1487

O N Suchkova, Yu V Abalenikhina, A V Shchul'kin, P Yu Myl'nikov, F T Gadzhiyeva, P D Kochanova, M G Uzbekov, E N Yakusheva

{"title":"s -亚硝基谷胱甘肽对HepG2细胞有机阴离子转运多肽1B1 （OATP1B1）表达及活性的诱导作用。","authors":"O N Suchkova, Yu V Abalenikhina, A V Shchul'kin, P Yu Myl'nikov, F T Gadzhiyeva, P D Kochanova, M G Uzbekov, E N Yakusheva","doi":"10.18097/PBMCR1487","DOIUrl":null,"url":null,"abstract":"The effect of the nitric oxide donor S-nitrosoglutathione on the level and activity of organic anion transporting polypeptide 1B1 (OATP1B1), as well as the expression of the SLCO1B1 gene encoding the transporter protein, was investigated in HepG2 cells. The study has shown that treatment of cells with S-nitrosoglutathione for 3 h did not influence the content and activity of OATP1B1. Incubation with S-nitrosoglutathione (10-500 μM) for 24 h increased SLCO1B1 expression, the content of OATP1B1, and activity of the transporter protein. Induction of the OATP1B1 protein by the NO donor was suppressed by the soluble guanylate cyclase (sGC) inhibitor, 10 μM ODQ (1H-[1,2,4]oxadiazolo-[4,3-a]quinoxaline-1-OH). Thus, the study has shown that S-nitrosoglutathione, acting through the NO-sGC-cGMP signaling pathway, increased SLCO1B1 gene expression, accompanied by the increase in the transporter protein content and its activity in cells.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"71 1","pages":"29-36"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The inducing effect of S-nitrosoglutathione on the expression and activity of organic anion transporting polypeptide 1B1 (OATP1B1) in HepG2 cells.\",\"authors\":\"O N Suchkova, Yu V Abalenikhina, A V Shchul'kin, P Yu Myl'nikov, F T Gadzhiyeva, P D Kochanova, M G Uzbekov, E N Yakusheva\",\"doi\":\"10.18097/PBMCR1487\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The effect of the nitric oxide donor S-nitrosoglutathione on the level and activity of organic anion transporting polypeptide 1B1 (OATP1B1), as well as the expression of the SLCO1B1 gene encoding the transporter protein, was investigated in HepG2 cells. The study has shown that treatment of cells with S-nitrosoglutathione for 3 h did not influence the content and activity of OATP1B1. Incubation with S-nitrosoglutathione (10-500 μM) for 24 h increased SLCO1B1 expression, the content of OATP1B1, and activity of the transporter protein. Induction of the OATP1B1 protein by the NO donor was suppressed by the soluble guanylate cyclase (sGC) inhibitor, 10 μM ODQ (1H-[1,2,4]oxadiazolo-[4,3-a]quinoxaline-1-OH). Thus, the study has shown that S-nitrosoglutathione, acting through the NO-sGC-cGMP signaling pathway, increased SLCO1B1 gene expression, accompanied by the increase in the transporter protein content and its activity in cells.\",\"PeriodicalId\":8889,\"journal\":{\"name\":\"Biomeditsinskaya khimiya\",\"volume\":\"71 1\",\"pages\":\"29-36\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomeditsinskaya khimiya\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18097/PBMCR1487\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomeditsinskaya khimiya","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18097/PBMCR1487","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

摘要

研究了一氧化氮供体s -亚硝基谷胱甘肽对HepG2细胞有机阴离子转运多肽1B1 （OATP1B1）水平和活性以及编码转运蛋白的SLCO1B1基因表达的影响。研究表明，用s -亚硝基谷胱甘肽处理细胞3小时不影响OATP1B1的含量和活性。s -亚基谷胱甘肽（10-500 μM）孵育24 h，可提高SLCO1B1表达、OATP1B1含量和转运蛋白活性。可溶性鸟苷酸环化酶（sGC）抑制剂10 μM ODQ （1H-[1,2,4]oxadiazolo-[4,3-a]quinoxaline-1-OH）抑制NO供体对OATP1B1蛋白的诱导。因此，本研究表明，s -亚硝基谷胱甘肽通过NO-sGC-cGMP信号通路，使细胞内SLCO1B1基因表达增加，同时转运蛋白含量和活性增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The inducing effect of S-nitrosoglutathione on the expression and activity of organic anion transporting polypeptide 1B1 (OATP1B1) in HepG2 cells.

The effect of the nitric oxide donor S-nitrosoglutathione on the level and activity of organic anion transporting polypeptide 1B1 (OATP1B1), as well as the expression of the SLCO1B1 gene encoding the transporter protein, was investigated in HepG2 cells. The study has shown that treatment of cells with S-nitrosoglutathione for 3 h did not influence the content and activity of OATP1B1. Incubation with S-nitrosoglutathione (10-500 μM) for 24 h increased SLCO1B1 expression, the content of OATP1B1, and activity of the transporter protein. Induction of the OATP1B1 protein by the NO donor was suppressed by the soluble guanylate cyclase (sGC) inhibitor, 10 μM ODQ (1H-[1,2,4]oxadiazolo-[4,3-a]quinoxaline-1-OH). Thus, the study has shown that S-nitrosoglutathione, acting through the NO-sGC-cGMP signaling pathway, increased SLCO1B1 gene expression, accompanied by the increase in the transporter protein content and its activity in cells.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).