Biomeditsinskaya khimiya最新文献_第3页

The effect of lactoferin on the free radical and cytokine status of cornea in the experimental thermal burn. 乳铁蛋白对实验性热烧伤角膜自由基和细胞因子状态的影响

Biomeditsinskaya khimiya Pub Date : 2024-06-01 DOI: 10.18097/PBMC20247003168

I V Kirsanova, A V Kolesnikov, A V Shchulkin, Yu V Abalenikhina, P D Erokhina, E N Yakusheva

{"title":"The effect of lactoferin on the free radical and cytokine status of cornea in the experimental thermal burn.","authors":"I V Kirsanova, A V Kolesnikov, A V Shchulkin, Yu V Abalenikhina, P D Erokhina, E N Yakusheva","doi":"10.18097/PBMC20247003168","DOIUrl":"https://doi.org/10.18097/PBMC20247003168","url":null,"abstract":"The free radical and cytokine statuses of the cornea during its thermal burn and the possibility of its correction by lactoferrin have been studied in Soviet Chinchilla rabbits. The development of a corneal thermal burn was accompanied by the development of oxidative stress (increased levels of TBA-reactive substances and carbonyl derivatives of proteins, decreased activity of SOD and GPx enzymes) and a pronounced inflammatory reaction with increased levels of TNF-1α, IL-10, TGF-1β. The use of lactoferrin had a pronounced therapeutic effect, which was manifested by accelerated healing, prevention of the development of complications (corneal perforations), a decrease in the severity of oxidative stress, an increase in the concentrations of TNF-1α (in the early stages), IL-10 (in the later stages), TGF-1β (throughout the experiment). At the same time, by the end of regeneration more severe corneal opacification was recognized compared to the control group. This may be associated with an increased level of anti-inflammatory cytokines, especially TGF-1β.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"70 3","pages":"168-175"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The structural analogue of apelin-12 prevents energy disorders in the heart in experimental type 1 diabetes mellitus. 凋亡素-12的结构类似物可预防实验性1型糖尿病患者心脏能量紊乱。

Biomeditsinskaya khimiya Pub Date : 2024-06-01 DOI: 10.18097/PBMC20247003135

I M Studneva, O M Veselova, I V Dobrokhotov, L I Serebryakova, M E Palkeeva, D V Avdeev, A S Molokoedov, M V Sidorova, O I Pisarenko

{"title":"The structural analogue of apelin-12 prevents energy disorders in the heart in experimental type 1 diabetes mellitus.","authors":"I M Studneva, O M Veselova, I V Dobrokhotov, L I Serebryakova, M E Palkeeva, D V Avdeev, A S Molokoedov, M V Sidorova, O I Pisarenko","doi":"10.18097/PBMC20247003135","DOIUrl":"https://doi.org/10.18097/PBMC20247003135","url":null,"abstract":"Type 1 diabetes mellitus (T1DM) is the most severe form of diabetes, which is characterized by absolute insulin deficiency induced by the destruction of pancreatic beta cells. The aim of this study was to evaluate the effect of a structural analogue of apelin-12 ((NαMe)Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Nle-Pro-Phe-OH, metilin) on hyperglycemia, mitochondrial (MCh) respiration in permeabilized cardiac left ventricular (LV) fibers, the myocardial energy state, and cardiomyocyte membranes damage in a model of streptozotocin (STZ) diabetes in rats. Metilin was prepared by solid-phase synthesis using the Fmoc strategy and purified using HPLC. Four groups of animals were used: initial state (IS); control (C), diabetic control (D) and diabetic animals additionally treated with metilin (DM). The following parameters have been studied: blood glucose, MCh respiration in LV fibers, the content of cardiac ATP, ADP, AMP, phosphocreatine (PCr) and creatine (Cr), the activity of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) in blood plasma. Administration of metilin to STZ-treated rats decreased blood glucose, increased state 3 oxygen consumption, the respiratory control ratio in MCh of permeabilized LV fibers, and increased the functional coupling of mitochondrial CK (mt-CK) to oxidative phosphorylation compared with these parameters in group D. In STZ-treated animals metilin administration caused an increase in the PCr content and prevention of the loss of total creatine (ΣCr=PCr+Cr) in the diabetic hearts, as well as restoration of the PCr/ATP ratio in the myocardium and a decrease in the activity of CK-MB and LDH in plasma to initial values. Thus, metilin prevented energy disorders disturbances in cardiomyocytes of animals with experimental T1DM.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"70 3","pages":"135-144"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical profiling of poliovirus particles inactivated by chemical method and ionizing radiation. 用化学方法和电离辐射灭活脊髓灰质炎病毒颗粒的电化学分析。

Biomeditsinskaya khimiya Pub Date : 2024-06-01 DOI: 10.18097/PBMC20247003161

L E Agafonova, V V Shumyantseva, Yu Yu Ivin, A N Piniaeva, A A Kovpak, A A Ishmukhametov, S V Budnik, R S Churyukin, D D Zhdanov, A I Archakov

{"title":"Electrochemical profiling of poliovirus particles inactivated by chemical method and ionizing radiation.","authors":"L E Agafonova, V V Shumyantseva, Yu Yu Ivin, A N Piniaeva, A A Kovpak, A A Ishmukhametov, S V Budnik, R S Churyukin, D D Zhdanov, A I Archakov","doi":"10.18097/PBMC20247003161","DOIUrl":"https://doi.org/10.18097/PBMC20247003161","url":null,"abstract":"Electrochemical profiling of formaldehyde-inactivated poliovirus particles demonstrated a relationship between the D-antigen concentration and the intensity of the maximum amplitude currents of the poliovirus samples. The resultant signal was therefore identified as electrochemical oxidation of the surface proteins of the poliovirus. Using registration of electrooxidation of amino acid residues of the capsid proteins, a comparative electrochemical analysis of poliovirus particles inactivated by electrons accelerated with doses of 5 kGy, 10 kGy, 15 kGy, 25 kGy, 30 kGy at room temperature was carried out. An increase in the radiation dose was accompanied by an increase in electrooxidation signals. A significant increase in the signals of electrooxidation of poliovirus capsid proteins was detected upon irradiation at doses of 15-30 kGy. The data obtained suggest that the change in the profile and increase in the electrooxidation signals of poliovirus capsid proteins are associated with an increase in the degree of structural reorganization of surface proteins and insufficient preservation of the D-antigen under these conditions of poliovirus inactivation.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"70 3","pages":"161-167"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered serum levels of neuronal proteins and antibodies to them in occupational diseases of the nervous system. 神经系统职业病患者血清中神经元蛋白及其抗体水平的变化。

Biomeditsinskaya khimiya Pub Date : 2024-04-01 DOI: 10.18097/PBMC20247002109

G M Bodienkova, E V Boklazhenko

{"title":"Altered serum levels of neuronal proteins and antibodies to them in occupational diseases of the nervous system.","authors":"G M Bodienkova, E V Boklazhenko","doi":"10.18097/PBMC20247002109","DOIUrl":"https://doi.org/10.18097/PBMC20247002109","url":null,"abstract":"Aclinical and immunological examination of men with occupational pathology, including vibration disease (VD), occupational sensorineural hearing loss (SHL), and chronic mercury intoxication (CMI), was carried out. The comparison group consisted of men comparable in age and total work experience. Serum concentrations of neurotrophins (S100β, MBP, BDNF) and antibodies (ABs) to S100β and MBP proteins were determined by enzyme-linked immunosorbent assay. An increase in the level of the S100β protein was shown in CMI, VD, and a tendency for its increase was found in SHL. In parallel, an increase in AB to the S100β protein in VD and SHL and a decrease in AB in CMI were noted. A comparative assessment of MBP levels indicated a pronounced increase in its serum concentrations in patients with CMI and VD versus the comparison group. At the same time, an increase in the level of serum ABs to MBP in individuals with VD and SHL, and a decrease in patients with CMI were noted. In patients with CMI, a significant decrease in the BDNF concentration was found, while in SHL and VD, no statistically significant differences were found in comparison with the comparison group. The results obtained confirm importance of assessing serum concentrations of neurotrophic proteins and ABs to them in the case of occupational damage to the nervous system caused by exposure to physical and chemical factors.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"70 2","pages":"109-113"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of the levels and possible associations of alpha2-macroglobulin with autoantibodies in the serum of patients with various forms of autoimmune thyroiditis. 测定各种自身免疫性甲状腺炎患者血清中α2-巨球蛋白的水平及其与自身抗体的可能联系。

Biomeditsinskaya khimiya Pub Date : 2024-04-01 DOI: 10.18097/PBMC20247002125

R R Rahimova, A M Efendiyev, I J Shahverdiyeva, G S Dashdamirova, I A Kerimova

引用次数: 0

The effects of omega-3 fatty acids on antioxidant enzyme activities and nitric oxide levels in the cerebral cortex of rats treated ethanol. 欧米伽-3 脂肪酸对乙醇治疗大鼠大脑皮层抗氧化酶活性和一氧化氮水平的影响。

Biomeditsinskaya khimiya Pub Date : 2024-04-01 DOI: 10.18097/PBMC20247002083

Süleyman Oktar, Mahinur Karadeniz, Musa Acar, İsmail Zararsız

引用次数: 0

Assessment of platelet functional activity in healthy individuals and patients receiving antiplatelet therapy. Possible inconsistencies between aggregation and flow cytometry tests. 评估健康人和接受抗血小板治疗患者的血小板功能活性。聚集试验与流式细胞术试验之间可能存在的不一致。

Biomeditsinskaya khimiya Pub Date : 2024-04-01 DOI: 10.18097/PBMC20247002099

V V Bodrova, O N Shustova, N V Golubeva, A K Alieva, V V Vlodzyanovsky, D V Pevzner, A V Mazurov

{"title":"Assessment of platelet functional activity in healthy individuals and patients receiving antiplatelet therapy. Possible inconsistencies between aggregation and flow cytometry tests.","authors":"V V Bodrova, O N Shustova, N V Golubeva, A K Alieva, V V Vlodzyanovsky, D V Pevzner, A V Mazurov","doi":"10.18097/PBMC20247002099","DOIUrl":"https://doi.org/10.18097/PBMC20247002099","url":null,"abstract":"Platelet functional activity was assessed in healthy volunteers (HV, n=92), patients with stable angina pectoris (SA, n=42) and acute coronary syndrome (ACS, n=73), treated with acetylsalicylic acid (ASA) + clopidogrel and ASA + ticagrelor, respectively. In all HV and patients we have compared parameters of platelet aggregation (maximum light transmission and velocity, Tmax and Vmax) and parameters, characterizing exposure of platelet activation markers, evaluated by flow cytometry. HV platelets were activated by 10 μM, 1 μM TRAP, and 20 μM, 5 μM, 2.5 μM ADP; patient platelets were activated by 10 μM TRAP and by 20 μM and 5 μM ADP. Strong and significant correlations between the aggregation and flow cytometry parameters (the r correlation coefficient from 0.4 up to >0.6) most frequently were registered in HV platelet during activation by 1 μM TRAP and in SA patients during platelet activation by 20 μM and 5 μM ADP. However, in many other cases these correlations were rather weak (r < 0.3) and sometimes statistically insignificant. In HV the differences in PAC-1 binding parameters between platelets activated by 10 μM TRAP (the strongest agonist) and all ADP concentrations were negligible (≤ 10%), while CD62P binding (at all ADP concentrations) and LTA parameters for (5 μM and 2.5 μM ADP) were significantly lower (by 40-60%). Antiplatelet therapy in patients decreased all parameters as compared to HV, but to varying extents. For 10 μM TRAP the MFI index for PAC-1 binding (40-50% decrease) and for both ADP concentrations the Tmax values (60-85% decrease) appeared to be the most sensitive in comparison with the other parameters that decreased to a lesser extent. The data obtained indicate a possibility of inconsistency between different LTA and flow cytometry parameters in assessing platelet activity and efficacy of antiplatelet drugs.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"70 2","pages":"99-108"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative proteomic analysis of renal tissue of normotensive and hypertensive rats. 正常血压大鼠和高血压大鼠肾组织的比较蛋白质组分析

Biomeditsinskaya khimiya Pub Date : 2024-04-01 DOI: 10.18097/PBMC20247002089

O A Buneeva, V I Fedchenko, S A Kaloshina, M G Zavyalova, V G Zgoda, A E Medvedev

{"title":"Comparative proteomic analysis of renal tissue of normotensive and hypertensive rats.","authors":"O A Buneeva, V I Fedchenko, S A Kaloshina, M G Zavyalova, V G Zgoda, A E Medvedev","doi":"10.18097/PBMC20247002089","DOIUrl":"https://doi.org/10.18097/PBMC20247002089","url":null,"abstract":"Comparative proteomic analysis of kidney tissue from normotensive (WKY) and spontaneously hypertensive (SHR) rats revealed quantitative and qualitative changes in renal proteins. The number of renal proteins specific for WKY rats (blood pressure 110-120 mm Hg) was 13-16. There were 20-24 renal proteins specific for SHR (blood pressure 180 mm Hg and more). The total number of identified renal proteins common for both rat strains included 972-975 proteins. A pairwise comparison of all possible (SHR-WKY) variants identified 8 proteins specific only for normotensive (WKY) animals, and 7 proteins specific only for hypertensive ones (SHR). Taking into consideration their biological roles, the lack of some enzyme proteins in hypertensive rats (for example, biliverdin reductase A) reduces the production of molecules exhibiting antihypertensive properties, while the appearance of others (e.g. betaine-homocysteine S-methyltransferase 2, septin 2, etc.) can be interpreted as a compensatory reaction. Renal proteins with altered relative content (with more than 2.5-fold change) accounted for no more than 5% of all identified proteins. Among the proteins with an increased relative content in hypertensive animals, the largest group consisted of proteins involved in the processes of energy generation and carbohydrate metabolism, as well as antioxidant and protective proteins. In the context of the development of hypertension, the identified relative changes can apparently be considered compensatory. Among the proteins with the most pronounced decrease in the relative content in hypertensive rats, the dramatic reduction in acyl-CoA medium-chain synthetase-3 (ACSM3) appears to make an important contribution to the development of renal pathology in these animals.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"70 2","pages":"89-98"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of the content of reactive oxygen species and the state of the glutathione system in the oral cavity during subchronic intoxication wuth the fungicide thiram and its antioxidant correction. 杀菌剂福双美的亚慢性中毒及其抗氧化剂校正过程中口腔中活性氧含量和谷胱甘肽系统状态的动态变化。

Biomeditsinskaya khimiya Pub Date : 2024-04-01 DOI: 10.18097/PBMC20247002073

V A Korolev, E V Felker, L A Yachmeneva, L A Babkina, Y A Azarova, M I Churilin, A I Milova

{"title":"Dynamics of the content of reactive oxygen species and the state of the glutathione system in the oral cavity during subchronic intoxication wuth the fungicide thiram and its antioxidant correction.","authors":"V A Korolev, E V Felker, L A Yachmeneva, L A Babkina, Y A Azarova, M I Churilin, A I Milova","doi":"10.18097/PBMC20247002073","DOIUrl":"https://doi.org/10.18097/PBMC20247002073","url":null,"abstract":"Thiram is a dithiocarbamate derivative, which is used as a fungicide for seed dressing and spraying during the vegetation period of plants, and also as an active vulcanization accelerator in the production of rubber-based rubber products. In this study the content of reactive oxygen species (ROS) and the state of the glutathione system have been investigated in the oral fluid and gum tissues of adult male Wistar rats treated with thiram for 28 days during its administration with food at a dose of 1/50 LD50. Thiram induced formation of ROS in the oral cavity; this was accompanied by an imbalance in the ratio of reduced and oxidized forms of glutathione due to a decrease in glutathione and an increase in its oxidized form as compared to the control. Thiram administration caused an increase in the activity of glutathione-dependent enzymes (glutathione peroxidase, glutathione transferase, and glutathione reductase). However, the time-course of enzyme activation in the gum tissues and oral fluid varied in dependence on the time of exposure to thiram. In the oral fluid of thiram-treated rats changes in the antioxidant glutathione system appeared earlier. The standard diet did not allow the glutathione pool to be fully restored to physiological levels after cessation of thiram intake. The use of exogenous antioxidants resviratrol and an Echinacea purpurea extract led to the restoration of redox homeostasis in the oral cavity.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"70 2","pages":"73-82"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variability of haptoglobin beta-chain proteoforms. 血红蛋白β链蛋白形式的变异性。

Biomeditsinskaya khimiya Pub Date : 2024-04-01 DOI: 10.18097/PBMC20247002114

N L Ronzhina, E S Zorina, M G Zavialova, O K Legina, S N Naryzhny

{"title":"Variability of haptoglobin beta-chain proteoforms.","authors":"N L Ronzhina, E S Zorina, M G Zavialova, O K Legina, S N Naryzhny","doi":"10.18097/PBMC20247002114","DOIUrl":"10.18097/PBMC20247002114","url":null,"abstract":"Existing knowledge on changes of the haptoglobin (Hp) molecule suggests that it may exist in multiple proteoforms, which obviously exhibit different functions. Using two-dimensional electrophoresis (2DE) in combination with mass spectrometry and immunodetection, we have analyzed blood plasma samples from both healthy donors and patients with primary grade IV glioblastoma (GBM), and obtained a detailed composite 2DE distribution map of β-chain proteoforms, as well as the full-length form of Hp (zonulin). Although the total level of plasma Hp exceeded normal values in cancer patients (especially patients with GBM), the presence of particuar proteoforms, detected by their position on the 2DE map, was very individual. Variability was found in both zonulin and the Hp β-chain. The presence of an alkaline form of zonulin in plasma can be considered a conditional, but insufficient, GBM biomarker. In other words, we found that at the level of minor proteoforms of Hp, even in normal conditions, there was a high individual variability. On the one hand, this raises questions about the reasons for such variability, if it is present not only in Hp, but also in other proteins. On the other hand, this may explain the discrepancy between the number of experimentally detected proteoforms and the theoretically possible ones not only in Hp, but also in other proteins.","PeriodicalId":8889,"journal":{"name":"Biomeditsinskaya khimiya","volume":"70 2","pages":"114-124"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140848546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0