Bioengineering最新文献

{"title":"Evaluation of Phosphene Shifts During Eye Movements to Enhance Safe Visual Assistance for Visually Impaired Individuals.","authors":"Manami Kanamaru, Keita Tanaka, Eiji Kamioka","doi":"10.3390/bioengineering12030281","DOIUrl":"10.3390/bioengineering12030281","url":null,"abstract":"<p><p>Hands-free visual assistive devices that consider the safety of the visually impaired have been researched, but many of them interfere with other senses, such as hearing. Therefore, phosphenes have been researched as a method of presenting visual information. Phosphenes are flashes that are recognized by electrical stimulation, and the presentation position can be adjusted by the electrode arrangement. However, it has been reported that the presentation position changes significantly when the eyeballs are moved as far left and right as possible. As a walking assistive device for the visually impaired, the fluctuation of the presentation position of phosphenes may cause safety problems. This study used the Hess test to verify the positional fluctuation of phosphenes associated with eye movement and compared it with eye movement during walking to discuss safety. As a result, the range of the change in the presentation position of phosphenes was significantly large when subjects moved their eyes to the peripheral vision, the same as in the previous study. On the other hand, the presentation position of phosphenes did not change significantly within the range of eye movement during walking (±15 deg). Our results suggest that there is the possibility that serious safety issues will not happen with assistive devices for the visually impaired who use phosphenes.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-Time Integration of Optical Coherence Tomography Thickness Map Overlays for Enhanced Visualization in Epiretinal Membrane Surgery: A Pilot Study.","authors":"Ferhat Turgut, Keisuke Ueda, Amr Saad, Tahm Spitznagel, Luca von Felten, Takashi Matsumoto, Rui Santos, Marc D de Smet, Zoltán Zsolt Nagy, Matthias D Becker, Gábor Márk Somfai","doi":"10.3390/bioengineering12030271","DOIUrl":"10.3390/bioengineering12030271","url":null,"abstract":"<p><p>(1) Background: The process of epiretinal membrane peeling (MP) requires precise intraoperative visualization to achieve optimal surgical outcomes. This study investigates the integration of preoperative Optical Coherence Tomography (OCT) images into real-time surgical video feeds, providing a dynamic overlay that enhances the decision-making process during surgery. (2) Methods: Five MP surgeries were analyzed, where preoperative OCT images were first manually aligned with the initial frame of the surgical video by selecting five pairs of corresponding points. A homography transformation was then computed to overlay the OCT onto that first frame. Subsequently, for consecutive frames, feature point extraction (the Shi-Tomasi method) and optical flow computation (the Lucas-Kanade algorithm) were used to calculate frame-by-frame transformations, which were applied to the OCT image to maintain alignment in near real time. (3) Results: The method achieved a 92.7% success rate in optical flow detection and maintained an average processing speed of 7.56 frames per second (FPS), demonstrating the feasibility of near real-time application. (4) Conclusions: The developed approach facilitates enhanced intraoperative visualization, providing surgeons with easier retinal structure identification which results in more comprehensive data-driven decisions. By improving surgical precision while potentially reducing complications, this technique benefits both surgeons and patients. Furthermore, the integration of OCT overlays holds promise for advancing robot-assisted surgery and surgical training protocols. This pilot study establishes the feasibility of real-time OCT integration in MP and opens avenues for broader applications in vitreoretinal procedures.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Compression Testing Methods for Murine Lumbar Vertebral Bodies: Identifying Most Reliable and Reproducible Techniques for Assessing Compressive Strength.","authors":"Daniel Kronenberg, Britta Wieskoetter, Sarah Soeger, Heriburg Hidding, Melanie Timmen, Michael J Raschke, Richard Stange","doi":"10.3390/bioengineering12030273","DOIUrl":"10.3390/bioengineering12030273","url":null,"abstract":"<p><p>This study evaluates four compression testing methods to determine the most reliable and reproducible technique for assessing the compression strength of murine lumbar vertebral bodies. Twenty female C57BL/6 mice (12 weeks old) were randomized into four groups: Group 1, compression of the complete lumbar vertebral body (LVB) with dorsal spinal processes; Group 2, compression at the vertebral body surface; Group 3, compression at the vertebral body surface after vertebral arch resection; Group 4, resection of the vertebral arch with straightening of the intervertebral joint surface. A mono-axial static testing machine applied compression, measuring load to failure, stiffness, yield load, and elasticity modulus. Method 1 resulted in significantly higher load-to-failure and yield-to-failure (25.9 N compared to 18.2 N, and twice 12 N for Methods 2-4), with the least variation in relative values. Method 3 had increased stiffness and a significantly higher Young's modulus (232 N/mm, in contrast to 101, 130, and 145 N/mm for Methods 1, 2, and 4, respectively) but yielded inconsistent results. Method 4 showed the greatest variability across specimens. Method 2 yields suitable data quality as well, albeit with a slightly higher variation, and is the recommended procedure if the spinal processes have to be excluded from the measurement. Based on these findings, Method 1 produced the most consistent and reproducible data and is recommended for future studies evaluating vertebral biomechanics in mice.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing Growth Models Dependent on Irradiation and Nutrient Consumption on Closed Outdoor Cultivations of <i>Nannochloropsis</i> sp.","authors":"Tiago Taborda, José C M Pires, Sara M Badenes, Francisco Lemos","doi":"10.3390/bioengineering12030272","DOIUrl":"10.3390/bioengineering12030272","url":null,"abstract":"<p><p>Microalgae offer tremendous industrial possibilities for their ability to grow rapidly and capture CO₂ from the atmosphere. The literature contains many models for predicting microalgae growth in lab-scale reactors. However, there exists a gap in the application of these models in outdoor pilot-scale closed photobioreactors. This work proposes a methodology for constructing models for this type of reactor. These models were constructed based on the existing literature, then trained and tested using a dataset of ten cultivations of <i>Nannochloropsis</i> sp. Four models were tested: a model based on a Monod-like equation (Model M); a model based on a Haldane-like equation (Model H); a model based on an exponential equation (Model E); and a model considering both irradiation and the effect of nitrate on the culture using the Droop model (Model D). Model H had the best overall performance, with a global root mean squared error (RMSE) of 0.296 kg^1/2 m^-3/2; Model M and Model E had RMSE values of 0.309 and 0.302, respectively. Model D performed the worst, with an RMSE of 0.413. Future work should involve applying the same methodology to new cultivations of the same or different species and testing more complex models capable of better explaining the data.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Printing of Hydrogel as Skin Substitute and Comparative Evaluation of Melanin Production.","authors":"Mohammad Zafaryab, Komal Vig","doi":"10.3390/bioengineering12030270","DOIUrl":"10.3390/bioengineering12030270","url":null,"abstract":"<p><p>Cell culture in two dimensions has been the main instrument in cellular and molecular biology. But there are limitations to two-dimensional culture when it comes to tissue engineering and in vivo reproduction. Tissue engineering technology enabled the creation of biomedical scaffolds, which are mostly utilized to biofabricate different artificial human organs. Tissue architecture that encourage cell proliferation can be produced using direct bioprinting technology. The development of bioinks for 3D bioprinting is consistently seen as a problem in the domains of biofabrication and tissue engineering. This study aimed to determine if Fibroblasts and Keratinocytes could grow on hydrogel scaffolds as efficiently as they can in the culture plates. Melanocytes were co-cultured, and the production of melanin was assessed in a two- and three-dimensional culture system. Scaffolds were fabricated using 8% alginate and 6% gelatin and 3D-printed using a cell link printer. FTIR was used to determine the precise composition of the gels. SEM analysis was performed for the cells present in gel and the topology of the cells. In addition, 8% alginate and 6% alginate gel scaffolds were analyzed for swelling and degradation over time in the cell growth medium and PBS. Furthermore, a gene expression study of cell cultures on scaffolds was performed through qPCR. A live/dead assay was performed to determine cell viability for cells grown on scaffolds for 7, 14, and 21 days. Most of the cells were shown to be viable, similar to the control cells grown on a plate. The findings from the SEM showed that cells were grown on the gel surface, remained viable even after 21 days, and displayed circular cells stacked three-dimensionally on the gel surface in the 3D scaffold. The MTT assay was performed to check the viability of cells cultured on a 3D-printed scaffold for 1, 5, and 15 days. We observed about 40% viable cells after 15 days, as shown by the MTT assay. Furthermore, a co-culture study with Melanocyte showed an increased production of melanin in a 3D culture as compared to a 2D culture. Our findings suggest that an alginate and gelatin polymer can be used as a cellular matrix for epithelial cell culture. Further, in vivo and ex vivo experiments are needed to validate the results for future applications in tissue engineering for wound healing and other tissue engineering domains.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Machine Learning Algorithms-Enhanced Predication for Cervical Cancer from Mass Spectrometry-Based Proteomics Data.","authors":"Da Zhang, Lihong Zhao, Bo Guo, Aihong Guo, Jiangbo Ding, Dongdong Tong, Bingju Wang, Zhangjian Zhou","doi":"10.3390/bioengineering12030269","DOIUrl":"10.3390/bioengineering12030269","url":null,"abstract":"<p><p>Early diagnosis is critical for improving outcomes in cancer patients; however, the application of diagnostic markers derived from serum proteomic screening remains challenging. Artificial intelligence (AI), encompassing deep learning and machine learning (ML), has gained increasing prominence across various scientific disciplines. In this study, we utilized cervical cancer (CC) as a model to develop an AI-driven pipeline for the identification and validation of serum biomarkers for early cancer diagnosis, leveraging mass spectrometry-based proteomics data. By processing and normalizing serum polypeptide differential peaks from 240 patients, we employed eight distinct ML algorithms to classify and analyze these differential polypeptide peaks, subsequently constructing receiver operating characteristic (ROC) curves and confusion matrices. Key performance metrics, including accuracy, precision, recall, and F1 score, were systematically evaluated. Furthermore, by integrating feature importance values, Shapley values, and local interpretable model-agnostic explanation (LIME) values, we demonstrated that the diagnostic area under the curve (AUC) achieved by our multi-dimensional learning models approached 1, significantly outperforming the diagnostic AUC of single markers derived from the PRIDE database. These findings underscore the potential of proteomics-driven integrated machine learning as a robust strategy to enhance early cancer diagnosis, offering a promising avenue for clinical translation.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison of Different Biomechanical Systems for the Orthodontic Treatment of Palatally Impacted Canines.","authors":"Ioannis P Zogakis, Chrysanthi Anagnostou, Ioulia Ioannidou, Stella Chaushu, Moschos A Papadopoulos","doi":"10.3390/bioengineering12030267","DOIUrl":"10.3390/bioengineering12030267","url":null,"abstract":"<p><p>Canine impaction constitutes a clinical entity that, if untreated, can compromise dentition. Thus, various treatment approaches and different biomechanical systems have been proposed over the years for its management. Clinical records of patients who consecutively visited the Postgraduate Clinic of the Department of Orthodontics of the Aristotle University of Thessaloniki, Greece, were retrieved and analyzed retrospectively with the aim to compare two different biomechanical systems for the orthodontic/surgical treatment of palatally displaced canines. A total of 29 patients with 36 palatally impacted canines were included in the current investigation. The patients had a median age of 16 years (IQR: 15-20); 69% of them were females (n = 20) and 31% were male (n = 9). No statistically significant differences regarding treatment outcomes were detected between different types of active unit (<i>p</i> > 0.99), or the types of bonded attachments (<i>p</i> = 0.52). The use of ballista springs or cantilever configurations was not found to significantly affect the alignment duration (<i>p</i> = 0.56) as opposed to the type of attachment, where eyelets outmatch brackets (<i>p =</i> 0.009). The use of brackets over eyelets significantly prolongs the canine alignment duration.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Ion Channels: Blockers Suppress Calcium Signals and Induce Cytotoxicity Across Medulloblastoma Cell Models.","authors":"Darani Ashley Thammavongsa, Taylor N Jackvony, Markus J Bookland, Min D Tang-Schomer","doi":"10.3390/bioengineering12030268","DOIUrl":"10.3390/bioengineering12030268","url":null,"abstract":"<p><p>Medulloblastoma (MB) groups 3 and 4 lack targeted therapies despite their dismal prognoses. Ion channels and pumps have been implicated in promoting MB metastasis and growth; however, their roles remain poorly understood. In this study, we repurposed FDA-approved channel blockers and modulators to investigate their potential anti-tumor effects in MB cell lines (DAOY and D283) and primary cell cultures derived from a patient with MB. For the first time, we report spontaneous calcium signaling in MB cells. Spontaneous calcium signals were significantly reduced by mibefradil (calcium channel blocker), paxilline (calcium-activated potassium channel blocker), and thioridazine (potassium channel blocker). These drugs induced dose-dependent cytotoxicity in both the DAOY and D283 cell lines, as well as in primary cell cultures of a patient with group 3 or 4 MB. In contrast, digoxin and ouabain, inhibitors of the Na/K pump, reduced the calcium signaling by over 90% in DAOY cells and induced approximately 90% cell death in DAOY cells and 80% cell death in D283 cells. However, these effects were significantly diminished in the cells derived from a patient with MB, highlighting the variability in drug sensitivity among MB models. These findings demonstrate that calcium signaling is critical for MB cell survival and that the targeted inhibition of calcium pathways suppresses tumor cell growth across multiple MB models.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extending the Three-Dimensional Culture of Adipocytes Through Surface Coatings.","authors":"Sheetal Chowdhury, Komal Beeton, Zacchaeus Wallace, Maggie Moore, Gene L Bidwell, Amol V Janorkar","doi":"10.3390/bioengineering12030266","DOIUrl":"10.3390/bioengineering12030266","url":null,"abstract":"<p><p>To mimic the important features of progressing adiposity, in vitro adipose cell culture models must allow gradual intracellular fat accumulation in the three-dimensional (3D) arrangement of adipose-derived stem cells (ASCs) over a long-term culture period. Previously, elastin-like polypeptide (ELP) and polyethyleneimine (PEI) have been used to culture human adipose-derived stem cells (hASCs) as 3D spheroids and to differentiate them to adipocytes over a relatively long culture period of up to 5 weeks. In this study, to further enhance the spheroid adhesion properties, ELP was fused with Arginine-Glycine-Aspartic Acid (RGD) residues, known for their role as cell-attachment sites. This study aimed to assess whether the addition of RGD to the C-or N-terminus of ELP would impact the spheroid-forming ability of ELP-PEI coatings. ELP-RGD conjugates were produced using genetically modified <i>Escherichia coli</i> to express ELP-(RGD)₃ and (RGD)₃-ELP, followed by chemical conjugation with PEI. SDS gel electrophoresis, FTIR spectroscopy, and turbidimetry analyses revealed that ELP was conjugated with RGD without much alteration in the molecular weight, functional groups present, and transition temperature of ELP. The addition of RGD to ELP also did not affect the chemical conjugation capacity of ELP to PEI. We observed that the ELP-PEI coating formed slightly larger spheroids (61.8 ± 3.2 µm) compared to the ELP-(RGD)₃-PEI and (RGD)₃-ELP-PEI coatings (56.6 ± 3.0 and 53.4 ± 2.4 µm, respectively). Despite the size difference, ELP-(RGD)₃-PEI coatings exhibited superior spheroid retention during media changes, with minimal spheroid loss. DNA assay results confirmed a significant decrease in the DNA concentration (<i>p</i> < 0.05) after the 20 media changes for spheroids cultured on the ELP-PEI coating, indicating spheroid loss. However, there was no significant difference in DNA concentration before and after 20 media changes for spheroids cultured on the ELP-(RGD)₃-PEI and (RGD)₃-ELP-PEI coatings (<i>p</i> > 0.05). These findings suggest that RGD incorporation does not hinder the initial spheroid formation ability of the ELP-PEI coating and enhances spheroid retention under dynamic culture conditions.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Combination of PPARα Agonist GW7647 and Imeglimin Has Potent Effects on High-Glucose-Induced Cellular Biological Responses in Human Retinal Pigment Epithelium Cells.","authors":"Nami Nishikiori, Megumi Watanabe, Megumi Higashide, Araya Umetsu, Toshifumi Ogawa, Masato Furuhashi, Hiroshi Ohguro, Tatsuya Sato","doi":"10.3390/bioengineering12030265","DOIUrl":"10.3390/bioengineering12030265","url":null,"abstract":"<p><strong>Background: </strong>Hyperglycemic changes in the cellular biological properties of retinal pigment epithelium cells are involved in the pathophysiology of diabetic retinopathy (DR). To assess the effects of the new anti-diabetic agent imeglimin (Ime) on DR, the pharmacological effects of Ime and those of metformin (Met) in combination with the PPARα agonist GW7646 (GW) on adult retinal pigment epithelium (ARPE19) cells cultured in high-glucose conditions were compared.</p><p><strong>Methods: </strong>Cell viability, levels of reactive oxygen species (ROS), monolayer barrier function measured by transepit very much helial electrical resistance (TEER), and metabolic functions determined by an extracellular flux analyzer were evaluated.</p><p><strong>Results: </strong>While glucose concentrations did not alter cell viability regardless of the presence of Met or Ime, levels of ROS were significantly increased by the high-glucose conditions, and increased levels of ROS were significantly alleviated by the combination of Ime and GW but not by Met alone. Similarly, TEER values were increased by high-glucose conditions, but the effects of high-glucose conditions were dramatically enhanced by the combination of Ime and GW. Furthermore, a metabolic assay showed that an energetic shift was induced by the combination of Ime and GW, whereas energy status became quiescent with Met or Ime alone.</p><p><strong>Conclusions: </strong>The collective results suggest that Ime in combination with GW has synergetic effects on high-glucose-induced cellular biological changes in ARPE19 cells.</p>","PeriodicalId":8874,"journal":{"name":"Bioengineering","volume":"12 3","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}