Bioscience of Microbiota, Food and Health最新文献_第7页

Creatine supplementation enhances immunological function of neutrophils by increasing cellular adenosine triphosphate. 补充肌酸通过增加细胞三磷酸腺苷来增强中性粒细胞的免疫功能。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2022-01-01 Epub Date: 2022-06-17 DOI: 10.12938/bmfh.2022-018

Suguru Saito, Duo-Yao Cao, Alato Okuno, Xiaomo Li, Zhenzi Peng, Musin Kelel, Noriko M Tsuji

{"title":"Creatine supplementation enhances immunological function of neutrophils by increasing cellular adenosine triphosphate.","authors":"Suguru Saito, Duo-Yao Cao, Alato Okuno, Xiaomo Li, Zhenzi Peng, Musin Kelel, Noriko M Tsuji","doi":"10.12938/bmfh.2022-018","DOIUrl":"https://doi.org/10.12938/bmfh.2022-018","url":null,"abstract":"Creatine is an organic compound which is utilized in biological activities, especially for adenosine triphosphate (ATP) production in the phosphocreatine system. This is a well-known biochemical reaction that is generally recognized as being mainly driven in specific parts of the body, such as the skeletal muscle and brain. However, our report shows a novel aspect of creatine utilization and ATP synthesis in innate immune cells. Creatine supplementation enhanced immune responses in neutrophils, such as cytokine production, reactive oxygen species (ROS) production, phagocytosis, and NETosis, which were characterized as antibacterial activities. This creatine-induced functional upregulation of neutrophils provided a protective effect in a murine bacterial sepsis model. The mortality rate in mice challenged with Escherichia coli K-12 was decreased by creatine supplementation compared with the control treatment. Corresponding to this decrease in mortality, we found that creatine supplementation decreased blood pro-inflammatory cytokine levels and bacterial colonization in organs. Creatine supplementation significantly increased the cellular ATP level in neutrophils compared with the control treatment. This ATP increase was due to the phosphocreatine system in the creatine-treated neutrophils. In addition, extracellular creatine was used in this ATP synthesis, as inhibition of creatine uptake abolished the increase in ATP in the creatine-treated neutrophils. Thus, creatine is an effective nutrient for modifying the immunological function of neutrophils, which contributes to enhancement of antibacterial immunity.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5e/ef/bmfh-41-185.PMC9533032.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40337652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Average gut flora in healthy Japanese subjects stratified by age and body mass index 日本健康受试者的平均肠道菌群按年龄和体重指数分层

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2021-12-07 DOI: 10.12938/bmfh.2021-056

N. Yoshida, S. Watanabe, Hiroyuki Yamasaki, Hajime Sakuma, Aya K Takeda, T. Yamashita, K. Hirata

{"title":"Average gut flora in healthy Japanese subjects stratified by age and body mass index","authors":"N. Yoshida, S. Watanabe, Hiroyuki Yamasaki, Hajime Sakuma, Aya K Takeda, T. Yamashita, K. Hirata","doi":"10.12938/bmfh.2021-056","DOIUrl":"https://doi.org/10.12938/bmfh.2021-056","url":null,"abstract":"Imbalance of the gut microbiota plays an important role in the pathogenesis of various diseases. Although many clinical studies have analyzed the gut microbiota, the definition of normal gut microbiota remains unclear. In this study, we aim to establish the average gut microbiota in the healthy Japanese population. Using 16S ribosomal RNA gene sequencing, we analyzed gut microbial data from fecal samples obtained from 6,101 healthy Japanese individuals. Based on their ages, the individuals were divided into three groups: young, middle-age, and old. Individuals were further categorized according to body mass index (BMI) into lean, normal, and obese groups. The α and β diversities in the old group were significantly higher than those in the young and middle-age groups. The Firmicutes/Bacteroidetes ratio of subjects in the obese category was significantly lower compared with those of subjects in the lean and normal categories in the young and middle-age groups. Genus Bacteroides was the dominant gut microbiota across all the BMI categories in all the age groups. Among the top ten genera, the abundances of Bacteroides, Bifidobacterium, Anaerostipes, Blautia, Dorea, Fusicatenibacter, Lachnoclostridium, and Parabacteroides were significantly lower in the old group than in the young and middle-age groups. The correlation network at the genus level revealed different microbe-microbe interactions associated with age and BMI. We determined the average Japanese gut microbiota, and this information could be used as a reference. The gut microbiota greatly differs based on the life stage and metabolic status of the host, and this gives rise to a variety of host–gut microbe interactions that can lead to an increased susceptibility to disease.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2021-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85355634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Clostridium butyricum therapy for mild-moderate Clostridioides difficile infection and the impact of diabetes mellitus 丁酸梭菌治疗轻、中度艰难梭菌感染及对糖尿病的影响

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2021-11-03 DOI: 10.12938/bmfh.2021-049

Jen-Chieh Lee, Chun-Wei Chiu, P. Tsai, Ching-Chi Lee, I. Huang, W. Ko, Y. Hung

{"title":"Clostridium butyricum therapy for mild-moderate Clostridioides difficile infection and the impact of diabetes mellitus","authors":"Jen-Chieh Lee, Chun-Wei Chiu, P. Tsai, Ching-Chi Lee, I. Huang, W. Ko, Y. Hung","doi":"10.12938/bmfh.2021-049","DOIUrl":"https://doi.org/10.12938/bmfh.2021-049","url":null,"abstract":"The therapeutic effect of Clostridium butyricum for adults with Clostridioides difficile infection (CDI) was investigated. A retrospective study was conducted in medical wards of Tainan Hospital, Ministry of Health and Welfare, between January 2013 and April 2020. The disease severity of CDI was scored based on the Clinical Practice Guidelines of the IDSA/SHEA. Treatment success was defined as the resolution of diarrhea within six days of a therapeutic intervention without the need to modify the therapeutic regimen. In total, 241 patients developed CDI during hospitalization in the study period. The treatment success rates for the 99 patients with mild-moderate CDI among them were as follows: metronidazole, 69.4%; C. butyricum, 68.2%; metronidazole plus C. butyricum, 66.7%; and oral vancomycin, 66.7% (p=1.00). Patients with treatment success were less likely to have diabetes mellitus than those with treatment failure (38.2% vs. 61.3%, p=0.05). Patients treated with C. butyricum alone or in combination with metronidazole had shorter durations of diarrhea than those treated with metronidazole alone (3.1 ± 2.0 days or 3.5 ± 2.4 days vs. 4.2 ± 3.5 days; p=0.43 or 0.71), although the differences were not statistically significant. In conclusion, the treatment success rate of C. butyricum alone or in combination with metronidazole for patients with CDI was non inferior to that of metronidazole alone. The presence of diabetes mellitus in affected individuals is a risk factor for treatment failure.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2021-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82407261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Corrigendum: Alleviation of low-fiber diet-induced constipation by probiotic Bifidobacterium bifidum G9-1 is based on correction of gut microbiota dysbiosis. 更正:益生菌两歧双歧杆菌G9-1缓解低纤维饮食引起的便秘是基于纠正肠道菌群失调。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2021-10-01 DOI: 10.12938/bmfh.18-020c

引用次数: 3

Fecal microbiota transplantation as a new treatment for canine inflammatory bowel disease. 粪便菌群移植作为治疗犬炎症性肠病的新方法。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2020-12-10 DOI: 10.12938/bmfh.2020-049

Ayaka Niina, Ryoko Kibe, Ryohei Suzuki, Yunosuke Yuchi, Takahiro Teshima, Hirotaka Matsumoto, Yasushi Kataoka, Hidekazu Koyama

{"title":"Fecal microbiota transplantation as a new treatment for canine inflammatory bowel disease.","authors":"Ayaka Niina, Ryoko Kibe, Ryohei Suzuki, Yunosuke Yuchi, Takahiro Teshima, Hirotaka Matsumoto, Yasushi Kataoka, Hidekazu Koyama","doi":"10.12938/bmfh.2020-049","DOIUrl":"https://doi.org/10.12938/bmfh.2020-049","url":null,"abstract":"In human medicine, fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridioides difficile infection. It has also been tested as a treatment for multiple gastrointestinal diseases, including inflammatory bowel disease (IBD). However, only a few studies have focused on the changes in the microbiome following FMT for canine IBD. Here, we performed FMT in nine dogs with IBD using the fecal matter of healthy dogs and investigated the subsequent changes in the fecal microbiome and clinical signs. In three dogs, the fecal microbiome was examined by 16S rRNA sequencing. Fusobacteria were observed at a low proportion in dogs with IBD. However, the post-FMT microbiome became diverse and showed a significant increase in Fusobacteria proportion. Fusobacterium was detected in the nine dogs by quantitative polymerase chain reaction. The proportion of Fusobacterium in the post-FMT fecal microbiome was significantly increased (p<0.05). The changes in clinical signs (e.g., vomiting, diarrhea, and weight loss) were evaluated according to the canine inflammatory bowel disease activity index. The score of this index significantly decreased in all dogs (p<0.05) with improvements in clinical signs. These improvements were related to the changes in the proportion of microbes, particularly the increase in Fusobacterium. The dogs with IBD showed a lower proportion of Fusobacterium than healthy dogs. This suggests that a low proportion of Fusobacterium is a characteristic feature of canine IBD and that Fusobacterium is involved in this disease. The results of this study may help elucidate the pathogenesis of this disease and its association with Fusobacterium.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/10/bc/bmfh-40-098.PMC8099633.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38906255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

The importance of genetic research on the dominant species of human intestinal indigenous microbiota. 人类肠道原生微生物群优势种遗传学研究的重要性。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2020-09-10 DOI: 10.12938/bmfh.2020-011

Shin Kurihara

{"title":"The importance of genetic research on the dominant species of human intestinal indigenous microbiota.","authors":"Shin Kurihara","doi":"10.12938/bmfh.2020-011","DOIUrl":"https://doi.org/10.12938/bmfh.2020-011","url":null,"abstract":"Comparisons of the changes in the gut microbiota and transcriptomes as a result of changes in diet have demonstrated that the regulation of the gene functions of intestinal bacteria is fundamental for the regulation of the intestinal environment. However, the functions of only about half of the genes can be predicted using nucleotide sequences obtained from the metagenomic data of the human gut microbiota. Therefore, the regulation of gut bacterial gene functions is hindered. To resolve this issue, the functions of the genes of intestinal bacteria must be identified. In our previous study, a high-throughput cultivation system was established for the dominant species of indigenous human intestinal microbiota. Using this system, we analyzed the synthesis and transport of polyamines by intestinal bacteria. Comparison of the results with those obtained by in silico analysis indicated the existence of novel polyamine synthetic enzymes and transport proteins. Next, strains with gene deletions and complementation for the polyamine synthetic system of the genus Bacteroides were analyzed. Furthermore, we co-cultured genetically engineered Escherichia coli and Enterococcus faecalis strains to demonstrate the presence of a polyamine synthetic pathway spanning multiple bacterial species. Here, we outline the trends of research using genetically engineered intestinal bacteria and the ripple effects of studies in which intestinal bacteria have been analyzed genetically. Moreover, because studies on intestinal bacteria at the gene level are indispensable for improving our understanding of their regulation, the importance of this research will continue to increase in the future.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25315816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Heat-killed Lactobacillus plantarum L-137 attenuates obesity and associated metabolic abnormalities in C57BL/6 J mice on a high-fat diet. 热灭活植物乳杆菌L-137减轻高脂饮食C57BL/6 J小鼠的肥胖和相关代谢异常。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2020-11-14 DOI: 10.12938/bmfh.2020-040

Rieko Yoshitake, Yoshitaka Hirose, Shinji Murosaki, Goro Matsuzaki

{"title":"Heat-killed Lactobacillus plantarum L-137 attenuates obesity and associated metabolic abnormalities in C57BL/6 J mice on a high-fat diet.","authors":"Rieko Yoshitake, Yoshitaka Hirose, Shinji Murosaki, Goro Matsuzaki","doi":"10.12938/bmfh.2020-040","DOIUrl":"https://doi.org/10.12938/bmfh.2020-040","url":null,"abstract":"Heat-killed Lactobacillus plantarum L-137 (HK L-137) has anti-allergic, antitumor, and antiviral effects in mice, as well as an anti-inflammatory effect in rats with metabolic syndrome through regulation of immunity. To evaluate the influence of HK L-137 on chronic inflammation in mice with diet-induced obesity, C57BL/6 J mice were fed a normal diet (16% of energy as fat) or a high-fat diet (62% of energy as fat) with or without 0.002% HK L-137 for 4 to 20 weeks. It was found that HK L-137 supplementation alleviated weight gain and elevation of plasma glucose, cholesterol, alanine aminotransferase, and aspartate transaminase levels in mice with diet-induced obesity. Expression of several inflammation-related genes, including F4/80, CD11c, and IL-1β, in the epididymal adipose tissue of these mice was significantly downregulated by HK L-137. In addition, plasma levels of lipopolysaccharide-binding protein, a marker of endotoxemia, tended to be decreased by administration of HK L-137. These findings suggest that HK L-137 supplementation ameliorates obesity-induced metabolic abnormalities and adipose tissue inflammation, possibly through improvement of intestinal permeability.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/20/c8/bmfh-40-084.PMC8099634.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38906253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Characterisation of gut microbiota of obesity and type 2 diabetes in a rodent model. 在啮齿动物模型中肥胖症和2型糖尿病的肠道微生物群特征。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2020-10-10 DOI: 10.12938/bmfh.2019-031

Khalid S Ibrahim, Nowara Bourwis, Sharron Dolan, Sue Lang, Janice Spencer, John A Craft

{"title":"Characterisation of gut microbiota of obesity and type 2 diabetes in a rodent model.","authors":"Khalid S Ibrahim, Nowara Bourwis, Sharron Dolan, Sue Lang, Janice Spencer, John A Craft","doi":"10.12938/bmfh.2019-031","DOIUrl":"https://doi.org/10.12938/bmfh.2019-031","url":null,"abstract":"Various studies have suggested that the gut microbiome interacts with the host and may have a significant role in the aetiology of obesity and Type 2 Diabetes (T2D). It was hypothesised that bacterial communities in obesity and T2D differ from control and compromise normal interactions between host and microbiota. Obesity and T2D were developed in rats by feeding a high-fat diet or a high-fat diet plus a single low-dose streptozotocin administration, respectively. The microbiome profiles and their metabolic potentials were established by metagenomic 16S rRNA sequencing and bioinformatics. Taxonomy and predicted metabolism-related genes in obesity and T2D were markedly different from controls and indeed from each other. Diversity was reduced in T2D but not in Obese rats. Factors likely to compromise host intestinal, barrier integrity were found in Obese and T2D rats including predicted, decreased bacterial butyrate production. Capacity to increase energy extraction via ABC-transporters and carbohydrate metabolism were enhanced in Obese and T2D rats. T2D was characterized by increased proinflammatory molecules. While obesity and T2D show distinct differences, results suggest that in both conditions Bacteroides and Blautia species were increased indicating a possible mechanistic link.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/35/bb/bmfh-40-065.PMC7817511.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25316306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Analysis of appendectomy samples identified dysbiosis in acute appendicitis. 急性阑尾炎阑尾切除标本的分析鉴定出生态失调。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2020-11-14 DOI: 10.12938/bmfh.2020-051

Shinya Munakata, Mari Tohya, Hirokazu Matsuzawa, Yuki Tsuchiya, Kota Amemiya, Toshiaki Hagiwara, Daisuke Motooka, Shota Nakamura, Kazuhiro Sakamoto, Shin Watanabe

{"title":"Analysis of appendectomy samples identified dysbiosis in acute appendicitis.","authors":"Shinya Munakata, Mari Tohya, Hirokazu Matsuzawa, Yuki Tsuchiya, Kota Amemiya, Toshiaki Hagiwara, Daisuke Motooka, Shota Nakamura, Kazuhiro Sakamoto, Shin Watanabe","doi":"10.12938/bmfh.2020-051","DOIUrl":"https://doi.org/10.12938/bmfh.2020-051","url":null,"abstract":"Appendicitis is the most common cause of sudden-onset abdominal pain requiring surgery. Culture-independent techniques have revealed that the complex intestinal bacterial ecology is associated with various diseases. To evaluate differences in patient characteristics and gut microbiota distribution in patients with appendicitis, we enrolled 12 patients who underwent appendectomy for appendicitis (appendicitis group) and 13 patients who underwent ileocecal resection or right hemicolectomy for colon cancer (control group). Microbiota were analyzed using next-generation sequencing of surgical specimens from appendix swab samples collected postoperatively. Overall differences in the structure of the gut microbiota were evaluated using the α- and β-diversity indices, which were calculated using the weighted or unweighted UniFrac distance. Changes in the gut microbial distribution were taxonomically evaluated at the phylum and genus levels. The α-diversity of observed species was significantly different between patients with and without inflammation of the appendix. The appendiceal microbiome of patients with appendicitis exhibited the highest unweighted UniFrac distances. There were no significant differences at the phylum level. Ruminococcus (p=0.02) and f_erysipelotrichaceae_g_clostridium (p=0.005) were increased in the control group compared with the appendicitis group. This pilot study provides the first report of the correlation of the gut microbiota with the pathogenesis of appendicitis evaluated using mucus-origin sampling.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c7/f7/bmfh-40-092.PMC8099629.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38906254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Analysis of ileostomy stool samples reveals dysbiosis in patients with high-output stomas. 对回肠造口粪便样本的分析显示，高输出量造口患者存在生态失调。

IF 3.1 4区医学

Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2021-02-17 DOI: 10.12938/bmfh.2020-062

Hirokazu Matsuzawa, Shinya Munakata, Masaya Kawai, Kiichi Sugimoto, Hirohiko Kamiyama, Makoto Takahashi, Yutaka Kojima, Kazuhiro Sakamoto

{"title":"Analysis of ileostomy stool samples reveals dysbiosis in patients with high-output stomas.","authors":"Hirokazu Matsuzawa, Shinya Munakata, Masaya Kawai, Kiichi Sugimoto, Hirohiko Kamiyama, Makoto Takahashi, Yutaka Kojima, Kazuhiro Sakamoto","doi":"10.12938/bmfh.2020-062","DOIUrl":"https://doi.org/10.12938/bmfh.2020-062","url":null,"abstract":"Construction of a diverting stoma can significantly reduce the onset of severe anastomotic leakage in patients with rectal cancer. High-output stoma is one of the most important potential surgical complications after anal function-preserving surgery with ileostomy. Culture-independent techniques have revealed the interaction of the complex intestinal bacterial ecology with various diseases. Our objective was to evaluate the differences in patient characteristics and gut microbiota distribution features in patients with high-output stomas. The cases of 24 consecutive patients who underwent curative resection for rectal cancer at our hospital between November 2016 and June 2018 were reviewed, and the patients were categorized into high-output and low-output groups. Their microbiota were analyzed using next-generation sequencing of ileostomy stool samples collected on postoperative day 7. There was a significant difference in the percentage of Bacteroidetes between the high-output and low-output groups (14.8% vs 0.5%; p=0.01). The percentage of Clostridium butyricum was increased in the low-output group (p=0.01). After the exclusion of those treated with the probiotic Miya-BM, whose principal component is C. butyricum, analyses revealed no significant differences between the high-output and low-output groups. This pilot study provides the first evidence correlating gut microbiota with the pathogenesis of high- output stoma compared with low-output stoma.","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/39/a0/bmfh-40-135.PMC8279886.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39203673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4