Bioscience of Microbiota, Food and Health最新文献

{"title":"The composition of the human fecal microbiota might be significantly associated with fecal SCFA levels under hyperbaric conditions.","authors":"Morihiko Oya,&nbsp;Tetsuji Tokunaga,&nbsp;Yutaka Tadano,&nbsp;Hitoshi Ogawa,&nbsp;Shigenori Fujii,&nbsp;Wakana Murakami,&nbsp;Kenji Tamai,&nbsp;Fumitaka Ikomi,&nbsp;Yuji Morimoto","doi":"10.12938/bmfh.2020-054","DOIUrl":"https://doi.org/10.12938/bmfh.2020-054","url":null,"abstract":"<p><p>The fecal microbiota and short-chain fatty acids (SCFAs) play important roles in the human body. This study examined how hyperbaric conditions affect the fecal microbiota and fecal SCFAs. Fecal samples were obtained from 12 divers at three points during deep-diving training (before the diving training, at 2.1 MPa, and after decompression). At 2.1 MPa, the changes in the frequency of <i>Clostridium</i> cluster IV and fecal iso-valerate levels were positively correlated, and the changes in the frequencies of <i>Bacteroides</i> and <i>Clostridium</i> subcluster XIVa were inversely correlated. After decompression, positive correlations were detected between the changes in the frequency of <i>Bifidobacterium</i> and fecal n-valerate levels and between the changes in the fecal levels of iso-butyrate and iso-valerate. On the other hand, inverse correlations were detected between the changes in the frequency of <i>Clostridium</i> cluster IX and fecal iso-butyrate levels, between the changes in the frequency of <i>Clostridium</i> cluster IX and fecal iso-valerate levels, and between the changes in the frequencies of <i>Bacteroides</i> and <i>Clostridium</i> cluster IV plus subcluster XIVa. During the study period, the changes in fecal iso-butyrate and iso-valerate levels were positively correlated, and inverse correlations were seen between the changes in the frequency of <i>Clostridium</i> cluster IV and fecal propionate levels and between the changes in the frequencies of <i>Prevotella</i> and <i>Clostridium</i> subcluster XIVa. These findings suggest that hyperbaric conditions affect the fecal microbiota and fecal SCFA levels and that intestinal conditions reversibly deteriorate under hyperbaric conditions.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 4","pages":"168-175"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/98/8e/bmfh-40-168.PMC8484010.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39503445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of <i>Bifidobacterium bifidum</i> G9-1 in improving quality of life in patients with chronic constipation: a prospective intervention study.","authors":"Akiko Fuyuki, Takuma Higurashi, Takaomi Kessoku, Keiichi Ashikari, Tsutomu Yoshihara, Noboru Misawa, Michihiro Iwaki, Takashi Kobayashi, Hidenori Ohkubo, Masato Yoneda, Haruki Usuda, Koichiro Wada, Atsushi Nakajima","doi":"10.12938/bmfh.2020-073","DOIUrl":"10.12938/bmfh.2020-073","url":null,"abstract":"<p><p>Chronic constipation is a functional disorder that decreases a patient's quality of life (QOL). Because dysbiosis has been associated with constipation, we aimed to investigate the efficacy of <i>Bifidobacterium bifidum</i> G9-1 (BBG9-1) in improving QOL in patients with constipation. This was a prospective, single-center, non-blinded, single-arm feasibility trial. A total of 31 patients with constipation and decreased QOL received BBG9-1 treatment for 8 weeks, followed by a 2-week washout period. The primary endpoint was change in the overall Japanese version of the patient assessment of constipation of QOL (JPAC-QOL) score after probiotic administration relative to that at baseline. Secondary endpoints included changes in gut microbiota, stool consistency, frequency of bowel movement, degree of straining, sensation of incomplete evacuation, and frequency of rescue drug use. The overall JPAC-QOL scores and frequency of bowel movement significantly improved after BBG9-1 administration from those at baseline (p<0.01 and p<0.01, respectively). There were no statistically significant changes in other clinical symptoms. Subset analysis revealed that patients with initial Bristol Stool Form Scale stool types of <4 had improvements in stool consistency, a significant increase in the frequency of bowel movements, and a significant alleviation in the degree of straining, following BBG9-1 administration. At the genus and species levels, <i>Sarcina</i> and <i>Sarcina maxima</i> were significantly increased. Functional analysis showed that butanoate metabolism increased significantly, whereas methane metabolism decreased significantly. We concluded that BBG9-1 is safe and improves QOL in patients with constipation. The underlying improvements may be due to changes in stool consistency.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 2","pages":"105-114"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/95/ba/bmfh-40-105.PMC8099630.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38918649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increase in muscle mass associated with the prebiotic effects of 1-kestose in super-elderly patients with sarcopenia.","authors":"Kentaro Tominaga,&nbsp;Atsunori Tsuchiya,&nbsp;Oki Nakano,&nbsp;Yasutoshi Kuroki,&nbsp;Kentaro Oka,&nbsp;Ayaka Minemura,&nbsp;Asami Matsumoto,&nbsp;Motomichi Takahashi,&nbsp;Yoshihiro Kadota,&nbsp;Takumi Tochio,&nbsp;Yusuke Niwa,&nbsp;Tomoaki Yoshida,&nbsp;Masatoshi Sato,&nbsp;Takeshi Yokoo,&nbsp;Satoru Hashimoto,&nbsp;Junji Yokoyama,&nbsp;Jun Matsuzawa,&nbsp;Katsuya Fujimori,&nbsp;Shuji Terai","doi":"10.12938/bmfh.2020-063","DOIUrl":"https://doi.org/10.12938/bmfh.2020-063","url":null,"abstract":"<p><p>Sarcopenia causes functional disorders and decreases the quality of life. Thus, it has attracted substantial attention in the aging modern world. Dysbiosis of the intestinal microbiota is associated with sarcopenia; however, it remains unclear whether prebiotics change the microbiota composition and result in the subsequent recovery of muscle atrophy in elderly patients with sarcopenia. This study aimed to assess the effects of prebiotics in super-elderly patients with sarcopenia. We analyzed the effects of 1-kestose on the changes in the intestinal microbiota and body composition using a next-generation sequencer and a multi-frequency bioimpedance analysis device. The <i>Bifidobacterium longum</i> population was significantly increased in the intestine after 1-kestose administration. In addition, in all six patients after 12 weeks of 1-kestose administration, the skeletal muscle mass index was greater, and the body fat percentage was lower. This is the first study to show that administration of a prebiotic increased the population of <i>B. longum</i> in the intestinal microbiota and caused recovery of muscle atrophy in super-elderly patients with sarcopenia.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 3","pages":"150-155"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e0/bb/bmfh-40-150.PMC8279888.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39203675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of the gut microbiota and dysbiosis in children.","authors":"Shohei Akagawa, Yuko Akagawa, Sohsaku Yamanouchi, Takahisa Kimata, Shoji Tsuji, Kazunari Kaneko","doi":"10.12938/bmfh.2020-034","DOIUrl":"10.12938/bmfh.2020-034","url":null,"abstract":"<p><p>The gut microbiota resides in the human gastrointestinal tract, where it plays an important role in maintaining host health. Recent advancements in next-generation sequencing methods have revealed the link between dysbiosis (imbalance of the normal gut microbiota) and several diseases, as this imbalance can disrupt the symbiotic relationship between the host and associated microbes. Establishment of the gut microbiota starts <i>in utero</i> or just after birth, and its composition dramatically changes to an adult-like composition by 3 years of age. Because dysbiosis during childhood may persist through adulthood, it is crucial to acquire a balanced gut microbiota in childhood. Therefore, current studies have focused on the factors affecting the infant gut microbiota. This review discusses recent findings, including those from our studies, on how various factors, including the delivery mode, feeding type, and administration of drugs, including antibiotics, can influence the infant gut microbiota. Here, we also address future approaches for the prevention and restoration of dysbiosis in children.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 1","pages":"12-18"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/5f/bmfh-40-012.PMC7817514.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25315815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adhesion mechanisms of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> JCM 10602 to dietary fiber.","authors":"Maria Taniguchi,&nbsp;Minori Nambu,&nbsp;Yoshio Katakura,&nbsp;Shino Yamasaki-Yashiki","doi":"10.12938/bmfh.2020-003","DOIUrl":"https://doi.org/10.12938/bmfh.2020-003","url":null,"abstract":"<p><p>Adherence of probiotics to dietary fibers present in the intestinal tract may affect adhesion to intestinal epithelial cells. The properties of the adhesion of bifidobacteria to mucin or epithelial cells have been well studied; however, adhesion of bifidobacteria to dietary fiber has not been investigated. The adhesion ratio of six <i>Bifidobacterium</i> strains to cellulose and chitin was examined; among the strains, <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> JCM 10602 showed high adherence to both cellulose and chitin, and two strains showed high adherence to only chitin. The ratios of adhesion of <i>B. animalis</i> to cellulose and chitin were positively and negatively correlated with ionic strength, respectively. These data suggest that hydrophobic and electrostatic interactions are involved in the adhesion to cellulose and chitin, respectively. The adhesion ratios of the cells in the late logarithmic phase to cellulose and chitin decreased by approximately 40% and 70% of the cells in the early logarithmic phase, respectively. Furthermore, the adhesion ratio to cellulose decreased with increasing bile concentration regardless of the culture phase of the cells. On the other hand, the adhesion ratio to chitin of cells in the early logarithmic phase decreased with increasing bile concentration; however, that of cells in the late logarithmic phase increased slightly, suggesting that adhesins differ depending on the culture phase. Our results indicated the importance of considering adhesion to both dietary fibers and the intestinal mucosa when using bifidobacteria as probiotics.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 1","pages":"59-64"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/47/79/bmfh-40-059.PMC7817516.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25316305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the intake of non-live <i>Bifidobacterium bifidum</i> on the faecal IgA of full-term infants: a double-blind, randomised, placebo-controlled study.","authors":"Masaki Terahara,&nbsp;Yoshitaka Nakamura,&nbsp;Misato Tsuboi,&nbsp;Shinji Jinno,&nbsp;Takamitsu Tsukahara,&nbsp;Takao Miyake,&nbsp;Naoki Shimojo","doi":"10.12938/bmfh.2021-018","DOIUrl":"https://doi.org/10.12938/bmfh.2021-018","url":null,"abstract":"<p><p><i>Bifidobacterium bifidum</i> OLB6378 (OLB6378) was selected as a strain that enhances the production of secretory immunoglobulin A (IgA) <i>in vitro</i>. This ability of non-live OLB6378 has been shown by a clinical trial in preterm infants. In the present study, we examined whether non-live OLB6378 also enhances the production of secretory IgA, even in full-term infants. One hundred full-term infants were allocated to receive formula with (BbF group, 49 infants) or without non-live OLB6378 (PF group, 51 infants). Breastfeeding was prioritised, so infant formula was used for infants with breastfeeding difficulties. The intervention was initiated by five days of age. The faecal IgA concentration and OLB6378 level were determined at one, two, four, and eight weeks of age. Faecal IgA in the BbF group (1.04 ± 0.47 mg/g of faeces, n=45) was significantly higher than that in the PF group (0.85 ± 0.42 mg/g of faeces, n=49) at four weeks of age (p=0.047). OLB6378 was not detected in faeces at any age. This indicated that production of secretory IgA in full-term infants may also be enhanced by non-live OLB6378 intake.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 4","pages":"196-203"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a5/97/bmfh-40-196.PMC8484005.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39503448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gerobiotics: probiotics targeting fundamental aging processes.","authors":"Ying-Chieh Tsai, Li-Hao Cheng, Yen-Wenn Liu, One-Jang Jeng, Yuan-Kun Lee","doi":"10.12938/bmfh.2020-026","DOIUrl":"10.12938/bmfh.2020-026","url":null,"abstract":"<p><p>Aging is recognized as a common risk factor for many chronic diseases and functional decline. The newly emerging field of geroscience is an interdisciplinary field that aims to understand the molecular and cellular mechanisms of aging. Several fundamental biological processes have been proposed as hallmarks of aging. The proposition of the geroscience hypothesis is that targeting holistically these highly integrated hallmarks could be an effective approach to preventing the pathogenesis of age-related diseases jointly, thereby improving the health span of most individuals. There is a growing awareness concerning the benefits of the prophylactic use of probiotics in maintaining health and improving quality of life in the elderly population. In view of the rapid progress in geroscience research, a new emphasis on geroscience-based probiotics is in high demand, and such probiotics require extensive preclinical and clinical research to support their functional efficacy. Here we propose a new term, \"gerobiotics\", to define those probiotic strains and their derived postbiotics and para-probiotics that are able to beneficially attenuate the fundamental mechanisms of aging, reduce physiological aging processes, and thereby expand the health span of the host. We provide a thorough discussion of why the coining of a new term is warranted instead of just referring to these probiotics as anti-aging probiotics or with other similar terms. In this review, we highlight the needs and importance of the new field of gerobiotics, past and currently on-going research and development in the field, biomarkers for potential targets, and recommended steps for the development of gerobiotic products. Use of gerobiotics could be a promising intervention strategy to improve health span and longevity of humans in the future.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 1","pages":"1-11"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/e4/bmfh-40-001.PMC7817508.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25315814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbial composition of elderly women born in the Japanese longevity village Ogimi.","authors":"Hiroto Morita,&nbsp;Mutsuki Ichishima,&nbsp;Ipputa Tada,&nbsp;Hirotsugu Shiroma,&nbsp;Makoto Miyagi,&nbsp;Teppei Nakamura,&nbsp;Hiroshi Tanaka,&nbsp;Shinya Ikematsu","doi":"10.12938/bmfh.2019-055","DOIUrl":"https://doi.org/10.12938/bmfh.2019-055","url":null,"abstract":"<p><p>Ogimi is one of Japan's longevity villages and is located in Okinawa Prefecture. In this study, we focused on the elderly women living in the village, classified them into two groups based on whether or not they lived in Ogimi during the first 3 years of their lives, and compared the gut microbiota between the two groups. There were no differences in alpha and beta diversity; however, we found that the elderly women who lived in Ogimi during the first 3 years of their lives had a higher rate of <i>Akkermansia muciniphila</i> colonization in their guts.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 1","pages":"75-79"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/72/32/bmfh-40-075.PMC7817513.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25316307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methionine utilization by bifidobacteria: possible existence of a reverse transsulfuration pathway.","authors":"Masaru Wada,&nbsp;Satoru Fukiya,&nbsp;Azusa Suzuki,&nbsp;Nanae Matsumoto,&nbsp;Miki Matsuo,&nbsp;Atsushi Yokota","doi":"10.12938/bmfh.2020-031","DOIUrl":"https://doi.org/10.12938/bmfh.2020-031","url":null,"abstract":"<p><p>Although bifidobacteria are already widely used as beneficial microbes with health-promoting effects, their amino acid utilization and metabolism are not yet fully understood. Knowledge about the metabolism of sulfur-containing amino acids in bifidobacteria is especially limited. In this study, we tested the methionine utilization ability of several bifidobacterial strains when it was the sole available sulfur source. Although bifidobacteria have long been predominantly considered to be cysteine auxotrophs, we showed that this is not necessarily the case.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 1","pages":"80-83"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a9/2c/bmfh-40-080.PMC7817509.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25316308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional role of surface layer proteins of <i>Lactobacillus acidophilus</i> L-92 in stress tolerance and binding to host cell proteins.","authors":"Taketo Wakai,&nbsp;Chie Kano,&nbsp;Harma Karsens,&nbsp;Jan Kok,&nbsp;Naoyuki Yamamoto","doi":"10.12938/bmfh.2020-005","DOIUrl":"https://doi.org/10.12938/bmfh.2020-005","url":null,"abstract":"<p><p><i>Lactobacillus acidophilus</i> surface layer proteins (SLPs) self-assemble into a monolayer that is non-covalently bound to the outer surface of the cells. There they are in direct contact with the environment, environmental stressors and gut components of the host in which the organism resides. The role of <i>L. acidophilus</i> SLPs is not entirely understood, although SLPs seem to be essential for bacterial growth. We constructed three <i>L. acidophilus</i> L-92 strains, each expressing a mutant of the most abundant SLP, SlpA. Each carried a 12-amino acid c-myc epitope substitution at a different position in the protein. A strain was also obtained that expressed the SlpA paralog SlpB from an originally silent <i>slpB</i> gene. All four strains behaved differently with respect to growth under various stress conditions, such as the presence of salt, ox gall or ethanol, suggesting that SlpA affects stress tolerance in <i>L. acidophilus</i> L-92. Also, the four mutants showed differential <i>in vitro</i> binding ability to human host cell proteins such as uromodulin or dendritic cell (DC)-specific intercellular adhesion molecule-3 grabbing non-integrin (DC-SIGN). Furthermore, co-culture of murine immature DCs with a mutant strain expressing one of the recombinant SlpA proteins changed the concentrations of the cytokines IL-10 and IL-12. Our data suggest that SlpA and SlpB of <i>L. acidophilus</i> participate in bacterial stress tolerance and binding to uromodulin or DC-SIGN, possibly leading to effective immune-modification.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":"40 1","pages":"33-42"},"PeriodicalIF":3.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b1/24/bmfh-40-033.PMC7817507.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25316302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}