在啮齿动物模型中肥胖症和2型糖尿病的肠道微生物群特征。

IF 3.1 4区 医学 Q2 Agricultural and Biological Sciences
Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2020-10-10 DOI:10.12938/bmfh.2019-031
Khalid S Ibrahim, Nowara Bourwis, Sharron Dolan, Sue Lang, Janice Spencer, John A Craft
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引用次数: 12

摘要

各种研究表明,肠道微生物组与宿主相互作用,可能在肥胖和2型糖尿病(T2D)的病因学中发挥重要作用。据推测,肥胖和T2D中的细菌群落与对照组不同,并损害宿主和微生物群之间的正常相互作用。通过分别饲喂高脂肪饮食或高脂肪饮食加单次低剂量链脲佐菌素,大鼠发生肥胖和T2D。通过宏基因组16S rRNA测序和生物信息学技术,建立了微生物组谱及其代谢潜力。肥胖和T2D的分类和预测代谢相关基因与对照组明显不同,甚至彼此之间也存在差异。T2D大鼠的多样性减少,但肥胖大鼠没有。在肥胖和T2D大鼠中发现了可能损害宿主肠道屏障完整性的因素,包括预测的细菌丁酸盐产量减少。肥胖和T2D大鼠通过abc转运体和碳水化合物代谢增加能量提取的能力增强。T2D以促炎分子增多为特征。虽然肥胖和T2D表现出明显的差异,但结果表明,在这两种情况下,拟杆菌和蓝芽胞杆菌种类都增加了,这表明可能存在机制联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Characterisation of gut microbiota of obesity and type 2 diabetes in a rodent model.

Characterisation of gut microbiota of obesity and type 2 diabetes in a rodent model.

Characterisation of gut microbiota of obesity and type 2 diabetes in a rodent model.

Characterisation of gut microbiota of obesity and type 2 diabetes in a rodent model.

Various studies have suggested that the gut microbiome interacts with the host and may have a significant role in the aetiology of obesity and Type 2 Diabetes (T2D). It was hypothesised that bacterial communities in obesity and T2D differ from control and compromise normal interactions between host and microbiota. Obesity and T2D were developed in rats by feeding a high-fat diet or a high-fat diet plus a single low-dose streptozotocin administration, respectively. The microbiome profiles and their metabolic potentials were established by metagenomic 16S rRNA sequencing and bioinformatics. Taxonomy and predicted metabolism-related genes in obesity and T2D were markedly different from controls and indeed from each other. Diversity was reduced in T2D but not in Obese rats. Factors likely to compromise host intestinal, barrier integrity were found in Obese and T2D rats including predicted, decreased bacterial butyrate production. Capacity to increase energy extraction via ABC-transporters and carbohydrate metabolism were enhanced in Obese and T2D rats. T2D was characterized by increased proinflammatory molecules. While obesity and T2D show distinct differences, results suggest that in both conditions Bacteroides and Blautia species were increased indicating a possible mechanistic link.

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来源期刊
Bioscience of Microbiota, Food and Health
Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
5.50
自引率
3.20%
发文量
24
期刊介绍: Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.
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