纳豆中枯草芽孢杆菌G8分离株的纤溶特性。

IF 3.1 4区 医学 Q2 Agricultural and Biological Sciences
Bioscience of Microbiota, Food and Health Pub Date : 2021-01-01 Epub Date: 2021-02-20 DOI:10.12938/bmfh.2020-071
Reinhard Pinontoan, Elvina, Astia Sanjaya, Juandy Jo
{"title":"纳豆中枯草芽孢杆菌G8分离株的纤溶特性。","authors":"Reinhard Pinontoan,&nbsp;Elvina,&nbsp;Astia Sanjaya,&nbsp;Juandy Jo","doi":"10.12938/bmfh.2020-071","DOIUrl":null,"url":null,"abstract":"<p><p>Due to the high prevalence of vascular obstructive diseases, discovering potent, safe, and affordable fibrinolytic agents is of importance. There is particular interest concerning the use of functional foods that have a fibrinolytic activity, such as natto, a Japanese fermented soy-based product made with <i>Bacillus subtilis</i> (natto) strain BEST195. We recently isolated another bacterial strain from natto commercialized in Indonesia, <i>B. subtilis</i> G8, which has proven to exert fibrinolytic activity. Herein, a further characterization of <i>B. subtilis</i> G8 was assessed through a comparison with commercialized nattokinase, the major fibrinolytic enzyme of <i>B. subtilis</i>, by utilizing various <i>in vitro</i> fibrinolytic assays, namely whole blood clot lysis, euglobulin clot lysis, the fibrin plate method, and zymography. Both nattokinase and <i>B. subtilis</i> G8 were able to dissolve both whole blood and euglobulin clots. Furthermore, both nattokinase and <i>B. subtilis</i> G8 were able to lyse blood clots, presumably due to their ability to directly lyse fibrin. Finally, a crude extract of <i>B. subtilis</i> G8 displayed six zymogram bands of approximately 42.0, 35.5, 30.8, 26.7, 20.0, and 13.7 kDa, with the strongest activity observed at 20.0 kDa. This indicates that <i>B. subtilis</i> G8 contained several fibrinolytic enzymes, which might have comprised nattokinase and other fibrinolytic enzymes. In summary, we demonstrated that a crude extract of <i>B. subtilis</i> G8 has potent fibrinolytic activity and that the activity was mediated by various fibrinolytic enzymes.</p>","PeriodicalId":8867,"journal":{"name":"Bioscience of Microbiota, Food and Health","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3e/38/bmfh-40-144.PMC8279889.pdf","citationCount":"9","resultStr":"{\"title\":\"Fibrinolytic characteristics of <i>Bacillus subtilis</i> G8 isolated from natto.\",\"authors\":\"Reinhard Pinontoan,&nbsp;Elvina,&nbsp;Astia Sanjaya,&nbsp;Juandy Jo\",\"doi\":\"10.12938/bmfh.2020-071\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Due to the high prevalence of vascular obstructive diseases, discovering potent, safe, and affordable fibrinolytic agents is of importance. There is particular interest concerning the use of functional foods that have a fibrinolytic activity, such as natto, a Japanese fermented soy-based product made with <i>Bacillus subtilis</i> (natto) strain BEST195. We recently isolated another bacterial strain from natto commercialized in Indonesia, <i>B. subtilis</i> G8, which has proven to exert fibrinolytic activity. Herein, a further characterization of <i>B. subtilis</i> G8 was assessed through a comparison with commercialized nattokinase, the major fibrinolytic enzyme of <i>B. subtilis</i>, by utilizing various <i>in vitro</i> fibrinolytic assays, namely whole blood clot lysis, euglobulin clot lysis, the fibrin plate method, and zymography. Both nattokinase and <i>B. subtilis</i> G8 were able to dissolve both whole blood and euglobulin clots. Furthermore, both nattokinase and <i>B. subtilis</i> G8 were able to lyse blood clots, presumably due to their ability to directly lyse fibrin. Finally, a crude extract of <i>B. subtilis</i> G8 displayed six zymogram bands of approximately 42.0, 35.5, 30.8, 26.7, 20.0, and 13.7 kDa, with the strongest activity observed at 20.0 kDa. This indicates that <i>B. subtilis</i> G8 contained several fibrinolytic enzymes, which might have comprised nattokinase and other fibrinolytic enzymes. In summary, we demonstrated that a crude extract of <i>B. subtilis</i> G8 has potent fibrinolytic activity and that the activity was mediated by various fibrinolytic enzymes.</p>\",\"PeriodicalId\":8867,\"journal\":{\"name\":\"Bioscience of Microbiota, Food and Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3e/38/bmfh-40-144.PMC8279889.pdf\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience of Microbiota, Food and Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.12938/bmfh.2020-071\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/2/20 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience of Microbiota, Food and Health","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.12938/bmfh.2020-071","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/2/20 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 9

摘要

由于血管阻塞性疾病的高患病率,发现有效的、安全的、负担得起的纤溶药物是很重要的。人们对具有纤维蛋白溶解活性的功能性食品的使用特别感兴趣,例如纳豆,这是一种由枯草芽孢杆菌(纳豆)菌株BEST195制成的日本发酵大豆产品。我们最近从印度尼西亚商业化的纳豆中分离出另一种菌株,枯草芽孢杆菌G8,已被证明具有纤溶活性。本文通过与商业化的纳豆激酶(枯草芽孢杆菌的主要纤维蛋白溶解酶)进行比较,利用各种体外纤维蛋白溶解试验,即全血凝块溶解、优球蛋白凝块溶解、纤维蛋白板法和酶谱法,进一步评估枯草芽孢杆菌G8的特性。纳豆激酶和枯草芽孢杆菌G8均能溶解全血和euglobulin凝块。此外,纳豆激酶和枯草芽孢杆菌G8都能溶解血凝块,可能是由于它们直接溶解纤维蛋白的能力。结果表明,枯草芽孢杆菌G8粗提物在约42.0、35.5、30.8、26.7、20.0和13.7 kDa处有6个酶谱带,其中在20.0 kDa处活性最强。这表明枯草芽孢杆菌G8含有多种纤溶酶,可能包括纳豆激酶和其他纤溶酶。综上所述,我们证明枯草芽孢杆菌G8粗提物具有较强的纤溶活性,且该活性是由多种纤溶酶介导的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Fibrinolytic characteristics of <i>Bacillus subtilis</i> G8 isolated from natto.

Fibrinolytic characteristics of <i>Bacillus subtilis</i> G8 isolated from natto.

Fibrinolytic characteristics of <i>Bacillus subtilis</i> G8 isolated from natto.

Fibrinolytic characteristics of Bacillus subtilis G8 isolated from natto.

Due to the high prevalence of vascular obstructive diseases, discovering potent, safe, and affordable fibrinolytic agents is of importance. There is particular interest concerning the use of functional foods that have a fibrinolytic activity, such as natto, a Japanese fermented soy-based product made with Bacillus subtilis (natto) strain BEST195. We recently isolated another bacterial strain from natto commercialized in Indonesia, B. subtilis G8, which has proven to exert fibrinolytic activity. Herein, a further characterization of B. subtilis G8 was assessed through a comparison with commercialized nattokinase, the major fibrinolytic enzyme of B. subtilis, by utilizing various in vitro fibrinolytic assays, namely whole blood clot lysis, euglobulin clot lysis, the fibrin plate method, and zymography. Both nattokinase and B. subtilis G8 were able to dissolve both whole blood and euglobulin clots. Furthermore, both nattokinase and B. subtilis G8 were able to lyse blood clots, presumably due to their ability to directly lyse fibrin. Finally, a crude extract of B. subtilis G8 displayed six zymogram bands of approximately 42.0, 35.5, 30.8, 26.7, 20.0, and 13.7 kDa, with the strongest activity observed at 20.0 kDa. This indicates that B. subtilis G8 contained several fibrinolytic enzymes, which might have comprised nattokinase and other fibrinolytic enzymes. In summary, we demonstrated that a crude extract of B. subtilis G8 has potent fibrinolytic activity and that the activity was mediated by various fibrinolytic enzymes.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Bioscience of Microbiota, Food and Health
Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
5.50
自引率
3.20%
发文量
24
期刊介绍: Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信