Biopolymers最新文献_第5页

Imparting of Nearly Superparamagnetic Properties to Cryogel Scaffolds With Mesoporous MNPs for Magneto-Sensitive Tissue Engineering Strategies 用介孔 MNPs 为冷凝胶支架赋予近超顺磁性能，以实现磁敏感组织工程策略。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-19 DOI: 10.1002/bip.23623

Didem Demir, Fatma Ulusal, Hasan Ulusal, Seda Ceylan, Sibel Dağlı, Nalan Özdemir, Mehmet Tarakçıoğlu

{"title":"Imparting of Nearly Superparamagnetic Properties to Cryogel Scaffolds With Mesoporous MNPs for Magneto-Sensitive Tissue Engineering Strategies","authors":"Didem Demir, Fatma Ulusal, Hasan Ulusal, Seda Ceylan, Sibel Dağlı, Nalan Özdemir, Mehmet Tarakçıoğlu","doi":"10.1002/bip.23623","DOIUrl":"10.1002/bip.23623","url":null,"abstract":"This work reports the assembly of mesoporous iron oxide nanoparticles (meso-MNPs) with cryogel scaffolds composed of chitosan and gelatin. Meso-MNPs with a particle size ranging from 2 and 50 nm, a surface area of 140.52 m2 g−1, and a pore volume of 0.27 cm3 g−1 were synthesized on a porous SiO2 template in the presence of PEG 6000 followed by leaching of SiO2. Different ratios of meso-MNPs were successfully incorporated into chitosan:gelatin cryogels up to an amount equivalent to the entire amount of polymer. The morphological structure and physicochemical properties of the cryogels were directly affected by the amount of MNPs. VSM curves showed that all composite cryogels could be magnetized by applying a magnetic field. In the context of the safety of magnetic cryogel scaffolds for use in biomedicine, it is important to note that all values are below the exposure limit for static magnetic fields, and according to cytotoxicity data, scaffolds containing meso-MNPs showed nontoxicity with cell viability ranging from 150% to 275%. In addition, microbial analysis with gram-negative and gram-positive bacteria showed that the scaffolds exhibited activity against these bacteria.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship Between Cryoprotectant Potential and Protein Hydration in Aqueous Zwitterionic Solutions 水性齐聚物溶液中的低温保护电位与蛋白质水合作用之间的关系

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-17 DOI: 10.1002/bip.23622

Takahiro Takekiyo, Shuto Yamada, Tetsuya Hirata, Takeru Ishizaki, Kosuke Kuroda, Yukihiro Yoshimura

{"title":"Relationship Between Cryoprotectant Potential and Protein Hydration in Aqueous Zwitterionic Solutions","authors":"Takahiro Takekiyo, Shuto Yamada, Tetsuya Hirata, Takeru Ishizaki, Kosuke Kuroda, Yukihiro Yoshimura","doi":"10.1002/bip.23622","DOIUrl":"10.1002/bip.23622","url":null,"abstract":"<div>\u0000 \u0000 The cryoprotectant potential of 3-(1-(2-(2-methoxyethoxy)ethyl)imidazol-3-io)butane-1-carboxylate (OE2imC3C) for proteins necessitates assessment to elucidate its relationship with protein hydration. To reveal this relationship, we assessed the protein stability (pre-freezing and post-thawing) and melting behavior in dilute aqueous protein–OE2imC3C solutions containing varying mole fractions (x) of OE2imC3C (x = 0, 7.7 × 10−3, and 1.7 × 10−2) using Fourier-transform infrared (FTIR) and near-UV circular dichroism (near-UV CD) spectroscopy and differential scanning calorimetry (DSC) techniques. Following freezing/thawing using a deep freezer, protein stability in aqueous OE2imC3C solutions (x = 1.7 × 10−2) preserved the folded state owing to the protein–OE2imC3C interaction, whereas stability at x = 7.7 × 10−3 was reduced. These results indicate that the protein cryoprotectant potential in aqueous OE2imC3C solutions at x = 1.7 × 10−2 is higher than that at x = 7.7 × 10−3, owing to the preferential binding of OE2imC3C with proteins.\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Alkali Treatment Conditions of Ramie Fabrics Using Box–Behnken Method 使用箱式贝肯法优化苎麻织物的碱处理条件

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-12 DOI: 10.1002/bip.23621

Bewuket Teshome Wagaye, Jiansheng Guo

{"title":"Optimization of Alkali Treatment Conditions of Ramie Fabrics Using Box–Behnken Method","authors":"Bewuket Teshome Wagaye, Jiansheng Guo","doi":"10.1002/bip.23621","DOIUrl":"10.1002/bip.23621","url":null,"abstract":"<div>\u0000 \u0000 Ramie is a widely used plant fiber for making textiles and reinforcement in biodegradable composites. Pretreating cellulosic fibers with alkali before producing composites is increasingly used to enhance adhesion with polymeric resin. In this work, response surface methodology (RSM) based on the Box–Behnken technique was utilized to investigate the impact of independent variables on ramie fabric characteristics and determine the optimal treatment condition. The impact of alkali concentration, treatment time, and temperature on the breaking load and elongation at break of woven ramie fabrics were evaluated using Design-Expert software, which established the design matrix and analyzed the experimental data employing numerical and graphical optimization methods. Moreover, the impact of alkali treatment conditions on the surface morphology, structural change of ramie fabrics, and thermal properties was investigated. Based on the analysis of variance (ANOVA) results, the suggested quadratic models can adequately predict the breaking load and elongation at break of the ramie woven fabrics within the range of conditions applied in this investigation. The RSM revealed that an alkali concentration of 6.12%, a treatment time of 30 min, and a temperature of 39.13°C resulted in an optimum treatment condition with a breaking load of 518.28 N and elongation at break of 23.36%.\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of High Toughness Poly(3-Hydroxypropionate)-Based Block Copolymers With Poly(D-2-Hydroxybutyrate) and Poly(D-Lactate) Segments Using Evolved Monomer Sequence-Regulating Polyester Synthase 利用进化单体序列调节聚酯合成酶生物合成具有聚（D-2-羟基丁酸酯）和聚（D-乳酸酯）段的高韧性聚（3-羟基丙酸酯）基嵌段共聚物

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-08 DOI: 10.1002/bip.23618

Tomoya Kawakami, Hiroya Tomita, Phan Thi Hien, Ken'ichiro Matsumoto

{"title":"Biosynthesis of High Toughness Poly(3-Hydroxypropionate)-Based Block Copolymers With Poly(D-2-Hydroxybutyrate) and Poly(D-Lactate) Segments Using Evolved Monomer Sequence-Regulating Polyester Synthase","authors":"Tomoya Kawakami, Hiroya Tomita, Phan Thi Hien, Ken'ichiro Matsumoto","doi":"10.1002/bip.23618","DOIUrl":"10.1002/bip.23618","url":null,"abstract":"This study synthesized poly(3-hydroxypropionate) [P(3HP)]-containing polyhydroxyalkanoate (PHA) block copolymers, P(3HP)-b-P[2-hydroxybutyrate (2HB)] and P(3HP)-b-P(D-lactate) (PDLA), using Escherichia coli. The cells expressing an evolved sequence-regulating PHA synthase, PhaCARNDFH, and propionyl-CoA transferase were cultured with the supplementation of the corresponding monomer precursors in the medium. The block structure of P(3HP)-b-PDLA was confirmed by proton nuclear magnetic resonance analysis and solvent fractionation. The molecular weights of the polymers were in the range of 0.8–2.8 × 105. The solvent-cast polymer films were subjected to isothermal treatment to promote phase separation and crystallization and were subsequently melt-quenched to produce an amorphous phase. The melt-quenched P(3HP)-b-P(2HB) film exhibited a high elongation at break (1153%), resulting in a toughness of 181 MJ/m3. The solvent-cast film of P(3HP)-b-65 mol% PDLA exhibited partial elastic deformation, in which the P(3HP) phase functioned as a soft segment. The melt-quenching of the polymer resulted in embrittlement presumably due to the high lactate fraction. Overall, the P(3HP)-based block copolymers exhibited several mechanical properties depending on the higher-order structure of the polymer and the properties of the P(2-hydroxyalkanoate) segments. This study findings show that P(3HP)-b-P(2HB) and P(3HP)-b-PDLA can function excellently if their microstructures are properly controlled.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.23618","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141938921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and Characterization of Durian Husk-Based Biocomposite Films Reinforced With Nanocellulose From Corn Husk and Pineapple Leaf 以玉米皮和菠萝叶纳米纤维素为增强材料的榴莲壳基生物复合薄膜的制备与表征

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-08 DOI: 10.1002/bip.23619

Nattapron Siribanluehan, Piyachat Wattanachai

{"title":"Preparation and Characterization of Durian Husk-Based Biocomposite Films Reinforced With Nanocellulose From Corn Husk and Pineapple Leaf","authors":"Nattapron Siribanluehan, Piyachat Wattanachai","doi":"10.1002/bip.23619","DOIUrl":"10.1002/bip.23619","url":null,"abstract":"<div>\u0000 \u0000 This research explores the integration of corn husk nanocellulose (CHNc) and pineapple leaf nanocellulose (PLNc) as reinforcing agents in a carboxymethyl cellulose-based film derived from durian husk (CMCDH). Through a solvent-casting method, composite films were fabricated with varying nanocellulose contents (15, 30, and 45 wt%). Analysis using Fourier transform infrared spectroscopy and x-ray diffraction confirmed the effectiveness of alkaline and bleaching treatments in eliminating noncellulosic components. Transmission electron microscopy image revealed the rod-like morphology of CHNc and PLNc, with dimensions approximately 206.5 × 7.2 nm and 150.7 × 6.5 nm, respectively. The inclusion of nanocellulose decreased the transparency of CMCDH films while enhancing their tensile strength, thermal stability, and water vapor transmission rate. Notably, CMCDH/PLNc(30%) exhibited the highest tensile strength at 5.06 ± 0.83 MPa, representing a remarkable 220% increase compared to CMCDH biofilm. Thermogravimetric analysis and differential scanning calorimeter results indicated that nanocellulose incorporation delayed the film's decomposition temperature by approximately 10°C. Moreover, CMCDH/PLNc(30%) demonstrated the lowest water vapor transmission rate, marking a 20% improvement. However, the film's properties were compromised at the highest nanocellulose content (45 wt%) due to observed fiber aggregation, as revealed by scanning electron microscopy analysis.\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Customized Modification of Welan Gum Properties Through Controllable Grafting of Acrylamide 通过丙烯酰胺的可控接枝定制改良韦兰胶性能。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-07 DOI: 10.1002/bip.23620

Guorui Xu, Jianye Li, Xiang Li, Yongkang Jia, Yajie Song, Jiming Wang, Lei Wang, Haibo Zhang

{"title":"Customized Modification of Welan Gum Properties Through Controllable Grafting of Acrylamide","authors":"Guorui Xu, Jianye Li, Xiang Li, Yongkang Jia, Yajie Song, Jiming Wang, Lei Wang, Haibo Zhang","doi":"10.1002/bip.23620","DOIUrl":"10.1002/bip.23620","url":null,"abstract":"<div>\u0000 \u0000 Welan gum (WG) has a wide range of applications, but it is not yet suitable for applications such as oil recovery profile control that have complex requirements for viscosity, gelation properties, and so forth. Grafting modification is an important strategy for improving the property of WG, but there are few reports on controllable modification of WG to customize it for specific application. Acrylamide (AM) dosage was identified as the key factor affecting the grafting ratio of AM onto WG by a uniform experimental design. The grafting ratio can be directly adjusted between 99% and 378% based on the positive correlation with dosage of AM, and viscosity can be adjusted between 206 and 327 mPa s based on the negative correlation with grafting ratio. The 50% weight loss temperature of W11 with a grafting ratio of 110% raised from 314 to 336°C after grafting. The viscosity of the hydrogel formed with WG11 reached 15,654 mPa s, nearly nine times higher than that of unmodified WG. In addition, the gelation time can be controlled within 5 days, so that it can be injected to the optimal area in oilfield profile, avoiding pipeline blockage. This study enables adjusting viscosity of WG grafted with AM by controlling the grafting rate, and enhances gelation performance and thermal stability of WG, which will expand the application of WG in oil recovery and other fields.\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermite frass biomass and surface modified biowaste coir fiber reinforced biocomposites—Conversion of waste to useful products 热熔碎屑生物质和表面改性生物废弃物椰壳纤维增强生物复合材料--将废弃物转化为有用产品。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-07-19 DOI: 10.1002/bip.23616

Justin Abraham Sajin, Vaithilingam Shanmugavelayutham Sreenivasan, Brailson Mansingh Bright, Murugan Sundaram Senthil Saravanan, Trijo Tharayil, Raveendra Kurup Anish, Manikandan Natarajan, Govindarajan Bharathiraja, Joseph Selvi Binoj

{"title":"Thermite frass biomass and surface modified biowaste coir fiber reinforced biocomposites—Conversion of waste to useful products","authors":"Justin Abraham Sajin, Vaithilingam Shanmugavelayutham Sreenivasan, Brailson Mansingh Bright, Murugan Sundaram Senthil Saravanan, Trijo Tharayil, Raveendra Kurup Anish, Manikandan Natarajan, Govindarajan Bharathiraja, Joseph Selvi Binoj","doi":"10.1002/bip.23616","DOIUrl":"10.1002/bip.23616","url":null,"abstract":"Polymer composites are known for its light weight and specific mechanical characteristics. This study examines sodium hydroxide (NaOH)-treated coir fiber, an agro-leftover, stuffed in a polyester matrix with termite frass powder, a bio-leftover for possible use in light-weight structural applications. Composite samples were made using compression molding and NaOH-treated coir fiber reinforced hybrid polymer composite (TCRHPC) with 40 wt% treated coir fiber and 1, 2, 3, and 4 wt% termite frass powder. TCRHPC samples mechanical, water captivation, tribological, and thermal properties were affected by termite frass powder wt%. The TCRHPC sample with 3 wt% termite frass powder has excellent mechanical properties, which improved by tensile (41.6%), flexural (28.57%), impact (43.7%), and hardness (18.84%) properties. With perfect water captivation and low weight increases in normal water (0.017 g), seawater (0.015 g), and NaOH solution (0.010 g), the identical composite sample with thermal stability up to 238°C also reduced wear mass by 5.27%. Conversely, filler agglomeration and heterogeneous dispersion in composite sample impair thermo-mechanical characteristics of TCRHPC containing 4 wt% termite frass powder. The bonding among polyester, treated coir fiber, and termite frass powder in composites were appraised with the aid of fractographic images of TCRHPC samples. The results show that TCRHPC material suits well for support structures requiring lesser weight.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141730964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new application of avocado oil to enrich the biological activities of polycaprolactone membranes for tissue engineering 牛油果油在丰富组织工程聚己内酯膜生物活性方面的新应用。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-07-19 DOI: 10.1002/bip.23617

Merve Çapkın Yurtsever, Selin Aydoğan, Zeynep İyigündoğdu, Alican Cömertpay, Didem Demir, Seda Ceylan

{"title":"A new application of avocado oil to enrich the biological activities of polycaprolactone membranes for tissue engineering","authors":"Merve Çapkın Yurtsever, Selin Aydoğan, Zeynep İyigündoğdu, Alican Cömertpay, Didem Demir, Seda Ceylan","doi":"10.1002/bip.23617","DOIUrl":"10.1002/bip.23617","url":null,"abstract":"The metabolites synthesized by plants to protect themselves serves as natural antimicrobial agents used in biomaterials. In this study, avocado oil (AO), was incorporated as a plant source and natural antimicrobial agent into polycaprolactone (PCL) membranes. The effects of varying AO ratios (25, 50, and 100 wt%.—PCL@25AO, PCL@50AO, PCL@100AO) on PCL membrane morphology, chemical structure, wettability, antimicrobial activity, and cell viabilities were investigated. It was demonstrated that the AO acts as a pore-forming agent in solvent-casted membranes. Young's modulus of the membranes varied between 602.68 and 31.92 MPa and more flexible membranes were obtained with increasing AO content. Inhibition zones of AO were recorded between 7.86 and 13.97 mm against clinically relevant microbial strains including bacteria, yeast, and fungi. Antimicrobial activity of AO was retained in PCL membranes at all ratios. Resazurin assay indicated that PCL@25AO membranes were cytocompatible with mouse fibroblast cells (L929 cell line) on day 6 showing 72.4% cell viability with respect to neat PCL membranes. Viability results were supported by scanning electron microscopy images and DAPI staining. The overall results of this study highlight the potential of PCL@25AO membranes as a biomaterial with antimicrobial properties, cytocompatibility, and mechanical strength suitable for various biomedical applications.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141730963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defined folding pattern of poly(rG) supports inherent ability to encode biological information 定义的 poly(rG) 折叠模式支持编码生物信息的内在能力。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-07-14 DOI: 10.1002/bip.23615

Nickolas Kankia, Levan Lomidze, Skylar Stevenson, Karin Musier-Forsyth, Besik Kankia

{"title":"Defined folding pattern of poly(rG) supports inherent ability to encode biological information","authors":"Nickolas Kankia, Levan Lomidze, Skylar Stevenson, Karin Musier-Forsyth, Besik Kankia","doi":"10.1002/bip.23615","DOIUrl":"10.1002/bip.23615","url":null,"abstract":"The RNA World hypothesis posits that RNA can represent a primitive life form by reproducing itself and demonstrating catalytic activity. However, this hypothesis is incapable of addressing several major origin-of-life (OoL) questions. A recently described paradox-free alternative OoL hypothesis, the Quadruplex (G4) World, is based on the ability of poly(dG) to fold into a stable architecture with an unambiguous folding pattern using G-tetrads as building elements. Because of the folding pattern of three G-tetrads and single-G loops, dG15 is programmable and has the capability to encode biological information. Here, we address two open questions of the G4 World hypothesis: (1) Does RNA follow the same folding pattern as DNA? (2) How do stable quadruplexes evolve into the present-day system of information transfer, which is based on Watson-Crick base pair complementarity? To address these questions, we systematically studied the thermodynamic and optical properties of both DNA and RNA G15- and G3T (GGGTGGGTGGGTGGG)-derived sequences. Our study revealed that similar to DNA sequences, RNAs adopt quadruplexes with only three G-tetrads. Thus, both poly(dG) and poly(rG) possess inherent ability to fold into 3D quadruplex architecture with strictly defined folding pattern. The study also revealed that despite high stability of both DNA and RNA quadruplexes, they are vulnerable to single-nucleotide substitutions, which drop the thermal stability by ~40°C and can facilitate introduction of the complementarity principle into the G4 World.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141615881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the physicochemical, antimicrobial and antioxidant properties of chitosan film containing zero-valent iron nanoparticles and oregano essence 研究含有零价铁纳米粒子和牛至精的壳聚糖薄膜的理化、抗菌和抗氧化特性。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-07-12 DOI: 10.1002/bip.23614

Farzaneh Khalil Khodaparast, Sajad Pirsa, Farzad Mirab Toupchi, Forogh Mohtarami

{"title":"Investigating the physicochemical, antimicrobial and antioxidant properties of chitosan film containing zero-valent iron nanoparticles and oregano essence","authors":"Farzaneh Khalil Khodaparast, Sajad Pirsa, Farzad Mirab Toupchi, Forogh Mohtarami","doi":"10.1002/bip.23614","DOIUrl":"10.1002/bip.23614","url":null,"abstract":"The problems caused by the pollution of the environment by petroleum polymers in recent years have caused researchers to think of replacing petroleum polymers with biodegradable and natural polymers. The aim of this research was to produce composite film of chitosan (Chit)/zero-valent iron (Fe) nanoparticles/oregano essence (Ess) (Chit/Fe/Ess). Central composite design was used to study physical, morphological, antioxidant and antimicrobial properties of films. The results showed that with the increase of iron nanoparticles and oregano essence, the thickness of the film increased. The moisture, solubility and water vapor permeability of the film decreased with the increase of iron nanoparticles and oregano essence. The results of the mechanical test showed that with the increase of iron nanoparticles and oregano essence, the tensile strength and elongation at break point decreased. Iron nanoparticles and oregano essence increased significantly the antioxidant activity of the film. The results of the antimicrobial activity of the prepared films show that the addition of iron nanoparticles and oregano essence enhanced the antimicrobial activity of the film against Escherichia coli and Staphylococcus aureus. X-ray diffraction analysis showed that iron nanoparticles were physically combined with chitosan polymer. Fourier transform infrared (FTIR) results confirmed the physical presence of iron nanoparticles and oregano essence in the polymer matrix. The results of scanning electron microscopy (SEM) showed that the surface of nanocomposite films is more heterogeneous than chitosan. Iron nanoparticles and oregano essence could delay the thermal decomposition of chitosan and increase the thermal stability of chitosan film.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0