Biopolymers最新文献

{"title":"Edible Bird's Nest Peptide- an Active Peptide With Potential in Promoting Skin Repair","authors":"Weijuan Bai,&nbsp;Fang Zheng,&nbsp;Xuncai Liu,&nbsp;Baozhong Guo,&nbsp;Xiaoqian Yin,&nbsp;Jianmei Lian,&nbsp;Qunyan Fan,&nbsp;Fenghong Deng","doi":"10.1002/bip.70055","DOIUrl":"10.1002/bip.70055","url":null,"abstract":"<div>\u0000 \u0000 <p>Skin damage and aging are caused by various factors, including UV radiation and air pollution. Edible bird's nest peptide (EBNP) was a type of safe short molecule peptide that could protect the skin by providing anti-oxidation and anti-inflammatory properties. In this study, the effect of EBNP on pro-tissue regeneration was examined. The findings revealed that EBNP contained epidermal growth factor (EGF) and could stimulate wound healing in cells and zebrafish larvae. The mechanism of skin repairing was further investigated. On the one hand, EBNP increased the synthesis of structural proteins and remodelled the extracellular matrix (ECM) by up-regulating the expression of COL1A1 gene in cells as well as col1a1b, eln1, and eln2 genes in zebrafish larvae. Furthermore, EBNP had an anti-inflammatory effect, as evidenced by its capacity to reduce the production of NO and ROS, as well as the levels of IL-1, IL-6, and TNF-α and the count of neutrophils. Therefore, it was suggested that EBNP accelerated wound healing by reducing inflammation, as well as enhancing ECM remodeling through EGF-like activity, including promoting the synthesis of collagen and elastin to quickly mend wounds. It could be concluded that the EBNP had the potential to promote tissue regeneration and skin repair in the fields of food, medicine and cosmetics.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comprehensive Review on Edible Packaging and Its Formation Methods With Recent Eco-Friendly Advancements","authors":"Ritika Negi,&nbsp;Shashikant Yadav","doi":"10.1002/bip.70053","DOIUrl":"10.1002/bip.70053","url":null,"abstract":"<div>\u0000 \u0000 <p>With the increase in human population and rising food demand, the need for an efficient and sustainable food packaging solution has intensified. This review discusses the edible and biodegradable packaging systems, emphasizing their potential as an environmentally safe and sustainable alternative to synthetic packaging. It also highlights various biopolymers, fabrication methods, and bioactive agents such as antimicrobials, antioxidants, and nanoparticles to improve the biopolymer's functionality, food preservation, and shelf-life extension. While edible and biodegradable packaging shows promising potential as sustainable packaging, further research is essential to optimize their formulations, cost-effectiveness, and improve scalability. The long-term safety of these packaging materials on human health, their industrial applicability, and interaction with food products still require thorough investigations.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poly(3-Hydroxybutyrate-Co-3-Hydroxyhexanoate): Real-Time Monitoring of Microbial Degradation via Quartz Crystal Microbalance and Electrochemical Measurement","authors":"Noriyuki Asakura,&nbsp;Takuma Otsuki,&nbsp;Momoko Kitamura,&nbsp;Tomohiro Hiraishi,&nbsp;Hideki Abe","doi":"10.1002/bip.70044","DOIUrl":"10.1002/bip.70044","url":null,"abstract":"<p>Poly(3-hydroxybutyrate-<i>co</i>-3-hydroxyhexanoate) (PHBH), produced by some bacteria, including <i>Aeromonas</i> strains, exhibits excellent environmental biodegradability, even in marine environments where biodegradation is typically poor. However, the exact mechanisms underlying this biodegradability remain to be elucidated. To evaluate the mechanisms of microbial degradation of PHBH, focusing on the initial stages, PHBH degradation by <i>Comamonas testosteroni</i> is analyzed, using a quartz crystal microbalance (QCM), cyclic voltammetry (CV), impedance, and scanning electrochemical microscopy (SECM). Real-time monitoring of bacterial adsorption followed by PHBH degradation is quantitatively achieved at the cellular level using a highly sensitive QCM. CV and impedance measurements suggest that microbial degradation of PHBH proceeds in a heterogeneous manner. The SECM observations reveal the heterogeneous microbial degradation of PHBH, which is highly consistent with the QCM, CV, and impedance measurements. These findings indicate that this analytical system, combined with highly sensitive QCM analysis and electrochemical measurement, is an effective tool for studying the microbial degradation of biodegradable plastics.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nisin-Incorporated Solid Lipid Nanoparticles-Based In Situ Ocular Gel Using Box–Behnken Design for Enhanced Antibacterial Activity: In Vitro-Ex Vivo-In Vivo Analysis","authors":"Meghanath B. Shete,&nbsp;Vaishnavi Fatangare,&nbsp;Sopan Nangare,&nbsp;Pankaj Jain,&nbsp;Shailesh S. Chalikwar","doi":"10.1002/bip.70052","DOIUrl":"10.1002/bip.70052","url":null,"abstract":"<div>\u0000 \u0000 <p>Microbial ocular infections, namely bacterial conjunctivitis (BC), are a major concern in the biomedical field. Nisin (NIS) is an amphiphilic natural antimicrobial peptide. It showed antibacterial potential against <i>Pseudomonas aeruginosa</i>, which is responsible for BC. Despite this, the application of NIS in pharmaceuticals for the treatment of ocular infections is hindered by several limitations that include poor aqueous solubility and stability. The preference for solid lipid nanoparticles (SLN) shows the aptitude to enhance solubility, bioavailability, etc., of therapeutically active molecules. Therefore, the present research work intends to prepare a thermoresponsive poloxamer 407 (P-407)-based in situ ocular gel of NIS-incorporated SLN using Box Behnken Design (BBD) for improved antibacterial application. Herein, NIS-SLN was formulated with glyceryl monostearate (GMS) and Tween 80 using a HSH-probe sonication method. It resulted in the spherical shape NIS-SLN with the particle size (PS) of 158.8 ± 13.56 nm, zeta potential (ZP) of −22.48 ± 1.86 mV, and drug loading (DL) of 12.8% ± 2.84%. The formulated thermo-responsive in situ gel (ISG) pH, gelling temperature, and viscosity were found to be 7.45 ± 0.02, 36.5°C ± 0.5°C, and 465.5 ± 6.5 cps, respectively, with drug release of 68.65% ± 5.1% over 24 h. Moreover, it shows improved permeation of 66.43% ± 2.6%, which might be because of the nanoscale dimensions of SLN and Tween 80. The formulation demonstrates good stability for 3 months and improved antimicrobial potential against <i>P. aeruginosa</i> compared to pure NIS, possibly owing to sustained release and improved penetration of NIS. Moreover, in vivo experiments demonstrated no irritation of the gel formulation, confirming biocompatibility with the ocular region. In conclusion, the SLN incorporated thermo-responsive P-407-based in situ ocular gel provides the improved potential of NIS. In the future, it will reveal a new horizon for the delivery of NIS and other molecules for ocular disease treatment.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the Rheological Properties and Printability of Sodium Alginate–Carboxymethyl Chitosan Composite Solutions for Tissue Scaffold Printing","authors":"Xavier L. Tabil,&nbsp;Tate N. Cao,&nbsp;Xiongbiao Chen","doi":"10.1002/bip.70050","DOIUrl":"10.1002/bip.70050","url":null,"abstract":"<p>Composites of sodium alginate (Alg) and carboxymethyl chitosan (CMCS) are used to 3D print tissue scaffolds, but the rheological properties and printability of these composites remain underreported, resulting in time-consuming trial-and-error printing. This study investigates these properties to rigorously design the 3D printing process. Dynamic shear tests characterize viscoelastic and frequency-dependent properties, while steady shear tests assess the apparent viscosity and temperature-dependent viscosity. A novel approach based on mass flow rate models guides the printing of two-layer scaffolds for printability analysis. Brightfield microscopy and printability indexes quantify the deviations between printed and designed scaffolds, defined as printability. Results show that Alg predominantly directs the rheological properties. At 4% w/v Alg, the addition of &lt; 3% w/v CMCS reduces elasticity, contrary to the trend where increasing CMCS increases elasticity. CMCS improves the thermal resistance of the composites, while Alg reduces it. Of the composites printed, a 4% w/v Alg + 1% w/v CMCS formulation most accurately replicates the designed scaffold, and adding CMCS improves scaffold printing repeatability by at least threefold compared to Alg-only solutions. These findings provide a framework that informs the preparation and performance of Alg-CMCS composites with tunable properties, advancing scaffold bioprinting for tissue engineering.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison of Ester- and Ether-Based Bio-TPUs With Regard to Their Resistance to Environmental Influences in Terms of Their Mechanical and Adhesive Properties","authors":"Marco Klute,&nbsp;Hans-Peter Heim","doi":"10.1002/bip.70051","DOIUrl":"10.1002/bip.70051","url":null,"abstract":"<p>The demand for bio-based alternatives to fossil-based plastics is growing rapidly due to the increasing environmental awareness of consumers and manufacturers, as well as the goal of carbon-neutral production. There are many promising alternatives that can be obtained from various renewable resources, but their use in series production, especially of technical components, is often hampered by doubts about their usability and, above all, their resistance to environmental influences. The present study is intended to help overcome these obstacles and demonstrate the applicability of bio-based TPUs in multi-component technical parts with high bonding requirements. Different polyester and polyether TPUs were used, and their resistance to elevated temperatures and humidity was compared. Both the mechanical properties and the bond strength in bio-based hard-soft composites were investigated. It was shown that good to very good bond strengths of approximately 2.5–8 N/mm could be achieved depending on the Shore hardness. The formation of adhesive forces depends on both the type of polyol and its proportion in the TPU. While ether-based TPU exhibited higher adhesive bond strengths, the strength increases with a higher proportion of soft segments. After storage tests, a decrease in bond strength was observed, mainly due to thermal aging effects and absorption of water molecules, correlating with the change in mechanical properties.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.70051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Biomaterial for Dual-Drug Release: A Hydrogel-Microparticle Approach","authors":"Jose Gregorio Fontainez Garrido,&nbsp;Newton Andreo Filho,&nbsp;Fabiana Perrechil,&nbsp;Mariana Agostini de Moraes","doi":"10.1002/bip.70049","DOIUrl":"https://doi.org/10.1002/bip.70049","url":null,"abstract":"<p>Advanced biomaterials with dual drug delivery represent a promising strategy to enhance therapeutic outcomes in wound treatment. This work aimed to combine antimicrobial and analgesic actions in a single platform, enabling the simultaneous release of both drugs from an advanced dual-drug delivery system based on a combined hydrogel and microparticle approach. The system was composed of alginate microparticles containing the antibiotic gentamicin incorporated into a gellan gum/collagen hydrogel matrix, in which the local anesthetic bupivacaine was directly loaded. The resulting composite was thoroughly characterized in terms of its morphological, physicochemical, mechanical, rheological, and thermal properties, as well as drug release profiles. The incorporation of microparticles significantly influenced the structural and functional behavior of the hydrogel, particularly at higher microparticle concentrations (50% w/v). Notably, the microparticles played a crucial role in maintaining the hydrogel's integrity in the presence of both drugs and enabled their controlled and simultaneous release, with each exhibiting distinct release kinetics. These findings highlight the potential of this hydrogel and microparticle composite as an advanced material for wound dressings, capable of promoting healing while simultaneously providing localized pain relief.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Chitosan@Magnetite Adsorbents for the Removal of Heavy Metals and Organic Pollutants From Water","authors":"Noura N. Eltahan,&nbsp;Hamada H. Abdel-Razik,&nbsp;Fathy M. ElTaweel,&nbsp;Magdy Y. Abdelaal","doi":"10.1002/bip.70047","DOIUrl":"10.1002/bip.70047","url":null,"abstract":"<div>\u0000 \u0000 <p>Chitosan@Magnetite composites have gained significant attention in the field of water purification due to their high capacity to absorb contaminants and their ease of separation using magnets. In this review, we reviewed the latest methods for preparing this material and explained its key properties, particularly its structural shape and the ways of modifying it to be more efficient. One of its most obvious advantages is the increase of surface area, which helps improve absorption. To this end, we mentioned several analytical techniques, such as morphology and structure, to understand how this material works. We also explained how this material can remove heavy metals and organic pollutants from water. Finally, we evaluated its performance in terms of absorption capacity, reaction rate, and its ability to be regenerated and reused, confirming its excellent suitability for practical and sustainable water purification.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability of Bioactive Compounds From Argemone platyceras in Sorbitol-Gum Arabic Biopolymer Films: A Food Coating Alternative","authors":"Daniel Arizmendi-Cotero,&nbsp;Rosa Maria Gómez-Espinosa","doi":"10.1002/bip.70048","DOIUrl":"https://doi.org/10.1002/bip.70048","url":null,"abstract":"<div>\u0000 \u0000 <p>A plastic film made from Gum Arabic and sorbitol (BioFilm-EAp) was developed to enhance the stability of bioactive compounds from <i>Argemone platyceras</i> (EAp) and preserve their antimicrobial properties. The EAp compounds identified through spectrophotometric methods in ethanolic extracts of leaves and stems included alkaloids (3320 and 1260 cm⁻¹), flavonoids (1739 cm⁻¹), and phenols (1260 cm⁻¹). Additionally, the extracts demonstrated the ability to inhibit the growth of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. The BioFilm, with and without EAp, was characterized through mechanical tests, revealing that films containing EAp were less resistant (1.07–11.82 N) than those without compounds (23.02 N). Furthermore, these properties depended on the concentration of sorbitol. The presence of alkaloids, flavonoids, and phenols in the BioFilm-EAp was assessed qualitatively using a simple and inexpensive methodology based on UV–Vis spectroscopy. The results indicated that these compounds remained stable within the sorbitol-Gum Arabic biopolymer matrix over 21 days. Finally, a sample of grapes was coated with the BioFilm-EAp films through the solution immersion method. This coating preserved the physical parameters of the grapes stored at room temperature, while the active compounds inhibited the growth of microorganisms on the grapes.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Nano ZnO-Embedded Gelatin/Alginate Bioscaffolds for Potential Skin Tissue Regeneration via Oxidative Stress Modulation and ECM Mimicry","authors":"Marija M. Babić Radić,&nbsp;Martina Žabčić,&nbsp;Marija Vukomanović,&nbsp;Jasmina Nikodinović-Runić,&nbsp;Dušan Milivojević,&nbsp;Vuk Filipović,&nbsp;Simonida Tomić","doi":"10.1002/bip.70046","DOIUrl":"https://doi.org/10.1002/bip.70046","url":null,"abstract":"<div>\u0000 \u0000 <p>Engineering of biomaterials for advanced skin tissue regeneration requires optimization of critical parameters including interconnected porous structure, biomaterial stability, hydrophilicity, biocompatibility, and bioactivity. These features enable the mimicry of the skin tissue microenvironment and support the key phases of the regeneration process, which are crucial for effective tissue repair. Another important requirement for successful skin tissue regeneration is the modulation of oxidative stress, as excessive accumulation of reactive oxygen species (ROS) at the site of the skin lesion can hinder healing and cause chronic inflammation and scarring. To address these challenges, we propose a reductionist therapeutic approach to skin tissue regeneration by developing bio-sourced scaffolds that replicate the native extracellular matrix (ECM), neutralize ROS levels, and actively promote tissue regeneration at both structural and molecular levels. These nano ZnO-embedded gelatin/alginate bioscaffolds were prepared via a simple crosslinking reaction and loaded with carefully selected active agents with antioxidant and skin tissue regenerative potential. Characterization studies of the bioscaffolds confirmed their porous interconnected morphology with tunable porosity (92%–94%), mechanical strength (1.95–3.22 MPa), hydrophilicity, stable adhesion to skin tissue, and ROS-scavenging activity. Additionally, the bioscaffolds demonstrated simultaneous release of quercetin, allantoin, and caffeic acid, and both biocompatibility—in vitro on human fibroblasts (MRC5) and in vivo on <i>Caenorhabditis elegans</i>. Overall, these findings provide valuable insight into the design of multifunctional bioscaffolds as a promising therapeutic platform for skin tissue regeneration application, which simultaneously modulates oxidative stress, replicates ECM architecture, and stimulates the healing cascade, ultimately enhancing skin tissue repair and reducing scarring.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}