Biopolymers最新文献

Advanced Drug Delivery Systems Utilizing β-Lactoglobulin: An Efficient Protein-Based Drug Carrier

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-02-06 DOI: 10.1002/bip.70005

Charan M. Gowda, Sanjay Sharma, Sarika Wairkar

{"title":"Advanced Drug Delivery Systems Utilizing β-Lactoglobulin: An Efficient Protein-Based Drug Carrier","authors":"Charan M. Gowda, Sanjay Sharma, Sarika Wairkar","doi":"10.1002/bip.70005","DOIUrl":"https://doi.org/10.1002/bip.70005","url":null,"abstract":"<div>\u0000 \u0000 <p>Proteins have shown significant potential as carrier systems due to specific binding interactions with several drug molecules. Among several other animal proteins, whey protein (WP) is a by-product of the dairy industry, mainly composed of globular proteins. β-Lactoglobulin (BLG) is a major component of WP, which offers a unique functional property for drug delivery, such as thermal stability, binding interactions, favorable charge characteristics, and a spherical shape. Several drug delivery systems (DDSs) have been developed using BLG as a carrier, including nanoparticles, nanocapsules, nanocomposites, nanoemulsions, solid dispersions, microparticles, and hydrogels. These delivery systems improve drug solubility, loading capacity, bioavailability, stability, and release rate and can provide targeted delivery. They have been employed in diverse applications, from treating cancer to enhancing oral drug delivery, reducing the toxicity of specific drugs, and offering controlled drug release. The future of BLG DDSs holds the promise of combination therapies, personalized medicine, and improved targeting precision. This review aims to discuss the role and utilization of BLG in several DDSs as a versatile carrier, revolutionizing the pharmaceutical industry. However, further research is expected to focus on optimizing degradation rates, enhancing biological compatibility, and addressing potential immune responses of BLG-based drug carriers.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Scale Structures, Functional Properties, and Applications of Starch Modified by Dry Heat Treatment

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-01-30 DOI: 10.1002/bip.70000

Gonzalo Velazquez, Guadalupe Mendez-Montealvo, Pamela C. Flores-Silva, Adrian Soler

{"title":"Multi-Scale Structures, Functional Properties, and Applications of Starch Modified by Dry Heat Treatment","authors":"Gonzalo Velazquez, Guadalupe Mendez-Montealvo, Pamela C. Flores-Silva, Adrian Soler","doi":"10.1002/bip.70000","DOIUrl":"10.1002/bip.70000","url":null,"abstract":"<div>\u0000 \u0000 <p>Dry heat treatment (DHT) is considered a green technology to modify starch structure and functionality since it does not generate effluents and avoids the use of chemical compounds, however, there is still no comprehensive understanding of the effects and mechanisms on the multi-scale structure and their relationship with functionality. This paper reviewed and analyzed the effects of DHT on multi-scale starch structures and functional properties, compared the performance of continuous and repeated DHT, discussed a mechanism of starch dry heating, and summarized the applications of dry-heated starches. DHT evaporates water, accelerates the movement of starch molecules, and breaks hydrogen bonds, which changes the multi-scale structure. In turn, structural modifications promoted by DHT affect the hydration properties, thermal stability, slowly digestible/resistant starch formation, and glycemic index. The multi-scale structure and functional changes after DHT are strongly affected by the starch botanical source and process conditions. This review contributes to understanding the starch DHT modification and establishes a theoretical basis for advancing DHT applications in the starch industry.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histidine Tags in Human Recombinant Alpha B-Crystallin (HSPB5) Proteins Are Detrimental for Zinc Binding Studies

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-01-29 DOI: 10.1002/bip.70003

Srabani Karmakar, K. P. Das

{"title":"Histidine Tags in Human Recombinant Alpha B-Crystallin (HSPB5) Proteins Are Detrimental for Zinc Binding Studies","authors":"Srabani Karmakar, K. P. Das","doi":"10.1002/bip.70003","DOIUrl":"10.1002/bip.70003","url":null,"abstract":"<div>\u0000 \u0000 <p>The stability of α-crystallin, the major protein of the mammalian eye lens and a molecular chaperone, is one of the most crucial factors for its survival and function. The chaperone-like activity and stability of α-crystallin dramatically increased in the presence of Zn<sup>2</sup>. Each subunit of α-crystallin could bind multiple zinc atoms through inter-subunit bridging and cause enhanced stability. Three histidines H104, H111, and H119 of recombinant human αB-crystallin (HSPB5) are found to be the Zn<sup>2+</sup> binding residues. In this article, we did site-directed mutagenesis of six histidine residues and made five-point mutants and a double mutant of αB-crystallin. We studied the effect of zinc on the chaperone function, surface hydrophobicity, and stability of the histidine mutants. We removed the histidine tag from H18A and H101V mutants and studied the stability and chaperone function in the presence and absence of zinc. H83 and H111 mutations showed similar enhancement in chaperone function like WT in the presence of Zn<sup>2+</sup>. Point mutants having his tags showed similar stability enhancement, but point mutant H18A without his tag showed less enhancement in stability in the presence of zinc. This indicates the significance of the presence of his tags in the study of zinc binding interaction with recombinant human αB-crystallin.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and Adsorption Properties of Sodium Trimetaphosphate Crosslinked Porous Corn Starch

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-01-28 DOI: 10.1002/bip.70004

Yuxin Liu, Qinghua Pan, Zesheng Liang, Jingqiao Li, Rulong Wu

{"title":"Preparation and Adsorption Properties of Sodium Trimetaphosphate Crosslinked Porous Corn Starch","authors":"Yuxin Liu, Qinghua Pan, Zesheng Liang, Jingqiao Li, Rulong Wu","doi":"10.1002/bip.70004","DOIUrl":"10.1002/bip.70004","url":null,"abstract":"<div>\u0000 \u0000 <p>The crosslinked porous corn starch was prepared by two steps: the native corn starch was hydrolyzed by α-amylase and glucoamylase, then the porous corn was crosslinked by sodium trimetaphosphate (STMP). The morphology and size of granules, spherulites, crystal type, molecular structure, swelling properties, thermal stability and adsorption properties of the crosslinked porous starch were investigated. The results indicated that a lot of holes formed in the porous starch, and the particle size of starch granules decreased. Under the cross-linking action of STMP, the porous starch particles are cross-linked and agglomerated together. The crystalline form of porous starch presents A + V type, and crystallinity increased after crosslinking. The crosslinked porous starches have higher short-range ordering comparing to the porous without crosslinked porous starch. The crosslinking degree, melting enthalpy and melting peak of starch increased with the increase of STMP content. The bulk density and the vibrated density of the porous starch increased after crosslinking. With the increase of the content of STMP, the water and oil absorption of porous starch increased and then decreased. The MB adsorption capacity of crosslinked porous starch has the maximum value with the STMP 20 wt% content. MB adsorption behavior of porous starch is more consistent with the pseudo-second-order kinetic model, and the equilibrium adsorption increased after crosslinking.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reversible Redox Controlled DNA Condensation by a Simple Noncanonical Dicationic Diphenylalanine Derivative

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-01-28 DOI: 10.1002/bip.70001

Ana M. Bernal-Martínez, César A. Angulo-Pachón, Francisco Galindo, Juan F. Miravet

引用次数: 0

Eco-Friendly Fabrication of FeS2 QD-Chitosan Biopolymer Composites: Green Synthetic Approach

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-01-25 DOI: 10.1002/bip.70002

Aditi Sharma, S. Tharuny, Tejasvi Pandey, Vivek Pandey

{"title":"Eco-Friendly Fabrication of FeS2 QD-Chitosan Biopolymer Composites: Green Synthetic Approach","authors":"Aditi Sharma, S. Tharuny, Tejasvi Pandey, Vivek Pandey","doi":"10.1002/bip.70002","DOIUrl":"10.1002/bip.70002","url":null,"abstract":"<div>\u0000 \u0000 <p>In this paper, we offer a unique green synthetic approach for producing iron sulfide quantum dots (FeS<sub>2</sub> QD)-chitosan composites using gel chemistry. The technique uses the environmental features of chitosan, a biocompatible and biodegradable polysaccharide, and the excellent electrical properties of FeS<sub>2</sub> QDs. By sustainable chemistry principles, the synthesis process is carried out under gentle settings, using aqueous solutions and avoiding hazardous solvents and strong chemicals. The resulting FeS<sub>2</sub> QD-chitosan composite has superior structural integrity, homogeneous QD distribution, and improved physicochemical characteristics. Comprehensive characterization techniques, such as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and photoluminescence spectroscopy, confirm the successful integration of FeS<sub>2</sub> QDs into the chitosan matrix while preserving their quantum properties. This work demonstrates the viability of gel chemistry as a green synthetic technique for generating functional nanocomposites, providing a scalable and environmentally responsible option for advanced material development.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating Electrospun Polycaprolactone Fibers for Blood-Contacting Applications 评价静电纺聚己内酯纤维的血液接触应用。

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-01-22 DOI: 10.1002/bip.23656

Muzammil Kuddushi, Aishwarya S Pawar, Mehdi Ghaffari Sharaf, Larry D. Unsworth, Xuehua Zhang

{"title":"Evaluating Electrospun Polycaprolactone Fibers for Blood-Contacting Applications","authors":"Muzammil Kuddushi, Aishwarya S Pawar, Mehdi Ghaffari Sharaf, Larry D. Unsworth, Xuehua Zhang","doi":"10.1002/bip.23656","DOIUrl":"10.1002/bip.23656","url":null,"abstract":"<div>\u0000 \u0000 <p>When the kidneys are injured, uremic toxins (UTXs) accumulate in the body, affecting other tissues and causing a loss of essential body functions. This study investigated the adsorption of blood plasma-laden UTXs on the surface of PCL fibers to assess their potential as an alternative to membrane dialysis materials. Using plasma containing 26 UTXs at a concentration similar to that found in end-stage kidney disease patients, we analyzed the adsorbed proteins and examined clot formation in normal and toxin-treated plasma in the presence of PCL fibers. Our findings revealed that the presence of UTXs significantly increased the adsorption of proteins on PCL fiber meshes, without leading to increased clot formation. This suggests a lack of enzymatic activation despite the higher protein adsorption. Additionally, our study indicates that unmodified PCL surfaces have the potential to trigger a strong humoral immune response, underscoring the importance of understanding these interactions for the development of personalized treatment approaches for patients with kidney failure.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma-Activated Water/Ultrasound as a Green Method to Modify Wood Fiber By-Product: Insights of Their Mechanical Performance in Polylactic Acid-Based Biofilms 等离子活化水/超声作为一种绿色方法来修饰木纤维副产品：它们在聚乳酸基生物膜中的机械性能的见解。

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-01-17 DOI: 10.1002/bip.23655

Alexandra Trejo-Zuñiga, Pamela C. Flores-Silva, Ernesto Hernandez-Hernandez, Guadalupe Neira-Velazquez, José F. Hernandez-Gamez, Guadalupe Mendez-Padilla, Esmeralda Saucedo-Salazar, Israel Sifuentes-Nieves

引用次数: 0

Molecular Weight-Dependent Boron Release Effect in PVA/Chitosan Cryogels and In Vitro Mineralization Evaluations by Osteoblast Cells 分子量依赖性硼在聚乙烯醇/壳聚糖冰柜中的释放效应及成骨细胞体外矿化评价。

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-01-10 DOI: 10.1002/bip.23654

Seda Ceylan, Şule Arıcı, Duygu Ege, Ying Yang

{"title":"Molecular Weight-Dependent Boron Release Effect in PVA/Chitosan Cryogels and In Vitro Mineralization Evaluations by Osteoblast Cells","authors":"Seda Ceylan, Şule Arıcı, Duygu Ege, Ying Yang","doi":"10.1002/bip.23654","DOIUrl":"10.1002/bip.23654","url":null,"abstract":"<div>\u0000 \u0000 <p>Cryogels were fabricated by combining polyvinyl alcohol (PVA) and chitosan of varying molecular weights (Mw). In this study, the effects of chitosan Mw, types of boron-containing molecules on network formation, and boron release rate in resulted cryogels were investigated. The PVA/chitosan blend maintained a constant 4.5% (w/v) polymer content. PVA to chitosan weight ratio of 6:1 was maintained and fixed. Five percent w/w boric acid and borax (sodium tetraborate) crosslinkers were added in PVA and chitosan mixtures to construct cryogels. The freeze-dried specimens underwent crosslinking evaluation, chemical composition analysis by FTIR, and boron release studies by ICP-MS. The pore morphology and the swelling capacity of the cryogel have been assessed by SEM and incubation in water, respectively. Mechanical test was also used to evaluate the effect of borax and Mw of chitosan on cryogels' mechanical properties. It was demonstrated that the types of boron supply had a significant role on the cryogelation capability. For different chitosan Mw, the cryogels made using borax showed stable cryogels. In contrast, even after altering the chitosan Mw, the formula with boric acid was unable to create stable cryogels. In addition, boron release assay showed that the quantity of free boron in the incubation solutions decreased as the Mw of the chitosan component of the cryogel was reduced. Cell culture studies with MC3T3-E1 pre-osteoblast cells in the cryogels indicated that borax-crosslinked samples exhibited sustained cell viability. Alizarin red staining assay was used to study mineralization capacity of boron-containing hydrogels, which confirmed increase of mineralization in low molecular weight chitosan groups.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Flexible Methyl Cellulose/Gelatin Hydrogels for Potential Cartilage Tissue Engineering Applications 高柔性甲基纤维素/明胶水凝胶的潜在软骨组织工程应用。

IF 3.2 4区生物学

Biopolymers Pub Date : 2025-01-07 DOI: 10.1002/bip.23641

Mehmet Ali Karaca, Vida Khalili, Duygu Ege

{"title":"Highly Flexible Methyl Cellulose/Gelatin Hydrogels for Potential Cartilage Tissue Engineering Applications","authors":"Mehmet Ali Karaca, Vida Khalili, Duygu Ege","doi":"10.1002/bip.23641","DOIUrl":"10.1002/bip.23641","url":null,"abstract":"<p>Cartilage damage resulting from trauma demonstrates a poor capacity for repair due to its avascular nature. Cartilage tissue engineering offers a unique therapeutic option for cartilage recovery. In this study, methylcellulose (MC)/gelatin (GEL) hydrogels (MC10G20, MC12.5G20, MC15G20, and MC17.5G20) were developed to assess and compare their chemical, mechanical, and biological characteristics for cartilage repair. First, the interaction between MC and GEL after blending and subsequent crosslinking with EDC/NHS was confirmed by using FTIR. Mechanical tests under compression test revealed that hydrogels' resistance to both elastic and plastic deformation increased with higher wt.% of MC. The % strain of the hydrogels doubled with the addition of MC, likely due to abundant hydrogen bonding between polymeric chains. Furthermore, the compressive modulus of MC/GEL hydrogels was approximately 0.2 MPa, closely matching modulus of human cartilage tissue. Similarly, the % water retention capacity of the hydrogels increased over the 7 days as the MC content increased. Additionally, SEM images showed that the incorporation of MC to GEL introduced porosity with the diameters ranging from 10 to 50 μm, similar to the size of pores in native cartilage. In vitro cell culture studies confirmed the biocompatibility of MC/GEL hydrogels. Fluorescence staining showed a 2.5-fold increase in F-actin staining following the incorporation of MC into the hydrogels. Overall, this study highlights the potential of MC/GEL hydrogels for cartilage tissue engineering, however, further research is required to assess its full potential.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0