Biopolymers最新文献

{"title":"Zeolite-Reinforced PVA/Chitosan/Hyaluronic Acid Composite Films as Biocompatible Tissue Patches","authors":"Hasan Hüseyin Gülercan,&nbsp;Seda Genç Şimşek,&nbsp;Kadriye Kızılbey,&nbsp;Cenk Denktaş,&nbsp;Nelisa Türkoğlu","doi":"10.1002/bip.70116","DOIUrl":"10.1002/bip.70116","url":null,"abstract":"<div>\u0000 \u0000 <p>Tissue patches are biomaterial-based structures designed to support the repair of damaged or functionally impaired tissues and are required to exhibit biocompatibility, mechanical integrity, and suitable surface characteristics. In this study, poly(vinyl alcohol) (PVA), chitosan (Chi), and hyaluronic acid (HA)-based composite films reinforced with zeolite (0–0.5% w/v) were developed and evaluated as potential tissue patch materials. The incorporation of zeolite significantly influenced the physicochemical and mechanical properties of the films. The elastic modulus decreased from 293.78 ± 64.47 N/mm² for the zeolite-free film to 106.21 ± 9.50 N/mm² at the highest zeolite content, indicating tunable flexibility. Water contact angle values increased from 46.09° to 67.23°, while maintaining overall hydrophilicity. The films exhibited rapid swelling behavior, reaching equilibrium within 30 min, and demonstrated controlled biodegradation with mass losses exceeding 75% after 42 days. Biological evaluations showed that all formulations maintained cell viability above 70%, with values ranging from 87.26% to 78.63%, and supported cell adhesion, confirming their biocompatible nature. The novelty of this study lies in demonstrating that low-concentration zeolite incorporation enables controlled tuning of mechanical, surface, and biological properties within a single PVA–Chi–HA system, without compromising biocompatibility. These findings highlight the potential of zeolite-reinforced composite films as multifunctional and customizable tissue patch candidates for tissue engineering applications.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 5","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Processing of Sugar Beet Pulp: Pectin and Microcrystalline Cellulose Production and Their Properties","authors":"S. T. Minzanova,&nbsp;Y. V. Chekunkov,&nbsp;A. V. Khabibullina,&nbsp;L. G. Mironova,&nbsp;A. R. Khamatgalimov,&nbsp;A. T. Gubaidullin,&nbsp;G. R. Nizameeva,&nbsp;V. A. Milyukov","doi":"10.1002/bip.70115","DOIUrl":"10.1002/bip.70115","url":null,"abstract":"<div>\u0000 \u0000 <p>Sugar beet pulp is a multi-ton waste product of the sugar beet industry. In this study, using chemical processing, beet pectin and microcrystalline cellulose were sequentially obtained from sugar beet pulp by acid extraction and alkali treatment, bleaching, and sulfuric acid hydrolysis, respectively. The resulting biopolymers were characterized using infrared spectroscopy (IR spectroscopy), X-ray diffraction (XRD), atomic force microscopy (AFM), and thermogravimetric analysis (TGA) to determine their chemical structure, morphology, thermal stability, and the crystallinity of the microcrystalline cellulose. Physicochemical analysis showed that the isolated pectin is characterized by a high molecular weight (39.25 kDa) and degree of esterification (75.0%). The crystallinity index of the obtained cellulose, calculated using XRD, is 86.4%. The yield of pectin on the absolute dry weight (ADW) basis was 14.5% by mass, and the yield of microcrystalline cellulose on the ADW basis was 34.5% by mass. Using the TGA/DSC method, it was determined that the thermal degradation temperature of pectin is 257.4°C, and that of microcrystalline cellulose is 344.4°C. The valuable biocompatible biopolymers obtained are in demand in the food and pharmaceutical industries, as well as in medicine and cosmetology.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methionine Oxidation Stabilizes α-Helices but Attenuates Biological Activity in Amphipathic Peptides","authors":"Manami Fuchinoue,&nbsp;Kiyohiko Seki,&nbsp;Kaoru Araki,&nbsp;Tomoichirou Kusumoto,&nbsp;Junichi Taira,&nbsp;Satoshi Osada","doi":"10.1002/bip.70117","DOIUrl":"10.1002/bip.70117","url":null,"abstract":"<div>\u0000 \u0000 <p>The relationship between α-helicity and membrane activity is a central yet unresolved issue in the design of membrane-active peptides. While the amphipathic α-helical structure is widely regarded as a key determinant of antimicrobial activity, increasing evidence suggests that enhanced helicity does not necessarily translate into effective membrane disruption. Here, we investigate this structure–function relationship using a well-defined synthetic amphipathic α-helical scaffold, BKBA-20, to which methionine residues were introduced at specific locations and compared in their reduced and oxidized (methionine sulfoxide) forms. Methionine oxidation provides a unique means to modulate side-chain polarity without altering the peptide backbone, enabling experimental decoupling of α-helical stability from hydrophobic driving forces. Circular dichroism analyses revealed that oxidation to methionine sulfoxide increased α-helical content in aqueous buffer. Strikingly, however, oxidized peptides exhibited markedly attenuated antimicrobial and hemolytic activities, regardless of their sequence. In addition, the biological activities of the reduced peptides depended strongly on methionine placement, resulting in a clear positional bifurcation between antimicrobial-selective and variants exhibiting relatively higher, but still modest, hemolytic activity. These findings demonstrate that α-helicity is necessary but not sufficient for biological activity and that productive biological function is governed by overall hydrophobicity rather than secondary structure alone. The observed decoupling of helicity from biological activity is consistent with conceptual frameworks such as the interfacial activity model and highlights methionine oxidation as a molecular switch for tuning structure–function relationships. This work provides mechanistic insight into functional selectivity and offers design principles for redox-responsive biologically active peptides.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untreated Rosehip Powder/Poly(Lactic Acid)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Electrospun Mats for Wound Dressing Applications","authors":"Naciye Akman,&nbsp;Sema Samatya Yilmaz,&nbsp;Ikrime Orkan Ucar,&nbsp;Huseyin Uzuner,&nbsp;Ayse Aytac","doi":"10.1002/bip.70114","DOIUrl":"10.1002/bip.70114","url":null,"abstract":"<p>This study investigated the effects of directly incorporating untreated rosehip (RoHi) powder into electrospun poly(lactic acid)/poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (PLA/P(3HB-co-4HB)) (70/30, w/w) blends, focusing on the structural, morphological, mechanical, wettability, and biological properties of resulting nanofibrous mats. The average fiber diameter of pure nanofibers was 776 nm, while RoHi addition resulted in diameters ranging from 468 nm (1% RoHi) to 1496 nm (5% RoHi). FTIR analysis confirmed that the characteristic functional groups of RoHi were preserved within the polymer matrix without chemical interaction. The water contact angle decreased from 136.45° for the pure mat to 116.11° for nanofibers containing 5% RoHi, leading to an increase in liquid adsorption capacity from 1.49% to 249.38%. Cytotoxicity results demonstrated that all samples were biocompatible, with the highest cell viability (148.91%) observed for nanofibers containing 1% RoHi. In vitro scratch assays revealed enhanced fibroblast migration for RoHi-containing nanofibers, particularly within the first 24 h, with the fastest wound closure observed at 3% and 5% RoHi contents. DPPH analysis showed increased antioxidant activity from 27.68% to 63.84%, whereas no antibacterial activity was detected against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>. Overall, RoHi-doped nanofibers exhibit promising potential as wound dressing materials considered suitable for use in chronic wounds due to their antioxidant activity and bio-responsive wound-healing performance.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.70114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148381707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, Characterization, and Antidiabetic Evaluation of Sequence-Modified Liraglutide Analogs in a Drosophila melanogaster Model","authors":"Tamira Ravi,&nbsp;Kalpanarani Dash,&nbsp;Deep Kumar Barman,&nbsp;Hyukjin Kwon,&nbsp;Monalisa Mishra,&nbsp;Harekrushna Sahoo","doi":"10.1002/bip.70113","DOIUrl":"10.1002/bip.70113","url":null,"abstract":"<div>\u0000 \u0000 <p>Liraglutide, a glucagon-like peptide-1 receptor agonist, is widely used as a therapeutic macromolecule for the treatment of type 2 diabetes; however, its large-scale production is limited by high manufacturing costs and the frequent occurrence of closely related deletion impurities during synthesis. In the present study, we describe a novel, impurity-controlled, and industrially feasible synthetic strategy for liraglutide and its sequence-modified analogs. This method utilizes solution-phase incorporation of Pal-γ-Glu-OtBu in combination with a preassembled Boc-His (Boc)-Ala-Glu (OtBu)-OH tripeptide fragment, effectively minimizing the formation of des-His, des-Ala, and des-Glu impurities. The optimized protocol enabled the production of liraglutide and its analogs with consistent isolated yields and high chromatographic purity (&gt; 95% by RP-HPLC), suitable for subsequent biological evaluation. The antidiabetic potential of liraglutide and three sequence-modified analogs, Lira (Trp-O²⁵), Lira (desGly³¹), and Lira (Glu¹⁷), was evaluated using a <i>Drosophila melanogaster</i> model of high-sucrose diet induced diabetes. Among these, Lira (Glu¹⁷) exhibited the most pronounced metabolic improvements, significantly reducing free glucose (<i>p</i> &lt; 0.001), trehalose (<i>p</i> &lt; 0.001), and triglyceride levels (<i>p</i> &lt; 0.001) when compared to diabetic controls. Furthermore, this analog effectively decreased lipid accumulation and reactive oxygen species in larval gut tissues and enhanced locomotor performance in both larva and adult flies. While Lira (Trp-O²⁵) also demonstrated beneficial effects, Lira (desGly³¹) showed comparatively limited efficacy. Collectively, this study presents a cost-effective and impurity-controlled synthetic platform for liraglutide production and identifies Lira (Glu¹⁷) as a promising analog with enhanced antidiabetic activity, offering valuable insights for peptide manufacturing and GLP-1-based therapeutic development.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic Grafting of Oxidized Cellulose Nanofibers Using Ferulic Acid: Structural and Antioxidant Analysis Toward Bioactive Nanomaterials.","authors":"Mayra A Mariño, Cécile Sillard, Yesid Javier Rueda-Ordóñez, Alain Dufresne, Daniel A Palacio, Johanna Castaño","doi":"10.1002/bip.70111","DOIUrl":"10.1002/bip.70111","url":null,"abstract":"<p><p>Incorporating ferulic acid (FA) into a polymeric hydrogel impairs its bioactive properties. The synthesis used in this study involved radical-mediated surface grafting of nanocellulose for 24 h, using 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) to oxidize cellulose nanofibers (oCNF) and phosphorylated cellulose nanofibers (pCNF), resulting in total polyphenol contents of 30.6 and 6.3 mg/g, respectively. Spectroscopic analyses of oCNF included solid-state high-power decoupling with magic-angle spinning nuclear magnetic resonance (¹³C HPDEC/MAS NMR), Fourier transform infrared (FTIR) spectroscopy, and X-ray photoelectron spectroscopy (XPS), confirming that FA was successfully grafted onto the backbone through ester linkages on the nanofibril surface. Rheology testing and morphological analysis via transmission electron microscopy (TEM) further supported these findings. TEM imaging revealed that the grafting process preserved the nanoscale morphology, yielding fibrils with an average diameter of approximately 28.2 ± 9.3 nm. Key aspects affecting FA grafting include the colloidal stability of the CNF suspension, evaluated by zeta potential, and surface activation via oxidation. In this study, FA was grafted onto oCNF through a radical-induced reaction to enhance their functional performance, enabling the development of functional and sustainable hydrogels with high antioxidant capacity (85.62% ± 1.65%).</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 4","pages":"e70111"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147980316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin Probe via Disulfide Binding.","authors":"Yutaka Ouchi, Koki Ishii, Yumiko Sato, Yoshitane Imai, Hideo Matsui, Takashi Ohtsuki, Yoshiyuki Hakata, Hajime Shigeto, Shohei Yamamura, Mizuki Kitamatsu","doi":"10.1002/bip.70105","DOIUrl":"10.1002/bip.70105","url":null,"abstract":"<p><p>The development of methods for detecting specific nucleic acids is important for early diagnosis and treatment of diseases at the genetic level. We have developed a pair of pyrene (Pyr)-modified peptide nucleic acids (PNAs), PNA twin probe, as a tool for such detection. In this study, we prepared Pyr-PNAs containing chloroacetyl (-COCH₂Cl) or thiol (-SH) groups at the termini by solid-phase peptide synthesis. By analyzing various candidates, we clarified that a pair of Pyr-PNAs, each containing an SH group, formed a disulfide bond through the hybrid formation of two PNAs with complementary DNA, resulting in excimer emission at 455 nm. Furthermore, we demonstrated that these Pyr-PNAs provide fluorescent detection of intracellular target RNAs through enhanced excimer emission via the ligation. This work should aid future studies aimed at the specific fluorescent detection of RNA in living cells.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 4","pages":"e70105"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13178207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147947753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Chitosan-Alginate Polyelectrolyte Complex Formation and Multilayer Polymer Configuration on the Characteristics of 3D-Printed Metronidazole-Loaded Periodontal Films.","authors":"Dicle Denizhan, Ayse Nur Buke, Muge Kilicarslan, Kaan Orhan, Hilal Basak Erol, Banu Kaskatepe","doi":"10.1002/bip.70107","DOIUrl":"10.1002/bip.70107","url":null,"abstract":"<p><p>This study aimed to prepare six different metronidazole-loaded film formulations by 3D printing for periodontal treatment. Chitosan (CH) and alginate (ALG) gels were prepared separately and printed layer-by-layer, varying polymer type and number of layers. Polyelectrolyte complex (PEC) gels were prepared by mixing CH and ALG (1:1) at different polymer concentrations to investigate the effect of complexation strength on film performance. Films were characterized by thickness measurements, drug content analysis, Micro-CT and SEM imaging, swelling, adhesion, antimicrobial activity, release kinetics, and cumulative drug release. Increasing ALG layers significantly enhanced swelling and accelerated metronidazole release, whereas CH-dominant films exhibited lower swelling and slower release. Overall, PEC films modified the drug release profile. Layer-by-layer printing promoted more uniform diffusion, whereas stronger polyelectrolyte complexation slowed drug release due to increased structural irregularity. All films were confirmed to exhibit microbiological activity. Micro-CT analysis in conjunction with SEM enabled precise evaluation of the layered film structure produced by 3D printing and detailed assessment of layer definition, internal heterogeneity, and their relationship with swelling and drug release behaviour. These findings suggest that tailoring the structural design via polymer layering or complexation represents a promising strategy for local drug delivery systems in periodontal therapy.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 4","pages":"e70107"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147986464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of an Antibacterial Alginate/Chitosan Hydrogel Dressing Loaded With CuO Nanoparticles for Wound Dressing Applications.","authors":"Bushra Mushtaq, Salman Bashir, Sumera Shaheen, Farooq Azam, Faheem Ahmad, Abher Rasheed, Sheraz Ahmad, Muhammad Sohail Zafar, Zulfiqar Ahmad Rehan","doi":"10.1002/bip.70112","DOIUrl":"10.1002/bip.70112","url":null,"abstract":"<p><p>Chronic wounds require multifunctional dressings capable of simultaneously controlling infection, managing exudate, and promoting tissue regeneration. This study develops CuO nanoparticles incorporated alginate/chitosan hydrogel dressing reinforced onto nonwoven fabrics of pure cotton, pure hemp, and 50:50 cotton: hemp blend fabricated via full factorial design for wound dressing applications. The composites were characterized by surface morphology, chemical structure, mechanical properties, antibacterial activity, air permeability, and water absorbency. Morphological analysis confirms uniform hydrogel coating and successful CuO deposition. Mechanical strength and water absorbency increased with hydrogel concentration, with a maximum tensile strength of 115.61 N for the hemp reinforced sample at 2%. All CuO-loaded hydrogel composite dressings exhibited effective antibacterial performance. Although higher hydrogel concentrations reduce air permeability, blended substrates achieve a balanced combination of strength, breathability, and exudate management. This eco-friendly, cost-effective composite demonstrates strong potential for infection control for wound healing applications.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 4","pages":"e70112"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148026402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148095666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}