Biopolymers最新文献_第2页

Effect of Different Ratios of Polyvinyl Alcohol-Gum Odina for the Preparation and Characterization of Biodegradable Composite Films. 不同比例的聚乙烯醇-奥迪纳胶对制备可生物降解复合膜及其特性的影响

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-09-23 DOI: 10.1002/bip.23630

Mousumi Tudu, Ahana Hazra, Pankaj Paul, Abhishek Mohanta, Sohini Chatterjee, Amalesh Samanta

{"title":"Effect of Different Ratios of Polyvinyl Alcohol-Gum Odina for the Preparation and Characterization of Biodegradable Composite Films.","authors":"Mousumi Tudu, Ahana Hazra, Pankaj Paul, Abhishek Mohanta, Sohini Chatterjee, Amalesh Samanta","doi":"10.1002/bip.23630","DOIUrl":"https://doi.org/10.1002/bip.23630","url":null,"abstract":"This research investigates the production of biodegradable films using a combination of gum odina (GO) and polyvinyl alcohol (PVA) with varied ratio. The study focuses on the chemical, physical, and mechanical properties of PVA-GO composite films, emphasizing how versatile and biodegradable they may be for a range of packaging applications. Solvent-cast PVA-GO films with different ratios are subjected to a methodical analytical process to determine several parameters like mechanical qualities, thermal stability, biodegradability in soil, contact angle, transparency, water vapor permeability, moisture content, thickness, density, water solubility, microstructure, and FTIR analysis. The outcomes demonstrate that GO improves UV barrier qualities and water vapor permeability. Additionally, the films showed notable biodegradability, acceptable thermal stability, and mechanical qualities. In short, PVA-GO films can provide an eco-friendly packing substitute with adaptable qualities fit for a range of uses. Therefore, this research may further contribute promising information in the field of biodegradable packaging materials in the future.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":" ","pages":"e23630"},"PeriodicalIF":3.2,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142280051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of In Situ-Cross-Linked N-Succinyl Chitosan/Oxidized Alginate Hydrogel-Loaded Ascorbic Acid and Biphasic Calcium Phosphate for Bone Tissue Engineering 用于骨组织工程的原位交联 N-琥珀酰壳聚糖/氧化海藻酸水凝胶负载抗坏血酸和双相磷酸钙的制备方法

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-09-20 DOI: 10.1002/bip.23628

Thanh-Thuong Le Duong, Binh Thanh Vu, Hanh Thi-Kieu Ta, Quan Minh Vo, Thanh Dinh Le, Thi-Hiep Nguyen

{"title":"Fabrication of In Situ-Cross-Linked N-Succinyl Chitosan/Oxidized Alginate Hydrogel-Loaded Ascorbic Acid and Biphasic Calcium Phosphate for Bone Tissue Engineering","authors":"Thanh-Thuong Le Duong, Binh Thanh Vu, Hanh Thi-Kieu Ta, Quan Minh Vo, Thanh Dinh Le, Thi-Hiep Nguyen","doi":"10.1002/bip.23628","DOIUrl":"https://doi.org/10.1002/bip.23628","url":null,"abstract":"Bone tissue engineering is a promising technology being studied globally to become an effective and sustainable method to treat the problems of damaged or diseased bones. In this work, we developed an in situ cross-linking hydrogel system that combined N-succinyl chitosan (NSC) and oxidized alginate (OA) at varying mixing ratios through Schiff base cross-linking. The hydrogel system also contains biphasic calcium phosphate (BCP) and ascorbic acid (AA), which could enhance biological characteristics and accelerate bone repair. The hydrogels' properties were examined through physicochemical tests such as scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FT-IR), x-ray diffraction (XRD), pore size and porosity measurement, swelling ratio, degradation rate, AA release study, as well as cytocompatibility, including live/dead and cytotoxicity assays. The results revealed that the supplementation of AA and BCP components can affect the physico-mechanical properties of the hydrogel system. However, they exhibited noncytotoxic properties. Overall, the results demonstrated that the hydrogel composed of 3% (w/v) NSC and 3% (w/v) OA (NSC: OA volume ratio is 8:2) loaded with 40% (w/w) BCP and 0.3 mg/mL AA has the potential for bone regeneration.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"40 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Basis for Antagonist Binding to Vasopressin V1b Receptor Revealed by the Molecular Dynamics Simulations 分子动力学模拟揭示血管加压素 V1b 受体拮抗剂结合的结构基础

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-09-17 DOI: 10.1002/bip.23627

Magdalena J. Ślusarz

{"title":"Structural Basis for Antagonist Binding to Vasopressin V1b Receptor Revealed by the Molecular Dynamics Simulations","authors":"Magdalena J. Ślusarz","doi":"10.1002/bip.23627","DOIUrl":"https://doi.org/10.1002/bip.23627","url":null,"abstract":"The human V1b receptor (V1bR) is primarily expressed in the corticotropic cells of the anterior pituitary where it is involved in the regulation of the hypothalamic–pituitary–adrenal (HPA) axis. The activation of V1bR induces the secretion of adrenocorticotropin hormone (ACTH) from the anterior pituitary cells which, in turn, stimulates the production of cortisol via the adrenal cortex. Clinical studies have demonstrated the chronic dysfunction of the HPA axis in patients with several psychiatric disorders. Thus, the inhibition of the V1b receptor and normalizing the HPA axis hyperactivity is a promising approach to the treatment of many stress-related disorders such as anxiety and depression. Nelivaptan is a selective V1bR antagonist that can be used for this purpose and an excellent molecule to study how antagonists interact with V1bR, especially since in recent years the experimental structures of vasopressin V2 and oxytocin receptors were solved, providing high-similarity templates for homology modeling of V1bR. Therefore, in this work, six independent molecular dynamics simulations of a V1bR-nelivaptan complex in a fully hydrated lipid bilayer, yielding a total simulation time of 6.0 μs, have been conducted. In the lowest-energy complexes obtained in this work and proposed to be the most probable structure of the V1bR-nelivaptan complex, the location of the ligand inside the receptor pocket is very similar to that of the other ligands observed in the experimental structures of the vasopressin/oxytocin receptor family. The receptor-ligand interaction has been analyzed and described, revealing the details of the molecular mechanism of this antagonist binding to V1bR and a probable contribution of L2005×40 and T2035×43 to binding selectivity.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"3 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autoclaved Starch: Structure and Functionality Relationship in a Matrix With the Same Contribution of Amylose and Amylopectin 高压灭菌淀粉：直链淀粉和支链淀粉含量相同的基质中的结构和功能关系

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-09-11 DOI: 10.1002/bip.23624

Gonzalo Velazquez, Guadalupe Mendez‐Montealvo, Eduardo Morales‐Sanchez, Israel Sifuentes‐Nieves, Rodrigo Velazquez‐Castillo, Adrian Soler

{"title":"Autoclaved Starch: Structure and Functionality Relationship in a Matrix With the Same Contribution of Amylose and Amylopectin","authors":"Gonzalo Velazquez, Guadalupe Mendez‐Montealvo, Eduardo Morales‐Sanchez, Israel Sifuentes‐Nieves, Rodrigo Velazquez‐Castillo, Adrian Soler","doi":"10.1002/bip.23624","DOIUrl":"https://doi.org/10.1002/bip.23624","url":null,"abstract":"The rational use of autoclaved starches in food applications is difficult because there is a lack of information on their structure–functionality relationship. The novelty of this research relies on disclosing such an association. Hylon V starch was autoclaved at 105, 120, and 135°C to investigate its crystalline and double‐helical features and its relationship with functionality. In autoclaved Hylon V starch, interactions of amylopectin and amylose improved while the crystalline regions decreased. The degree of double helices (DD) decreased after autoclaving at 105°C and the degree of order (DO) increased after treatment at 120 and 135°C. The water solubility index (WSI) (4.63–6.38%) and swelling power (SP) (4.39–7.1 g/g) increased when the temperature increased. On the other hand, water (103.49–225.01%) and oil (61.91–94.53%) holding capacity (WHC and OHC, respectively) increased after autoclaving treatment, although the values decreased with the treatment intensity. The functional properties were affected when the structure changed as a function of the treatment temperatures. PCA analysis showed that WSI and SP of autoclaved Hylon V starch were associated with a high DD, with better compaction, and with stronger amylopectin–amylose interactions. WHC and OHC were associated with better crystallinity, stronger interactions of amylopectin and amylose, and heterogeneous double‐helical crystallites. These findings are useful for understanding the structure–functionality relationship of autoclaved Hylon V starch and pave the way for future research regarding the effects of its incorporation on the properties of food matrices such as bread, yogurt, cakes, and pudding.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"109 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142195211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Tetrapropylammonium Chloride Quaternary Ammonium Salt on Characterization, Cytotoxicity, and Antibacterial Properties of PLA/PEG Electrospun Mat 四丙基氯化铵季铵盐对聚乳酸/聚乙二醇电纺丝毡的特性、细胞毒性和抗菌性能的影响

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-09-11 DOI: 10.1002/bip.23626

Sena Özdil Şener, Sema Samatya Yilmaz, Merve Dandan Doganci, Erdinc Doganci

{"title":"Effect of Tetrapropylammonium Chloride Quaternary Ammonium Salt on Characterization, Cytotoxicity, and Antibacterial Properties of PLA/PEG Electrospun Mat","authors":"Sena Özdil Şener, Sema Samatya Yilmaz, Merve Dandan Doganci, Erdinc Doganci","doi":"10.1002/bip.23626","DOIUrl":"https://doi.org/10.1002/bip.23626","url":null,"abstract":"In this study, poly(lactic acid) (PLA)–tetrapropylammonium chloride (TCL)–poly(ethylene glycol) (PEG) nonwoven networks were produced using PLA, PEG with different concentrations (3, 5, 7, and 9 wt%), and TCL. PEG is included as a plasticizer in PLA polymer, which has high biocompatibility but a brittle structure. The importance of this study is to investigate the effect of TCL salt on the characterization of PLA–PEG nanofibers. For this research, the cytotoxicity test system responsible for the fibroblast cell line (L929) was evaluated with the liquid absorption capacity (LAC) and drying time tests for its use in wound dressings. The addition of TCL salt reduced bead formation in PLA–PEG nanofibers and increased the homogeneity of fiber dispersion. The smoothest and most homogeneous nonwoven networks were obtained as PLA–5TCL–PEG. It was also reported that this nonwoven network exhibited liquid absorption behavior with a maximum increase of 150% compared to the PLA–PEG nonwoven network and had the highest Young's modulus value of 12.97 MPa. In addition to these tests, evaluations were made with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), drying time test, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and mechanical tests. In addition, high cell viability was observed in L292 mouse fibroblast cells at the end of the 24th hour, again with the effect of TCL salt. In addition, antibacterial activity was tested against gram‐negative E. coli and gram‐positive S. aureus bacteria, and it was observed that there was no antibacterial activity. Since PLA–TCL–PEG nonwoven webs have a maximum cell viability of 133.27%, they are recommended as a potential dermal wound dressing.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"15 1","pages":"e23626"},"PeriodicalIF":2.9,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142195210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Patch/Film for Drug Delivery—Current Status and Future Prospects 用于给药的口腔贴片/薄膜--现状与前景。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-09-04 DOI: 10.1002/bip.23625

Yujie Yan, Wenjie Yan, Sihua Wu, Hang Zhao, Qianming Chen, Jiongke Wang

引用次数: 0

Imparting of Nearly Superparamagnetic Properties to Cryogel Scaffolds With Mesoporous MNPs for Magneto-Sensitive Tissue Engineering Strategies 用介孔 MNPs 为冷凝胶支架赋予近超顺磁性能，以实现磁敏感组织工程策略。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-19 DOI: 10.1002/bip.23623

Didem Demir, Fatma Ulusal, Hasan Ulusal, Seda Ceylan, Sibel Dağlı, Nalan Özdemir, Mehmet Tarakçıoğlu

{"title":"Imparting of Nearly Superparamagnetic Properties to Cryogel Scaffolds With Mesoporous MNPs for Magneto-Sensitive Tissue Engineering Strategies","authors":"Didem Demir, Fatma Ulusal, Hasan Ulusal, Seda Ceylan, Sibel Dağlı, Nalan Özdemir, Mehmet Tarakçıoğlu","doi":"10.1002/bip.23623","DOIUrl":"10.1002/bip.23623","url":null,"abstract":"This work reports the assembly of mesoporous iron oxide nanoparticles (meso-MNPs) with cryogel scaffolds composed of chitosan and gelatin. Meso-MNPs with a particle size ranging from 2 and 50 nm, a surface area of 140.52 m2 g−1, and a pore volume of 0.27 cm3 g−1 were synthesized on a porous SiO2 template in the presence of PEG 6000 followed by leaching of SiO2. Different ratios of meso-MNPs were successfully incorporated into chitosan:gelatin cryogels up to an amount equivalent to the entire amount of polymer. The morphological structure and physicochemical properties of the cryogels were directly affected by the amount of MNPs. VSM curves showed that all composite cryogels could be magnetized by applying a magnetic field. In the context of the safety of magnetic cryogel scaffolds for use in biomedicine, it is important to note that all values are below the exposure limit for static magnetic fields, and according to cytotoxicity data, scaffolds containing meso-MNPs showed nontoxicity with cell viability ranging from 150% to 275%. In addition, microbial analysis with gram-negative and gram-positive bacteria showed that the scaffolds exhibited activity against these bacteria.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship Between Cryoprotectant Potential and Protein Hydration in Aqueous Zwitterionic Solutions 水性齐聚物溶液中的低温保护电位与蛋白质水合作用之间的关系

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-17 DOI: 10.1002/bip.23622

Takahiro Takekiyo, Shuto Yamada, Tetsuya Hirata, Takeru Ishizaki, Kosuke Kuroda, Yukihiro Yoshimura

{"title":"Relationship Between Cryoprotectant Potential and Protein Hydration in Aqueous Zwitterionic Solutions","authors":"Takahiro Takekiyo, Shuto Yamada, Tetsuya Hirata, Takeru Ishizaki, Kosuke Kuroda, Yukihiro Yoshimura","doi":"10.1002/bip.23622","DOIUrl":"10.1002/bip.23622","url":null,"abstract":"<div>\u0000 \u0000 The cryoprotectant potential of 3-(1-(2-(2-methoxyethoxy)ethyl)imidazol-3-io)butane-1-carboxylate (OE2imC3C) for proteins necessitates assessment to elucidate its relationship with protein hydration. To reveal this relationship, we assessed the protein stability (pre-freezing and post-thawing) and melting behavior in dilute aqueous protein–OE2imC3C solutions containing varying mole fractions (x) of OE2imC3C (x = 0, 7.7 × 10−3, and 1.7 × 10−2) using Fourier-transform infrared (FTIR) and near-UV circular dichroism (near-UV CD) spectroscopy and differential scanning calorimetry (DSC) techniques. Following freezing/thawing using a deep freezer, protein stability in aqueous OE2imC3C solutions (x = 1.7 × 10−2) preserved the folded state owing to the protein–OE2imC3C interaction, whereas stability at x = 7.7 × 10−3 was reduced. These results indicate that the protein cryoprotectant potential in aqueous OE2imC3C solutions at x = 1.7 × 10−2 is higher than that at x = 7.7 × 10−3, owing to the preferential binding of OE2imC3C with proteins.\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Alkali Treatment Conditions of Ramie Fabrics Using Box–Behnken Method 使用箱式贝肯法优化苎麻织物的碱处理条件

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-12 DOI: 10.1002/bip.23621

Bewuket Teshome Wagaye, Jiansheng Guo

{"title":"Optimization of Alkali Treatment Conditions of Ramie Fabrics Using Box–Behnken Method","authors":"Bewuket Teshome Wagaye, Jiansheng Guo","doi":"10.1002/bip.23621","DOIUrl":"10.1002/bip.23621","url":null,"abstract":"<div>\u0000 \u0000 Ramie is a widely used plant fiber for making textiles and reinforcement in biodegradable composites. Pretreating cellulosic fibers with alkali before producing composites is increasingly used to enhance adhesion with polymeric resin. In this work, response surface methodology (RSM) based on the Box–Behnken technique was utilized to investigate the impact of independent variables on ramie fabric characteristics and determine the optimal treatment condition. The impact of alkali concentration, treatment time, and temperature on the breaking load and elongation at break of woven ramie fabrics were evaluated using Design-Expert software, which established the design matrix and analyzed the experimental data employing numerical and graphical optimization methods. Moreover, the impact of alkali treatment conditions on the surface morphology, structural change of ramie fabrics, and thermal properties was investigated. Based on the analysis of variance (ANOVA) results, the suggested quadratic models can adequately predict the breaking load and elongation at break of the ramie woven fabrics within the range of conditions applied in this investigation. The RSM revealed that an alkali concentration of 6.12%, a treatment time of 30 min, and a temperature of 39.13°C resulted in an optimum treatment condition with a breaking load of 518.28 N and elongation at break of 23.36%.\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of High Toughness Poly(3-Hydroxypropionate)-Based Block Copolymers With Poly(D-2-Hydroxybutyrate) and Poly(D-Lactate) Segments Using Evolved Monomer Sequence-Regulating Polyester Synthase 利用进化单体序列调节聚酯合成酶生物合成具有聚（D-2-羟基丁酸酯）和聚（D-乳酸酯）段的高韧性聚（3-羟基丙酸酯）基嵌段共聚物

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-08-08 DOI: 10.1002/bip.23618

Tomoya Kawakami, Hiroya Tomita, Phan Thi Hien, Ken'ichiro Matsumoto

{"title":"Biosynthesis of High Toughness Poly(3-Hydroxypropionate)-Based Block Copolymers With Poly(D-2-Hydroxybutyrate) and Poly(D-Lactate) Segments Using Evolved Monomer Sequence-Regulating Polyester Synthase","authors":"Tomoya Kawakami, Hiroya Tomita, Phan Thi Hien, Ken'ichiro Matsumoto","doi":"10.1002/bip.23618","DOIUrl":"10.1002/bip.23618","url":null,"abstract":"This study synthesized poly(3-hydroxypropionate) [P(3HP)]-containing polyhydroxyalkanoate (PHA) block copolymers, P(3HP)-b-P[2-hydroxybutyrate (2HB)] and P(3HP)-b-P(D-lactate) (PDLA), using Escherichia coli. The cells expressing an evolved sequence-regulating PHA synthase, PhaCARNDFH, and propionyl-CoA transferase were cultured with the supplementation of the corresponding monomer precursors in the medium. The block structure of P(3HP)-b-PDLA was confirmed by proton nuclear magnetic resonance analysis and solvent fractionation. The molecular weights of the polymers were in the range of 0.8–2.8 × 105. The solvent-cast polymer films were subjected to isothermal treatment to promote phase separation and crystallization and were subsequently melt-quenched to produce an amorphous phase. The melt-quenched P(3HP)-b-P(2HB) film exhibited a high elongation at break (1153%), resulting in a toughness of 181 MJ/m3. The solvent-cast film of P(3HP)-b-65 mol% PDLA exhibited partial elastic deformation, in which the P(3HP) phase functioned as a soft segment. The melt-quenching of the polymer resulted in embrittlement presumably due to the high lactate fraction. Overall, the P(3HP)-based block copolymers exhibited several mechanical properties depending on the higher-order structure of the polymer and the properties of the P(2-hydroxyalkanoate) segments. This study findings show that P(3HP)-b-P(2HB) and P(3HP)-b-PDLA can function excellently if their microstructures are properly controlled.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.23618","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141938921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0