Biopolymers最新文献_第10页

Protocols for in vitro reconstitution of the cyanobacterial circadian clock 蓝藻昼夜节律钟体外重组方案。

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-07-08 DOI: 10.1002/bip.23559

Archana Chavan, Joel Heisler, Yong-Gang Chang, Susan S. Golden, Carrie L. Partch, Andy LiWang

{"title":"Protocols for in vitro reconstitution of the cyanobacterial circadian clock","authors":"Archana Chavan, Joel Heisler, Yong-Gang Chang, Susan S. Golden, Carrie L. Partch, Andy LiWang","doi":"10.1002/bip.23559","DOIUrl":"10.1002/bip.23559","url":null,"abstract":"Circadian clocks are intracellular systems that orchestrate metabolic processes in anticipation of sunrise and sunset by providing an internal representation of local time. Because the ~24-h metabolic rhythms they produce are important to health across diverse life forms there is growing interest in their mechanisms. However, mechanistic studies are challenging in vivo due to the complex, that is, poorly defined, milieu of live cells. Recently, we reconstituted the intact circadian clock of cyanobacteria in vitro. It oscillates autonomously and remains phase coherent for many days with a fluorescence-based readout that enables real-time observation of individual clock proteins and promoter DNA simultaneously under defined conditions without user intervention. We found that reproducibility of the reactions required strict adherence to the quality of each recombinant clock protein purified from Escherichia coli. Here, we provide protocols for preparing in vitro clock samples so that other labs can ask questions about how changing environments, like temperature, metabolites, and protein levels are reflected in the core oscillator and propagated to regulation of transcription, providing deeper mechanistic insights into clock biology.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9818050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conformational fluctuations and phases in fused in sarcoma (FUS) low-complexity domain 融合肉瘤（FUS）低复杂度结构域的构象波动和相位。

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-07-03 DOI: 10.1002/bip.23558

D. Thirumalai, Abhinaw Kumar, Debayan Chakraborty, John E. Straub, Mauro L. Mugnai

{"title":"Conformational fluctuations and phases in fused in sarcoma (FUS) low-complexity domain","authors":"D. Thirumalai, Abhinaw Kumar, Debayan Chakraborty, John E. Straub, Mauro L. Mugnai","doi":"10.1002/bip.23558","DOIUrl":"10.1002/bip.23558","url":null,"abstract":"The well-known phenomenon of phase separation in synthetic polymers and proteins has become a major topic in biophysics because it has been invoked as a mechanism of compartment formation in cells, without the need for membranes. Most of the coacervates (or condensates) are composed of Intrinsically Disordered Proteins (IDPs) or regions that are structureless, often in interaction with RNA and DNA. One of the more intriguing IDPs is the 526-residue RNA-binding protein, Fused in Sarcoma (FUS), whose monomer conformations and condensates exhibit unusual behavior that are sensitive to solution conditions. By focussing principally on the N-terminus low-complexity domain (FUS-LC comprising residues 1–214) and other truncations, we rationalize the findings of solid-state NMR experiments, which show that FUS-LC adopts a non-polymorphic fibril structure (core-1) involving residues 39–95, flanked by fuzzy coats on both the N- and C-terminal ends. An alternate structure (core-2), whose free energy is comparable to core-1, emerges only in the truncated construct (residues 110–214). Both core-1 and core-2 fibrils are stabilized by a Tyrosine ladder as well as hydrophilic interactions. The morphologies (gels, fibrils, and glass-like) adopted by FUS seem to vary greatly, depending on the experimental conditions. The effect of phosphorylation is site-specific. Simulations show that phosphorylation of residues within the fibril has a greater destabilization effect than residues that are outside the fibril region, which accords well with experiments. Many of the peculiarities associated with FUS may also be shared by other IDPs, such as TDP43 and hnRNPA2. We outline a number of problems for which there is no clear molecular explanation.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9778979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient production of fluorophore-labeled CC chemokines for biophysical studies using recombinant enterokinase and recombinant sortase 利用重组肠激酶和重组分拣酶高效生产荧光团标记的 CC 趋化因子，用于生物物理研究。

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-06-21 DOI: 10.1002/bip.23557

Wenyan Guan, Ning Zhang, Arjan Bains, Mourad Sadqi, Cynthia M. Dupureur, Patricia J. LiWang

{"title":"Efficient production of fluorophore-labeled CC chemokines for biophysical studies using recombinant enterokinase and recombinant sortase","authors":"Wenyan Guan, Ning Zhang, Arjan Bains, Mourad Sadqi, Cynthia M. Dupureur, Patricia J. LiWang","doi":"10.1002/bip.23557","DOIUrl":"10.1002/bip.23557","url":null,"abstract":"Chemokines are important immune system proteins, many of which mediate inflammation due to their function to activate and cause chemotaxis of leukocytes. An important anti-inflammatory strategy is therefore to bind and inhibit chemokines, which leads to the need for biophysical studies of chemokines as they bind various possible partners. Because a successful anti-chemokine drug should bind at low concentrations, techniques such as fluorescence anisotropy that can provide nanomolar signal detection are required. To allow fluorescence experiments to be carried out on chemokines, a method is described for the production of fluorescently labeled chemokines. First, a fusion-tagged chemokine is produced in Escherichia coli, then efficient cleavage of the N-terminal fusion partner is carried out with lab-produced enterokinase, followed by covalent modification with a fluorophore, mediated by the lab-produced sortase enzyme. This overall process reduces the need for expensive commercial enzymatic reagents. Finally, we utilize the product, vMIP-fluor, in binding studies with the chemokine binding protein vCCI, which has great potential as an anti-inflammatory therapeutic, showing a binding constant for vCCI:vMIP-fluor of 0.37 ± 0.006 nM. We also show how a single modified chemokine homolog (vMIP-fluor) can be used in competition assays with other chemokines and we report a Kd for vCCI:CCL17 of 14 μM. This work demonstrates an efficient method of production and fluorescent labeling of chemokines for study across a broad range of concentrations.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10733556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9667286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond traditional therapy: Mucoadhesive polymers as a new frontier in oral cancer management 超越传统疗法:黏合剂聚合物作为口腔癌治疗的新前沿

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-06-21 DOI: 10.1002/bip.23556

Subhayan Das, Koushik Bhattacharya, Jonny J. Blaker, Nikhil K. Singha, Mahitosh Mandal

引用次数: 0

Alginate/chitosan hydrogels as perspective transport systems for cefotaxime 海藻酸盐/壳聚糖水凝胶作为头孢噻肟的前景转运系统。

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-06-15 DOI: 10.1002/bip.23555

Svetlana V. Shilova, Grigory M. Mirgaleev, Ksenia A. Romanova, Yury G. Galyametdinov

{"title":"Alginate/chitosan hydrogels as perspective transport systems for cefotaxime","authors":"Svetlana V. Shilova, Grigory M. Mirgaleev, Ksenia A. Romanova, Yury G. Galyametdinov","doi":"10.1002/bip.23555","DOIUrl":"10.1002/bip.23555","url":null,"abstract":"This work reports synthesis of pH-responsive alginate/chitosan hydrogel spheres with the average diameter of 2.0 ± 0.05 mm, which contain cefotaxime that is an antibiotic of the cefalosporine group. The spheres provided the cefotaxime encapsulation efficiency of 95 ± 1%. An in vitro release of cefotaxime from the spheres in the media that simulate human biological fluids in peroral delivery conditions was found to be a pH-dependent process. The analysis of cefotaxime release kinetics by the Korsmeyer–Peppas model revealed a non-Fickian mechanism of its diffusion, which may be related to intermolecular interactions occurring between the antibiotic and chitosan. Conductometry, UV spectroscopy, and IR spectroscopy were used to study complexation of chitosan with cefotaxime in aqueous media with varied pH, characterize the composition of the complexes, and calculate their stability constants. The composition of the cefotaxime–chitosan complexes was found to correspond to the 1.0:4.0 and 1.0:2.0 molar ratios of the components at pH 2.0 and 5.6, respectively. Quantum chemical modeling was used to evaluate energy characteristics of chitosan–cefotaxime complexation considering the influence of a solvent.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"114 10","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9642927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing paramagnetic NMR data on target DNA search by proteins using a discrete-state kinetic model for translocation 利用离散态易位动力学模型分析蛋白质搜索目标DNA的顺磁核磁共振数据。

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-05-31 DOI: 10.1002/bip.23553

Binhan Yu, Junji Iwahara

{"title":"Analyzing paramagnetic NMR data on target DNA search by proteins using a discrete-state kinetic model for translocation","authors":"Binhan Yu, Junji Iwahara","doi":"10.1002/bip.23553","DOIUrl":"10.1002/bip.23553","url":null,"abstract":"Before reaching their targets, sequence-specific DNA-binding proteins nonspecifically bind to DNA through electrostatic interactions and stochastically change their locations on DNA. Investigations into the dynamics of DNA-scanning by proteins are nontrivial due to the simultaneous presence of multiple translocation mechanisms and many sites for the protein to nonspecifically bind to DNA. Nuclear magnetic resonance (NMR) spectroscopy can provide information about the target DNA search processes at an atomic level. Paramagnetic relaxation enhancement (PRE) is particularly useful to study how the proteins scan DNA in the search process. Previously, relatively simple two-state or three-state exchange models were used to explain PRE data reflecting the target search process. In this work, using more realistic discrete-state stochastic kinetics models embedded into an NMR master equation, we analyzed the PRE data for the HoxD9 homeodomain interacting with DNA. The kinetic models that incorporate sliding, dissociation, association, and intersegment transfer can reproduce the PRE profiles observed at some different ionic strengths. The analysis confirms the previous interpretation of the PRE data and shows that the protein's probability distribution among nonspecific sites is nonuniform during the target DNA search process.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9553340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights from modelling studies and molecular dynamics simulations of the DIS5-S6 extracellular linker of the skeletal muscle sodium channel NaV1.4 骨骼肌钠通道NaV1.4的DIS5-S6细胞外连接子的建模研究和分子动力学模拟的新见解

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-05-31 DOI: 10.1002/bip.23540

Anna Robinson, Elaine Tao, Teresa Neeman, Benjamin Kaehler, Ben Corry

{"title":"New insights from modelling studies and molecular dynamics simulations of the DIS5-S6 extracellular linker of the skeletal muscle sodium channel NaV1.4","authors":"Anna Robinson, Elaine Tao, Teresa Neeman, Benjamin Kaehler, Ben Corry","doi":"10.1002/bip.23540","DOIUrl":"https://doi.org/10.1002/bip.23540","url":null,"abstract":"In the CryoEM-structure of the hSkMNaV1.4 ion channel (PDB:6AGF), the 59-residue DIS5-S6 linker peptide was omitted due to absence of electron density. This peptide is intriguing – comprised of unique sequence and found only in mammalian skeletal muscle sodium ion channels. To probe potential physiological and evolutionary significance, we constructed an homology model of the complete hSkMNaV1.4 channel. Rather than a flexible random coil potentiating drift across the channel, the linker folds into a compact configuration through self-assembling secondary structural elements. Analogous sequences from 48 mammalian organisms show hypervariability with between 40% and 100% sequence similarity. To investigate structural implications, sequences from 14 representative organisms were additionally modelled. All showed highly conserved N-and C-terminal residues closely superimposed, suggesting a critical functional role. An optimally located asparagine residue within the conserved region was investigated for N-linked glycosylation and MD simulations carried out. Results suggest a complex glycan added at this site in the linker may form electrostatic interactions with the DIV voltage sensing domain and be mechanistically involved in channel gating. The relationship of unique sequence, compact configuration, potential glycosylation and MD simulations are discussed relative to SkMNaV1.4 structure and function.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"114 7","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.23540","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50149318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high molecular weight silk fibroin scaffold that resists degradation and promotes cell proliferation 抗降解和促进细胞增殖的高分子量丝素蛋白支架

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-05-26 DOI: 10.1002/bip.23554

Mengmeng Wang, Ying Wang, Peng Pan, Xueping Liu, Wenjing Zhang, Cheng Hu, Mingzhong Li

{"title":"A high molecular weight silk fibroin scaffold that resists degradation and promotes cell proliferation","authors":"Mengmeng Wang, Ying Wang, Peng Pan, Xueping Liu, Wenjing Zhang, Cheng Hu, Mingzhong Li","doi":"10.1002/bip.23554","DOIUrl":"10.1002/bip.23554","url":null,"abstract":"The regulation of the biodegradation rate of 3D-regenerated silk fibroin scaffolds and the avoidance of premature collapse are important concerns for their effective applications in tissue engineering. In this study, bromelain, which is specific to sericin, was used to remove sericin from silk, and high molecular weight silk fibroin was obtained after the fibroin fibers were dissolved. Afterwards, a 3D scaffold was prepared via freeze-drying. The Sodium dodecyl sulfate–polyacrylamide gel electrophoresis results showed that the average molecular weight of the regenerated silk fibroin prepared by using the bromelain-degumming method was approximately 142.2 kDa, which was significantly higher than that of the control groups prepared by using the urea- and Na2CO3-degumming methods. The results of enzyme degradation in vitro showed that the biodegradation rate and internal three-dimensional structure collapse of the bromelain-degumming fibroin scaffolds were significantly slower than those of the two control scaffolds. The proliferation activity of human umbilical vein vascular endothelial cells inoculated in bromelain-degumming fibroin scaffolds was significantly higher than that of the control scaffolds. This study provides a novel preparation method for 3D-regenerated silk fibroin scaffolds that can effectively resist biodegradation, continuously guide cell growth, have good biocompatibility, and have the potential to be used for the regeneration of various connective tissues.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"114 7","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9791709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The basal and major pilins in the Corynebacterium diphtheriae SpaA pilus adopt similar structures that competitively react with the pilin polymerase 白喉杆菌 SpaA 螺旋体中的基本螺旋体和主要螺旋体采用类似的结构，能与螺旋体聚合酶发生竞争性反应。

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-05-25 DOI: 10.1002/bip.23539

Christopher K. Sue, Nicole A. Cheung, Brendan J. Mahoney, Scott A. McConnell, Jack M. Scully, Janine Y. Fu, Chungyu Chang, Hung Ton-That, Joseph A. Loo, Robert T. Clubb

{"title":"The basal and major pilins in the Corynebacterium diphtheriae SpaA pilus adopt similar structures that competitively react with the pilin polymerase","authors":"Christopher K. Sue, Nicole A. Cheung, Brendan J. Mahoney, Scott A. McConnell, Jack M. Scully, Janine Y. Fu, Chungyu Chang, Hung Ton-That, Joseph A. Loo, Robert T. Clubb","doi":"10.1002/bip.23539","DOIUrl":"10.1002/bip.23539","url":null,"abstract":"Many species of pathogenic gram-positive bacteria display covalently crosslinked protein polymers (called pili or fimbriae) that mediate microbial adhesion to host tissues. These structures are assembled by pilus-specific sortase enzymes that join the pilin components together via lysine-isopeptide bonds. The archetypal SpaA pilus from Corynebacterium diphtheriae is built by the CdSrtA pilus-specific sortase, which crosslinks lysine residues within the SpaA and SpaB pilins to build the shaft and base of the pilus, respectively. Here, we show that CdSrtA crosslinks SpaB to SpaA via a K139(SpaB)-T494(SpaA) lysine-isopeptide bond. Despite sharing only limited sequence homology, an NMR structure of SpaB reveals striking similarities with the N-terminal domain of SpaA (NSpaA) that is also crosslinked by CdSrtA. In particular, both pilins contain similarly positioned reactive lysine residues and adjacent disordered AB loops that are predicted to be involved in the recently proposed “latch” mechanism of isopeptide bond formation. Competition experiments using an inactive SpaB variant and additional NMR studies suggest that SpaB terminates SpaA polymerization by outcompeting NSpaA for access to a shared thioester enzyme–substrate reaction intermediate.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9558659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies 基于胶原-聚氨酯-海藻酸盐半互穿聚合物网络的智能水凝胶，用于软硬组织愈合、药物输送装置和抗癌治疗

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-04-18 DOI: 10.1002/bip.23538

Rosalina Lara-Rico, Claudia M. López-Badillo, Jesús A. Claudio-Rizo, Denis. A. Cabrera-Munguía, Juan J. Becerra-Rodríguez, Roberto Espinosa-Neira, Brenda R. Cruz-Ortiz

{"title":"Smart hydrogels based on semi-interpenetrating polymeric networks of collagen-polyurethane-alginate for soft/hard tissue healing, drug delivery devices, and anticancer therapies","authors":"Rosalina Lara-Rico, Claudia M. López-Badillo, Jesús A. Claudio-Rizo, Denis. A. Cabrera-Munguía, Juan J. Becerra-Rodríguez, Roberto Espinosa-Neira, Brenda R. Cruz-Ortiz","doi":"10.1002/bip.23538","DOIUrl":"10.1002/bip.23538","url":null,"abstract":"In this work, hydrogels based on semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate were studied physicochemically and from different approaches for biomedical application. It was determined that the matrices in the hydrogel state are crosslinked by the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker. The increment in alginate content (0–40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation. The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate. The matrices show a behavior of multidose release of ketorolac, and a higher concentration of analgesic is released in the semi-IPN matrix. The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%). The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact. Finally, the bioactivity of mineralization was evaluated to demonstrate that these hydrogels can induce the formation of carbonated apatite on their surface. The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"114 6","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2