Biopolymers最新文献

{"title":"Highly Compatible Nanocomposite-Based Bacterial Cellulose Doped With Dopamine and Titanium Dioxide Nanoparticles: Study the Effect of Mode of Addition, Characterization, Antibacterial, and Wound Healing Efficiencies","authors":"Fatma El. Zahraa M. Abdelhaq,&nbsp;Mohamed S. Hasanin,&nbsp;Mohamed O. Abdel-Monem,&nbsp;Nouran M. Abd El-Razek,&nbsp;Sawsan Dacrory,&nbsp;Ghada E. Dawwam","doi":"10.1002/bip.70025","DOIUrl":"https://doi.org/10.1002/bip.70025","url":null,"abstract":"<div>\u0000 \u0000 <p>Microbial resistance is an expenditure for a country's economy as a whole as well as its health systems. Metal oxide nanoparticles play a role in overcoming microbial resistance to antibiotics. Bacterial cellulose (BC) is a biopolymer that is friendly to the environment and has a wide range of economic uses, particularly in biomedicine. This work deals with the formulation of BC-doped titanium dioxide nanoparticles (TiO₂NPs) and polydopamine (DOP), which are presented with antimicrobial activity. Additionally, the mode of addition of the doped materials was studied using physicochemical analysis, including Fourier transform infrared spectroscopy (FTIR) and x-ray diffraction (XRD). Moreover, the topographical study used scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). The antimicrobial activity was studied and showed the efficiency of the BC/DOP/TiO₂NP composite against Gram-positive (<i>Staphylococcus aureus</i>) and Gram-negative (<i>Pseudomonas aeruginosa, Escherichia coli</i>) strains. Additionally, the wound healing was examined on rats that had been purposely wounded. The results observed that the mode of addition contributed to the molecular structure of the formulated BC-doped samples according to the physicochemical and topographical analysis. Moreover, the BC/DOP/TiO₂NP composite enhanced wound healing for about 95% closure by Day 14 compared to 50% in the control group. Based on the results, we can suggest BC/DOP/TiO₂NP as an excellent candidate for wound dressings.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143909387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel Based on Cellulose and Mangosteen Rind Extract With Antibacterial Activity: Preparation and Characterization","authors":"Ha Thu Nguyen,&nbsp;Thanh Thao Nguyen,&nbsp;Ha Thu Do,&nbsp;Linh Vu Khanh Bui,&nbsp;Tai Anh Nguyen,&nbsp;Hoa Thanh Nguyen,&nbsp;Thi Thuy Tran","doi":"10.1002/bip.70024","DOIUrl":"https://doi.org/10.1002/bip.70024","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, an efficient method to prepare antibacterial hydrogel from natural resources was carried out. The extraction of mangosteen rind was made. Silver nanoparticle was introduced to the hydrogel through the reduction of AgNO₃ with the mangosteen rind extract. Hydrogels were prepared through graft-copolymerization of acrylic acid on cellulose in the presence of ammonium persulfate as initiator, followed by crosslinking with N,<i>N</i>′-methylenebisacrylamide. The characterization of components, structure, and properties of the rind extract and the hydrogels was carried out. The solvent mixture of water:ethanol 1:2 (v/v) was the most suitable solvent for the extraction of mangosteen rind. The silver nanoparticle was found to form a strong interaction with the composition of the hydrogel, which resulted in the improvement of the thermal property and the sterilization of both gram-positive and gram-negative bacteria. When the silver content was 9.7 ± 0.2 wt%, the hydrogel achieved the highest thermal property and antibacterial activity.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Produced Water and Light Irradiation on the Composition of Exopolysaccharide Produced by L. amnigena Evaluated by Raman Spectroscopy","authors":"Anna Paula Lima Teixeira da Silva,&nbsp;Luiz Guilherme Pinheiro Soares,&nbsp;Lars Duarte Gulberg,&nbsp;Pedro Jorge Louro Crugeira,&nbsp;Paulo Fernando de Almeida,&nbsp;Adjaci Uchoa Fernandes,&nbsp;Landulfo Silveira Jr.,&nbsp;Antonio Luiz Barbosa Pinheiro","doi":"10.1002/bip.70022","DOIUrl":"https://doi.org/10.1002/bip.70022","url":null,"abstract":"<p>This study aimed to compare the changes in the composition of the exopolysaccharide (EPS) produced by <i>Lelliottia amnigena</i> in culture medium containing distilled water (DW) and dialyzed produced water (DPW) irradiated by either Laser (λ660 nm, 8.0 J/cm²) or LED (λ630 nm, 12.0 J/cm²) during bacterial growth using Raman spectroscopy at 1064 nm. The cultures of <i>L. amnigena</i> were irradiated at 9- and 12-h, and the EPS obtained from different production protocols were analyzed dehydrated. Raman spectra showed peaks assigned to saccharides from EPS polymer, and principal component analysis revealed differences in the composition of the EPS produced depending on the water used in production and the light source used for irradiation. Remarkably, the presence of acyl groups (acetyl and pyruvyl) in the mannose residues at the group DW and mannose without evidence of acetyl in the irradiated groups; the irradiated groups also presented evidence of carboxylate (succinyl).</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and Characterization of Plant Extract-Based Cell-Friendly and High Mechanical Strength Nanofiber Wound Dressings by Electrospinning Technique","authors":"Caglar Sivri,&nbsp;Gulseren Sakarya","doi":"10.1002/bip.70021","DOIUrl":"https://doi.org/10.1002/bip.70021","url":null,"abstract":"<div>\u0000 \u0000 <p>This study focused on the development of wound dressings. Plant active ingredients such as clover, chickweed, chamomile, garlic, liverwort, bitter melon, pine resin, marigold (<i>Calendula officinalis</i>), and St. John's Wort (<i>Hypericum perforatum</i> L.) were reinforced with polyethylene oxide (PEO) and polyvinyl alcohol (PVA) polymers, and nanofiber membranes were produced by electrospinning. As a result of the analyses, FTIR confirmed the presence of polymer and active ingredient functional groups in the composite membranes; softening and shifting were observed in the peaks. In the FEGSEM analysis, a thin and regular nanofiber structure was obtained in the S12 membrane in the range of 150–500 nm. In the tensile test, the tensile strength of the S12 sample was measured as 25.89 MPa, and this strength was associated with the homogeneous distribution and thinning of the fibers. In the mesenchymal stem cell analysis, cell viability was determined as 98%, and cell death was determined as 2% for the S12 membrane at the end of 72 h. The results show that the S12 composite membrane can be used as a biomaterial with ideal properties in wound healing applications.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Plasma and Ultrasound as Inclusion Complex Formation Method on the Functional Properties of Starch-Based Active Edible Coating","authors":"Pamela C. Flores-Silva,&nbsp;Ernesto A. García-González,&nbsp;Adrian Soler,&nbsp;Sandra V. Avila-Reyes,&nbsp;Ernesto Hernández-Hernández,&nbsp;José F. Hernández-Gámez,&nbsp;Israel Sifuentes-Nieves","doi":"10.1002/bip.70020","DOIUrl":"https://doi.org/10.1002/bip.70020","url":null,"abstract":"<div>\u0000 \u0000 <p>One way to contribute to sustainable food systems is to develop ecological processes to prepare ingredients that deliver bioactive compounds. In this study, we used cold plasma (CP) and ultrasound (US) as alternative methods to produce inclusion complexes (ICs) of β-cyclodextrin (β-CD) with orange and clove essential oils. Further, the performance of starch-based active edible coatings containing ICs on perishable foods like mushrooms was assessed. CP and US treatments allowed the essential oil molecules to enter the hydrophobic cavity of the β-CD, leading to rhomboidal particles and clusters. The addition of ICs decreases the amount of moisture in starch-based active edible coatings, promoting high barrier property against water vapor (from 3.5 E-05 to 2.18 E-05 g/m s Pa) and low moisture content in their monolayer (Xm) (from 4.40 to 10.124 g water/100 g.d.s) since the hydrophobic components (terpenes and phenolics) of essential oil decreased the number of active sites to bind water molecules. These coatings reduce the weight loss of mushrooms (from 72% to 53%) under storage conditions. Further investigation through life cycle assessment and eco-efficiency analysis will be useful in determining whether cold plasma and ultrasound are green technologies for obtaining cost-effective ICs for coating production. The results could be of interest to those who are looking to develop ICs by CP and US to enhance edible coatings functionality for the food packaging field.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Controlled-Release Biofertilizer Using Composite of Poly (Urea–Formaldehyde), Date Seeds and Sulfate of Potash-Magnesia (2MgSO4·K2SO4) as All-Biodegradable With Antimicrobial Potentials","authors":"Hoda M. El-Gharabawy,&nbsp;Hamada H. Abdel-Razik,&nbsp;Mostafa M. Gaafar","doi":"10.1002/bip.70016","DOIUrl":"https://doi.org/10.1002/bip.70016","url":null,"abstract":"<div>\u0000 \u0000 <p>Based on the combination of poly (urea-formaldehyde) (UF), Sulfate of Potash-Magnesia (Sul-Po-Mag) fertilizer (2MgSO₄·K₂SO₄), and Date Kernel Seed (DS), a novel slow-release fertilizer in the form of granules called UF/DS/Sul-Po-Mag composite was introduced. The IR, DSC, TG, and X-ray spectra were used to characterize the synthesized composite. Characterizations revealed that the new fertilizer had good compatibility and strong hydrogen-bond interactions with improved mechanical and slow-release properties. An aqueous medium and soil incubation studies (up to 70 days) were used to examine the slow-release behavior. Over time, the accessible SO4⁻², K⁺, and Mg⁺² contents showed significantly lower SO4⁻², K⁺, and Mg⁺² losses than conventional fertilizer, even for low-polymerized materials. The fertilizer composite proved significant antimicrobial activity against all tested pathogens using the broth dilution technique. The minimum inhibition concentration (MIC) for tested bacteria and yeasts was 20 mg, while the maximum inhibition concentration (MAC) ranges from 40 to 60 mg. On the other hand, MIC for tested filamentous fungi was 100 mg, while MAC was 200–500 mg. These antimicrobial properties against harmful microbes and nutritional contents would enhance the growth of beneficial microorganisms and maintain good equilibrium in the microbial community.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the Mechanism of Action of a Short, Synthetic Designer AMP Against Gram-Negative Bacteria","authors":"Sucharita Shadangi,&nbsp;Aditi Singh,&nbsp;Soumendra Rana","doi":"10.1002/bip.70019","DOIUrl":"https://doi.org/10.1002/bip.70019","url":null,"abstract":"<div>\u0000 \u0000 <p>Antimicrobial peptides (AMPs), produced in various organisms, including plants, as a first line of defense, are potent, functionally versatile, fast-acting small peptides with a net charge and diverse structures. Most AMPs demonstrate potent antibacterial activity, and AMPs with multimodal actions can potentially delay the development of antimicrobial resistance (AMR), one of the top 10 global public health challenges categorized by the WHO. Notably, the FDA has already approved several AMPs (Mol. Wt. ≤ 2 kDa) as antibiotics; however, there are not enough new-age antibiotics in the current pipeline to combat the looming problem of AMR in the clinic. Nevertheless, despite their potential, natural AMPs have their fair share of shortcomings for straightforward therapeutic applications. Therefore, extensive research on developing designer synthetic AMPs with broad-spectrum antimicrobial activity is currently being undertaken to mitigate the AMR challenge. In this context, we recently demonstrated a short synthetic designer AMP (SR17: ≤ 16 aa, mol. Wt. ≤ 2 kDa) that exhibits broad-spectrum bacteriostatic and bactericidal action against both gram-negative (<i>Escherichia coli, Pseudomonas aeruginosa</i>, <i>Acinetobacter baumannii</i>) and gram-positive (<i>Staphylococcus aureus</i>) bacteria. Interestingly, in gram-negative bacteria, the outer membrane proteins (OMPs) play a key role in transporting nutrients like iron from their surroundings through siderophores, which play a crucial role in various biochemical processes essential for their survival and growth. In the current study, the ability of SR17 to target the iron-transporting OMPs acting as the siderophore uptake system is investigated through computational techniques. A series of docking and molecular dynamics (MD) simulation studies involving iron transporters of various gram-negative bacteria indicate that SR17 can occupy the binding pocket in the OMPs necessary for binding of the iron-chelated siderophores, which is likely to prevent the further uptake of siderophores, affecting the growth and survival of the bacteria. Additionally, SR17 may potentially reach the bacterial cytoplasm by utilizing the siderophore uptake system and disrupt essential cytoplasmic processes, leading to the death of the bacteria, as observed in experimental studies.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the Synergistic Action of Irradiated Chitosan and Biocontrol Agents for the Management of Powdery Mildew in Grapevines","authors":"Komal R. Shinde,&nbsp;Tanaji K. Narute,&nbsp;Rakesh B. Sonawane,&nbsp;Vikas K. Bhalerao,&nbsp;Sunil G. Dalvi","doi":"10.1002/bip.70013","DOIUrl":"https://doi.org/10.1002/bip.70013","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to evaluate the synergistic action of electron beam irradiated chitosan and <i>Ampelomyces quisqualis</i> for the management of powdery mildew, the most significant disease incited by the obligate fungus <i>Erysiphe necator</i> Schw. (Formerly known as <i>Uncinula necator</i> (Schw.) Burr.) that causes substantial losses in grapes. In vivo field trials conducted during 2020-21 and 2021-22, the evaluation of irradiated chitosan and bioagent and fungicide for the efficient in managing the grape powdery mildew disease. The fungicide sulfur 80% WDG was determined to be the most efficient. However, it was followed by a friendly combination of irradiated chitosan (150 ppm) with <i>A. quisqualis</i> (0.5%). Eco-friendly molecules, that is irradiated chitosan 150 ppm with <i>A. quisqualis</i> (0.5%), were found to be the best alternative for chemical molecules to achieve the disease control 63.60% and were identified alternative to chemical treatments to manage the powdery mildew disease of grapes. Irradiated chitosan and biocontrol agents showed synergistic action for the management of powdery mildew in grapevines.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143822313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionic Liquid and Binary Solvent Assisted Preparation of Silk Fibroin and Polyethylene Glycol Film: Structural and Mechanical Properties","authors":"Jia Tee Low,&nbsp;Noor Izyan Syazana Mohd Yusoff,&nbsp;Mat Uzir Wahit,&nbsp;Norhayani Othman","doi":"10.1002/bip.70015","DOIUrl":"https://doi.org/10.1002/bip.70015","url":null,"abstract":"<div>\u0000 \u0000 <p>Silk fibroin (SF), a biodegradable and biocompatible material with excellent mechanical properties, is widely utilized in food packaging and medical applications. However, regenerated SF is inherently brittle, necessitating the addition of polyethylene glycol (PEG), a nontoxic and biocompatible plasticizer, to enhance its flexibility. In this study, SF-PEG films were fabricated using two solvent systems: a single solvent (1-butyl-3-methylimidazolium chloride, BMIM Cl) and a binary solvent system (BMIM Cl and dimethyl sulfoxide, DMSO). SL-PEG films were prepared using the single solvent, while SM-PEG films were produced with the binary solvent system. The structural, mechanical, and morphological properties of the films were compared. Results showed that the SM-PEG films exhibited excellent mechanical performance, with a tensile strength of 6.9 ± 0.7 MPa, a Young's modulus of 367.0 ± 42.9 MPa, and an elongation at break of 42.6% ± 4.0%, significantly outperforming the SL-PEG films. The enhanced performance of SM-PEG films was attributed to the improved dispersion of PEG within the SF matrix, facilitated by the binary solvent system. In conclusion, the binary solvent system effectively improved the flexibility and ductility of SF-PEG films, making them better suited for applications requiring robust and adaptable biomaterials, such as in food packaging and medical applications.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Impact of Diverse Salt Solvent Systems on the Structure and Functionality of Eggshell Membrane Proteins","authors":"Xinhua Liang,&nbsp;Honglian Cong,&nbsp;Haijun He","doi":"10.1002/bip.70017","DOIUrl":"https://doi.org/10.1002/bip.70017","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigated the effects of different solvent systems on the microstructure, roughness, and secondary structure of soluble eggshell membrane proteins (SEPs). The solvent system of CaCl₂/C₂H₅OH/H₂O produced tiny holes on the surface of SEP, and LiBr resulted in the formation of long holes. The saline solution increased the diameter of the protein fiber, the particle size of the solution, and the surface roughness of regenerated SEP films, mainly due to the enhanced intermolecular aggregation and precipitation. Zeta potential measurements indicated salts decreased the negative values and reduced the stability of the SEP solution. The different solutions showed similar circular dichroism waveforms. The peak intensity decreased at the positive and negative peaks, indicating that the triple helix structure of collagen was denatured to different degrees. Besides, the addition of salts decreased the content of α-helices and the β-turns and increased the content of β-sheets and random coils, indicating an increase in the disordered structure of the protein. This study contributes to a deeper understanding of the structural and functional relationships of eggshell membrane proteins, providing a vital basis for developing novel, eco-friendly, and multifunctional protein materials.</p>\u0000 </div>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}