Biopolymers最新文献

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148066607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Electropsun Composite Nanofibers of Ulvan/Chitosan/PVA: A Synergistic Approach to Enhance the Wound Healing Process\".","authors":"","doi":"10.1002/bip.70103","DOIUrl":"https://doi.org/10.1002/bip.70103","url":null,"abstract":"","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":"e70103"},"PeriodicalIF":3.2,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147925904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of the Adsorption Time on the Growth of Bovine Serum Albumin-Chondroitin Sulfate Multilayer Films: A Surface Plasmon Resonance Spectroscopy Study.","authors":"Angelos Pavlopoulos, Nikolitsa Katsenou, Aristeidis Papagiannopoulos, Dimitrios L Anastassopoulos, Nikolaos Spiliopoulos","doi":"10.1002/bip.70104","DOIUrl":"10.1002/bip.70104","url":null,"abstract":"<p><p>Multilayer films of bovine serum albumin (BSA) and chondroitin sulfate (CS) were assembled on gold substrates at pH 4.2 using the layer-by-layer (LbL) method, and their structural evolution was investigated via surface plasmon resonance (SPR) spectroscopy. To elucidate the role of adsorption kinetics in multilayer buildup, BSA adsorption times of 15, 30, 60, and 120 min (for each BSA adsorption cycle) were examined, while the CS adsorption time was kept constant at 15 min. Analysis of SPR reflectivity curves revealed two distinct growth regimes for all cases, an initial exponential followed by a linear regime. Longer BSA adsorption time promoted deeper chain infiltration, extending the exponential region and shifting the exponential-to-linear transition to higher bilayer numbers. In parallel, evaluation of the average dielectric constant of the polymer layer enabled quantification of the film volume fraction, which initially increased linearly before reaching a saturation plateau. The evolution of the adsorbed mass was inferred from the combined analysis of film thickness and volume fraction, revealing two distinct growth cases depending on the adsorption time. These cases reflect different relationships between the transition from exponential to linear growth and the saturation of the volume fraction. These findings demonstrate that BSA adsorption time is a key kinetic parameter governing chain diffusion, film compaction, and overall multilayer architecture. The results provide insight into the physicochemical mechanisms that dictate biopolymer film growth and offer a route for tuning the structural and optical properties of protein-polysaccharide coatings.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":"e70104"},"PeriodicalIF":2.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147926006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ascidians as a Sustainable Source of Cellulose: Physicochemical Characterization, Degradability, and Relevance for Bioplastic Applications.","authors":"Lizbeth Zamora-Mendoza, Yuliana J Valdiviezo-Cuenca, Gabriela Agurto-Rodríguez, Paúl O Guillén, Floralba López, Lady V Guanoliquin, Cesar Zambrano, Camilo Zamora-Ledezma, Jenny Rodríguez, Gabriel Gómez-Guerra, Frank Alexis","doi":"10.1002/bip.70099","DOIUrl":"10.1002/bip.70099","url":null,"abstract":"<p><p>Cellulose derived from ascidians (tunicates) is distinguished from plant-based counterparts by its marine origin, with high crystallinity, and complex hierarchical architecture. However, quantitative structure-property relationships governing its performance in bioplastic applications remain underexplored. Here, cellulose isolated from three ascidian species Ascidia sp. (T1), Herdmania cf. pallida (T2), and Ascidia sydneiensis (T3) was systematically characterized. X-ray diffraction reveals crystallinity indices (CrI) of 48% (T1) and 60% (T2, T3), the latter approaching values reported for highly ordered systems such as bacterial cellulose. Thermogravimetric analysis demonstrates species-dependent thermal stability, with maximum degradation temperatures of 345°C (T1) versus 400°C-401°C (T2, T3). Notably, T2 and T3 exhibit thermal behavior comparable to microcrystalline and bacterial cellulose, despite CrI values lower than those systems, indicating that hydrogen-bonding density and microfibrillar order govern thermal resilience. Scanning electron microscopy reveals distinct microfibrillar architectures, ranging from highly branched networks to compact laminar structures, which govern water interaction and mechanical response. Water absorption varies markedly by species: T1 and T3 absorb 2200-2400 wt% within 10 min, consistent with their branched, open fibrillar morphologies, whereas T2 absorbs only 1200 wt%, reflecting a compact lamellar microstructure that restricts water diffusion. Hydrolytic degradation after 28 days in neutral water remains minimal across all samples, confirming exceptional resistance to hydrolytic scission under mild conditions. Bioplastics fabricated from these celluloses exhibit tensile strengths of 1-4 MPa, directly correlating with microstructural packing. Collectively, these results establish that ascidian cellulose is the combination of thermal stability up to 400°C, tunable water affinity (1200-2400% absorption), and hydrolytic resistance (1-9% loss over 28 days) arises from species-specific interactions between crystallinity, hydrogen bonding, and microfibrillar architecture. This positions ascidian-derived cellulose as a distinct marine macromolecular scaffold for sustainable bioplastics where controlled water interaction and structural durability are required. In general, it is established the relationship between the biological origin, hierarchical structure, and macroscopic properties of tunicate cellulose, highlighting its potential as a marine-derived macromolecular building block suitable for sustainable bioplastics applications.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":"e70099"},"PeriodicalIF":2.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Boron Nitride Nanosheets on the Properties of Gelatin-Chitosan Bioinks for Extrusion-Based 3D Bioprinting.","authors":"Aykut Erdogan, Yigitcan Sumbelli, Yapincak Goncu, Nuran Ay","doi":"10.1002/bip.70101","DOIUrl":"10.1002/bip.70101","url":null,"abstract":"<p><p>In recent years, there has been increasing interest in developing functional bioink systems that better replicate biological and biochemical microenvironments while maintaining high print fidelity and cell viability. This study reports the development and characterization of gelatin-chitosan-based bioinks reinforced with hexagonal boron nitride nanosheets (BNNSs) for extrusion-based 3D bioprinting. Structural, chemical, and morphological analyses demonstrated the successful incorporation of BNNSs without the formation of new chemical bonds, while minor shifts in amide bands indicated enhanced hydrogen bonding and physical interactions. Rheological studies revealed that gelatin concentration was the primary factor governing viscosity, whereas BNNSs provided a composition-dependent reinforcing effect. Notably, at a low shear rate (0.001 s^-1), viscosity increased from 2865 Pa·s for low concentration gelatin bioinks to 9322 Pa·s for BNNS-containing high concentration formulations, representing more than a threefold increase. In contrast all formulations exhibited low viscosities below 3 Pa·s at 100 s^-1, confirming favorable extrusion behavior. Viscoelastic analysis further showed lower tan δ values for high gelatin content formulations, indicating elastic-dominant behavior and improved shape retention at printing temperatures. BNNS incorporation slightly reduced the glass transition temperature by approximately 10°C while preserving blend compatibility, contributing to enhanced thermal responsiveness and more uniform temperature distribution during printing. Printability analysis demonstrated that BNNSs improved shape fidelity in high-viscosity formulations, yielding printability index (Pr) values close to unity (Pr ≈ 0.94) and stable filament formation. Swelling and degradation studies showed that BNNS-containing 7.5% gelatin scaffolds exhibited reduced swelling and retained approximately 70% structural integrity after 14 days, whereas low-polymer formulations underwent rapid degradation. Cell viability assessments confirmed improved fibroblast adhesion and proliferation on BNNS-containing scaffolds. The incorporation of BNNSs improves the rheological and thermal characteristics of the gelatin-chitosan bioinks and positively influences cell response. These findings suggest that BNNS-containing formulations provide a more stable and thermally responsive printing platform.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":"e70101"},"PeriodicalIF":2.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mild Acidic Solvent Inducing Tobacco Stem Cellulose Nanocrystals for Fabrication of Film Materials With Enhanced Flexibility and Toughness.","authors":"Ze Liu, Huilin Chen, Jie Yang, Xuemei Li, Shiyun Tang, Ruijin Deng, Xiaoxi Si, Chunbo Liu, Li Zhu, Tao Zhang, Zhe Ling","doi":"10.1002/bip.70102","DOIUrl":"10.1002/bip.70102","url":null,"abstract":"<p><p>With rapid development of material science, application of lignocellulose and agricultural wastes to produce green and functional packaging materials has received much interest. Herein, tobacco stem cellulose nanocrystals (TC) were firstly prepared via facile and mild formic acid/choline chloride (FA/ChCl) deep eutectic solvent (DES) treatment. Systematic comparison of the TC nanomaterials with conventional cotton cellulose nanocrystals (CNCs) were performed from nanoscale to the applicable functions of the prepared films. Tobacco stem showed notable delignification after the FA/ChCl treatment with well-maintaining of xylan matrix, which induced more sphere (with average width of ~32 nm) and shorter nanocrystals after homogenization. The treatment also caused a series of crystalline structural variations, including reduced crystallinity and crystallite size, favorable for promoting molecular flexibility of TC. Vacuum-assisted drying induced the fabrication of the film materials. Even though TC films had less visible iridescence than CNCs films, they exhibited satisfying mechanical strength promotion, with breakage of ~30 MPa, and the elongation of ~14%, mainly due to the reduced rigidity, crystallinity and remaining xylan matrix. Therefore, the present study provided a novel approach to controllable fabricating high-quality cellulose nanocrystals for film materials fabrication, as well as a facile strategy for high-value added utilization of tobacco stem biomass.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":"e70102"},"PeriodicalIF":2.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Cellulose Renaissance: Reimagining Bacterial Bioproducts for Health and Earth.","authors":"Ajay Patel, Manisha Parmar, Payal Patel, Ritu Patel, Sujoy Bandyopadhyay, Hyosung Choi, Haren Gosai","doi":"10.1002/bip.70100","DOIUrl":"10.1002/bip.70100","url":null,"abstract":"<p><p>Bacterial cellulose (BC) is a natural nanofiber with numerous desirable properties, including porosity, flexibility, high water capacity, biocompatibility, and high crystallinity. BC is appealing to researchers in materials science and engineering due to its potential applications in healthcare, biosensing, food, and bioremediation. However, high production costs and inconsistencies in strain production have hindered large-scale production and commercial applications. BC production optimization is required to boost industrial-scale productivity while reducing production time and expenses. This review covers BC biosynthesis and assembly, nutrient requirements, and structure and properties. Strategies for improved BC production, either through conventional or statistical optimization, have been discussed. Genetic approaches to modify BC-producing strains to increase BC yield have been explored. Various applications of BC in medical and environmental sectors have been compiled here for better understanding. The authors have investigated BC's significance in day-to-day life and how they can be useful to society.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":"e70100"},"PeriodicalIF":2.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148094824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Freeze–Thaw-Induced Physically Cross-Linked Poly(Vinyl Alcohol)/Alginate Hydrogels Incorporating Silver-Doped Zinc Oxide and Clove Oil","authors":"Büşra Mutlu","doi":"10.1002/bip.70098","DOIUrl":"10.1002/bip.70098","url":null,"abstract":"<p>This study reports the fabrication and comprehensive characterization of multicomponent silver-doped zinc oxide (Ag/ZnO)–poly(vinyl alcohol)/alginate(PVA/Alg) biocomposite hydrogels enriched with clove oil (CO), prepared via a scalable and solvent-free freeze–thaw (F–T) process. The F–T treatment generated physically cross-linked hydrogel networks with preserved structural integrity and favorable mechanical performance. Morphological analysis revealed a homogeneous and interconnected porous architecture at low CO contents, while contact-angle measurements confirmed hydrophilic surfaces with composition-dependent wettability. X-ray diffraction and Fourier transform infrared spectroscopy verified the successful incorporation of Ag/ZnO and CO into the PVA/Alg matrix without disrupting the overall polymer network. Barrier performance was strongly governed by CO content. Increasing CO loading reduced water vapor transmission through the formation of hydrophobic domains, while oxygen permeability reached a maximum at 1 wt.% CO, highlighting tunable mass transport behavior. The hydrogels exhibited rapid swelling, sustained hydration stability for up to 72 h, and a composition-dependent CO release behavior. Collectively, these findings elucidate clear structure–processing–property relationships in Ag/ZnO-incorporated PVA/Alg hydrogels containing CO and demonstrate their promise as multifunctional materials for further investigation in wound dressing applications.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13051256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant Fibrinogen-Gamma-Chain as a Crosslinker of Thiol-ene Hydrogels","authors":"Domenic Schlauch,&nbsp;Charlotte Selin Güler,&nbsp;Marina Harzi,&nbsp;Selin Kara,&nbsp;Antonina Lavrentieva,&nbsp;Iliyana Pepelanova","doi":"10.1002/bip.70097","DOIUrl":"10.1002/bip.70097","url":null,"abstract":"<p>Hydrogels based on thiol-ene step-growth chemistry have gained increased attention due to their superior properties over the currently standard materials based on chain growth polymerization. In the thiol-ene reaction, a crosslinker with at least two thiol groups is necessary for network formation. Many currently used crosslinkers exhibit cytotoxic potential, are non-biodegradable, or involve toxic chemicals and relatively complicated procedures in their synthesis, thus hindering their broader application. As an alternative, the use of a protein (fibrinogen gamma chain, FGG) recombinantly expressed in <i>Escherichia coli</i> was investigated. The FGG is part of the multimeric fibrinogen involved in hemostasis. This protein complex is stabilized by disulfide crosslinking. This presence of cysteines in the sequence makes FGG a promising candidate as a thiol donor in thiol-ene reactions. It was shown for the first time, that a cysteine-containing protein expressed in <i>E. coli</i> was capable of forming hydrogels with norbornene functionalized gelatin. An increase in FGG concentration led to higher gel stiffness and a decrease in the swelling ratio. Furthermore, the material exhibited cell adhesive properties and biocompatibility. Overall, a proof-of-principle could be achieved, opening up the use of recombinant proteins without further modifications as crosslinkers in thiol-ene based hydrogels, providing a cost-effective, safe, and scalable material source.</p>","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"117 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}