Influence of Produced Water and Light Irradiation on the Composition of Exopolysaccharide Produced by L. amnigena Evaluated by Raman Spectroscopy

IF 3.2 4区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biopolymers Pub Date : 2025-04-30 DOI:10.1002/bip.70022

Anna Paula Lima Teixeira da Silva, Luiz Guilherme Pinheiro Soares, Lars Duarte Gulberg, Pedro Jorge Louro Crugeira, Paulo Fernando de Almeida, Adjaci Uchoa Fernandes, Landulfo Silveira Jr., Antonio Luiz Barbosa Pinheiro

{"title":"Influence of Produced Water and Light Irradiation on the Composition of Exopolysaccharide Produced by L. amnigena Evaluated by Raman Spectroscopy","authors":"Anna Paula Lima Teixeira da Silva, Luiz Guilherme Pinheiro Soares, Lars Duarte Gulberg, Pedro Jorge Louro Crugeira, Paulo Fernando de Almeida, Adjaci Uchoa Fernandes, Landulfo Silveira Jr., Antonio Luiz Barbosa Pinheiro","doi":"10.1002/bip.70022","DOIUrl":null,"url":null,"abstract":"This study aimed to compare the changes in the composition of the exopolysaccharide (EPS) produced by Lelliottia amnigena in culture medium containing distilled water (DW) and dialyzed produced water (DPW) irradiated by either Laser (λ660 nm, 8.0 J/cm2) or LED (λ630 nm, 12.0 J/cm2) during bacterial growth using Raman spectroscopy at 1064 nm. The cultures of L. amnigena were irradiated at 9- and 12-h, and the EPS obtained from different production protocols were analyzed dehydrated. Raman spectra showed peaks assigned to saccharides from EPS polymer, and principal component analysis revealed differences in the composition of the EPS produced depending on the water used in production and the light source used for irradiation. Remarkably, the presence of acyl groups (acetyl and pyruvyl) in the mannose residues at the group DW and mannose without evidence of acetyl in the irradiated groups; the irradiated groups also presented evidence of carboxylate (succinyl).","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"116 3","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.70022","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biopolymers","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bip.70022","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This study aimed to compare the changes in the composition of the exopolysaccharide (EPS) produced by Lelliottia amnigena in culture medium containing distilled water (DW) and dialyzed produced water (DPW) irradiated by either Laser (λ660 nm, 8.0 J/cm²) or LED (λ630 nm, 12.0 J/cm²) during bacterial growth using Raman spectroscopy at 1064 nm. The cultures of L. amnigena were irradiated at 9- and 12-h, and the EPS obtained from different production protocols were analyzed dehydrated. Raman spectra showed peaks assigned to saccharides from EPS polymer, and principal component analysis revealed differences in the composition of the EPS produced depending on the water used in production and the light source used for irradiation. Remarkably, the presence of acyl groups (acetyl and pyruvyl) in the mannose residues at the group DW and mannose without evidence of acetyl in the irradiated groups; the irradiated groups also presented evidence of carboxylate (succinyl).

Abstract Image

查看原文本刊更多论文

利用拉曼光谱研究采出水和光照对羊水菌胞外多糖组成的影响

利用1064 nm的拉曼光谱，比较了激光（波长为λ660 nm， 8.0 J/cm2）和LED（波长为λ630 nm， 12.0 J/cm2）辐照下，羊草（Lelliottia amnigena）在含有蒸馏水（DW）和渗析采出水（DPW）的培养基中产生的胞外多糖（EPS）的组成变化。对羊水乳杆菌培养物进行9 h和12 h的辐照处理，并对不同生产工艺获得的EPS进行脱水分析。拉曼光谱显示了EPS聚合物中糖的峰，主成分分析显示EPS的组成因生产用水和照射光源的不同而不同。值得注意的是，DW组甘露糖残基中存在酰基（乙酰基和丙酮基），而辐照组甘露糖残基中没有乙酰基的证据；辐照组也出现羧酸（琥珀酰）的证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biopolymers 生物-生化与分子生物学

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Founded in 1963, Biopolymers publishes strictly peer-reviewed papers examining naturally occurring and synthetic biological macromolecules. By including experimental and theoretical studies on the fundamental behaviour as well as applications of biopolymers, the journal serves the interdisciplinary biochemical, biophysical, biomaterials and biomedical research communities.