Biopolymers最新文献_第6页

Silk fibroin/vitreous humor hydrogel scaffold modified by a carbodiimide crosslinker for wound healing 经碳化二亚胺交联剂改性的丝纤维素/玻璃体水凝胶支架用于伤口愈合。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-07-12 DOI: 10.1002/bip.23612

Sepideh Rafiei, Marjan Ghanbari-Abdolmaleki, Reza Zeinali, Saeed Heidari-Keshel, Azam Rahimi, Farima Royanian, Davood Zaeifi, Kiana Taheri, Kimia Pourtaghi, Maryam Khaleghi, Esmaeil Biazar

{"title":"Silk fibroin/vitreous humor hydrogel scaffold modified by a carbodiimide crosslinker for wound healing","authors":"Sepideh Rafiei, Marjan Ghanbari-Abdolmaleki, Reza Zeinali, Saeed Heidari-Keshel, Azam Rahimi, Farima Royanian, Davood Zaeifi, Kiana Taheri, Kimia Pourtaghi, Maryam Khaleghi, Esmaeil Biazar","doi":"10.1002/bip.23612","DOIUrl":"10.1002/bip.23612","url":null,"abstract":"Natural-derived biomaterials can be used as substrates for the growth, proliferation, and differentiation of cells. In this study, bovine vitreous humor as a biological material was cross-linked to silk fibroin with different concentration ratios to design a suitable substrate for corneal tissue regeneration. The cross-linked samples were evaluated with different analyses such as structural, physical (optical, swelling, and degradation), mechanical, and biological (viability, cell adhesion) assays. The results showed that all samples had excellent transparency, especially those with higher silk fibroin content. Increasing the ratio of vitreous humor to silk fibroin decreased mechanical strength and increased swelling and degradation, respectively. There was no significant difference in the toxicity of the samples, and with the increase in vitreous humor ratio, adhesion and cell proliferation increased. Generally, silk fibroin with vitreous humor can provide desirable characteristics as a transparent film for corneal wound healing.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of polydopamine-modified cellulose hydrogel for controlled release of α-mangostin 制备聚多巴胺改性纤维素水凝胶，用于控制α-芒果菌素的释放。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-07-11 DOI: 10.1002/bip.23613

Hoang Lich Phan, Ngoc Cam Trang Tran, Thi Hoang Yen Le, Quoc-Viet Le, Tran-Thai-Duong Le, Ut Dong Thach

{"title":"Fabrication of polydopamine-modified cellulose hydrogel for controlled release of α-mangostin","authors":"Hoang Lich Phan, Ngoc Cam Trang Tran, Thi Hoang Yen Le, Quoc-Viet Le, Tran-Thai-Duong Le, Ut Dong Thach","doi":"10.1002/bip.23613","DOIUrl":"10.1002/bip.23613","url":null,"abstract":"Hydrogels are notable for their outstanding absorbent qualities, satisfactory compatibility with biological systems, ability to degrade, and inherent safety, all of which contribute to their high demand in the field of biomedicine. This study focuses on the fabrication of hydrogels using environmentally friendly cellulosic material. Cellulose hydrogel beads were prepared by physical cross-linking in a NaOH/urea medium. Furthermore, nano polydopamine was integrated into the hydrogel matrix as functional polymers and α-mangostin was employed as an active pharmaceutical ingredient. The physicochemical properties were comprehensively analyzed using Fourier-transform infrared spectrometer, 13C cross-polarization/magic angle spinning nuclear magnetic resonance, thermogravimetric analysis, and scanning electron microscope. The drug delivery properties, including water content, swelling ratio, and drug release profiles, were evaluated. In vitro cytotoxicity against MC3T3-E1 cells was assessed using sulforhodamine B staining. All test hydrogels exhibited inhibitory activity against the growth of MC3T3-E1 cells. These results indicated the potential use of these hydrogels as a drug delivery carrier for α-mangostin in the treatment of ankylosing spondylitis.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of cellulose nanocrystals from Zhombwe (Neorautanenia brachypus (harms) CA Sm.) bagasse Zhombwe（Neorautanenia brachypus (harms) CA Sm.）甘蔗渣中纤维素纳米晶体的表征。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-07-10 DOI: 10.1002/bip.23611

Rumbidzai A. Makanda, Zedias Chikwambi, Chrispen Murungweni, Hamilton Kakwere, Arnold B. Mashingaidze

{"title":"Characterization of cellulose nanocrystals from Zhombwe (Neorautanenia brachypus (harms) CA Sm.) bagasse","authors":"Rumbidzai A. Makanda, Zedias Chikwambi, Chrispen Murungweni, Hamilton Kakwere, Arnold B. Mashingaidze","doi":"10.1002/bip.23611","DOIUrl":"10.1002/bip.23611","url":null,"abstract":"Increased awareness of environmental pollution has changed focus to the use of biodegradable materials because they lack persistence in the environment. This article focused on the production of cellulose nanocrystals from Zhombwe, Neorautanenia brachypus (Harms) CA Sm. bagasse using steam explosion, alkaline treatment, bleaching, purification, and acid hydrolysis. The chemical composition after the treatments was determined using TAPPI standards. Further characterization was done using x-ray Diffraction (XRD), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). The nanoscale dimensions and morphology of the extracted nanocrystals was determined through field emission scanning electron microscopy (FE-SEM). FTIR spectroscopy and DSC confirmed the removal of noncellulosic compounds. XRD revealed that N. brachypus bagasse contained cellulose type I, which partly endured morphological change to polymorph II after purification and hydrolysis. FE-SEM revealed elliptical to rod-shaped structures after acid hydrolysis, which had a mean length and width of 1103 nm and 597 nm respectively. TAPPI tests revealed that successive chemical treatments increased crystallinity by 29.7%, enriched cellulose content by 74.2%, reduced lignin content by 21.7%, and reduced hemicellulose to less than 1%. The semicrystalline nature of the material produced in our work is a promising candidate for swelling hydrogel applications in areas such as wound dressing, heavy metal removal, controlled drug delivery, agriculture, and sanitary products. Future studies may focus on surface modification of nanocrystals to improve their thermal stability and therefore expand their range for potential industrial applications.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the architecture of starch granules revealed by iodine vapor binding and lintnerization. Part 1: Microscopic examinations 通过碘蒸气结合和线粒体化揭示淀粉颗粒的结构。第 1 部分：显微镜检查。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-07-02 DOI: 10.1002/bip.23610

Eric Bertoft, George Annor, Varatharajan Vamadevan, Amy Hui-Mei Lin

{"title":"On the architecture of starch granules revealed by iodine vapor binding and lintnerization. Part 1: Microscopic examinations","authors":"Eric Bertoft, George Annor, Varatharajan Vamadevan, Amy Hui-Mei Lin","doi":"10.1002/bip.23610","DOIUrl":"10.1002/bip.23610","url":null,"abstract":"Structural nature of glucan chains in the amorphous part of granular starch was examined by iodine vapor treatment and lintnerization. Four iodine-stained amylose-containing normal starches and their waxy counterparts were examined under a microscope before, during, and after lintnerization. The presence of amylose retarded the lintnerization rate. The degree of retardation correlated with the structural type of the amylopectin component, suggesting that potato amylopectin (type 4 structure) interacts with amylose in the granules, whereas in barley granules (type 1 structure) the interaction is very weak. The inclusion complexes with iodine were not degraded by the acid treatment. Therefore, the iodine-glucan chain complex formation could be used to study the structural nature of the flexible, amorphous parts of the starch granules. Indeed, at the end of lintnerization, when 20%–30% of the granules remained, substantial amounts of blue-stained complexes were washed out from the granules especially from amylose-containing barley and maize starch, but also from both normal and waxy cassava and potato starch. The complexation with iodine did not affect the rate of lintnerization. This suggested that single helical structures were present during lintnerization also in the absence of iodine and this conformation was the reason for the acid resistance.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 6","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of nanocellulose/reduced graphene oxide matrix loaded with cuprous oxide nanoparticles for efficient catalytic reduction of 4-nitrophenol 制备负载氧化亚铜纳米颗粒的纳米纤维素/还原氧化石墨烯基质，用于高效催化还原 4-硝基苯酚。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-06-26 DOI: 10.1002/bip.23608

Faouzia Khili, Amel Dakhlaoui Omrani

{"title":"Preparation of nanocellulose/reduced graphene oxide matrix loaded with cuprous oxide nanoparticles for efficient catalytic reduction of 4-nitrophenol","authors":"Faouzia Khili, Amel Dakhlaoui Omrani","doi":"10.1002/bip.23608","DOIUrl":"10.1002/bip.23608","url":null,"abstract":"The paper reports on the preparation of cellulose nanocrystals/reduced graphene oxide matrix loaded with cuprous oxide nanoparticles (CNC/rGO-Cu2O) through a simple solvothermal method and its application for 4-nitrophenol reduction to 4-aminophenol using sodium borohydride. The CNC/rGO-Cu2O nanocomposite was formed chemically by first mixing CNC and graphene oxide (GO) followed by complexation of the negatively charged functional groups of CNC/GO with Cu2+ ions and subsequent heating at 100°C. This resulted in the simultaneous reduction of GO to rGO and the formation of Cu2O nanoparticles. The as-elaborated nanocomposite was firstly characterized using different techniques such as atomic force microscopy, scanning electron microscopy, transmission electron microscopy, UV–Vis spectrophotometry, Raman spectroscopy and x-ray photoelectron spectroscopy. Then, it was successfully applied for efficient catalytic reduction of 4-nitrophenol to 4-aminophenol using sodium borohydride: the reduction was completed in about 6 min. After eight times use, the catalyst still maintained good catalytic performance. Compared to CNC/rGO, rGO/Cu2O and free Cu2O nanoparticles, the CNC/rGO-Cu2O nanocomposite exhibits higher catalytic activity even at lower copper loading.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-response of protein-polysaccharide multilayers adsorbed on a flat gold surface: A surface plasmon resonance study 吸附在平坦金表面的蛋白质-多糖多层膜的 pH 响应：表面等离子体共振研究。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-06-20 DOI: 10.1002/bip.23609

Nikolitsa Katsenou, Nikolaos Spiliopoulos, Dimitrios L. Anastassopoulos, Aristeidis Papagiannopoulos, Chris Toprakcioglu

{"title":"pH-response of protein-polysaccharide multilayers adsorbed on a flat gold surface: A surface plasmon resonance study","authors":"Nikolitsa Katsenou, Nikolaos Spiliopoulos, Dimitrios L. Anastassopoulos, Aristeidis Papagiannopoulos, Chris Toprakcioglu","doi":"10.1002/bip.23609","DOIUrl":"10.1002/bip.23609","url":null,"abstract":"Polysaccharide-protein multilayers (PPMLs) consisting of bovine serum albumin (BSA) and chondroitin sulfate (CS) are assembled in acidic solution (pH 4.2) via layer-by-layer deposition method. The formation of PPMLs on gold surface and their responsiveness to pH change from 4.2 to 7 is investigated by Surface Plasmon Resonance Spectroscopy. The buildup of the multilayer at pH 4.2 exhibits non-linear growth while the formation of the first layers is strongly affected by the physicochemical properties of the gold surface. Neutral solution (pH 7) affects the interactions between the biopolymers and results in a partially disassemble (disintegration) of the multilayer film. On one hand, the single pair of layers, BSA-CS and the double pair of layers, (BSA-CS)2, assemblies are stable in neutral pH, a result that will be of interest for biomedical applications. On the other hand, multilayer films consisting of more than four layers that is (BSA-CS)2<n<5, disintegrated down to the 4-layered structure by changing pH to neutral, a fact that renders the (BSA-CS)n assembly useful in the field of drug and protein delivery. The residual mass after the disintegration of the assembly never falls below the mass of four layers. The disintegrated multilayer film can be reconstructed and disassembled repeatedly, simply by cycling the pH value.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of bacterial cellulose/PVP nanofiber composites by electrospinning 利用电纺丝技术制造细菌纤维素/PVP 纳米纤维复合材料。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-06-18 DOI: 10.1002/bip.23606

Nevra Pelin Cesur, Kosar Zad Ghaffari Vahdat, Nelisa Türkoğlu Laçin

{"title":"Fabrication of bacterial cellulose/PVP nanofiber composites by electrospinning","authors":"Nevra Pelin Cesur, Kosar Zad Ghaffari Vahdat, Nelisa Türkoğlu Laçin","doi":"10.1002/bip.23606","DOIUrl":"10.1002/bip.23606","url":null,"abstract":"This study aimed to address a significant challenge in the application of bacterial cellulose (BC) within tissue engineering and regenerative medicine by tackling its inherent insolubility in water and organic solvents. Our team introduced a groundbreaking approach by utilizing zinc sulfate (ZnSO4) as a solvent to render BC soluble, a novel contribution to the literature. Subsequently, the obtained soluble BC was combined with varying concentrations of polyvinylpyrrolidone (PVP). Notably, we pioneered the fabrication of BC/PVP composite scaffolds with customizable fiber surface morphology and regulated degradation rates through the electrospun technique. Several key parameters, such as PVP concentration (8%, 15%, 12%, and 20% w/v), applied voltage (22, 15, and 12 kV), and a fixed nozzle-collector distance of 10 cm with a flow rate of 0.9 mL/h, were systematically evaluated so as to find the optimum parameter created BC/PVP product with electrospun. For electrospun BC/PVP products, a voltage of 12 kV was found to be optimal. Intriguingly, our findings revealed enhanced cell adhesion and proliferation in BC/PVP electrospun products compared with using PVP membranes alone. Specifically, cell viability for PVP and PVP/BC electrospun products was determined as 50.73% and 79.95%, respectively. In terms of thermal properties, the BC/PVP electrospun product exhibited a mass loss of 82.6% at 380°C, while PVP alone experienced 90.2% mass loss at around 280°C. Furthermore, the protein adhesion capacities were measured at 62.3 ± 1.2 μg for PVP and 99.4 ± 2 μg for BC/PVP electrospun products, whereas product showed no biodegradation over 28 days and had notable water retention capacity. In conclusion, our research not only successfully attained nanofiber morphology but also showcased enhanced cell attachment and proliferation on the BC/PVP electrospun product.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of irradiated chitosan and microbial agent to reduce downy mildew on grapevine cv. Thompson seedless 将辐照壳聚糖和微生物制剂结合使用，减少葡萄树上的霜霉病。汤普森无籽葡萄

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-06-18 DOI: 10.1002/bip.23603

Mahadev Khatal, Tanaji Narute, Rakesh Sonawane, Vikas Bhalerao, Sunil Dalvi

{"title":"Combination of irradiated chitosan and microbial agent to reduce downy mildew on grapevine cv. Thompson seedless","authors":"Mahadev Khatal, Tanaji Narute, Rakesh Sonawane, Vikas Bhalerao, Sunil Dalvi","doi":"10.1002/bip.23603","DOIUrl":"10.1002/bip.23603","url":null,"abstract":"Globally sustainable disease management ensuring high quality in grapes is in demand as it holds significant importance as a versatile fruit for consumption, winemaking, and production of various products such as grape juice, raisin, and grape-seed oil. The present paper reports a combination of nano-biotechnology as a promising strategy for enhancing plant health and fruit productivity in grapes combining Irradiated chitosan nanoparticles and bio-control agents. The Irradiated Chitosan with Bacillus subtilis and Trichoderma viridae and pesticides were evaluated for disease management. Percent disease index, percent disease control, and percent yield enhancement in Cymoxanil 8% + Mamcozeb 64% WP @ 0.2% treatment were as 17. 24%, 67.97% and 33.91% in 150 ppm Irradiated chitosan+B. subtilis were 19.83, 63.16, 30.41 and in Trichoderma 150 ppm Irradiated chitosan were 24.58, 54.33, and 27.40, respectively as compared to untreated crop with disease severity 53.84% PDI. Thus, irradiated chitosan and Bacillus subtilis elucidated a synergistic combination for residue-free efficient phytosanitary measures, which harnessed the strength of chitosan and bio-control agents for sustainable grape productivity. These findings will also pave the way for a deeper understanding of the synergistic interaction between Irradiated nanochitosan and bio-control agents for an eco-friendly and economically viable disease management strategy. The minimum temperature and morning relative humidity (RH I) had positive significance, with correlation coefficients of 0.484 and 0.485, respectively. The evening relative humidity (RH II) had a positive highly significant positive correlation coefficient of 0.664. Chitosan merits as a multiple stress tolerance enhancing agent that will further help in mitigating climate change adaptations in grapevines reducing reliance on chemical agro-inputs.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and characterization of bis-phosphonated polycarbohydrates 双膦酸化多碳水化合物的制备和表征。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-06-17 DOI: 10.1002/bip.23607

Kamila Sadowska, Marta Prześniak-Welenc, Marcin Łapiński

引用次数: 0

Exploration of the sensitization effect of Chaihu Shugan powder on chemotherapy for triple-negative breast cancer and its active ingredients 柴胡舒筋粉对三阴性乳腺癌化疗的增敏作用及其有效成分探讨

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-06-12 DOI: 10.1002/bip.23605

Wei Wei, Xiaofei Li, Zhiyuan Li

{"title":"Exploration of the sensitization effect of Chaihu Shugan powder on chemotherapy for triple-negative breast cancer and its active ingredients","authors":"Wei Wei, Xiaofei Li, Zhiyuan Li","doi":"10.1002/bip.23605","DOIUrl":"10.1002/bip.23605","url":null,"abstract":"Chemotherapy plays a crucial role in the clinical treatment of triple-negative breast cancer (TNBC), but drug resistance limits its clinical application. The active ingredients of Chaihu Shugan Powder (CSP; Bupleurum Liver-Coursing Powder), quercetin and luteolin, both belong to flavonoid compounds and have significant anti-tumor potential, which can promote chemotherapy sensitivity. However, the correlation between the two and TNBC paclitaxel (PTX) chemotherapy sensitivity is unknown. We collected herbal components of CSP from the TCMSP database, and screened effective molecules and corresponding targets. STRING database was utilized to construct a protein–protein interaction network combining effective molecules and target genes. The top 50 nodes ranked by affinity were chosen for subsequent functional analysis, and the drug-active ingredient-gene interaction network was established using Cytoscape software. Molecular docking was used to determine the small molecules that target TNBC PTX resistance. The “clusterProfiler” package was utilized for GO and KEGG enrichment analyses on the top 50 genes to determine the pathways affected by CSP. Cell counting and colony formation assays evaluated cell viability, IC50 values, and proliferation capacity. Flow cytometry tested PTX intracellular accumulation. Western blot assayed the expression of TNF pathway-related proteins. Active ingredients of CSP, quercetin and luteolin, could inhibit TNBC cell proliferation and promote PTX chemotherapy sensitization. Quercetin and luteolin repressed the TNF signaling pathway and promoted PTX chemotherapy sensitization. Quercetin and luteolin could inhibit TNBC cell proliferation and promote PTX chemotherapy sensitization through the TNF signaling pathway. Therefore, the use of quercetin and luteolin plus PTX treatment provides a prospective strategy for TNBC treatment.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0