Biopolymers最新文献_第6页

Bacterial nanocellulose: A versatile biopolymer production using a cost-effective wooden disc based rotary reactor 细菌纳米纤维素：利用经济高效的木盘式旋转反应器生产多功能生物聚合物。

IF 3.2 4区生物学

Biopolymers Pub Date : 2024-03-25 DOI: 10.1002/bip.23577

Ashish Jagtap, Syed G. Dastager

{"title":"Bacterial nanocellulose: A versatile biopolymer production using a cost-effective wooden disc based rotary reactor","authors":"Ashish Jagtap, Syed G. Dastager","doi":"10.1002/bip.23577","DOIUrl":"10.1002/bip.23577","url":null,"abstract":"Bacterial nanocellulose (BNC) has various unique qualities, including high mechanical strength, crystallinity, and high water-holding capacity, which makes it appropriate for a wide range of industrial applications. But its lower yield coupled with its high production cost creates a barrier to its usage. In this study, we have demonstrated the better yield of BNC from an indigenous strain Komagataeibacter rhaeticus MCC-0157 using a rotary disc bioreactor (RDB) having a wooden disc. The RDB was optimized based on the type of disc material, distance between the disc, and rotation speed to get the highest yield of 13.0 g/L dry material using Hestrin–Schramm (H–S) medium. Further, the bioreactor was compared for the BNC production using reported medium, which is used for static condition; the RDB showed up to fivefold increase in comparison with the static condition reported. Komagataeibacter rhaeticus MCC-0157 was previously reported to be one of the highest BNC producing stains, with 8.37 g/L of dry yield in static condition in 15 days incubation. The designed RDB demonstrated 13.0 g/L dry yield of BNC in just 5 days. Other characteristics of BNC remain same as compared with static BNC production, although the difference in the crystallinity index was observed in RDB (84.44%) in comparison with static (89.74%). For the first time, wooden disc was used for rotary bioreactor approach, which demonstrated higher yield of BNC in lesser time and can be further used for sustainable production of BNC at the industrial level.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ewing Sarcoma Related protein 1 recognizes R-loops by binding DNA forks 尤文肉瘤相关蛋白 1 通过结合 DNA 叉来识别 R 环。

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-03-21 DOI: 10.1002/bip.23576

Michelle A. Lay, Valery F. Thompson, Ajibola D. Adelakun, Jacob C. Schwartz

{"title":"Ewing Sarcoma Related protein 1 recognizes R-loops by binding DNA forks","authors":"Michelle A. Lay, Valery F. Thompson, Ajibola D. Adelakun, Jacob C. Schwartz","doi":"10.1002/bip.23576","DOIUrl":"10.1002/bip.23576","url":null,"abstract":"EWSR1 (Ewing Sarcoma Related protein 1) is an RNA binding protein that is ubiquitously expressed across cell lines and involved in multiple parts of RNA processing, such as transcription, splicing, and mRNA transport. EWSR1 has also been implicated in cellular mechanisms to control formation of R-loops, a three-stranded nucleic acid structure consisting of a DNA:RNA hybrid and a displaced single-stranded DNA strand. Unscheduled R-loops result in genomic and transcription stress. Loss of function of EWSR1 functions commonly found in Ewing Sarcoma correlates with high abundance of R-loops. In this study, we investigated the mechanism for EWSR1 to recognize an R-loop structure specifically. Using electrophoretic mobility shift assays (EMSA), we detected the high affinity binding of EWSR1 to substrates representing components found in R-loops. EWSR1 specificity could be isolated to the DNA fork region, which transitions between double- and single-stranded DNA. Our data suggests that the Zinc-finger domain (ZnF) with flanking arginine and glycine rich (RGG) domains provide high affinity binding, while the RNA recognition motif (RRM) with its RGG domains offer improved specificity. This model offers a rational for EWSR1 specificity to encompass a wide range in contexts due to the DNA forks always found with R-loops.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.23576","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140179288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of hydrogel microneedles in the oral cavity 水凝胶微针在口腔中的应用。

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-03-20 DOI: 10.1002/bip.23573

Yiyao He, Dawei He, Lin Fan, Song Ren, Lin Wang, Jiang Sun

引用次数: 0

In vitro and in vivo investigation of Argiope bruennichi spider silk-based novel biomaterial for medical use Argiope bruennichi 蜘蛛丝新型医用生物材料的体外和体内研究。

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-03-16 DOI: 10.1002/bip.23572

Seçil Karahisar Turan, Aysun Kılıç Süloğlu, Semra İde, Tuncay Türkeş, Nurhayat Barlas

{"title":"In vitro and in vivo investigation of Argiope bruennichi spider silk-based novel biomaterial for medical use","authors":"Seçil Karahisar Turan, Aysun Kılıç Süloğlu, Semra İde, Tuncay Türkeş, Nurhayat Barlas","doi":"10.1002/bip.23572","DOIUrl":"10.1002/bip.23572","url":null,"abstract":"As a natural and biocompatible material with high strength and flexibility, spider silk is frequently used in biomedical studies. In this study, the availability of Argiope bruennichi spider silk as a surgical suture material was investigated. The effects of spider silk-based and commercial sutures, with and without Aloe vera coating, on wound healing were evaluated by a rat dorsal skin flap model, postoperatively (7th and 14th days). Biochemical, hematological, histological, immunohistochemical, small angle x-ray scattering (SAXS) analyses and mechanical tests were performed. A. bruennichi silk did not show any cytotoxic effect on the L929 cell line according to MTT and LDH assays, in vitro. The silk materials did not cause any allergic reaction, infection, or systemic effect in rats according to hematological and biochemical analyses. A. bruennichi spider silk group showed a similar healing response to commercial sutures. SAXS analysis showed that the 14th-day applications of A. bruennichi spider silk and A. vera coated commercial suture groups have comparable structural results with control group. In conclusion, A. bruennichi spider silk is biocompatible in line with the parameters examined and shows a healing response similar to the commercial sutures commonly used in the skin.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140139799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient heterogeneous synthesis of nucleophilic carboxymethyl hydrazides of polysaccharides 多糖亲核羧甲基酰肼的高效异构合成。

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-03-12 DOI: 10.1002/bip.23574

Hendryk Würfel, Annett Pfeifer, Thomas Heinze

引用次数: 0

Exploring helix structures of γ-peptides based on 2-(aminomethyl)cyclopentanecarboxylic acid 探索基于 2-(氨基甲基)环戊烷羧酸的 γ 肽的螺旋结构。

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-03-11 DOI: 10.1002/bip.23575

Hae Sook Park, Joo Yun Lee, Young Kee Kang

{"title":"Exploring helix structures of γ-peptides based on 2-(aminomethyl)cyclopentanecarboxylic acid","authors":"Hae Sook Park, Joo Yun Lee, Young Kee Kang","doi":"10.1002/bip.23575","DOIUrl":"10.1002/bip.23575","url":null,"abstract":"Conformational search and density functional theory calculations were performed to explore the preferences of helical structures for chiro-specific oligo-γ-peptides of 2-(aminomethyl)cyclopentanecarboxylic acid (γAmc5) with a cyclopentyl constraint on the Cα–Cβ bond in solution. The dimer and tetramer of γAmc5 (1) with homochiral (1S, 2S) configurations exhibited a strong preference for the 9-membered helix foldamer in solution, except for the tetramer in water. However, the oligomers of γAmc5 (1) longer than tetramer preferentially adopted a right-handed (P)-2.614-helix (H1-14) as the peptide sequence becomes longer and as solvent polarity increases. The high stabilities for H1-14 foldamers of γAmc5 (1) in solution were ascribed to the favored solvation free energies. The calculated mean backbone torsion angles for H1-14 helix foldamers of γAmc5 (1) were similar to those calculated for oligomers of other γ-residues with cyclopentane or cyclohexane rings. However, the substitution of cyclopentane constraints on the Cα−Cβ bond of the γAmc5 (1) residue resulted in different conformational preferences and/or handedness of helix foldamers. In particular, the pyrrolidine-substituted analogs of the H1-14 foldamers of γAmc5 (1) with adjacent amine diads substituted at a proximal distance are expected to be potential catalysts for the crossed aldol condensation in nonpolar and polar solvents.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.23575","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140093343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between the activation energy of PLA respectively PLA/starch composites and mechanical properties with regard to differ accelerated aging conditions 聚乳酸/淀粉复合材料的活化能分别与不同加速老化条件下的机械性能之间的相关性。

IF 2.9 4区生物学

Biopolymers Pub Date : 2024-02-22 DOI: 10.1002/bip.23571

Margarita Reit, Jan-Christoph Zarges, Hans-Peter Heim

{"title":"Correlation between the activation energy of PLA respectively PLA/starch composites and mechanical properties with regard to differ accelerated aging conditions","authors":"Margarita Reit, Jan-Christoph Zarges, Hans-Peter Heim","doi":"10.1002/bip.23571","DOIUrl":"10.1002/bip.23571","url":null,"abstract":"Within this research semi-crystalline polylactide and composites with 50 wt.% native potato starch were compounded and injection molded. The material was mechanically characterized by tensile, three-point bending, and Charpy impact tests. These tests were carried out in the freshly molded state and after 332 and 792 h of storage at accelerated temperature or humidity. The respective activation energy was calculated by applying the Flynn-Wall-Ozawa method. The focus of the study was to investigate the correlation between the activation energy and the related mechanical and thermal properties. The results showed that the addition of native potato starch as a filler prevents the decrease in activation energy over the course of the experiments. Thus, the PLA/starch composite is more resistant to the two aging conditions than the pure PLA. When considering the mechanical properties, the pure PLA showed a large deviation of results compared to the initial value in a range of +63.88% to −33.96% with regard to the respective aging conditions, whereas the PLA/starch composite properties nearly always remained at the initial values. Through the investigation of the mechanical and thermal properties, it was shown that the steady activation energies are consistent with the mechanical properties, as these have shown only a small deviation of the mechanical properties during the duration of experiments for the PLA/starch composite.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.23571","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139929866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The energy landscape of the ribosome 核糖体的能量景观

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-12-05 DOI: 10.1002/bip.23570

Sandra Byju, Asem Hassan, Paul C. Whitford

引用次数: 0

Structural, material and antibacterial properties of quercetin incorporated soy protein isolate films and its binding behavior through molecular docking 槲皮素结合大豆分离蛋白膜的结构、材料和抗菌性能及其分子对接的结合行为。

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-11-16 DOI: 10.1002/bip.23569

Priya Rani, Piyush Kumar Yadav, Ajay Kumar Singh, Suman Nayak, K. Dinesh Kumar, Rakesh Kumar

{"title":"Structural, material and antibacterial properties of quercetin incorporated soy protein isolate films and its binding behavior through molecular docking","authors":"Priya Rani, Piyush Kumar Yadav, Ajay Kumar Singh, Suman Nayak, K. Dinesh Kumar, Rakesh Kumar","doi":"10.1002/bip.23569","DOIUrl":"10.1002/bip.23569","url":null,"abstract":"This study aimed to investigate the three different methods for the fabrication of quercetin (1%–3% w/w of protein) incorporated soy protein isolate (SPI) films and their effect on material properties. The quercetin incorporated SPI films prepared by these methods were characterized by Fourier transform infrared (FTIR) spectroscopy, UV–Vis spectrophotometer, tensile properties, and water uptake and leaching properties. The cross-linking pattern was revealed by the FTIR spectrum that showed formation of an ester group because of interaction between the quercetin hydroxyl group and the carboxyl side chain of SPI amino acids. The tensile strength of SPI films were enhanced with the addition of quercetin as it increased to a maximum of 6.17 MPa while neat SPI film had tensile strength 4.13 MPa. The prepared films exhibit significant antibacterial activity against Listeria monocytogenes and Escherichia coli. The In-silico docking analysis demonstrates that covalent and non-covalent forces play crucial roles in binding interaction. It shows the formation of four hydrogen bonds, two salt bridges along with one pi-alkyl interaction. The simulation studies reflect the crucial amino acid residues involved in SPI-quercetin binding. The effect of quercetin binding with SPI on its stability and compactness is revealed by Root mean square deviation (RMSD) and radius of gyration studies.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"115 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136396032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovations in applications and prospects of non-isocyanate polyurethane bioplastics 非异氰酸酯聚氨酯生物塑料的应用创新与前景。

IF 2.9 4区生物学

Biopolymers Pub Date : 2023-10-17 DOI: 10.1002/bip.23568

Mangal Mangal, Supriya H., Suryasarathi Bose, Tamal Banerjee

{"title":"Innovations in applications and prospects of non-isocyanate polyurethane bioplastics","authors":"Mangal Mangal, Supriya H., Suryasarathi Bose, Tamal Banerjee","doi":"10.1002/bip.23568","DOIUrl":"10.1002/bip.23568","url":null,"abstract":"Currently, conventional plastics are necessary for a variety of aspects of modern daily life, including applications in the fields of healthcare, technology, and construction. However, they could also contain potentially hazardous compounds like isocyanates, whose degradation has a negative impact on both the environment and human health. Therefore, researchers are exploring alternatives to plastic which is sustainable and environmentally friendly without compromising its mechanical and physical features. This review study highlights the production of highly eco-friendly bioplastic as an efficient alternative to non-biodegradable conventional plastic. Bioplastics are produced from various renewable biomass sources such as plant debris, fatty acids, and oils. Poly-addition of di-isocyanates and polyols is a technique employed over decades to produce polyurethanes (PUs) bioplastics from renewable biomass feedstock. The toxicity of isocyanates is a major concern with the above-mentioned approach. Novel green synthetic approaches for polyurethanes without using isocyanates have been attracting greater interest in recent years to overcome the toxicity of isocyanate-containing raw materials. The polyaddition of cyclic carbonates (CCs) and polyfunctional amines appears to be the most promising method to obtain non-isocyanate polyurethanes (NIPUs). This method results in the creation of polymeric materials with distinctive and adaptable features with the elimination of harmful compounds. Consequently, non-isocyanate polyurethanes represent a new class of green polymeric materials. In this review study, we have discussed the possibility of creating novel NIPUs from renewable feedstocks in the context of the growing demand for efficient and ecologically friendly plastic products.","PeriodicalId":8866,"journal":{"name":"Biopolymers","volume":"114 12","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0