Biology of the Cell最新文献

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148081411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sibling Rivalry.","authors":"Christopher Thomas","doi":"10.1111/boc.70063","DOIUrl":"https://doi.org/10.1111/boc.70063","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 4","pages":"e70063"},"PeriodicalIF":2.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Interview With Louise Fougère. Winner of the SBCF PhD Award","authors":"Paul Trevorrow, Louise Fougere","doi":"10.1111/boc.70061","DOIUrl":"10.1111/boc.70061","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal Morphological Cell Studies","authors":"Albert Oppel","doi":"10.1111/boc.70057","DOIUrl":"10.1111/boc.70057","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healing Wounds","authors":"L. Anger,&nbsp;A. Harrouet,&nbsp;M. Hautefeuille,&nbsp;M. A. Fardin","doi":"10.1111/boc.70056","DOIUrl":"10.1111/boc.70056","url":null,"abstract":"<div>\u0000 \u0000 <p>Wound healing has long provided a framework for studying cell and tissue dynamics, yet early contributions are often overlooked. Revisiting research from the late 19th to mid-20th centuries reveals enduring questions on migration versus proliferation, individual versus collective motion, and chemical versus mechanical drivers. Concepts such as contractility, collective motility, and contact inhibition illustrate the continuity of debate across generations. By reconnecting these foundational studies with modern advances, we highlight persistent gaps and offer historical context to guide future research.</p>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implementation and Optimization of a Random Illumination Microscope: towards Robustness for Microscopy Core Facility","authors":"Nina Soler,&nbsp;Gilles Le Marchand,&nbsp;Stéphanie Dutertre,&nbsp;Xavier Pinson,&nbsp; Munish,&nbsp;Grégoire Michaux,&nbsp;Loïc Schmitt,&nbsp;Hélène Bouvrais,&nbsp;Claire Caron,&nbsp;Nicolas Jolivet,&nbsp;Giulia Bertolin,&nbsp;Simon Labouesse,&nbsp;Thomas Mangeat,&nbsp;Marc Tramier","doi":"10.1111/boc.70060","DOIUrl":"10.1111/boc.70060","url":null,"abstract":"<p>Super-resolution microscopy has become an indispensable tool for investigating molecular architectures in their native cellular environment. However, most super-resolution techniques face limitations that prevent rapid, deep imaging of live samples. Random Illumination Microscopy (RIM), based on natural laser speckle illumination, is a method of choice to overcome these challenges. RIM combines laser speckle illumination at the optical resolution with an algorithm that exploits the statistical invariance of speckle patterns.</p><p>In this approach, a stack of hundreds of random speckle images is acquired using a random diffusive element and then processed to reconstruct the super-resolved optical section. The invariant statistical properties of speckle patterns, which persist even as they diffuse through biological samples, enable deep-tissue imaging. Additionally, the wide-field configuration of both illumination and detection ensures high acquisition speeds and minimal sample photodamage.</p><p>Here, we present the implementation of our RIM prototype within a microscopy core facility. We describe the system setup, characterization, and optimization, identifying the key elements required for its reliable operation. As a proof of concept, we also provide biological examples demonstrating the prototype's performance in resolving subcellular structures.</p>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12968945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147376091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructure, Polarity, and Reproduction of the Golgi Apparatus","authors":"Bruno Goud","doi":"10.1111/boc.70053","DOIUrl":"10.1111/boc.70053","url":null,"abstract":"<p>In this note published in 1957 in the <i>Proceedings of the French Academy of Sciences</i>, the zoologist Pierre-Paul Grassé proposed for the first time that the cisternae of the Golgi apparatus form on the “cis” (proximal) face and are destroyed on the “trans” (distal) face to form chromophobe vesicles (presumably secretory granules), suggesting a model of maturation of the Golgi cisternae. This model is currently the most accepted by the cell biology community.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12967747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Interview With Marine Laporte, Winner of the SBCF Young Researcher Prize, 2025","authors":"Paul Trevorrow,&nbsp;Marine H. Laporte","doi":"10.1111/boc.70058","DOIUrl":"10.1111/boc.70058","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Robust Methodological Framework for Generating Whole-Brain and Cortical Organoids From Diverse Healthy- and Patient-Derived Induced Pluripotent Stem Cell Lines","authors":"Tara Fournier,&nbsp;Eugenia Pugliese,&nbsp;Benjamin Montagne,&nbsp;Chiara Cimmaruta,&nbsp;Miria Ricchetti","doi":"10.1111/boc.70051","DOIUrl":"10.1111/boc.70051","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Patient-derived neural organoids (NOs) have emerged as powerful tools for modeling human neurodevelopmental disorders, especially when animal models are unavailable or fail to recapitulate human-specific cortical development. However, significant variability in differentiation potential, even among healthy donor lines, and especially in fragile patient-derived induced pluripotent stem cells (iPSCs), poses major methodological challenges. Protocols that succeed in one line may fail in others, leading to poor organoid formation, reduced growth, impaired neuroepithelial patterning, or complete failure to generate neural tissues.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>By systematically comparing multiple published protocols, we identified key sources of variability and ultimately developed optimized protocols for generating both whole-brain (WB) and cortical organoids (CO) from human iPSCs (hiPSCs), including lines from progeroid Cockayne syndrome patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Through iterative refinement of critical parameters, including cell seeding density, Matrigel incorporation, and timing and type of pathway inhibition, we achieved consistent organoid growth and structural organization across all tested lines. The resulting pipeline is adaptable and can be further tailored for newly derived or particularly challenging hiPSC lines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Collectively, this methodological framework enables robust and reproducible generation of NOs from genetically diverse hiPSC sources, providing a reliable platform for studying human neurodevelopment and disease mechanisms in progeroid and other patient-specific contexts.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147316287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanobiology of the Nucleolus","authors":"Yuthika Shetty,&nbsp;Monika Elzbieta Dolega","doi":"10.1111/boc.70052","DOIUrl":"10.1111/boc.70052","url":null,"abstract":"<p>Ribosome biogenesis is an essential and energy-demanding process required to maintain cellular proteostasis. The nucleolus, which orchestrates this vital process, assembles as a nuclear condensate and exhibits remarkable plasticity in its structure, function, and protein composition. This dynamic membraneless organization of the nucleolus contributes to its involvement in diverse signaling pathways, influencing cell cycle regulation, proliferation, apoptosis, differentiation, and cellular stress response. Here, we focus on how mechanical cues influence nucleolar biology. Although classical mechanotransduction is mediated by membrane-anchored signaling proteins and the cytoskeleton that propagate forces within the cell, how mechanical cues influence the organization and function of dynamically assembled, membraneless condensates remains an open question. In this review, we explore how mechanical stimuli impact the nucleolus, the most prominent nuclear condensate. We examine how extrinsic forces alter nuclear structure, thereby affecting nucleolar organization, and function. Finally, we highlight emerging evidence that positions the nucleolus as a key mediator of nuclear mechano-adaptation, linking extracellular matrix (ECM) cues, migration, confinement, and external mechanical stress to nucleolar assembly and ribosome biogenesis.</p>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12949487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147316267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}