Biology of the Cell最新文献

{"title":"An Interview with Quentin Durieux-Trouilleton. Winner of the Sfμ Prix Favard Award","authors":"Paul Trevorrow, Quentin Durieux-Trouilleton","doi":"10.1111/boc.70055","DOIUrl":"10.1111/boc.70055","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147316244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional Activation of EGFL6 by E2F8 Promotes Proliferation, Migration, and Invasion in Endometrial Carcinoma Cell","authors":"Yeliu Fu,&nbsp;Zhilan Zhang","doi":"10.1111/boc.70050","DOIUrl":"10.1111/boc.70050","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Endometrial cancer (EC) posed a great threat to women's health, especially in postmenopausal women. E2F transcription factor 8 (E2F8), recognized as a central regulator of critical cellular processes, is overexpressed in endometrial carcinomas. However, the underlying mechanisms remains elusive and worthy of further investigation. Herein, we investigated whether E2F8 activates the expression of epidermal growth factor-like domain multiple 6 (EGFL6) through transcriptional regulatory mechanisms to regulate the occurrence and development of EC tumors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Western blot and quantitative reverse transcription polymerase chain reaction (qPCR) analyses revealed the expression profiles of E2F8 and EGFL6 in tumor tissues and EC cells. The Ishikawa and KLE cell lines were selected as in vitro models of EC and were subsequently transfected with sh-E2F8 or overexpression EGFL6 (oe-EGFL6) plasmids. CCK-8 assay and Transwell assay were performed to evaluate the cell viability, migration, and invasion of EC cells. A dual luciferase assay was conducted to assess the interaction between E2F8 and EGFL6. E2F8-knockdown Ishikawa cells were subcutaneously transplanted to investigate their effect on EC tumor growth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>E2F8 was greatly upregulated in EC cells. Silencing E2F8 inhibits the proliferation, migration, and invasion of EC cells, thereby suppressing tumor growth in vivo. Mechanistically, E2F8 transcriptionally activates EGFL6 by binding to its promoter. oe-EGFL6 rescues the impacts of E2F8 silencing on the proliferation, migration, and invasion of EC cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These results indicated that E2F8 promotes proliferation, migration, and invasion of EC cells by transcriptionally activating EGFL6.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"118 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146002976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling Mammary Gland Development and Reproductive Cycles With Organoids","authors":"Laurianne Temime,&nbsp;Lamia Djoual,&nbsp;Aurelie Chiche,&nbsp;Han Li","doi":"10.1111/boc.70048","DOIUrl":"10.1111/boc.70048","url":null,"abstract":"<div>\u0000 \u0000 <p>Mammary organoids bridge the gap between reductionist 2D systems and in vivo models by recapitulating bilayered epithelial architecture, branching, hormone responsiveness, and, in advanced platforms, functional readouts of lactation. This review synthesizes organoid models across the reproductive cycle, including branching morphogenesis, pregnancy-induced alveologenesis and milk secretion, and involution; it also surveys emerging directions, including embryonic/pluripotent and cross-species systems, as well as co-culture and organ-on-chip platforms that incorporate stromal, adipose, and immune elements. We outline priorities for building more complex, physiologically faithful ex vivo models that will enable mechanistic dissection of mammary development, yield comparative and translational insights, and create scalable platforms for perturbation and screening, advancing lactation research, breast cancer studies, and women's health in general.</p>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 12","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145833199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct Profiling of Extracellular Vesicle Surface Markers in Plasma: A Proof-of-Concept Study","authors":"Lee-Ann Clegg,&nbsp;Rebecca Best Jensen,&nbsp;Rikke Bæk,&nbsp;Birgitte Hviid Mumm,&nbsp;Maria Kjølhede Sørensen,&nbsp;Rolf Ankerlund Blauenfeldt,&nbsp;Kim Ryun Drasbek,&nbsp;Malene Møller Jørgensen","doi":"10.1111/boc.70047","DOIUrl":"10.1111/boc.70047","url":null,"abstract":"<p>During the last decade, non-invasive methods such as liquid biopsy have increasingly replaced invasive techniques for diagnosing and monitoring diseases. Liquid biopsies are easier to obtain, highly sensitive, cost-effective per sample, and can be used for real-time monitoring, such as tracking drug response. To uncover molecular and cellular characteristics today, blood, saliva, urine, or cerebrospinal fluid are typically analyzed. In this methodological proof-of-concept study, we propose that patient profiling can be facilitated by assessing surface markers on extracellular vesicles (EVs) directly in plasma without prior purification. EV composition reflects the physiological or pathological state of their parent cell, making EVs valuable tools for biomarker discovery and understanding disease mechanisms. Plasma and purified EVs from 30 ischemic stroke patients were analyzed for 17 surface markers using an in-house protein microarray method (EV Array). The findings indicate that measurements on EVs directly in plasma and on purified EVs show similar patterns in expression of surface markers, supporting the feasibility of omitting purification in EV profiling. This knowledge can provide faster monitoring of various diseases, prevent delays in patient profiling, and optimize patient care.</p>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 12","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12717633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145793168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome Tethering Requires Tetherin Homodimerisation","authors":"Yağmur Yıldızhan,&nbsp;Adam M. Bourke,&nbsp;Hannah K. Jackson,&nbsp;Paul T. Manna,&nbsp;Roberta Palmulli,&nbsp;James R. Edgar","doi":"10.1111/boc.70046","DOIUrl":"10.1111/boc.70046","url":null,"abstract":"<p>Exosomes are small extracellular vesicles that originate as intraluminal vesicles (ILVs) within multivesicular bodies (MVBs). Upon fusion of MVBs with the plasma membrane, ILVs are released into the extracellular environment as exosomes. Although exosomes can diffuse away from their cells of origin, the expression of the antiviral restriction factor tetherin promotes their retention at the cell surface, thereby limiting their release into the extracellular milieu. Tetherin plays an analogous role in retaining other extracellular particles, including many enveloped viruses and midbody remnants. We hypothesised that tetherin physically links exosomes to the cell surface through specific structural features of the protein. To test this, we combined biochemical assays with live-cell and ultrastructural imaging approaches to determine which elements of tetherin are required for exosome retention. Our analysis shows that the formation of tetherin homodimers is essential for exosome tethering. Mutations in regions or motifs of tetherin predicted to impact tetherin traffic to ILVs have only minor impacts on exosome tethering, suggesting redundancy in the mechanisms of tetherin traffic to ILVs, and subsequently, to exosomes. Collectively, these findings provide the first molecular insights into the mechanism that govern exosome tethering.</p>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 12","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12712889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of Intestinal and Colonic Organoids Derived From Human Pluripotent Stem Cells","authors":"Lola Bonneau,&nbsp;Lisa Brossard,&nbsp;Theo Noël,&nbsp;Victor Perreaux,&nbsp;Laura Bachir,&nbsp;Archie Khan,&nbsp;Simon Vales,&nbsp;Maxime M. Mahe","doi":"10.1111/boc.70044","DOIUrl":"https://doi.org/10.1111/boc.70044","url":null,"abstract":"<p>Over the past decade, significant advancements have been made in understanding the developmental mechanisms involved in human gastrointestinal formation, with organoids emerging as key experimental models. These three-dimensional in vitro cellular structures mimic the organization and functions of various gut regions, providing a powerful tool for research. By replicating critical stages of gut development, we can now direct the differentiation of cells into specific gastrointestinal tissues. In this protocol, we outline how to generate two types of organoids derived from human pluripotent stem cells (hPSCs): human intestinal organoids (HIOs) and human colonic organoids (HCOs). First, we induce definitive endoderm formation to produce these organoids and specify midgut/hindgut tissues. Three-dimensional spheroids form spontaneously, can be collected, embedded in an extracellular matrix, and cultured over time. During this phase, the organoid epithelium develops, supported by a mesenchymal layer that promotes maturation and differentiation. After a month of culture, HIOs and HCOs reach a developmental and maturation stage comparable to that of the human fetal intestine. These organoids can be used to study human gastrointestinal development, model diseases, and test therapeutic agents.</p>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 12","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/boc.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145739793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fasting Rescues Locomotion in Neuromodulation-Deficient C. elegans via Octopamine-Gαq Signaling","authors":"Jiayi He,&nbsp;Zi Wang,&nbsp;Guangshuo Ou,&nbsp;Wei Li","doi":"10.1111/boc.70043","DOIUrl":"10.1111/boc.70043","url":null,"abstract":"<div>\u0000 \u0000 <p>Nutrient deprivation induces adaptive behavioral and physiological changes that are critical for survival. Here, we demonstrate that fasting ameliorates locomotion defects in <i>Caenorhabditis elegans</i> mutants lacking UNC-31/CAPS, a protein essential for dense-core vesicle (DCV)-mediated neuromodulation. Through forward genetic screening, we identified a gain-of-function mutation in <i>egl-30</i>, which encodes the heterotrimeric G protein α subunit Gαq that suppresses the locomotion defects of <i>unc-31</i> mutants under fed conditions. Transcriptomic analyses revealed that fasting induces upregulation of <i>egl-30</i> and its downstream effectors in <i>unc-31</i> mutants. Remarkably, exogenous octopamine treatment, which activates EGL-30/Gαq signaling, mimicked the fasting response and restored locomotion in an EGL-30-dependent manner. Our findings uncover a mechanism of neuromodulatory plasticity, in which metabolic stress activates a compensatory octopamine-Gαq signaling cascade to bypass impaired DCV-mediated neuromodulation, and suggest potential therapeutic strategies for CAPS-related neuropsychiatric disorders.</p>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145595685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rewiring the Family Tree or Just Replacing the Powerhouse of the Cell","authors":"Karthikeyan D. Rajamani","doi":"10.1111/boc.70042","DOIUrl":"10.1111/boc.70042","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145538964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sestrin2 is Induced Upon Cellular Stress but Has No Effect on Myotube Size or Amino Acid Sensing in C2C12 Myotubes","authors":"Jiani Qian,&nbsp;Stephanie D. Gagnon,&nbsp;Vladimir Belhac,&nbsp;Carl J. Hulston,&nbsp;Neil R. W. Martin","doi":"10.1111/boc.70040","DOIUrl":"10.1111/boc.70040","url":null,"abstract":"<p>Sestrins are a stress-inducible family of proteins that function in cell survival and nutrient sensing through their regulation of mTORC1. Muscle wasting is associated with cellular stress, but to date, there is limited in vitro research investigating sestrins in skeletal muscle cells. Here we use C2C12 myotubes to understand how sestrin proteins (sestrin 1–3) are regulated by different forms of cellular stress linked to muscle wasting conditions. Furthermore, since sestrin2 is a well-characterised protein but is lowly expressed in muscle tissue in the absence of stress, we also aimed to determine if silencing this protein impacted parameters of muscle growth or nutrient sensing by mTORC1 under basal conditions. Incubating C2C12 myotubes with the endoplasmic reticulum (ER) stress-inducing agent tunicamycin, or a high concentration (1000 µM) of hydrogen peroxide (H₂O₂), increased sestrin2 protein levels with no change in sestrins 1 or 3. This increase was temporally associated with increased ER stress markers <i>Ddit3</i> mRNA and ATF4 protein levels, and could be blocked by approximately half when myotubes were co-incubated with H₂O₂ and the ER-stress inhibitor 4-Phenylbutyrate. siRNA silencing of sestrin2 blunted the phosphorylation of the mTORC1 effector S6K1, but did not acutely influence protein synthesis or myotube size. Similarly, silencing sestrin2 did not affect mTORC1 signalling in response to nutrient deprivation. These data indicate that sestrin2 is stress-inducible and may play a role in protecting skeletal muscle from ER stress, but is less important in regulating mTORC1 and nutrient sensing in unstressed/basal conditions.</p>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/boc.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145450810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid–Nucleic Acid Interactions: Shaping Genetic Information","authors":"María Moriel-Carretero","doi":"10.1111/boc.70039","DOIUrl":"https://doi.org/10.1111/boc.70039","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145398937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}