Revealing mitochondrial architecture and functions with single molecule localization microscopy

IF 2.4 4区 生物学 Q4 CELL BIOLOGY
Nicolas Jolivet, Giulia Bertolin
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引用次数: 0

Abstract

Understanding the spatiotemporal organization of components within living systems requires the highest resolution possible. Microscopy approaches that allow for a resolution below 250 nm include electron and super-resolution microscopy (SRM). The latter combines advanced imaging techniques and the optimization of image processing methods. Over the last two decades, various SRM-related approaches have been introduced, especially those relying on single molecule localization microscopy (SMLM). To develop and apply SMLM approaches, mitochondria are an ideal cellular compartment due to their size, which is below the standard diffraction limit. Furthermore, mitochondria are a dynamic yet narrow compartment, and a resolution below 250 nm is required to study their composition and multifaceted functions. To this end, several SMLM technologies have been used to reveal mitochondrial composition. However, there is still room for improvement in existing techniques to study protein–protein interactions and protein dynamics within this compartment. This review aims to offer an updated overview of the existing SMLM techniques and probes associated with mitochondria to enhance their resolution at the nanoscale. Last, it paves the way for future SMLM improvements to better resolve mitochondrial dynamics and functions.

Abstract Image

用单分子定位显微镜揭示线粒体结构和功能。
理解生命系统中组成部分的时空组织需要尽可能高的分辨率。分辨率低于250纳米的显微镜方法包括电子和超分辨率显微镜(SRM)。后者结合了先进的成像技术和优化的图像处理方法。在过去的二十年中,各种与srm相关的方法已经被引入,特别是那些依赖于单分子定位显微镜(SMLM)的方法。为了开发和应用SMLM方法,线粒体是理想的细胞隔室,因为它们的大小低于标准衍射极限。此外,线粒体是一个动态但狭窄的隔间,需要250 nm以下的分辨率来研究它们的组成和多方面的功能。为此,已经使用了几种SMLM技术来揭示线粒体组成。然而,在现有的技术中,研究蛋白质-蛋白质相互作用和蛋白质动力学在这个隔室中仍然有改进的空间。本综述旨在提供与线粒体相关的现有SMLM技术和探针的最新概述,以提高其在纳米尺度上的分辨率。最后,它为未来的SMLM改进铺平了道路,以更好地解决线粒体动力学和功能。
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来源期刊
Biology of the Cell
Biology of the Cell 生物-细胞生物学
CiteScore
5.30
自引率
0.00%
发文量
53
审稿时长
>12 weeks
期刊介绍: The journal publishes original research articles and reviews on all aspects of cellular, molecular and structural biology, developmental biology, cell physiology and evolution. It will publish articles or reviews contributing to the understanding of the elementary biochemical and biophysical principles of live matter organization from the molecular, cellular and tissues scales and organisms. This includes contributions directed towards understanding biochemical and biophysical mechanisms, structure-function relationships with respect to basic cell and tissue functions, development, development/evolution relationship, morphogenesis, stem cell biology, cell biology of disease, plant cell biology, as well as contributions directed toward understanding integrated processes at the organelles, cell and tissue levels. Contributions using approaches such as high resolution imaging, live imaging, quantitative cell biology and integrated biology; as well as those using innovative genetic and epigenetic technologies, ex-vivo tissue engineering, cellular, tissue and integrated functional analysis, and quantitative biology and modeling to demonstrate original biological principles are encouraged.
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