Biology of the Cell最新文献

{"title":"The crosstalk between exosomal miRNA and ferroptosis: A narrative review","authors":"Zahra Nashtahosseini,&nbsp;Masoumeh Nejatollahi,&nbsp;Ahmad Fazilat,&nbsp;Elahe Zarif Fakoor,&nbsp;Alireza Emamvirdizadeh,&nbsp;Kamran Bahadori,&nbsp;Niloofar Sadat Hadian,&nbsp;Mohammad Valilo","doi":"10.1111/boc.202400077","DOIUrl":"10.1111/boc.202400077","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 \u0000 <p>Ferroptosis is a type of cell death that multiple mechanisms and pathways contribute to the positive and negative regulation of it. For example, increased levels of reactive oxygen species (ROS) induce ferroptosis. ferroptosis unlike apoptosis, it is not dependent on caspases, but is dependent on iron. Exosomes are membrane-bound vesicles with a size of about 30 to 150 nm, contain various cellular components, including DNA, RNA, microRNAs (miRNAs), lipids, and proteins, which are genetically similar to their cells of origin. Exosomes are found in all bodily fluids, including blood, saliva, and urine. Cells often release exosomes after their fusion with the cell membrane. They play an important role in immune regulation and cell-cell communication. miRNAs, which are noncoding RNAs with a length of about 18 to 24 nucleotides, are involved in regulating gene expression after transcription. Emerging data suggests that exosomal miRNAs are implicated in various pathophysiological mechanisms of cells, including metastasis, drug resistance, and cell death. In addition, functional studies have indicated that exosomal miRNAs can play a key role in the modulation of cell death by regulating ferroptosis. Therefore, in this review, given the importance of exosomal miRNAs in ferroptosis, we decided to elucidate the relationship between exosomal miRNAs and ferroptosis in various diseases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolism-lipid droplet-nucleic acid crosstalk to regulate lipid storage and other cellular processes in oleaginous Rhodococcus bacteria","authors":"Héctor M. Alvarez,&nbsp;Mariana P. Lanfranconi,&nbsp;Martín A. Hernández","doi":"10.1111/boc.202400094","DOIUrl":"10.1111/boc.202400094","url":null,"abstract":"<p>Actinobacteria belonging to <i>Mycobacterium</i> and <i>Rhodococcus</i> genera are able to synthesize and intracellularly accumulate variable amounts of triacylglycerols (TAG) in the form of lipid droplets (LDs). The lipid storage capacity of LDs in cells is controlled by the balance between lipogenesis and lipolysis. The growth of LDs in bacterial cells may be directly promoted by TAG biosynthesis, whereas TAG degradation might result in the reduction of LD sizes and lipid storage capacity. Therefore, LD formation and turnover have to be precisely regulated to maintain a balanced lipid distribution, coupling gene regulation with the metabolic state of the cell. In eukaryotic cells, LDs have emerged as critical mediators of diverse cellular responses, including fatty acid trafficking and modulation of transcriptional programs. Recent studies performed in mycobacteria and rhodococci suggested the existence of similar crosstalk mechanisms between lipid metabolism, LDs, and gene expression regulation in cells. This review connects and organizes results of different studies in a comprehensive framework for providing evidence of “lipid metabolism-LDs-genomic DNA” crosstalk occurring in TAG-accumulating actinobacteria. We provide examples indicating that bacterial cells evolved sensing mechanisms that detect lipid metabolites changes as indicators of metabolic states, and adapt their transcriptional profiles through epigenetic-like mechanisms mediated by LD-associated proteins. Here, we describe the molecular interconnections of this coupling system and the main role of each component that integrates the information about the cellular metabolic state into the regulation of lipogenesis, LD formation and transcription in oleaginous bacteria.</p>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An interview with Meryem Baghdadi, winner of The French Society for Cell Biology (SBCF) Young Researcher Prize 2024","authors":"Meryem B. Baghdadi,&nbsp;Paul Trevorrow","doi":"10.1111/boc.202400159","DOIUrl":"10.1111/boc.202400159","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphoinositide signaling in the nucleus: Impacts on chromatin and transcription regulation","authors":"Nesrine Hifdi,&nbsp;Mathilde Vaucourt,&nbsp;Karim Hnia,&nbsp;Ganna Panasyuk,&nbsp;Marie Vandromme","doi":"10.1111/boc.202400096","DOIUrl":"10.1111/boc.202400096","url":null,"abstract":"<p>Phosphoinositides also called Polyphosphoinositides (PPIns) are small lipid messengers with established key roles in organelle trafficking and cell signaling in response to physiological and environmental inputs. Besides their well-described functions in the cytoplasm, accumulating evidences pointed to PPIns involvement in transcription and chromatin regulation. Through the description of previous and recent advances of PPIns implication in transcription, this review highlights key discoveries on how PPIns modulate nuclear factors activity and might impact chromatin to modify gene expression. Finally, we discuss how PPIns nuclear and cytosolic metabolisms work jointly in orchestrating key transduction cascades that end in the nucleus to modulate gene expression.</p>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing yeast cell analysis: A cryomethod for serial block-face scanning electron microscopy imaging in mitochondrial morphology studies","authors":"Corinne Blancard,&nbsp;Fanny Decoeur,&nbsp;Stéphane Duvezin-Caubet,&nbsp;Marie-France Giraud,&nbsp;Bénédicte Salin","doi":"10.1111/boc.202400038","DOIUrl":"10.1111/boc.202400038","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background Information</h3>\u0000 \u0000 <p>Conventional Transmission Electron Microscopy analysis of biological samples often provides limited insights due to its inherent two-dimensional (2D) nature. This limitation hampers a comprehensive understanding of the three-dimensional (3D) complexity of cellular structures, occasionally leading to misinterpretations. Serial block-face scanning electron microscopy emerges as a powerful method for acquiring high-resolution 3D images of cellular volumes. By iteratively removing ultrathin sample sections and capturing images of each newly exposed surface, Serial block-face scanning electron microscopy allows for the meticulous reconstruction of a comprehensive 3D volume.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In this study, we investigate the 3D architecture of altered mitochondrial morphologies in Saccharomyces cerevisiae using Serial block-face scanning electron microscopy imaging. We have developed a novel cryomethod based on plunge freezing and a dedicated freeze-substitution protocol.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This protocol enhances ultrastructural preservation enabling a more accurate understanding of mitochondrial defects observed in 2D electron microscopy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Significance</h3>\u0000 \u0000 <p>Our findings underscore the utility of Serial block-face scanning electron microscopy coupled with optimized sample preparation techniques in elucidating complex cellular structures in 3D.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear lipids in chromatin regulation: Biological roles, experimental approaches and existing challenges","authors":"Ahmed Sayed,&nbsp;Karthik Eswara,&nbsp;Kaian Teles,&nbsp;Ahlem Boudellioua,&nbsp;Wolfgang Fischle","doi":"10.1111/boc.202400103","DOIUrl":"10.1111/boc.202400103","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Lipids are crucial for various cellular functions. Besides the storage of energy equivalents, these include forming membrane bilayers and serving as signaling molecules. While significant progress has been made in the comprehension of the molecular and cellular biology of lipids, their functions in the cell nucleus remain poorly understood. The main role of the eukaryotic cell nucleus is to provide an environment for the storage and regulation of chromatin which is a complex of DNA, histones, and associated proteins. Recent studies suggest that nuclear lipids play a role in chromatin regulation and epigenetics. Here, we discuss various experimental methods in lipid-chromatin research, including biophysical, structural, and cell biology approaches, pointing out their strengths and weaknesses. We take the view that nuclear lipids have a far more widespread impact on chromatin than is currently acknowledged. This gap in comprehension is mostly due to existing experimental challenges in the study of lipid-chromatin biology. Several new, interdisciplinary approaches are discussed that could aid in elucidating the roles of nuclear lipids in chromatin regulation and gene expression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of aquaporin and Na+/K+-ATPase in gill and gut cells of the shrimp Palaemon argentinus regulated by ecdysone","authors":"Kamila Foguesatto,&nbsp;Celina C. Almeida,&nbsp;Robert T. Boyle,&nbsp;Luiz E. M. Nery,&nbsp;Marta M. Souza","doi":"10.1111/boc.202400085","DOIUrl":"10.1111/boc.202400085","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>The crustacean molting cycle is triggered by the elevation of ecdysteroid levels in the hemolymph during late pre-molt. It is known that these animals absorb water through the intestine and gills to promote bodily swelling and rupture of the old exoskeleton. The participation of two membrane proteins responsible for the most uptake of water during the late pre-molt has been shown in the gill and gut cells of the freshwater shrimp <i>Palaemon argentinus</i>: Na⁺/K⁺-ATPase (NKA), which generates an osmoionic gradient, and Aquaporins (AQPs), water channels, which provide higher water permeability. Studies investigating the action of ecdysteroids on these proteins are scarce. Therefore, we investigated 20-hydroxyecdisone (20E) in relation to the regulation of NKA and AQPs in the gill and gut cells of <i>P. argentinus</i>. We exposed primary cultures of both gut and gill cells to 20E. Gill cells treated with hormone in the presence of NKA-blockers, exhibited a reduction in volume, and cells treated with 20E showed a greater expression of NKA than untreated cells. Additionally, gills cells treated with 20E showed an increase in volume (∼60%), which the aquaporin inhibitor (HgCl₂) prevented. The participation of AQPs in the influx of water was corroborated by a greater expression of AQP in cells treated with 20E compared to untreated cells. Gut cells of animals in intermolt exposed to hormone (20E) maintained their initial cell volume. With the addition of HgCl2, these cells showed a reduction in volume similar to cells of animals in pre-molt. Immunocytochemistry showed a high expression of AQP in gut cells treated with 20E. These findings suggest that 20E regulates the expression of AQP and NKA in the late pre-molt, to provide water uptake for molting. This work offers new perspectives concerning the molting hormone, placing it as a crucial part of water uptake for ecdysis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"117 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRCI2NA inaugural symposium: A meeting on tumor and immune ecosystems","authors":"Vincent J. Guen,&nbsp;Gwennan André-Grégoire,&nbsp;Céline Beauvillain,&nbsp;Frank Boury,&nbsp;Morgane Chauvet,&nbsp;Aurore M. M. Dupuy,&nbsp;Jean-François Fonteneau,&nbsp;Katia Gagne,&nbsp;Julie Gavard,&nbsp;Patricia Gomez-Bougie,&nbsp;Eloïse Grasset,&nbsp;Jane Jardine,&nbsp;François Lamoureux,&nbsp;Mélanie Laurent--Blond,&nbsp;Éric Letouzé,&nbsp;Yanis Macé,&nbsp;Sandrine Maurice,&nbsp;Claire Pecqueur,&nbsp;Daniel Pouliquen,&nbsp;Latifa Rbah-Vidal,&nbsp;Catherine Pellat-Deceunynck,&nbsp;Philippe P. Juin","doi":"10.1111/boc.202400111","DOIUrl":"10.1111/boc.202400111","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>The CRCI²NA inaugural symposium, a meeting on tumor and immune ecosystems, took place in the vibrant and picturesque city of Nantes. The meeting gathered world-renowned experts in cancer biology and immunology. It showcased the most advanced science on mechanisms driving cellular heterogeneity, plasticity, and signaling in normal and cancer cellular ecosystems, which contribute to cancer development, progression, and therapeutic resistance. Recent developments in cancer immunotherapy and anti-tumor strategies were also discussed to collectively assess new therapeutic vulnerabilities to defeat cancer.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"116 12","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-terminal targeting sequences and coding sequences act in concert to determine the localization and trafficking pathway of apicoplast proteins in Toxoplasma gondii","authors":"Sofia Anjum,&nbsp;Aparna Prasad,&nbsp;Pragati Mastud,&nbsp;Geetanjali Mishra,&nbsp;Swati Patankar","doi":"10.1111/boc.202400027","DOIUrl":"10.1111/boc.202400027","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Backgound Information</h3>\u0000 \u0000 <p><i>Toxoplasma gondii</i> has a relict plastid, the apicoplast, to which nuclear-encoded proteins are targeted after synthesis in the cytosol. Proteins exclusively found in the apicoplast use a Golgi-independent route for trafficking, while dually targeted proteins found in both the apicoplast and the mitochondrion use a Golgi-dependent route. For apicoplast targeting, N-terminal signal sequences have been shown to direct the localization of different reporters. In this study, we use chimeric proteins to dissect out the roles of N-terminal sequences and coding sequences in apicoplast localization and the choice of the trafficking route.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We show that when the N-termini of a dually targeted protein, <i>Tg</i>TPx1/2, or of an apicoplast protein, <i>Tg</i>ACP, are fused with the reporter protein, enhanced green fluorescent protein (eGFP) or endogenous proteins, <i>Tg</i>SOD2, <i>Tg</i>SOD3, <i>Tg</i>ACP, or <i>Tg</i>TPx1/2, the chimeric proteins exhibit flexibility in apicoplast targeting depending on the coding sequences. Further, the chimeras that are localized to the apicoplast use different trafficking pathways depending on the combination of the N-terminal signals and the coding sequences.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion and Significance</h3>\u0000 \u0000 <p>This report shows, for the first time, that in addition to the N-terminal signal sequences, targeting and trafficking signals also reside within the coding sequences of apicoplast proteins.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"116 12","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meet our editorial board members: An interview with Tsuyoshi Hirashima, National University of Singapore","authors":"Paul Trevorrow,&nbsp;Tsuyoshi Hirashima","doi":"10.1111/boc.202400104","DOIUrl":"10.1111/boc.202400104","url":null,"abstract":"","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"116 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}