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Basic and applied histochemistry最新文献

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Immunophotometric quantification of extravascular immunoglobulin deposits in the synovial membrane of patients with osteoarthritis and rheumatoid arthritis. 骨关节炎和类风湿关节炎患者滑膜内血管外免疫球蛋白沉积的免疫光度定量。
Basic and applied histochemistry Pub Date : 1988-01-01
P Fritz, J Hönes, H Rautenstrauch, D Lutz, H V Tuczek, A Mischlinski, H J Saal, C Klein, W Laschner
{"title":"Immunophotometric quantification of extravascular immunoglobulin deposits in the synovial membrane of patients with osteoarthritis and rheumatoid arthritis.","authors":"P Fritz,&nbsp;J Hönes,&nbsp;H Rautenstrauch,&nbsp;D Lutz,&nbsp;H V Tuczek,&nbsp;A Mischlinski,&nbsp;H J Saal,&nbsp;C Klein,&nbsp;W Laschner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The amount of extravascular immunoglobulin deposits in the synovial membrane of patients with osteoarthritis and rheumatoid arthritis was studied in comparison with that of patients suffering from non-joint diseases. Immunoglobulin deposits were immunostained using the three-layer immunoperoxidase method. The staining results were quantified with the help of a microscope photometer connected with a scanning stage. Several experiments involving artificial test substrates, diseases with allegedly increased extravascular deposits or diseases not exhibiting extravascular deposits of immunoglobulins are used to validate the described microspectrophotometrical approach for measuring extravascular immunoglobulin deposits. The scanning photometry demonstrates significantly higher amount of extravascular immunoglobulin deposits in rheumatoid arthritis as compared with osteoarthritis and non-joint diseases.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14198139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histochemical localization of prostaglandin-synthetase in the salivary glands of Praomys (Mastomys) natalensis. 水貂唾液腺前列腺素合成酶的组织化学定位。
Basic and applied histochemistry Pub Date : 1988-01-01
P Sirigu, S A Gross, L J DiDio, M S Lantini
{"title":"Histochemical localization of prostaglandin-synthetase in the salivary glands of Praomys (Mastomys) natalensis.","authors":"P Sirigu,&nbsp;S A Gross,&nbsp;L J DiDio,&nbsp;M S Lantini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cellular distribution of prostaglandin-synthetase was studied in the salivary glands of Praomys (Mastomys) natalensis. Only the epithelial cells of the ducts showed discrete prostaglandin-synthetase activity, whereas the acini and the stroma gave completely negative results in all three salivary glands of both sexes. The presence of exogenous substrates was not required for the reaction, since identical results are observed following incubation with and without arachidonic acid. The localization of prostaglandin synthetase in the ductal epithelia of Praomys salivary glands is related to a possible regulatory role of the prostaglandins on the reabsorptive activity of the ductal cells.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14277874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative chemical anatomy of the brain: concepts and methods. 脑的比较化学解剖学:概念和方法。
Basic and applied histochemistry Pub Date : 1988-01-01
A Fasolo, G C Panzica, C Viglietti-Panzica, T Renda, L D'Este
{"title":"Comparative chemical anatomy of the brain: concepts and methods.","authors":"A Fasolo,&nbsp;G C Panzica,&nbsp;C Viglietti-Panzica,&nbsp;T Renda,&nbsp;L D'Este","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The study of neuropeptides represents an appropriate playground for comparative and evolutionary research. Comparative analysis can give insight into the conservative pattern of intercellular transmission molecules, possibly bound both to some evolutionary antiquity and to cellular constraints. In the same time it can teach us how modulation has occurred at molecular, cellular, multicellular levels in order to give the species-specific functional organization. Using some examples from vertebrate central neurons system (CNS) immunocytochemical analyses, the results so far obtained suggest the rise of a new comparative chemical neuroanatomy. The rationale of \"what\" and \"why\" we are comparing is, however, needed in order to understand constancy, heterogeneity or else trends toward complexity in the distribution of neuropeptides.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14514982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The peptidergic innervation of the guinea pig uterine artery in pregnancy. 妊娠期豚鼠子宫动脉的肽能神经支配。
Basic and applied histochemistry Pub Date : 1988-01-01
M C Mione, C Cavallotti, G Burnstock, F Amenta
{"title":"The peptidergic innervation of the guinea pig uterine artery in pregnancy.","authors":"M C Mione,&nbsp;C Cavallotti,&nbsp;G Burnstock,&nbsp;F Amenta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of pregnancy on the density and pattern of the peptidergic innervation of the guinea pig uterine artery was studied. Whole mount stretch preparations of the uterine artery from estrus and late pregnant guinea pigs were processed for the immunohistochemical demonstration of neuropeptide Y (NPY)-, vasoactive intestinal polypeptide (VIP)-, calcitonin gene-related peptide (CGRP)- and substance P (SP)- immunoreactive nerve fibres. In late pregnancy the density of NPY- and CGRP- containing nerve fibres was remarkably decreased, while that of VIP- and SP- immunoreactive nerves showed a moderate reduction. The meaning and the possible physiological relevance of the decreased density of peptide-immunoreactive nerves in the uterine artery in late pregnancy are discussed.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14514983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histological and immunohistochemical features in chronic delta hepatitis. 慢性丁型肝炎的组织学和免疫组织化学特征。
Basic and applied histochemistry Pub Date : 1988-01-01
C Mariscotti, P Viganò, R Costa, G Gubertini, A Cargnel
{"title":"Histological and immunohistochemical features in chronic delta hepatitis.","authors":"C Mariscotti,&nbsp;P Viganò,&nbsp;R Costa,&nbsp;G Gubertini,&nbsp;A Cargnel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Forty-six liver biopsies from HBsAg Delta/anti-Delta serum positive patients with chronic hepatitis were compared with liver specimens from HBsAg positive Delta/antiDelta negative patients. The results indicate more severe histologic damage and inflammation among subjects who are serum-positive to the Delta system. Specific virus antigen was found in liver cell nuclei of almost all the Delta IR patients (93.5%). The rate of diffusion was directly proportional to the severity of histological lesions, which is in linea with the direct cytopathic effect of the Delta virus as ascertained in various other studies.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14179142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Abstracts of the joint meeting of the Gruppo Italiano di Citometria and the Association de Cytometrie en Flux. Lerici, October 10-13, 1988. 意大利细胞计量学协会和通量细胞计量学协会联合会议摘要。莱里奇，1988年10月10日至13日。
Basic and applied histochemistry Pub Date : 1988-01-01
{"title":"Abstracts of the joint meeting of the Gruppo Italiano di Citometria and the Association de Cytometrie en Flux. Lerici, October 10-13, 1988.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14358885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection and differentiation of glycoconjugates in various cell types by lectin histochemistry. 凝集素组织化学对不同细胞类型糖结合物的检测和分化。
Basic and applied histochemistry Pub Date : 1988-01-01
S S Spicer, B A Schulte
{"title":"Detection and differentiation of glycoconjugates in various cell types by lectin histochemistry.","authors":"S S Spicer,&nbsp;B A Schulte","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Knowledge of chemical structure of glycoconjugates (GCs) at precise loci has increased through histochemical use of a battery of horseradish peroxidase-conjugated lectins, each possessing affinity for a specific terminal sugar or internal sugar linkage. Lectin histochemistry has shown tremendous variability among GCs in different histologic sites, reflecting known chemical diversity of these substances. GCs differ in structure among various cell types in an animal and differ at a given histologic site between species or between individuals in outbred but not inbred species. Lectin conjugates react with and detect GCs not otherwise demonstrable histochemically and, because of low concentration in tissue, not identified biochemically. Lectin-HRP conjugates have visualized unique GC with terminal GalNAc in primordial germ cells of rat embryos, with terminal Gal in epithelial basal cells of rodents and nodes of Ranvier in rats and with terminal GalNAc in a cell population in the thymus, Peyer's patches and intestinal lamina propria of some but not other mice.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14199078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Opening of blood-brain barrier in Triturus cristatus carnifex by hyperosmolar mannitol solutions. 高渗透压甘露醇溶液对黑麦血脑屏障的打开作用。
Basic and applied histochemistry Pub Date : 1988-01-01
V Franceschini, F Ciani, M Lazzari, P Del Grande, G Minelli
{"title":"Opening of blood-brain barrier in Triturus cristatus carnifex by hyperosmolar mannitol solutions.","authors":"V Franceschini,&nbsp;F Ciani,&nbsp;M Lazzari,&nbsp;P Del Grande,&nbsp;G Minelli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The permeability of the newt cerebral capillaries to lanthanum ion has been studied after perfusion with mannitol solutions of increasing molarity. In the control specimens lanthanum deposits were limited to the luminal side of the capillaries and tracer did not spread to the pericapillary spaces due to the tight junctions. Treatment with hypertonic solutions of mannitol (0.25M, 0.5M, 1M) caused opening of the blood brain barrier with a progressive increase in lanthanum between the endothelial cell edges, in the basal lamina and in the extracellular spaces of the nervous parenchyma in relation to the molarity of the mannitol solution. The spread of lanthanum is probably due to opening of the tight junctions between the endothelial cells, since pinocytotic vesicles labelled with tracer were not evident.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14277875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human gut neuroanatomy: methodology for a quantitative analysis of nerve elements and neurotransmitter diversity in the human "enteric nervous system". 人类肠道神经解剖学:人类“肠神经系统”中神经成分和神经递质多样性定量分析的方法学。
Basic and applied histochemistry Pub Date : 1988-01-01
G L Ferri
{"title":"Human gut neuroanatomy: methodology for a quantitative analysis of nerve elements and neurotransmitter diversity in the human \"enteric nervous system\".","authors":"G L Ferri","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>After a few decad of neglect, the \"enteric nervous system\" has recently regained the attention of investigators. Indeed, various studies, such as those which led to the isolation from the gut of a number of neuropeptides, subsequently demonstrated throughout the nervous system, have prompted major advancements of modern neuroscience. In spite of a wealth of animal investigations and a number of human studies, however, available information concerning the human \"enteric nervous system\" is comparatively sparse. In the opinion that such lack of information was largely due to unavailability of appropriate techniques, we have initiated and developed a new comprehensive methodology. This way, a quantitative analysis was made possible of both nerve structure and transmitter status, point-to-point along the gut, as well as within the various, functionally heterogeneous components of the gastrointestinal wall itself. After a general introduction, the present review is intended to summarize such methodology, with the addition of a few illustrative examples of application and a practically-oriented guideline to its use, in the form of technical appendix.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14034948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An immunocytochemical method for studying embryo cytokinetics. 研究胚胎细胞动力学的免疫细胞化学方法。
Basic and applied histochemistry Pub Date : 1988-01-01
A Casasco, A Calligaro, E Casasco, C Marchetti, P Poggi, M Casasco
{"title":"An immunocytochemical method for studying embryo cytokinetics.","authors":"A Casasco,&nbsp;A Calligaro,&nbsp;E Casasco,&nbsp;C Marchetti,&nbsp;P Poggi,&nbsp;M Casasco","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The immunocytochemical detection by a monoclonal antibody of bromodeoxyuridine incorporated into S-phase cells allows the identification of proliferating cells. In this study we demonstrate that the labelling of embryo tissues is achieved by a single administration of BrdUrd to the mother after a 1 hour-labelling period. This simple and rapid technique facilitates the detection of proliferating cells within the embryo for the study of developing tissues and embryo cytokinetics.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14541114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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