研究胚胎细胞动力学的免疫细胞化学方法。

Basic and applied histochemistry Pub Date : 1988-01-01
A Casasco, A Calligaro, E Casasco, C Marchetti, P Poggi, M Casasco
{"title":"研究胚胎细胞动力学的免疫细胞化学方法。","authors":"A Casasco,&nbsp;A Calligaro,&nbsp;E Casasco,&nbsp;C Marchetti,&nbsp;P Poggi,&nbsp;M Casasco","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The immunocytochemical detection by a monoclonal antibody of bromodeoxyuridine incorporated into S-phase cells allows the identification of proliferating cells. In this study we demonstrate that the labelling of embryo tissues is achieved by a single administration of BrdUrd to the mother after a 1 hour-labelling period. This simple and rapid technique facilitates the detection of proliferating cells within the embryo for the study of developing tissues and embryo cytokinetics.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"32 2","pages":"293-6"},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An immunocytochemical method for studying embryo cytokinetics.\",\"authors\":\"A Casasco,&nbsp;A Calligaro,&nbsp;E Casasco,&nbsp;C Marchetti,&nbsp;P Poggi,&nbsp;M Casasco\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The immunocytochemical detection by a monoclonal antibody of bromodeoxyuridine incorporated into S-phase cells allows the identification of proliferating cells. In this study we demonstrate that the labelling of embryo tissues is achieved by a single administration of BrdUrd to the mother after a 1 hour-labelling period. This simple and rapid technique facilitates the detection of proliferating cells within the embryo for the study of developing tissues and embryo cytokinetics.</p>\",\"PeriodicalId\":8726,\"journal\":{\"name\":\"Basic and applied histochemistry\",\"volume\":\"32 2\",\"pages\":\"293-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Basic and applied histochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Basic and applied histochemistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

通过将溴脱氧尿苷单克隆抗体纳入s期细胞的免疫细胞化学检测,可以识别增殖细胞。在这项研究中,我们证明胚胎组织的标记是通过在1小时标记期后单次给母BrdUrd来实现的。这种简单快速的技术有利于胚胎内增殖细胞的检测,用于发育组织和胚胎细胞动力学的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An immunocytochemical method for studying embryo cytokinetics.

The immunocytochemical detection by a monoclonal antibody of bromodeoxyuridine incorporated into S-phase cells allows the identification of proliferating cells. In this study we demonstrate that the labelling of embryo tissues is achieved by a single administration of BrdUrd to the mother after a 1 hour-labelling period. This simple and rapid technique facilitates the detection of proliferating cells within the embryo for the study of developing tissues and embryo cytokinetics.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信