Basic and applied histochemistry最新文献_第9页

Localization of different alcian blue-proteoglycan particles in the intervertebral disc. 不同阿利西亚蓝蛋白多糖颗粒在椎间盘的定位。

Basic and applied histochemistry Pub Date : 1988-01-01

F Ortolani, M Raspanti, M Franchi, M Marchini

{"title":"Localization of different alcian blue-proteoglycan particles in the intervertebral disc.","authors":"F Ortolani, M Raspanti, M Franchi, M Marchini","doi":"","DOIUrl":"","url":null,"abstract":"A histochemical investigation was carried out on proteoglycans of bovine intervertebral disc. Samples obtained from the \"annulus fibrosus\" (A.F.) and \"nucleus pulposus\" (N.P.) were treated with Alcian blue (AB) diluted in solutions of MgCl2 at critical electrolyte concentrations (CEC); some samples were incubated in testicular hyaluronidase before AB treatment. At least four types of elongated AB-proteoglycan particles were recognized: a) in A.F. lamellae and N.P., 1 nm rod-like particles were arranged orthogonally to the collagen fibrils and spaced at a distance equivalent to the fibril D-period (Figs. 1-5); b) within the A.F. lamellae, other 16-20 nm particles formed a close network among the collagen fibrils (Figs. 1,2,3,5); c) in the A.F. interlamellar crevices, 30-50 nm leaf-like particles were present (Fig. 6); d) in the N.P.Z., 20-30 nm leaf-like particles formed a wide-mesh (Fig. 4). The alcianophylic particle sizes suggest they may correspond to proteoglycan monomers in the A.F. lamellae and mostly proteoglycan aggregates in A.F. interlamellar crevices and N.P.. Both alcianophylia degrees at MgCl2 CEC solutions and enzymatic susceptibility indicate the presence of chondroitin sulphate and keratan sulphate and that the large particles in the A.F. interlamellar crevices are the keratan sulphate richest proteoglycans. The features of the observed AB-proteoglycan particles are consistent with previous morphological data reported for other tissues as well as some biochemical data for the intervertebral disc and may be correlated to the composite mechanical properties of this tissue.","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14351281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA sensitivity to S1 nuclease detected by base-specific fluorochromes. 碱基特异性荧光染料检测DNA对S1核酸酶的敏感性。

Basic and applied histochemistry Pub Date : 1988-01-01

M C Giangarè, E Prosperi, G Bottiroli

引用次数: 0

Extrahypothalamic distribution of vasotocin-immunoreactive fibers and perikarya in the avian central nervous system. 血管催产素免疫反应纤维在鸟类中枢神经系统的下丘脑外分布及核周。

Basic and applied histochemistry Pub Date : 1988-01-01

G C Panzica, M Calcagni, G Ramieri, C Viglietti-Panzica

引用次数: 0

Distribution and development of substance P immunoreactive axons in the chick cornea and uvea. 鸡角膜和葡萄膜中P物质免疫反应轴突的分布和发育。

Basic and applied histochemistry Pub Date : 1988-01-01

G Corvetti, P Pignocchino, L Sisto Daneo

引用次数: 0

Medullary and mesencephalic neuronal groups reacting to antibodies against VIP, somatostatin and bombesin in adult Gallus gallus domesticus. 成年家鸡髓质和中脑神经元群对VIP、生长抑素和bombesin抗体的反应。

Basic and applied histochemistry Pub Date : 1988-01-01

G Nicolardi, G Ambrosi, A Rizzi, M E Camosso

{"title":"Medullary and mesencephalic neuronal groups reacting to antibodies against VIP, somatostatin and bombesin in adult Gallus gallus domesticus.","authors":"G Nicolardi, G Ambrosi, A Rizzi, M E Camosso","doi":"","DOIUrl":"","url":null,"abstract":"The caudo-cranially intermediate one-third of medullary dorsal region, the periaqueductal grey and the rostro-ventral portion of the midbrain tegmentum of adult chickens were studied in detail by means of the PAP-DAB procedure, to define further the main morphological features of the neuronal populations that in previous studies had shown VIP (Vasoactive Intestinal Polypeptide),-Somatostatin (SRIF)-, and Bombesin-like immunoreactivities. In the medulla, VIP-like immunoreactivity was detected within neuronal bodies and processes and extended down to the cervical spinal cord. SRIF-like immunoreactivity was seen only within nerve cell processes, at least a part of which could be sensitive fibre terminals. Bombesin-like immunoreactivity was observed only within neuronal processes. In the periaqueductal grey, all 3 immunoreactivities were detected within perikarya and neuronal processes, with a higher density cranially. In the rostro-ventral portion of the midbrain tegmentum, VIP-like and Bombesin-like immunoreactivities were detected (the latter being located somewhat more cranially) both in neuronal bodies and in processes. SRIF-like immunoreactivity was found in this region only in long neuronal processes.","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14034949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Energy dispersive X-ray microanalysis of sulfated glycosaminoglycans in cartilage matrix stained with alcian blue 8GX. 阿利新蓝8GX染色软骨基质中磺化糖胺聚糖的能量色散x射线微分析。

Basic and applied histochemistry Pub Date : 1988-01-01

L Módis, G Lustyik, R Adány, I Nagy

{"title":"Energy dispersive X-ray microanalysis of sulfated glycosaminoglycans in cartilage matrix stained with alcian blue 8GX.","authors":"L Módis, G Lustyik, R Adány, I Nagy","doi":"","DOIUrl":"","url":null,"abstract":"X-ray spectra were recorded from 400-700 nm matrix areas of 0.5 micron sections prepared from the articular cartilages of 15- and 23-year-old human cadavers. The X-ray microanalysis was carried out (i) on untreated material; (ii) after removing sulfate group by a methylation procedure; (iii) after staining with a copper containing cationic phatolcyanin dye, alcian blue 8GX, preceded by carboxymethylation. K alpha peaks of sulphur could be detected in methylated (i.e. desulfated) samples. These peaks probably indicated the presence of sulphur-containing amino acids in different matrix proteins. Consequently, the measurements of sulphur despite its general use cannot be recommended for the X-ray microanalysis of sulfated glycosaminoglycans of cartilage matrix. K alpha peaks of copper could be identified after carboxymethylation and staining with alcian blue. After carboxymethylation, alcian blue can only be bound to the dissociated sulfate groups of glycosaminoglycans in the cartilage matrix. According to our spectrophotometric studies, approximately one molecule of alcian blue combined with one sulfate group. These data suggested that this technique could be used for semiquantitative estimation of sulfated glycosaminoglycans in small areas of the cartilage matrix. Using this method, we found a higher occurrence of sulfated glycosaminoglycans in the territorial matrix than in the interterritorial matrix of the intermediate and deep zones of the human articular cartilage.","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13613143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chick heart peptidergic innervation: localization and development. 鸡心脏肽能神经的定位与发育。

Basic and applied histochemistry Pub Date : 1988-01-01

G Corvetti, L Andreotti, L Sisto Daneo

引用次数: 0

The LH-RH system of the male European starling: photoperiod induces changes to a possible multifunctional peptide system. 雄性欧椋鸟的rh - rh系统:光周期诱导一个可能的多功能肽系统的变化。

Basic and applied histochemistry Pub Date : 1988-01-01

R G Foster, G Plowman, A R Goldsmith, B K Follett, D M Parry

引用次数: 0

Distribution of substance P immunoreactive cell bodies and fibers in cranial sensory and autonomic ganglia of the chick. 鸡颅感觉神经节和自主神经节P物质免疫反应细胞体和纤维的分布。

Basic and applied histochemistry Pub Date : 1988-01-01

E Strobbia, G Corvetti, L Sisto Daneo