透射电镜凝集素-胶体金技术检测人宫颈上皮黏液糖结合物。月经周期中的定量研究。

Basic and applied histochemistry Pub Date : 1988-01-01
P Versura, M C Maltarello, S Costa, M G Poggi, R Laschi
{"title":"透射电镜凝集素-胶体金技术检测人宫颈上皮黏液糖结合物。月经周期中的定量研究。","authors":"P Versura,&nbsp;M C Maltarello,&nbsp;S Costa,&nbsp;M G Poggi,&nbsp;R Laschi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>We investigated the presence and distribution of five glycosidic residues at the level of the endocervical columnar epithelial cells during the various phases of the menstrual cycle in healthy subjects. We utilized the lectins Wheat Germ Agglutinin (WGA), Peanut Agglutinin (PNA), Soybean Agglutinin (SBA), Concanavalin A (ConA) conjugated with colloidal gold particles as ultrastructural markers in Transmission Electron Microscopy. This technique proved to be particularly suitable for detecting glycosidic residues at the cellular level, where the mucus is produced. All the lectin-binding sites (except ConA binding sites which were not detected) appeared to be produced in significantly different amount during the cycle, supporting the hypothesis that the cyclic-related changes of the cervical mucus viscoelasticity is due to glycoconjugate cyclic variations.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of mucus glycoconjugates in human cervical epithelium by lectin-colloidal gold technique in transmission electron microscopy. I. A quantitative study during the menstrual cycle.\",\"authors\":\"P Versura,&nbsp;M C Maltarello,&nbsp;S Costa,&nbsp;M G Poggi,&nbsp;R Laschi\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We investigated the presence and distribution of five glycosidic residues at the level of the endocervical columnar epithelial cells during the various phases of the menstrual cycle in healthy subjects. We utilized the lectins Wheat Germ Agglutinin (WGA), Peanut Agglutinin (PNA), Soybean Agglutinin (SBA), Concanavalin A (ConA) conjugated with colloidal gold particles as ultrastructural markers in Transmission Electron Microscopy. This technique proved to be particularly suitable for detecting glycosidic residues at the cellular level, where the mucus is produced. All the lectin-binding sites (except ConA binding sites which were not detected) appeared to be produced in significantly different amount during the cycle, supporting the hypothesis that the cyclic-related changes of the cervical mucus viscoelasticity is due to glycoconjugate cyclic variations.</p>\",\"PeriodicalId\":8726,\"journal\":{\"name\":\"Basic and applied histochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Basic and applied histochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Basic and applied histochemistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

我们研究了健康受试者在月经周期不同阶段宫颈内柱状上皮细胞水平上五种糖苷残基的存在和分布。利用小麦胚芽凝集素(WGA)、花生凝集素(PNA)、大豆凝集素(SBA)、豆豆蛋白A (ConA)等凝集素与胶体金粒子偶联作为透射电镜超微结构标记物。这种技术被证明特别适合于检测黏液产生的细胞水平的糖苷残基。所有凝集素结合位点(除了未检测到的ConA结合位点)在周期中产生的量似乎都有显著差异,支持了宫颈黏液粘弹性的周期相关变化是由于糖结合周期变化引起的假设。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of mucus glycoconjugates in human cervical epithelium by lectin-colloidal gold technique in transmission electron microscopy. I. A quantitative study during the menstrual cycle.

We investigated the presence and distribution of five glycosidic residues at the level of the endocervical columnar epithelial cells during the various phases of the menstrual cycle in healthy subjects. We utilized the lectins Wheat Germ Agglutinin (WGA), Peanut Agglutinin (PNA), Soybean Agglutinin (SBA), Concanavalin A (ConA) conjugated with colloidal gold particles as ultrastructural markers in Transmission Electron Microscopy. This technique proved to be particularly suitable for detecting glycosidic residues at the cellular level, where the mucus is produced. All the lectin-binding sites (except ConA binding sites which were not detected) appeared to be produced in significantly different amount during the cycle, supporting the hypothesis that the cyclic-related changes of the cervical mucus viscoelasticity is due to glycoconjugate cyclic variations.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信