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Basic and applied histochemistry最新文献

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Histamine uptake by human normal neutrophils in vitro. 体外正常中性粒细胞对组胺的摄取。
Basic and applied histochemistry Pub Date : 1990-01-01
C Catini, A Miliani, G Gheri, M Legnaioli
{"title":"Histamine uptake by human normal neutrophils in vitro.","authors":"C Catini,&nbsp;A Miliani,&nbsp;G Gheri,&nbsp;M Legnaioli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The uptake of different concentrations of (2.5-3H) histamine by normal human neutrophils at 37.5 degrees C in a tris-albumin buffer was determined at 20, 60 and 90 min of incubation. Uptake was correlated with the concentration of amine and incubation time with a saturation-like curve. On the basis of these results, it is suggested that neutrophils play a role in the regulation of histamine plasma levels.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13418618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hinf I restriction endonuclease digestion on human fixed metaphase chromosomes. 人类固定中期染色体上的hinif限制性内切酶酶切。
Basic and applied histochemistry Pub Date : 1990-01-01
L Stuppia, P Guanciali Franchi, G Calabrese, G Parruti, G Palka, U Bianchi
{"title":"Hinf I restriction endonuclease digestion on human fixed metaphase chromosomes.","authors":"L Stuppia,&nbsp;P Guanciali Franchi,&nbsp;G Calabrese,&nbsp;G Parruti,&nbsp;G Palka,&nbsp;U Bianchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors report on the activity of Hinf I restriction endonuclease on human fixed metaphase chromosomes. Experiments performed by digesting chromosomes just after harvesting or after ageing in methanol-acetic acid displayed a different pattern of digestion on metaphases, since only aged preparations showed gaps on heterochromatic regions of chromosomes 1, 9 and 16 and C-like bands on other chromosomes. In this view, the authors suggest that structural modifications of the DNA, induced by acid fixation, can influence Hinf I activity on fixed metaphase chromosomes.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13374832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Langerhans cells in odontogenic tumours and cysts as detected by S-100 protein immunohistochemistry. S-100蛋白免疫组化检测牙源性肿瘤和囊肿中的朗格汉斯细胞。
Basic and applied histochemistry Pub Date : 1990-01-01
N Murase, Y Tatemoto, Y Iwai, Y Okada, M Mori
{"title":"Langerhans cells in odontogenic tumours and cysts as detected by S-100 protein immunohistochemistry.","authors":"N Murase,&nbsp;Y Tatemoto,&nbsp;Y Iwai,&nbsp;Y Okada,&nbsp;M Mori","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immunohistochemical demonstration of S-100 protein in Langerhans cells (LCs) was made in odontogenic epithelial tumours (71 cases), radicular cysts (40 cases), follicular cysts (28 cases), odontogenic keratocysts (11 cases), primordial cysts (7 cases) and fissual cysts (6 cases). With the use of polyclonal antiserum against S-100 protein, positive LCs, dendrical or irregular in shape were found in tumour or cystic epithelia, and sometimes in stromal connective tissue. Incidence of positive S-100 staining LCs was 11 cases out of 61 ameloblastomas, 22 cases out of 40 radicular cysts, 3 cases of 28 follicular cysts, and other lesions in both odontogenic tumours and cystic diseases lacked LCs. The cases with S-100 protein positive LCs were usually accompanied with a high degree of inflammatory infiltration in their lesions; on the contrary, the negative cases also generally lacked inflammatory responses.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13374834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-specific binding of immunoglobulins and protein A-Gold complex with cytoplasmic granules of human mast cells. 免疫球蛋白和蛋白a -金复合物与人肥大细胞细胞质颗粒的非特异性结合。
Basic and applied histochemistry Pub Date : 1990-01-01
A Onetti Muda, A Crescenzi, T Faraggiana, V Marinozzi
{"title":"Non-specific binding of immunoglobulins and protein A-Gold complex with cytoplasmic granules of human mast cells.","authors":"A Onetti Muda,&nbsp;A Crescenzi,&nbsp;T Faraggiana,&nbsp;V Marinozzi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A non-specific staining of mast cells by a number of proteins and immunoglobulins has been observed using different immunocytochemical techniques and different antisera at light and electron microscopy level. This finding has been related to an ionic binding of cationic charges, such as aminic groups with an high pI, to the strongly anionic residues of mast cell granules. This hypothesis is supported by the lack of non-specific binding achieved using negatively charged chromogens (i.e. FITC--or Rhodamine-conjugated antibodies) or using cationic dyes before the immunohistochemical reaction. It is therefore stressed that adequate and accurate negative controls must always be carried out in order to get correct interpretations of the staining results.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13418619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isobestic wavelength determination for succinic dehydrogenase-nitroblue tetrazolium-derived formazans in autonomic neurons. 自主神经元中琥珀酸脱氢酶-硝基蓝四氮唑衍生的甲醛酰胺的等致波长测定。
Basic and applied histochemistry Pub Date : 1990-01-01
D M Baker, R M Santer
{"title":"Isobestic wavelength determination for succinic dehydrogenase-nitroblue tetrazolium-derived formazans in autonomic neurons.","authors":"D M Baker,&nbsp;R M Santer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two methods have been used to determine the isobestic (equiconcentration) wavelength of formazans derived from nitroblue tetrazolium-succinate dehydrogenase (EC 1.3.99.1) activity in cryostat sections of the rat superior cervical ganglion prior to microdensitometric measurements. Both methods indicate that maximal absorbance of the final reaction product is at a wavelength of 540-550 nm. This wavelength differs by 35-45 nm from that used to measure the same reaction product in sections of other rat tissues such as liver and implies that it may be unwise to adopt a \"standard\" wavelength for a particular reaction product when making microdensitometric measurements in relation to quantitative enzyme histochemistry.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13487178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LAK1: a novel leucocyte differentiation antigen shared by lymphoid and endothelial cells. LAK1:淋巴细胞和内皮细胞共有的一种新的白细胞分化抗原。
Basic and applied histochemistry Pub Date : 1990-01-01
M R Zocchi, A Faravelli, E Gianazza, R Pardi, C Rugarli
{"title":"LAK1: a novel leucocyte differentiation antigen shared by lymphoid and endothelial cells.","authors":"M R Zocchi,&nbsp;A Faravelli,&nbsp;E Gianazza,&nbsp;R Pardi,&nbsp;C Rugarli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Lymphokine activated killer (LAK) cells have been utilized as a useful tool in cancer adoptive immunotherapy. The lineage origin of this population has always been controversial since it shares phenotypic markers with both myelomonocytic cells and T lymphocytes. Recently we described a new monoclonal antibody (MoAb), termed LAK1, which recognizes a 120 Kd surface molecule expressed on human large granular lymphocytes (LGL) and LAK precursors and effectors. LAK1 MoAb defines two different populations of positive cells amongst peripheral lymphocytes: the first subset (20%), represented by brightly stained cells, belongs to the non T-LGL population, whereas the second subset (30%) displays low fluorescence intensity and was partially composed of T lymphocytes. More interestingly, LAK1 is shared by some other cell types, such as monocytes and vascular endothelial cells. Immunohistochemical staining performed on muscle, endometrium and lymphoid or other non lymphoid tissues shows that LAK1 antigen is selectively expressed by the reticuloendothelial system.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13487179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Substance P- and calcitonin gene-related peptide-like immunoreactive pericellular baskets in human trigeminal ganglion. 人三叉神经节P物质和降钙素基因相关肽样免疫反应性细胞周篮。
Basic and applied histochemistry Pub Date : 1990-01-01
M Quartu, A Floris, M Del Fiacco
{"title":"Substance P- and calcitonin gene-related peptide-like immunoreactive pericellular baskets in human trigeminal ganglion.","authors":"M Quartu,&nbsp;A Floris,&nbsp;M Del Fiacco","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Presence of pericellular basket-like network of fibres immunoreactive to substance P and to calcitonin gene-related peptide is shown around non-immunoreactive primary sensory neurons of human trigeminal ganglion. Single neurons can be wrapped by either peptide-containing fibres or by both. Such pericellular structures might represent sites of interneuronal communications at ganglionic level.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12866955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenylfluorone, a new staining agent with higher selectivity for tin. 对锡具有较高选择性的新型染色剂苯基氟酮。
Basic and applied histochemistry Pub Date : 1990-01-01
H R Fukuoka
{"title":"Phenylfluorone, a new staining agent with higher selectivity for tin.","authors":"H R Fukuoka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to search for a new staining agent with higher selectivity for particular metals, the ability of fluorone derivatives to histochemically stain metals present in rat tissues was examined. Among a variety of metals tested, phenylfluorone showed intense staining only for tin. The phenylfluorone method was superior to the conventional gallein method with regard to selectivity when staining for tin among various metals in histochemical practice.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12866956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hemoglobin stain in fixed erythroleukemic cells: an improvement of the method combined with autoradiography. 固定红细胞的血红蛋白染色:结合放射自显影法的改进。
Basic and applied histochemistry Pub Date : 1990-01-01
M G Raffa, G Serenelli, M P Viola Magni
{"title":"Hemoglobin stain in fixed erythroleukemic cells: an improvement of the method combined with autoradiography.","authors":"M G Raffa,&nbsp;G Serenelli,&nbsp;M P Viola Magni","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12866957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Different distribution of thiaminpyrophosphatase activity in neuronal and glial cell enriched fractions from human and rat brain: an isoelectric focusing investigation. 硫胺焦磷酸酶活性在人脑和大鼠脑神经元和胶质细胞富集组分中的不同分布:等电聚焦研究。
Basic and applied histochemistry Pub Date : 1990-01-01
U Laforenza, P Mazzarello, C Patrini, M Poloni, G P Casadei, G Rindi
{"title":"Different distribution of thiaminpyrophosphatase activity in neuronal and glial cell enriched fractions from human and rat brain: an isoelectric focusing investigation.","authors":"U Laforenza,&nbsp;P Mazzarello,&nbsp;C Patrini,&nbsp;M Poloni,&nbsp;G P Casadei,&nbsp;G Rindi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Enriched fractions of neuronal and glial cells from rat and human (autoptical specimens of frontal cortex) brains were assayed for thiaminpyrophosphatase (TPPase) activity by isoelectric focusing (IEF). Glial and neuronal fractions yielded about 0.16 and 0.03 g/g of wet tissue respectively. Purity was estimated to be 85-90%. The isolated fractions were homogenized in the presence of 1% Triton X-100, and IEF was carried out on thin layer polyacrylamide gel in a pH range of 3.5-9.5, using Ampholine PAG plates. TPPase activity of the protein bands was assayed by laser-densitometry, after incubation with thiaminpyrophosphate in an appropriate buffer and PbS staining. IEF analysis showed that TPPase activity was present almost exclusively in the neuronal fraction (at levels 16-fold higher than those found in the glial fraction). After IEF, TPPase activity was present in 10 distinct protein bands with different isoelectric points. These preliminary data suggest that TPPase may be involved in the neuronal activity of rat and human brain.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13324670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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