Basic and applied histochemistry最新文献_第2页

Analysis of GFA-protein mRNA expression in developing bovine brain by in situ hybridization and northern blot hybridization. 原位杂交和northern blot杂交分析发育中的牛脑gfa蛋白mRNA的表达。

Basic and applied histochemistry Pub Date : 1990-01-01

K Nakanishi, T Kitamura, M Okuda, T Mazaki, S Watanabe, N Miyoshi, S Fujita, M Fukuda

{"title":"Analysis of GFA-protein mRNA expression in developing bovine brain by in situ hybridization and northern blot hybridization.","authors":"K Nakanishi, T Kitamura, M Okuda, T Mazaki, S Watanabe, N Miyoshi, S Fujita, M Fukuda","doi":"","DOIUrl":"","url":null,"abstract":"Using bovine brains of adult and developmental stages, the time and place of the appearance of mRNA for glial fibrillary acidic protein (GFA-protein) were studied by in situ- and Northern blot-hybridization. Double-stranded cDNA labeled with [3H]dCTP or [32P]dCTP was used as the probe for this mRNA. To compare the location of GFA-protein mRNA and GFA-protein itself on serial sections. GFA-protein immunohistochemistry was used. By in situ hybridization with adult bovine brain. GFA-protein mRNA was detected in astroglia, most of which were in the white matter. The distribution of these astroglia by in situ hybridization was consistent with the findings by GFA-protein immunohistochemistry and Northern blot hybridization, indicating that each techniques were specific. Concerning fetal stages, GFA-protein mRNA could be detected in the brain of a fetal calf with a body length of 28 cm by in situ hybridization using the 32P-labeled probe, and the mRNA was localized in the subpial area and the fornix. These results indicated that glial maturation first became recognizable at least in the subpial area and the fornix in the brain of a fetal calf measuring 28 cm. In this fetal brain, GFA-protein mRNA was almost undetectable by Northern blot hybridization. This suggested that in situ hybridization was more sensitive and useful for the analysis of gene expression than Northern blot hybridization, when the target mRNA is present in only a limited area, such as in the brain.","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"34 2","pages":"101-10"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13374831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell kinetics of PHA-activated lymphocytes are slowed by prolonged hypertonic stress. pha活化淋巴细胞的细胞动力学因长时间高渗应激而减慢。

Basic and applied histochemistry Pub Date : 1990-01-01

A M Fuhrman Conti, R Tori, E Ronchetti, L De Grada, C Pellicciari, M G Manfredi Romanini

引用次数: 0

Immunohistochemical localization of filaggrin in benign and malignant lesions of the human oral mucosa. 人口腔黏膜良、恶性病变中聚丝蛋白的免疫组化定位。

Basic and applied histochemistry Pub Date : 1990-01-01

M Grosso, M Lentini, E Bellizi De Marco, R Petracca, P Leoncini, G Carrozza

引用次数: 0

Histochemical studies on irradiated and obstructive human submandibular glands. Protein distribution and lectin-binding in the degenerative glands. 照射和阻塞性人颌下腺的组织化学研究。退行性腺体中的蛋白质分布和凝集素结合。

Basic and applied histochemistry Pub Date : 1990-01-01

Y Tatemoto, J Hirota, K Ryoke, T Osaki

{"title":"Histochemical studies on irradiated and obstructive human submandibular glands. Protein distribution and lectin-binding in the degenerative glands.","authors":"Y Tatemoto, J Hirota, K Ryoke, T Osaki","doi":"","DOIUrl":"","url":null,"abstract":"Immunohistochemical protein distribution of alpha-amylase (Am), lysozyme (Ly), cytokeratinin (CK), S-100 protein (S-100) and secretory component (SC), and lectin-binding (SBA and UEA-I) profiles were studied in 10 obstructive and 20 irradiated human submandibular glands which were surgically extirpated. Degenerative intensity of the glands was graded as I, II and III based on the order of severity. All proteins generally existed in serous acinic cells of the intact glands. The proteins immunoreactivities became weak even in mildly inflamed glands (grade I), and nearly disappeared from the moderately damaged glands (grade II). Duct cells had clear CK and some cells reacted with the anti-SC antibody, but other proteins were not observed on the ducts. Mucous cells possessed none of the proteins, and their lectin-binding was only traceable in some glands. Compared with immunoreactivities in the proteins, lectin-binding profiles were different. SBA and UEA-I bound somewhat similarly to both acinic and duct cells, and the binding was hardly affected even by severe degeneration (grade III). Between obstructive and irradiated glands, no obvious difference was observed in either protein distribution or lectin-binding. From the above, it seems that some proteins are more affective to the degeneration and that lectin-binding sugar residues are non-affective against the degenerative changes of the tissues.","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"34 1","pages":"59-70"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12856899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochemical evaluation of C-heterochromatic-DNA in metaphase chromosomes. 中期染色体c-异色dna的细胞化学评价。

Basic and applied histochemistry Pub Date : 1990-01-01

C Pellicciari, E Ronchetti, R Tori, D Formenti, M G Manfredi Romanini

引用次数: 0

The comparative chemical morphology of the mammalian cornea. 哺乳动物角膜的比较化学形态。

Basic and applied histochemistry Pub Date : 1990-01-01

J E Scott, T R Bosworth

引用次数: 0

Details of the female and male pathway of the Keimbahn determined by enzyme histochemical and autoradiographic studies. 通过酶组织化学和放射自显影研究确定Keimbahn的雌性和雄性通路的细节。

Basic and applied histochemistry Pub Date : 1990-01-01

W Hilscher, B Hilscher

{"title":"Details of the female and male pathway of the Keimbahn determined by enzyme histochemical and autoradiographic studies.","authors":"W Hilscher, B Hilscher","doi":"","DOIUrl":"","url":null,"abstract":"Autoradiographic studies and the use of enzyme histochemistry have revealed that early germ line cells (female and male PGC, oogonia, prediplotene oocytes and prospermatogonia) as well as the more advanced germ cells (diplotene oocytes, spermatogonia, spermatocytes and spermatids) show specific patterns of their DNA and RNA synthesis and their enzymatic equipment. The female and male germ lines show similar kinetics up to the arise of oocytes and T prospermatogonia (T for transitional), the final products of a first limited multiplication process of primitive gonia. In former studies we supposed that oocytes and T prospermatogonia are the first exponents of the female and male pathway of the germ line (Hilscher and Hilscher, 1989a). Recently, it could be shown--using the reverse PLM method in slides of plastic embedded material--that the first differences between female and male GC can already be stated at the end of the first proliferation wave of oogonia and multiplying prospermatogonia; that means even before the existence of oocytes and T prospermatogonia (Hilscher and Hilscher, 1989b). Oogonia and M prospermatogonia (M for multiplying) are equipped both with only one active X chromosome. While oocytes traverse the prediplotene stages of meiotic prophase T prospermatogonia prepare for a second extensive proliferation process: spermatogenesis. Oocytes in meiosis are provided with two active X chromosomes, T prospermatogonia possess only one, and the presence of the Y chromosome is not vital for them. However, the Y chromosome is required for the normal course of spermatogenesis characterized by a stock of stem cells, that are responsible for the continuous production of male gamets. The mammalian oocyte--similar as that of insects and amphibia but to a lower degree--acts as pre-embryo.","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"34 1","pages":"21-34"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13339116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of pancreatic glucagon cells in the cartilaginous fish Scyliorhinus canicula by ultrastructural immunocytochemistry. 超微结构免疫细胞化学方法鉴定软骨鱼胰高血糖素细胞。

Basic and applied histochemistry Pub Date : 1990-01-01

G Faraldi, M Canepa, L Borgiani, T Zanin

引用次数: 0

A geometrical construction to assess scanning technique in microphotometry. 一种评估显微光度学中扫描技术的几何结构。

Basic and applied histochemistry Pub Date : 1990-01-01

K A Chaubal

引用次数: 0

The endocrine pancreas of Triturus cristatus: an immunocytochemical study. 黑麦的内分泌胰腺:免疫细胞化学研究。

Basic and applied histochemistry Pub Date : 1990-01-01

R Putti, L Varano, V Laforgia, A Cavagnuolo

引用次数: 0