pha活化淋巴细胞的细胞动力学因长时间高渗应激而减慢。

Basic and applied histochemistry Pub Date : 1990-01-01
A M Fuhrman Conti, R Tori, E Ronchetti, L De Grada, C Pellicciari, M G Manfredi Romanini
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引用次数: 0

摘要

研究了植物血凝素刺激有丝分裂过程中长时间暴露于高渗培养基对人淋巴细胞的影响。研究了刺激前24小时(G0到G1过渡)染色质去浓缩的过程以及刺激48 ~ 72小时的动力学参数变化和染色体畸变的发生情况。在HT培养基中,淋巴细胞由G0向G1过渡减慢;PHA刺激48 h后,s期细胞数量减少,72 h后耐药细胞的s期细胞数量与对照组相同。有丝分裂指数较小,G0/G1细胞频率较大。染色体畸变频率未见明显增加。这些发现表明,人外周血淋巴细胞可以在高渗培养基中存活和生长;染色体损伤,如果不修复,可能是致命的,只有核型正常的淋巴细胞才能在高温培养基中存活很长时间,尽管其动力学特征有所改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cell kinetics of PHA-activated lymphocytes are slowed by prolonged hypertonic stress.

The effect of prolonged exposure to a hypertonic medium on human lymphocytes during mitogenic stimulation with phytohemagglutinin was investigated. The process of chromatin decondensation during the first 24 hrs stimulation (G0 to G1 transition) and the changes in kinetic parameters and the occurrence of chromosome aberrations from 48 hrs to 72 hrs of stimulation were studied. In HT medium, lymphocyte transition from G0 to G1 was slowed; there were fewer S-phase cells, after 48 hrs PHA stimulation, whereas after 72 hrs the resistant cells showed the same frequency of S-phase cells as the controls. The mitotic index was always smaller, and the frequency of G0/G1 cells larger. No significant increase in the frequencies of chromosome aberrations were found. These findings suggest that human peripheral lymphocytes can survive and grow in a hypertonic medium; chromosome damages, if not repaired, may be lethal, and only lymphocytes with normal karyotypes can survive for long times in the HT medium, although with modified kinetic characteristics.

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