Histochemical studies on irradiated and obstructive human submandibular glands. Protein distribution and lectin-binding in the degenerative glands.

Basic and applied histochemistry Pub Date : 1990-01-01
Y Tatemoto, J Hirota, K Ryoke, T Osaki
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Abstract

Immunohistochemical protein distribution of alpha-amylase (Am), lysozyme (Ly), cytokeratinin (CK), S-100 protein (S-100) and secretory component (SC), and lectin-binding (SBA and UEA-I) profiles were studied in 10 obstructive and 20 irradiated human submandibular glands which were surgically extirpated. Degenerative intensity of the glands was graded as I, II and III based on the order of severity. All proteins generally existed in serous acinic cells of the intact glands. The proteins immunoreactivities became weak even in mildly inflamed glands (grade I), and nearly disappeared from the moderately damaged glands (grade II). Duct cells had clear CK and some cells reacted with the anti-SC antibody, but other proteins were not observed on the ducts. Mucous cells possessed none of the proteins, and their lectin-binding was only traceable in some glands. Compared with immunoreactivities in the proteins, lectin-binding profiles were different. SBA and UEA-I bound somewhat similarly to both acinic and duct cells, and the binding was hardly affected even by severe degeneration (grade III). Between obstructive and irradiated glands, no obvious difference was observed in either protein distribution or lectin-binding. From the above, it seems that some proteins are more affective to the degeneration and that lectin-binding sugar residues are non-affective against the degenerative changes of the tissues.

照射和阻塞性人颌下腺的组织化学研究。退行性腺体中的蛋白质分布和凝集素结合。
本文研究了手术切除的10个梗阻性颌下腺和20个照射后颌下腺中α -淀粉酶(Am)、溶菌酶(Ly)、细胞角化素(CK)、S-100蛋白(S-100)和分泌成分(SC)的免疫组化蛋白分布以及凝集素结合(SBA和UEA-I)谱。腺体退行性程度按严重程度分为I、II和III级。这些蛋白普遍存在于完整腺体的浆液腺泡细胞中。即使在轻度炎症的腺体(I级)中,蛋白质的免疫反应活性也变弱,在中度损伤的腺体(II级)中几乎消失。导管细胞有清晰的CK,一些细胞与抗sc抗体反应,但在导管上未观察到其他蛋白质。粘液细胞不具有这些蛋白质,它们的凝集素结合仅在某些腺体中可追踪到。与免疫反应性比较,凝集素结合谱不同。SBA和UEA-I与腺泡细胞和导管细胞的结合有些相似,即使严重变性(III级),其结合也几乎不受影响。在梗阻性和辐照性腺之间,蛋白质分布和凝集素结合均无明显差异。综上所述,似乎有些蛋白质对组织的退行性变化更有影响,而凝集素结合糖残基对组织的退行性变化没有影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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