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Basic and applied histochemistry最新文献

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Chromogranin A as a marker of neuroendocrine histogenesis of tumours: an immunoelectron microscopic study with considerations about the influence of fixation and embedding media on immunolabelling. 嗜铬粒蛋白A作为肿瘤神经内分泌组织发生的标志物:考虑固定和包埋介质对免疫标记影响的免疫电镜研究。
Basic and applied histochemistry Pub Date : 1990-01-01
B E Leone, G L Taccagni, G Dell'Antonio, A Cantaboni
{"title":"Chromogranin A as a marker of neuroendocrine histogenesis of tumours: an immunoelectron microscopic study with considerations about the influence of fixation and embedding media on immunolabelling.","authors":"B E Leone,&nbsp;G L Taccagni,&nbsp;G Dell'Antonio,&nbsp;A Cantaboni","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ultrastructural localization of chromogranin A (Chr A) was studied in eleven neoplasias of the diffuse neuroendocrine system (3 pancreatic islet-cell tumours, 1 medullary carcinoma of the thyroid, 1 large bowel and 1 small bowel carcinoid tumours, 2 carcinoid tumours of the lung, 1 adenoma of the parathyroid gland, 2 pheochromocytomas of the adrenal gland). On account of the great influence of the technical treatment of the samples on the immunolocalization of Chr A, the effect of the following variables was studied in a case of pheochromocytoma: fixation in glutaraldehyde versus paraformaldehyde, postfixation in osmium tetroxide versus omission, embedding in epoxy resin versus acrylic resin. The method of choice for the better preservation of the antigenic character of the tissue was found to be fixation in 4% paraformaldehyde, omission of osmium postfixation and embedding in LRWhite acrylic resin; by this procedure we were able to find Chr A in the neurosecretory granules of all the studied cases, using three commercially available antibodies directed against Chr A. These findings further confirm that Chr A is a reliable marker for the study of neuroendocrine neoplasias by electron microscopy.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13374835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved methods for the determination of 5-bromo-2-deoxyuridine labelling index in clones and colonies growing in plasma clots. 血浆凝块中5-溴-2-脱氧尿苷标记指数测定方法的改进。
Basic and applied histochemistry Pub Date : 1990-01-01
G D Di Nucci, P L Tazzari, A Bontadini, C Tassi, A Dinota, S Pileri, M Gobbi
{"title":"Improved methods for the determination of 5-bromo-2-deoxyuridine labelling index in clones and colonies growing in plasma clots.","authors":"G D Di Nucci,&nbsp;P L Tazzari,&nbsp;A Bontadini,&nbsp;C Tassi,&nbsp;A Dinota,&nbsp;S Pileri,&nbsp;M Gobbi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bromodeoxyuridine (BrdUrd), an analogue of thymidine is one of the most employed marker to detect the S-phase of the cell cycle. Difficulties are described for the in situ detection of S-phase cells in normal and neoplastic growing clones. In this paper we propose new methods for the detection of BrdUrd in neoplastic clones, growing in plasma clots. In particular, these methods are based on the immunocytochemical staining with horseradish peroxidase and alkaline phosphatase, as well as on a new immunofluorescent streptavidin technique. They allow easy detection of S-phase cells with an inverted light microscope.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13487780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of the endocrine cells detected by the monoclonal antibody HISL-19 in human tissues. 单克隆抗体HISL-19检测人组织内分泌细胞的鉴定。
Basic and applied histochemistry Pub Date : 1990-01-01
R Buffa, M Pelagi, A G Siccardi, M Curzio, J Y Yu, C Bordi
{"title":"Identification of the endocrine cells detected by the monoclonal antibody HISL-19 in human tissues.","authors":"R Buffa,&nbsp;M Pelagi,&nbsp;A G Siccardi,&nbsp;M Curzio,&nbsp;J Y Yu,&nbsp;C Bordi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The human endocrine cells reacting with the monoclonal antibody HISL-19 were identified with hormone antisera of proven specificity using a double immunostaining procedure. The epitope for HISL-19 was found in all types of pituitary cells except ACTH cells, in thyroid C cells, in all types of adrenal medullary and pancreatic islet cells and in somatostatin and pancreatic polypeptide cells of the gastrointestinal mucosa. No staining was found in parathyroid cells and in most gastrointestinal endocrine cells. Either paranuclear focal accumulation or diffuse cytoplasmic distribution of immunoreactive material were found. The spectrum of HISL-19 immunoreactive cells was found to be only in part complementary to that of cells immunoreactive for chromogranin A. Thus, it is concluded that the monoclonal antibody HISL-19 is a useful addition to other immunohistochemical markers for the detection of cells showing neuroendocrine features.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12869766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thiamine, thiamine phosphates and thiamine metabolizing enzymes in synaptosomes of rat brain. 大鼠脑突触体中硫胺素、硫胺素磷酸和硫胺素代谢酶的研究。
Basic and applied histochemistry Pub Date : 1990-01-01
U Laforenza, C Patrini, P Mazzarello, M Poloni, G Rindi
{"title":"Thiamine, thiamine phosphates and thiamine metabolizing enzymes in synaptosomes of rat brain.","authors":"U Laforenza,&nbsp;C Patrini,&nbsp;P Mazzarello,&nbsp;M Poloni,&nbsp;G Rindi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Thiamine and thiamine mono-, pyro- and triphosphate were found at detectable levels in synaptosomes isolated from whole rat brain. Synaptosomes prepared from whole brain, cerebellum and medulla were also found to contain uridine and inosine mono- and diphosphatases as well as the thiamine pyrophosphate synthetizing and hydrolyzing enzymes, but no thiamine monophosphatase. By isoelectric focusing on thin layer polyacrylamide gel of Triton X-100 homogenates of synaptosomes, thiamine pyrophosphatase activity could be separated into 10 bands with different isoelectric points. The contents of thiamine compounds and enzymes in synaptosomes were generally lower than those found in neuronal cell bodies.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13121691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The coelomocytes of Holothuria polii (Echinodermata). II. Cytochemical staining properties. 棘皮Holothuria polii的体腔细胞。2细胞化学染色特性。
Basic and applied histochemistry Pub Date : 1990-01-01
G D'Ancona, C Canicattì
{"title":"The coelomocytes of Holothuria polii (Echinodermata). II. Cytochemical staining properties.","authors":"G D'Ancona,&nbsp;C Canicattì","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Histochemical studies by traditional staining methods for lipids, mucopolysaccharides and proteins and histoenzymatic reactions typical of lysosomal activity were carried out on the principal coelomocyte types of Holothuria polii. These are considered to be the cellular contigent of the immune response, and in Holothuria polii, they probably take part also in clotting events.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13302342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lectin binding to the egg envelopes in Eyprepocnemis plorans (Charp.) (Orthoptera, Acrididae). 卵包膜上凝集素的结合(直翅目，蝗科)。
Basic and applied histochemistry Pub Date : 1990-01-01
G Longo, R Viscuso, L Sottile, A Giuffrida
{"title":"Lectin binding to the egg envelopes in Eyprepocnemis plorans (Charp.) (Orthoptera, Acrididae).","authors":"G Longo,&nbsp;R Viscuso,&nbsp;L Sottile,&nbsp;A Giuffrida","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The distribution of glycoconjugates in the egg envelopes of Eyprepocnemis plorans was investigated using various FITC-conjugated lectins. In the epichorion, the lectins ConA, SBA and WGA each have particular binding patterns, while TPA binding is confined to its deepest regions only. The glycoconjugates of the micropylar wall present different characteristics from those of the surrounding chorion. The vitelline coat shows a marked binding for WGA and TPA only; below the inner micropylar openings, this binding pattern is uniform over the whole extent of the coat and therefore it is not possible to identify specific binding sites for these two lectins. Contrary to what has been observed in some other insect species, the vitelline coat does not seem to be involved in the structural organization of the mycropyles.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13374833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Absorbance scanning and systematic error. 吸光度扫描和系统误差。
Basic and applied histochemistry Pub Date : 1990-01-01
M Van der Ploeg, W A Duijndam
{"title":"Absorbance scanning and systematic error.","authors":"M Van der Ploeg,&nbsp;W A Duijndam","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13442458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrated dystrophin analysis using immunocytochemical, biochemical and genetic techniques. 利用免疫细胞化学、生化和遗传技术综合分析肌营养不良蛋白。
Basic and applied histochemistry Pub Date : 1990-01-01
L V Nicholson, M A Johnson, K E Davies
{"title":"Integrated dystrophin analysis using immunocytochemical, biochemical and genetic techniques.","authors":"L V Nicholson,&nbsp;M A Johnson,&nbsp;K E Davies","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immunocytochemistry is a powerful tool for the elucidation of clinical problems, particularly when used in conjunction with complementary techniques such as immunoblotting and DNA analysis. We would like to present an experimental example which illustrates the way these three different disciplines were brought together to investigate the defective gene product in X-linked muscular dystrophy.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12866954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chromatin changes in frog neurons after eighth nerve transection. 第八次神经横断后青蛙神经元的染色质变化。
Basic and applied histochemistry Pub Date : 1989-01-01
G Bernocchi, S Giacometti, E Scherini, P Valli
{"title":"Chromatin changes in frog neurons after eighth nerve transection.","authors":"G Bernocchi,&nbsp;S Giacometti,&nbsp;E Scherini,&nbsp;P Valli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fourteen days after unilateral eighth nerve transection in the frog, Purkinje neurons of the lobus vestibulolateralis and corpus of the cerebellum and medium-sized neurons of the vestibular nuclear complex showed changes in metabolic activity. In the ipsilateral parts, and to a lesser extent in the contralateral parts, of operated frogs, the Feulgen-DNA values were higher and the nuclear areas larger, associated with decondensation of chromatin. The cytoplasmic basophilia was also less. These changes could be due to anabolic responses of the neuronal populations during regeneration. The anabolic reaction of the corpus cerebelli and contralateral vestibular nuclear complex is only partially non-specific and ascribable to the surgical trauma (comparison between sham-operated and unoperated frogs). The results indicate clear patterns of connection between the ipsilateral and contralateral parts and between the cerebellar and vestibular nuclear complex neurons.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Congenital muscle fibre type disproportion: clinical, morphological and biochemical findings in children. 先天性肌纤维类型失调:儿童的临床、形态和生化表现。
Basic and applied histochemistry Pub Date : 1989-01-01
O Pastoris, M Dossena, L Vercesi, R Scelsi, M Grana, E Bianchi
{"title":"Congenital muscle fibre type disproportion: clinical, morphological and biochemical findings in children.","authors":"O Pastoris,&nbsp;M Dossena,&nbsp;L Vercesi,&nbsp;R Scelsi,&nbsp;M Grana,&nbsp;E Bianchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Muscle biopsies from quadriceps femoris muscle of normal subjects and subjects with symptoms of congenital myopathy, aged 1-3 years, were examined for morphological and biochemical differences. Four patients showed clinical signs of Congenital Fibre Type Disproportion (CFTD) as described originally by Brooke (1973), while the other cases showed only signs of hypotonia and diffuse weakness as described by Clancy et al. (1980). No morphological differences between patients with different clinical signs were found in any biopsy sample from the quadriceps femoris muscle, as regards fibre size; type I fibres were always smaller than type II fibres. The difference in fibre size was more than 15% in all cases, and the variability coefficient of the larger type II fibres was less than 250. Nevertheless, as regards fibre occurrence, two patients showed more type I fibres than type II fibres, four patients showed more type II fibres than type I fibres and one patient had a marked type II fibre predominance. Enzyme activities assayed in the crude mitochondrial fraction showed no abnormalities between normal subjects and patients. An increase in the activity of lactate dehydrogenase in the crude extract was found in all cases.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13896067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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