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Basic and applied histochemistry最新文献

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Cytochemical localization of alkaline phosphatase and Na+, K+-ATPase activities in the blood-brain barrier of Rana esculenta. 血脑屏障中碱性磷酸酶和Na+、K+- atp酶活性的细胞化学定位。
Basic and applied histochemistry Pub Date : 1989-01-01
M Lazzari, V Franceschini, F Ciani, G Minelli
{"title":"Cytochemical localization of alkaline phosphatase and Na+, K+-ATPase activities in the blood-brain barrier of Rana esculenta.","authors":"M Lazzari,&nbsp;V Franceschini,&nbsp;F Ciani,&nbsp;G Minelli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ultrastructural distribution of alkaline phosphatase and Na+, K+-ATPase on the brain capillaries in Rana esculenta was investigated. Alkaline phosphatase activity appears both on the luminal and abluminal walls of the endothelial capillary cells; Na+, K+-ATPase is, instead, only present on the abluminal side. This different enzymatic distribution indicates that endothelial cells of the brain capillaries are polarized and the luminal and abluminal endothelial membranes are functionally different. The role of these two enzymatic activities is discussed in relation to the blood-brain barrier.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13690903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alkaline phosphatase cytochemistry in confocal scanning light microscopy for imaging the bone marrow stroma. 碱性磷酸酶细胞化学在共聚焦扫描光显微镜成像骨髓基质。
Basic and applied histochemistry Pub Date : 1989-01-01
P Bianco, A Boyde
{"title":"Alkaline phosphatase cytochemistry in confocal scanning light microscopy for imaging the bone marrow stroma.","authors":"P Bianco,&nbsp;A Boyde","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper reports on the use of alkaline phosphatase cytochemistry and combined conventional and confocal reflection and fluorescence scanning light microscopic modes in the study of human marrow stroma. It was found that the end product of the enzyme reaction using Napthol AS phosphate as substrate and Fast Blue BB as coupler reflected the 633 nm (red) light from a Helium-Neon laser. Serial optical sections suitable for 3-D reconstruction and selectively depicting the marrow reticulum cells could be obtained from thick glycol methacrylate sections reacted for Alkaline phosphatase. Furthermore, the yellow background of uncoupled diazonium salt over cytochemically unreactive structures in the same specimens and fields was used for imaging haemopoietic cell mass by operating the microscope at 488 nm (argon ion laser, blue-green). These methods may offer advantages in the investigation of the bone marrow stroma and its interplay with haemopoiesis and osteogenesis in normal and disease conditions.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13871366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nile red simultaneous staining of intracellular lipids and membrane network in human muscle cultures. 人肌肉细胞内脂质和膜网的尼罗红同步染色。
Basic and applied histochemistry Pub Date : 1989-01-01
I Santilli, A Prelle, L Geremia, G Scarlato, G Meola
{"title":"Nile red simultaneous staining of intracellular lipids and membrane network in human muscle cultures.","authors":"I Santilli,&nbsp;A Prelle,&nbsp;L Geremia,&nbsp;G Scarlato,&nbsp;G Meola","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have applied a new fluorescent probe, Nile red, on normal and pathological human muscle derived cultures and compared the results with corresponding human muscle sections. In normal human muscle cultures, Nile red strain has proved useful for visualization of both intracellular lipids and membrane network. Similar patterns have been observed in muscle cultures derived from lipid storage and mitochondrial myopathies. Moreover, abnormalities in pathological muscle cultures could be revealed by establishing more advanced culture systems.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13871368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Localization of actin in the wall of the subcapsular sinus of sheep haemal nodes. 肌动蛋白在绵羊淋巴结包膜下窦壁的定位。
Basic and applied histochemistry Pub Date : 1989-01-01
A M Gargiulo, V Pedini, P Ceccarelli
{"title":"Localization of actin in the wall of the subcapsular sinus of sheep haemal nodes.","authors":"A M Gargiulo,&nbsp;V Pedini,&nbsp;P Ceccarelli","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13871369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Densitometric evaluation of DNA contents in expanded human skin. 膨胀人体皮肤中DNA含量的密度测定。
Basic and applied histochemistry Pub Date : 1989-01-01
L Matturri, G L Campiglio, A M Lavezzi
{"title":"Densitometric evaluation of DNA contents in expanded human skin.","authors":"L Matturri,&nbsp;G L Campiglio,&nbsp;A M Lavezzi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We studied 18 patients who underwent skin expansion. In 3 patients the prostheses had been inserted in anatomical areas previously expanded: in 3 other patients scarring tissue had been involved in the expansion. The DNA content of sections of epidermis, before and after the implantation of expanders, were analysed using an image analyzer (IBAS 2000). The preliminary data demonstrate that, at the end of the expansion, epidermis enters a phase of quiescency and is not affected by dysplastic processes.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13770855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Digestion of chromatin DNA with DNAse I in situ: effect of ethanol fixation. 用DNA酶I原位消化染色质DNA:乙醇固定的影响。
Basic and applied histochemistry Pub Date : 1989-01-01
M G Bottone, E Ronchetti, C Pellicciari
{"title":"Digestion of chromatin DNA with DNAse I in situ: effect of ethanol fixation.","authors":"M G Bottone,&nbsp;E Ronchetti,&nbsp;C Pellicciari","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13770858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of fixation, exciting light and section thickness on the primary fluorescence of samples for microfluorometric analysis. 固定、激发光和切片厚度对微荧光分析样品初级荧光的影响。
Basic and applied histochemistry Pub Date : 1989-01-01
P Del Castillo, A R Llorente, J C Stockert
{"title":"Influence of fixation, exciting light and section thickness on the primary fluorescence of samples for microfluorometric analysis.","authors":"P Del Castillo,&nbsp;A R Llorente,&nbsp;J C Stockert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The primary fluorescence (autofluorescence) of some cell and tissue components depends on the fixative and fixing time, as well as on the thickness of paraffin sections and the wavelength of exciting light. The highest autofluorescence emission (pale green) was observed by using violet-blue excitation. After aldehyde fixation, the autofluorescence of some tissue structures was higher than after methanol or ethanolacetic acid. These features must be taken into account when fluorescence microscopy is applied to the study of cell smears and paraffin embedded tissues after flurochroming or immunofluorescence reactions.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13824349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A sexual dimorphism of the harderian gland of the toad, Bufo viridis. 蟾蜍硬腺的一种两性异形，绿蟾蜍
Basic and applied histochemistry Pub Date : 1989-01-01
S Minucci, G C Baccari, L Di Matteo, G Chieffi
{"title":"A sexual dimorphism of the harderian gland of the toad, Bufo viridis.","authors":"S Minucci,&nbsp;G C Baccari,&nbsp;L Di Matteo,&nbsp;G Chieffi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Harderian gland of the toad, Bufo viridis, is an acinar gland located at the medial corner of the orbit. The columnar glandular cells show considerable variation in height depending upon their functional state. During July they are taller than in November and May, and filled with secretory seromucous granules. The glandular cells of the female toad, only, contain at their base numerous lipid droplets dispersed in a smooth endoplasmic reticulum. This is the first observation of sexual dimorphism in the Harderian gland of a nonmammalian vertebrate. The secretion of the gland is mainly merocrine. Although the secretion of the Harderian gland is mainly concerned with lubrication of the eyeball, the presence of lipid secretion only in the female glandular cells suggests a pheromonale function, which may influence the sexual behaviour of the male.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13770854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A multifactorial study (cell kinetics, cytogenetics, DNA content) of lung tumours: basic information for prognostic evaluation. 肺肿瘤的多因素研究(细胞动力学，细胞遗传学，DNA含量):用于预后评估的基本信息。
Basic and applied histochemistry Pub Date : 1989-01-01
L Matturri, A M Lavezzi
{"title":"A multifactorial study (cell kinetics, cytogenetics, DNA content) of lung tumours: basic information for prognostic evaluation.","authors":"L Matturri,&nbsp;A M Lavezzi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Different prognostic parameters (labeling index, chromosomal pattern, DNA content) were studied in 32 cases of lung tumour. A great variability was observed in the proliferative activity and karyotype, and there was a lack of correlation between these parameters and the histologic type. It was possible to demonstrate that a diploid DNA content does not necessarily correspond to a normal karyotype. A more precise evaluation of the prognostic significance of data obtained will be possible in correlation with an extensive follow-up of the patients studied.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13770856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multi-organ damage (MOD) induced by cancer cachexia and its pathogenesis. 癌症恶病质诱导的多器官损伤及其发病机制。
Basic and applied histochemistry Pub Date : 1989-01-01
S Noriki, Y Imamura, T Ikeda, K Nakanishi, N Miyoshi, M Kohno, K Mitsuda, M Fukuda
{"title":"Multi-organ damage (MOD) induced by cancer cachexia and its pathogenesis.","authors":"S Noriki,&nbsp;Y Imamura,&nbsp;T Ikeda,&nbsp;K Nakanishi,&nbsp;N Miyoshi,&nbsp;M Kohno,&nbsp;K Mitsuda,&nbsp;M Fukuda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The intraperitoneal implantation of the ascitic hepatoma cells, AH-130 to rats induced marked atrophy of the systemic organs within 2 weeks, resulting in the animal death. By the method of Feulgen hydrolysis curve analysis, the amount of single-stranded DNA and the degree of DNA instability were shown to be increased in these atrophic organs. In keeping pace with the progression of the cachectic multi-organ damage (MOD), the amount of lipidperoxide and the activity of superoxide dismutase (SOD) as measured by electron spin resonance (ESR) were increased in the ascites toward the end stage of chachexia. The increased lipidperoxide and SOD induction reflect the increased production of active oxygens, especially superoxide. The marked systemic organ damage induced in cancer cachexia seems to be due to DNA damage by active oxygens.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13770857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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