Cytochemical evaluation of C-heterochromatic-DNA in metaphase chromosomes.

Basic and applied histochemistry Pub Date : 1990-01-01
C Pellicciari, E Ronchetti, R Tori, D Formenti, M G Manfredi Romanini
{"title":"Cytochemical evaluation of C-heterochromatic-DNA in metaphase chromosomes.","authors":"C Pellicciari,&nbsp;E Ronchetti,&nbsp;R Tori,&nbsp;D Formenti,&nbsp;M G Manfredi Romanini","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A method is proposed to evaluate the amount of DNA resistant to the C-banding pretreatments (C-heterochromatic-DNA) in metaphase chromosomes. Measurements were performed by microfluorometry on propidium iodide stained metaphases of man, gorilla and mouse; in these species, C-heterochromatin exhibits significant differences of both base composition and distribution along the chromosomes. The amount of C-heterochromatic-DNA was found to be about 16%, 28% and 58% of the total DNA content (genome size) in man, gorilla and mouse, respectively. The areas of C-bands after Giemsa staining were also assessed by microdensitometry, and corresponded to about 8%, 15% and 14% of the total karyotype area of man, gorilla and mouse respectively. No direct relation thus exists between C-band areas and the amount of DNA resistant to the C-banding pretreatments. In man and gorilla, the amount of C-heterochromatic-DNA accounts for the differences observed in genome size.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"34 1","pages":"79-85"},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Basic and applied histochemistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

A method is proposed to evaluate the amount of DNA resistant to the C-banding pretreatments (C-heterochromatic-DNA) in metaphase chromosomes. Measurements were performed by microfluorometry on propidium iodide stained metaphases of man, gorilla and mouse; in these species, C-heterochromatin exhibits significant differences of both base composition and distribution along the chromosomes. The amount of C-heterochromatic-DNA was found to be about 16%, 28% and 58% of the total DNA content (genome size) in man, gorilla and mouse, respectively. The areas of C-bands after Giemsa staining were also assessed by microdensitometry, and corresponded to about 8%, 15% and 14% of the total karyotype area of man, gorilla and mouse respectively. No direct relation thus exists between C-band areas and the amount of DNA resistant to the C-banding pretreatments. In man and gorilla, the amount of C-heterochromatic-DNA accounts for the differences observed in genome size.

中期染色体c-异色dna的细胞化学评价。
提出了一种评估中期染色体中抗c带预处理的DNA数量(c-异色DNA)的方法。用微量荧光法测定人、大猩猩和小鼠碘化丙啶染色中期细胞;在这些物种中,c -异染色质在碱基组成和沿染色体的分布上都有显著差异。在人类、大猩猩和小鼠中,c-异色DNA的含量分别约占总DNA含量(基因组大小)的16%、28%和58%。用微密度法测定吉氏染色后的c带面积,分别占人、大猩猩和小鼠核型总面积的8%、15%和14%左右。因此,c带面积与DNA对c带预处理的抗性数量之间不存在直接关系。在人类和大猩猩中,c-异色dna的数量解释了在基因组大小上观察到的差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信