超微结构免疫细胞化学方法鉴定软骨鱼胰高血糖素细胞。

Basic and applied histochemistry Pub Date : 1990-01-01
G Faraldi, M Canepa, L Borgiani, T Zanin
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引用次数: 0

摘要

研究了胰高血糖素的超微结构定位。我们采用包埋后免疫电镜法对胰腺样品进行戊二醛固定和包埋前渗滤处理。免疫标记后与醋酸铀酰和柠檬酸铅进行对比,以醋酸铀酰为免疫标记剂效果最好。胰高血糖素样免疫反应性位于被限制膜包围的圆形颗粒(300-600 nm)内。基体的电子密度变化很大,呈现出致密的核被密度较小的地幔包围。颗粒在两种不同的细胞类型中可见,其细胞质的电子密度不同。胰高血糖素免疫反应细胞是最大的胰腺细胞类型,通常定位于含有生长抑素的细胞附近。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of pancreatic glucagon cells in the cartilaginous fish Scyliorhinus canicula by ultrastructural immunocytochemistry.

The ultrastructural localization of glucagon in the presence of Scyliorhinus canicula was investigated. We used a post-embedding immunoelectron microscopy method on pancreatic samples fixed in glutaraldehyde and osmicated before embedding. Contrasting with uranyl acetate and lead citrate was also performed after immunolabelling, but best results were obtained with uranyl acetate only. Glucagon-like immunoreactivity was located in round granules (300-600 nm) surrounded by a limiting membrane. The matrix varied in electron density and exhibited a dense core surrounded by a less dense mantle. The granules were seen in two different cell types, which differed in the electron density of their cytoplasm. Glucagon-immunoreactive cells were the largest pancreatic cells types and were often localized near somatostatin-containing cells.

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