中期染色体c-异色dna的细胞化学评价。

Basic and applied histochemistry Pub Date : 1990-01-01
C Pellicciari, E Ronchetti, R Tori, D Formenti, M G Manfredi Romanini
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引用次数: 0

摘要

提出了一种评估中期染色体中抗c带预处理的DNA数量(c-异色DNA)的方法。用微量荧光法测定人、大猩猩和小鼠碘化丙啶染色中期细胞;在这些物种中,c -异染色质在碱基组成和沿染色体的分布上都有显著差异。在人类、大猩猩和小鼠中,c-异色DNA的含量分别约占总DNA含量(基因组大小)的16%、28%和58%。用微密度法测定吉氏染色后的c带面积,分别占人、大猩猩和小鼠核型总面积的8%、15%和14%左右。因此,c带面积与DNA对c带预处理的抗性数量之间不存在直接关系。在人类和大猩猩中,c-异色dna的数量解释了在基因组大小上观察到的差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytochemical evaluation of C-heterochromatic-DNA in metaphase chromosomes.

A method is proposed to evaluate the amount of DNA resistant to the C-banding pretreatments (C-heterochromatic-DNA) in metaphase chromosomes. Measurements were performed by microfluorometry on propidium iodide stained metaphases of man, gorilla and mouse; in these species, C-heterochromatin exhibits significant differences of both base composition and distribution along the chromosomes. The amount of C-heterochromatic-DNA was found to be about 16%, 28% and 58% of the total DNA content (genome size) in man, gorilla and mouse, respectively. The areas of C-bands after Giemsa staining were also assessed by microdensitometry, and corresponded to about 8%, 15% and 14% of the total karyotype area of man, gorilla and mouse respectively. No direct relation thus exists between C-band areas and the amount of DNA resistant to the C-banding pretreatments. In man and gorilla, the amount of C-heterochromatic-DNA accounts for the differences observed in genome size.

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