Hinf I restriction endonuclease digestion on human fixed metaphase chromosomes.

Basic and applied histochemistry Pub Date : 1990-01-01
L Stuppia, P Guanciali Franchi, G Calabrese, G Parruti, G Palka, U Bianchi
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Abstract

The authors report on the activity of Hinf I restriction endonuclease on human fixed metaphase chromosomes. Experiments performed by digesting chromosomes just after harvesting or after ageing in methanol-acetic acid displayed a different pattern of digestion on metaphases, since only aged preparations showed gaps on heterochromatic regions of chromosomes 1, 9 and 16 and C-like bands on other chromosomes. In this view, the authors suggest that structural modifications of the DNA, induced by acid fixation, can influence Hinf I activity on fixed metaphase chromosomes.

人类固定中期染色体上的hinif限制性内切酶酶切。
作者报道了人类固定中期染色体上hinfi限制性内切酶的活性。在收获后和老化后的甲醇乙酸中进行的消化实验显示,只有老化后的样品在1、9和16号染色体的异色区和其他染色体上的c样带上出现了间隙,因此在中期的消化模式不同。在这种观点下,作者认为酸固定诱导的DNA结构修饰可以影响固定中期染色体上的Hinf I活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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