不同阿利西亚蓝蛋白多糖颗粒在椎间盘的定位。

Basic and applied histochemistry Pub Date : 1988-01-01
F Ortolani, M Raspanti, M Franchi, M Marchini
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引用次数: 0

摘要

对牛椎间盘蛋白多糖进行了组织化学研究。从“纤维环”(A.F.)和“髓核”(N.P.)中获得的样品用阿利新蓝(AB)在临界电解质浓度(CEC)的MgCl2溶液中稀释处理;部分标本在AB治疗前在睾丸透明质酸酶中孵育。至少有四种类型的ab -蛋白多糖颗粒被识别出来:a)在A.F.片层和n.p.中,1 nm的棒状颗粒与胶原原纤维垂直排列,间距相当于原纤维的d周期(图1-5);b)在A.F.薄片内,其他16-20 nm的颗粒在胶原原纤维之间形成紧密的网络(图1、2、3、5);c) A.F.层间裂隙中存在30 ~ 50 nm叶状颗粒(图6);d)在N.P.Z中,20-30 nm的叶状颗粒形成了一个宽网状结构(图4)。alcianophyllic颗粒大小表明它们可能对应于A.F.片层中的蛋白多糖单体,并且大部分蛋白多糖聚集在A.F.片层间隙和N.P中。MgCl2 CEC溶液中的嗜酸度和酶敏感性都表明存在硫酸软骨素和硫酸角蛋白,并且A.F.层间缝隙中的大颗粒是硫酸角蛋白最丰富的蛋白聚糖。观察到的ab蛋白聚糖颗粒的特征与先前报道的其他组织的形态学数据以及椎间盘的一些生化数据一致,并可能与该组织的复合力学性能有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Localization of different alcian blue-proteoglycan particles in the intervertebral disc.

A histochemical investigation was carried out on proteoglycans of bovine intervertebral disc. Samples obtained from the "annulus fibrosus" (A.F.) and "nucleus pulposus" (N.P.) were treated with Alcian blue (AB) diluted in solutions of MgCl2 at critical electrolyte concentrations (CEC); some samples were incubated in testicular hyaluronidase before AB treatment. At least four types of elongated AB-proteoglycan particles were recognized: a) in A.F. lamellae and N.P., 1 nm rod-like particles were arranged orthogonally to the collagen fibrils and spaced at a distance equivalent to the fibril D-period (Figs. 1-5); b) within the A.F. lamellae, other 16-20 nm particles formed a close network among the collagen fibrils (Figs. 1,2,3,5); c) in the A.F. interlamellar crevices, 30-50 nm leaf-like particles were present (Fig. 6); d) in the N.P.Z., 20-30 nm leaf-like particles formed a wide-mesh (Fig. 4). The alcianophylic particle sizes suggest they may correspond to proteoglycan monomers in the A.F. lamellae and mostly proteoglycan aggregates in A.F. interlamellar crevices and N.P.. Both alcianophylia degrees at MgCl2 CEC solutions and enzymatic susceptibility indicate the presence of chondroitin sulphate and keratan sulphate and that the large particles in the A.F. interlamellar crevices are the keratan sulphate richest proteoglycans. The features of the observed AB-proteoglycan particles are consistent with previous morphological data reported for other tissues as well as some biochemical data for the intervertebral disc and may be correlated to the composite mechanical properties of this tissue.

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