成年家鸡髓质和中脑神经元群对VIP、生长抑素和bombesin抗体的反应。

Basic and applied histochemistry Pub Date : 1988-01-01
G Nicolardi, G Ambrosi, A Rizzi, M E Camosso
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引用次数: 0

摘要

采用PAP-DAB方法对成年鸡髓背区尾-颅中间三分之一、导水管周围灰质和中脑被盖的前-腹侧部分进行了详细的研究,以进一步确定在先前的研究中显示VIP(血管活性肠多肽)、-生长抑素(SRIF)-和bombesin样免疫反应的神经元群的主要形态学特征。在髓质中,在神经元体和突起内检测到vip样免疫反应,并向下延伸到颈脊髓。srif样免疫反应性仅在神经细胞突起中可见,其中至少一部分可能是敏感的纤维末梢。仅在神经元过程中观察到炸弹素样免疫反应性。在导水管周围灰色,核周和神经元突内检测到所有3种免疫反应,颅骨密度较高。在中脑被盖的前腹侧部分,在神经元体和突起中都检测到vip样和bombesin样免疫反应(后者在更大程度上位于颅骨)。srif样免疫反应仅在长神经元突起中见于该区域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Medullary and mesencephalic neuronal groups reacting to antibodies against VIP, somatostatin and bombesin in adult Gallus gallus domesticus.

The caudo-cranially intermediate one-third of medullary dorsal region, the periaqueductal grey and the rostro-ventral portion of the midbrain tegmentum of adult chickens were studied in detail by means of the PAP-DAB procedure, to define further the main morphological features of the neuronal populations that in previous studies had shown VIP (Vasoactive Intestinal Polypeptide),-Somatostatin (SRIF)-, and Bombesin-like immunoreactivities. In the medulla, VIP-like immunoreactivity was detected within neuronal bodies and processes and extended down to the cervical spinal cord. SRIF-like immunoreactivity was seen only within nerve cell processes, at least a part of which could be sensitive fibre terminals. Bombesin-like immunoreactivity was observed only within neuronal processes. In the periaqueductal grey, all 3 immunoreactivities were detected within perikarya and neuronal processes, with a higher density cranially. In the rostro-ventral portion of the midbrain tegmentum, VIP-like and Bombesin-like immunoreactivities were detected (the latter being located somewhat more cranially) both in neuronal bodies and in processes. SRIF-like immunoreactivity was found in this region only in long neuronal processes.

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