凝集素组织化学对不同细胞类型糖结合物的检测和分化。

Basic and applied histochemistry Pub Date : 1988-01-01
S S Spicer, B A Schulte
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引用次数: 0

摘要

通过使用一组辣根过氧化物酶结合凝集素的组织化学方法,我们对精确位点上的糖缀合物(gc)的化学结构有了更多的了解,这些凝集素对特定的末端糖或内部糖链具有亲和力。凝集素组织化学在不同组织部位的GCs中显示出巨大的差异性,反映了这些物质已知的化学多样性。在动物体内不同类型的细胞中,GCs的结构不同,在特定的组织学位置上,物种之间或近亲繁殖而非近亲繁殖的物种之间的GCs存在差异。凝集素偶联物与GCs发生反应并检测到组织化学上无法证明的GCs,并且由于其在组织中的浓度较低,因此无法进行生化鉴定。凝集素- hrp偶联物在大鼠胚胎的原始生殖细胞中具有独特的GalNAc末端GC,在啮齿动物的上皮基底细胞和大鼠的Ranvier淋巴结中具有GalNAc末端,在某些小鼠的胸腺、Peyer’s斑块和肠固有层的细胞群中具有GalNAc末端。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection and differentiation of glycoconjugates in various cell types by lectin histochemistry.

Knowledge of chemical structure of glycoconjugates (GCs) at precise loci has increased through histochemical use of a battery of horseradish peroxidase-conjugated lectins, each possessing affinity for a specific terminal sugar or internal sugar linkage. Lectin histochemistry has shown tremendous variability among GCs in different histologic sites, reflecting known chemical diversity of these substances. GCs differ in structure among various cell types in an animal and differ at a given histologic site between species or between individuals in outbred but not inbred species. Lectin conjugates react with and detect GCs not otherwise demonstrable histochemically and, because of low concentration in tissue, not identified biochemically. Lectin-HRP conjugates have visualized unique GC with terminal GalNAc in primordial germ cells of rat embryos, with terminal Gal in epithelial basal cells of rodents and nodes of Ranvier in rats and with terminal GalNAc in a cell population in the thymus, Peyer's patches and intestinal lamina propria of some but not other mice.

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