Artificial cells, blood substitutes, and immobilization biotechnology最新文献_第4页

Sulfite determination by an inhibitor biosensor-based mushroom (Agaricus bisporus) tissue homogenate. 基于生物传感器的蘑菇(Agaricus bisporus)组织匀浆抑制剂测定亚硫酸盐。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-06-30 DOI: 10.3109/10731199.2011.585614

Mustafa Kemal Sezgintürk, Erhan Dinçkaya

引用次数: 2

Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant. 多血红蛋白-超氧化物歧化酶-过氧化氢酶-碳酸酐酶:一种新型的基于生物技术的血液替代品，可运输氧气和二氧化碳，也可作为抗氧化剂。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-06-21 DOI: 10.3109/10731199.2011.582041

Yuzhu Bian, Zhixia Rong, Thomas Ming Swi Chang

引用次数: 4

Packing density of the PEG-shell in PEG-albumins: PEGylation induced viscosity and COP are inverse correlate of packing density. 聚乙二醇白蛋白中聚乙二醇壳的堆积密度:聚乙二醇化引起的黏度和COP与堆积密度成反比。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-05-31 DOI: 10.3109/10731199.2011.579568

K Ananda, Belur N Manjula, Fantao Meng, Vivek N Acharya, Marcos Intaglietta, Seetharama A Acharya

{"title":"Packing density of the PEG-shell in PEG-albumins: PEGylation induced viscosity and COP are inverse correlate of packing density.","authors":"K Ananda, Belur N Manjula, Fantao Meng, Vivek N Acharya, Marcos Intaglietta, Seetharama A Acharya","doi":"10.3109/10731199.2011.579568","DOIUrl":"https://doi.org/10.3109/10731199.2011.579568","url":null,"abstract":"PEG-Alb represents a new class of low viscogenic plasma expanders that achieve super perfusion in vivo by mimicking the vasodilatory influence of high viscogenic plasma expanders. PEGylation-engineered structure of PEG albumin can be envisaged as a deformable molecular domain around the rigid central protein core. The correlation between the structure of PEG-shell in terms of packing of the PEG inside the PEG shell and PEGylation induced plasma expander (PE)-like properties of albumin has been investigated as a function of the number and length of the PEG-chain. The increase in molecular radius of albumin on PEGylation is non-linear as a function of the number of PEG chains conjugated. The packing density of PEG within the PEG-shell is an inverse correlate of PEG-chain size; i.e. the shorter chains pack more compactly than the longer ones. The PEGylation induced increase in the viscosity and COP of albumin is an exponential correlation of the number of ethylene oxide units (-CH(2)-CH(2)-O-) conjugated and is also a function of the PEG-chain length. At equivalence of PEG mass conjugated, the viscosity and COP of PEG-albumin adducts correlate inversely with packing density of PEG. All PEGylated albumins are not equivalent on the basis of total PEG mass conjugated. Accordingly, the structure of PEG albumin and its solution properties can be engineered to optimize a given total PEG mass for the application of PEG albumin as a resuscitation fluid. The extension arms minimize the influence of PEG shell on the structure of the protein core. We speculate that EAF-PEGylation is a preferable platform for PEGylation of protein therapeutics and is expected to generate products with better therapeutic efficacy.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.579568","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29899460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Polymerized human placenta hemoglobin (PolyPHb) attenuates myocardial infarction injury in rats. 聚合人胎盘血红蛋白(PolyPHb)减轻大鼠心肌梗死损伤。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-05-31 DOI: 10.3109/10731199.2011.579567

Yuxin Wang, Xiangfang Zhao, Fengjuan Li, Weiping Chen, Lei Wang, Chengmin Yang

{"title":"Polymerized human placenta hemoglobin (PolyPHb) attenuates myocardial infarction injury in rats.","authors":"Yuxin Wang, Xiangfang Zhao, Fengjuan Li, Weiping Chen, Lei Wang, Chengmin Yang","doi":"10.3109/10731199.2011.579567","DOIUrl":"https://doi.org/10.3109/10731199.2011.579567","url":null,"abstract":"Objectives: To investigate the cardioprotective effect of polymerized human placenta hemoglobin (PolyPHb) for acute myocardial ischemia rat heart.Methods: Myocardial infarcts (MI) model was set up in SD rats by permanent ligation of the left anterior descending coronary artery (LDA). The rats were divided randomly into 3 groups with each group of 20 rats: (A) the control group with the administration of Ringer's lactated solution at a dose of 2.5ml/kg); (B) PolyPHb group, PolyPHb solution at a dose of 0.16g Hb/kg and (C), PolyPHb+CAT+SOD group, PolyPHb solution at a dose of 0.16g Hb/kg and catalase (CAT) solution and superoxide dismutase (SOD) solution at a dose of 1681 U/kg and 528000 U/kg, respectively. Each rat received treatments via caudal vein, once a day for 7 days. Qualitative evaluations were made based on the reading of cardiac troponin T (cTnT), the myocardial infarction size (MIS) derived from the staining of myocardium tissue, and the pathological changes in infarct area. The ischemia changes of cardiomyocytes were determined by haematoxylin - basic fuchsin - picric acid (HBFP) staining and the apoptosis of cardiomyocytes were evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay (TUNEL).Results: Compared to the control group, PolyPHb greatly decreased the cTnT (p < 0.05), MIS (p < 0.05), and the size of myocardial ischemia (p < 0.05). PolyPHb + CAT + SOD decreased the △ST change (P < 0.05), cTnT (P < 0.01), MIS (p < 0.05), the pathological scores (p < 0.01), the size of myocardial ischemia (p < 0.01), and the apoptosis level (p < 0.01).Conclusion: Our study demonstrated that adding PolyPHb improves cardiac functional recovery and reduces myocardial infarction of rat heart.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.579567","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29899461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Microencapsulated Saccharomyces cerevisiae column bioreactor for potential use in renal failure uremia. 微胶囊化酿酒酵母柱生物反应器在肾衰竭尿毒症中的潜在应用。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 DOI: 10.3109/10731199.2011.597758

Razek Georges Coussa, Sapna Shah, Poonam Jain, Christopher Martoni, Jasmine Bhathena, Meenakshi Malhotra, Satya Prakash

引用次数: 1

PK11195, a specific ligand of the peripheral benzodiazepine receptor, may protect pancreatic beta-cells from cytokine-induced cell death. PK11195是外周苯二氮卓受体的特异性配体，可能保护胰腺β细胞免受细胞因子诱导的细胞死亡。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-08-02 DOI: 10.3109/10731199.2011.585617

Roland A Klaffschenkel, Marc Waidmann, Hinnak Northoff, Amr A A Mahmoud, Nicolas Lembert

引用次数: 2

α-Amylase monitoring by a novel amperometric biosensor based on Au electrode: its optimization, characterization, and application. 基于Au电极的新型安培生物传感器α-淀粉酶监测:优化、表征及应用

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-08-12 DOI: 10.3109/10731199.2011.597757

Umut Mengulluoglu, Cagri Altug, H Deniz Ertugrul, Abdulkerim Yildiz, E Melis Ekici, Erhan Dinckaya

引用次数: 5

Preparation and influencing factors of sirolimus liposome by supercritical fluid. 超临界流体法制备西罗莫司脂质体及其影响因素。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-06-21 DOI: 10.3109/10731199.2011.585618

Wei Zhang, Yong Sun, Yongjian Li, Ruowu Shen, Hongli Ni, Dejian Hu

引用次数: 23

Fluid resuscitation of hemorrhagic shock with hemoglobin vesicles in Beagle dogs: pilot study. Beagle犬失血性休克伴血红蛋白囊泡液体复苏的初步研究。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 DOI: 10.3109/10731199.2011.637929

Manabu Yamamoto, Hirohisa Horinouchi, Koichi Kobayashi, Yasuhisa Seishi, Natsue Sato, Manabu Itoh, Hiromi Sakai

{"title":"Fluid resuscitation of hemorrhagic shock with hemoglobin vesicles in Beagle dogs: pilot study.","authors":"Manabu Yamamoto, Hirohisa Horinouchi, Koichi Kobayashi, Yasuhisa Seishi, Natsue Sato, Manabu Itoh, Hiromi Sakai","doi":"10.3109/10731199.2011.637929","DOIUrl":"https://doi.org/10.3109/10731199.2011.637929","url":null,"abstract":"Resuscitation of hemorrhagic shock requires volume replacement and restoration of oxygen metabolism. Artificial oxygen carriers that can both expand blood volume and deliver oxygen have been developed as resuscitation fluids. We employed hemoglobin vesicles (HbV), a cellular-type artificial oxygen carrier, in a Beagle dog hemorrhagic shock model to prove the efficacy of HbV. Hemorrhagic shock was introduced in splenectomized Beagle dogs by withdrawing 50% of circulating blood from the femoral artery. Shock was maintained for 60 minutes before isovolemic resuscitation with HbV dispersed in 5% albumin in saline (HbV), lactated Ringer's solution (LR), 5% human serum albumin in saline (HSA), or autologous shed blood (ASB). One animal in the LR group died 150 min after resuscitation. All other animals survived 4 h of the experiment. The mean arterial pressure remained significantly lower in the LR group than in the HbV group but did not differ significantly among the HbV, Alb, and ASB groups. Immediately after resuscitation, the HbV group showed a significantly higher mean pulmonary arterial pressure, which decreased within 10 minutes to the baseline level. The cardiac output was significantly higher in the Alb group than in the others, indicating compensation for low oxygen delivery per unit blood. The post-resuscitation hematocrit was 36% in the ASB group and decreased in the other groups (20-22%). Serum chemistry data from the HbV group were unremarkable. HbV contributed 32% of the post-resuscitation oxygen delivery. Collectively, HbV is comparable to ASB and HSA as a resuscitation fluid and is an effective oxygen carrier.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.637929","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30422241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Microencapsulated mammalian cells for simultaneous production of VEGF165b and IFNα. 微囊化哺乳动物细胞同时产生VEGF165b和IFNα。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 DOI: 10.3109/10731199.2011.560120

Fatemeh Afkhami, Yves Durocher, Satya Prakash

引用次数: 0