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Artificial cells, blood substitutes, and immobilization biotechnology最新文献

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Comparison of covalent and noncovalent immobilization of Malatya apricot pectinesterase (Prunus armeniaca L.). 马来杏果胶酯酶共价与非共价固定化的比较。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-09-27 DOI: 10.3109/10731199.2011.611471
Emine Karakuş, Sule Pekyardımcı
{"title":"Comparison of covalent and noncovalent immobilization of Malatya apricot pectinesterase (Prunus armeniaca L.).","authors":"Emine Karakuş,&nbsp;Sule Pekyardımcı","doi":"10.3109/10731199.2011.611471","DOIUrl":"https://doi.org/10.3109/10731199.2011.611471","url":null,"abstract":"<p><p>Pectinesterase isolated from Malatya apricot pulp was noncovalently and covalently immobilized onto bentonite and glutaraldehyde-containing amino group functionalized porous glass beads surface at pH 8.0 and pH 9.0, respectively. The effect of various parameters such as pH, temperature, activation energy, heat and storage stability on immobilized enzyme were investigated. The optimum temperature of covalently and noncovalently immobilized PE was 50°C. This value was 60°C for free PE. Although optimum pH of covalently-immobilized PE was 8.0, this parameter was 9.0 for free and covalently-immobilized PE. The noncovalently immobilized enzyme exhibited better thermostability than the free and covalently immobilized PE.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.611471","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30168989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Decellularization of heart valve matrices: search for the ideal balance. 心脏瓣膜基质的脱细胞:寻找理想的平衡。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-12-22 DOI: 10.3109/10731199.2011.637925
Pamela Somers, Filip De Somer, Maria Cornelissen, Hubert Thierens, Guido Van Nooten
{"title":"Decellularization of heart valve matrices: search for the ideal balance.","authors":"Pamela Somers,&nbsp;Filip De Somer,&nbsp;Maria Cornelissen,&nbsp;Hubert Thierens,&nbsp;Guido Van Nooten","doi":"10.3109/10731199.2011.637925","DOIUrl":"https://doi.org/10.3109/10731199.2011.637925","url":null,"abstract":"<p><strong>Objective: </strong>Currently used decellularization procedures have negative effects on extracellular matrix (ECM) integrity. The objective of this study is to evaluate four decellularization methods and their effect on the collagen ultrastructure, mechanical behavior and antigenicity of porcine aortic valves.</p><p><strong>Methods: </strong>Aortic valves were placed in a trypsin, osmotic, trypsin-osmotic or detergent-osmotic solution. Leaflets were processed for histology and mechanical testing. Matrices were implanted subdermally in rats to evaluate immune reaction and calcification.</p><p><strong>Results: </strong>Trypsin-osmotic methodology effected near-complete decellularization. Trypsin treatment resulted in cell removal only in the spongiosa layer. Osmotic and detergent-osmotic treatments did not remove any cells from the cusps. Mechanical strength was significantly inferior in the trypsin (p50,03) and trypsin-osmotic treated group (p50,04). Trypsin and trypsin-osmotic decellularized matrices evoked a strong CD31 inflammatory cell infiltration.</p><p><strong>Conclusion: </strong>Enzymatic-osmotic decellularization appears to be the only effective method to remove cellular components. However, the near cell free scaffolds still evokes a strong CD31 T-cell inflammatory reaction.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.637925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30343874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 34
Cellular biocompatibility and biomechanical properties of N-carboxyethylchitosan/nanohydroxyapatite composites for tissue-engineered trachea. 组织工程气管用n -羧乙基壳聚糖/纳米羟基磷灰石复合材料的细胞生物相容性和生物力学性能。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-08-02 DOI: 10.3109/10731199.2011.597760
Hongcan Shi, Wanpeng Wang, Dan Lu, Haijia Li, Linsong Chen, Yan Lu, Yanjun Zeng
{"title":"Cellular biocompatibility and biomechanical properties of N-carboxyethylchitosan/nanohydroxyapatite composites for tissue-engineered trachea.","authors":"Hongcan Shi,&nbsp;Wanpeng Wang,&nbsp;Dan Lu,&nbsp;Haijia Li,&nbsp;Linsong Chen,&nbsp;Yan Lu,&nbsp;Yanjun Zeng","doi":"10.3109/10731199.2011.597760","DOIUrl":"https://doi.org/10.3109/10731199.2011.597760","url":null,"abstract":"<p><p>To prepare an NCECS/nHA composite for tissue-engineered trachea and investigate its biomechanical and biocompatibile properties. Biomechanical tests were performed on dry and wet NCECS/nHA composite specimens prepared in vitro. The cell adhesion rate on each composite surface after 2, 6, and 12 hours of culture was calculated, and cell proliferation activity was measured using an MTT assay. NCECS/nHA composites exhibited satisfactory tensile strength and Young's modulus values. The adhesion rate of rabbit tracheal chondrocytes on NCECS/nHA surfaces reached 88.4% after 12 hours of culture. NCECS/nHA composites are promising scaffold materials for tissue-engineered trachea owing to satisfactory biocompatible and biomechanical properties.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.597760","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29904330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Pre-hospital resuscitation with HBOC-201 and rFVIIa compared to HBOC-201 alone in uncontrolled hemorrhagic shock in swine. HBOC-201和rFVIIa在猪失控失血性休克中的院前复苏比较
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-08-02 DOI: 10.3109/10731199.2011.585615
Ashraful Haque, Françoise Arnaud, Kohsuke Teranishi, Tomoaki Okada, Bobby Kim, Paula F Moon-Massat, Charles Auker, Richard McCarron, Daniel Freilich, Anke H Scultetus
{"title":"Pre-hospital resuscitation with HBOC-201 and rFVIIa compared to HBOC-201 alone in uncontrolled hemorrhagic shock in swine.","authors":"Ashraful Haque,&nbsp;Françoise Arnaud,&nbsp;Kohsuke Teranishi,&nbsp;Tomoaki Okada,&nbsp;Bobby Kim,&nbsp;Paula F Moon-Massat,&nbsp;Charles Auker,&nbsp;Richard McCarron,&nbsp;Daniel Freilich,&nbsp;Anke H Scultetus","doi":"10.3109/10731199.2011.585615","DOIUrl":"https://doi.org/10.3109/10731199.2011.585615","url":null,"abstract":"<p><p>In a previous dose escalation study our group found that combining 90μg/kg rFVIIa with HBOC-201 reduced blood loss and improved physiologic parameters compared to HBOC alone. In this follow-up study in a swine liver injury model, we found that while there were no adverse hematology effects and trends observed in the previous study were confirmed, statistical significance could not be reached. Additional pre-clinical studies are indicated to identify optimal components of a multifunctional blood substitute for clinical use in trauma.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.585615","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29904333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Development of an amperometric polyphenol biosensor based on fungal laccase immobilized on nitrocellulose membrane. 硝化纤维素膜固定化真菌漆酶安培型多酚生物传感器的研制。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-12-22 DOI: 10.3109/10731199.2011.637926
Chandra Shekhar Pundir, Rachna Rawal, Sheetal Chawla, Renuka, Ramesh Chandra Kuhad
{"title":"Development of an amperometric polyphenol biosensor based on fungal laccase immobilized on nitrocellulose membrane.","authors":"Chandra Shekhar Pundir,&nbsp;Rachna Rawal,&nbsp;Sheetal Chawla,&nbsp;Renuka,&nbsp;Ramesh Chandra Kuhad","doi":"10.3109/10731199.2011.637926","DOIUrl":"https://doi.org/10.3109/10731199.2011.637926","url":null,"abstract":"<p><p>A method is described for construction of an amperometric polyphenol biosensor employing nitrocellulose membrane-bound laccase purified from cell-free extract of Ganoderma lucidum onto a Pt electrode. The biosensor showed optimum response within 10s, at 0.4 V in 0.1M acetate buffer, pH 6.0, and 35°C. Detection limit of the biosensor was 3.0 × 10(-8)M. Analytical recovery of added guaiacol was 97.00%. Within batch and between batch coefficients of variation were <0.97% and <1.26%, respectively. The sensor measured total phenolic content in fruit juices and alcoholic beverages. The enzyme electrode was used 100 times over 4 months, when stored at 4°C.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.637926","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30343875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Advances in pulmonary delivery of nanoparticles. 纳米颗粒肺递送的研究进展。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-08-02 DOI: 10.3109/10731199.2011.592494
Gunjeet Kaur, R K Narang, Goutam Rath, Amit K Goyal
{"title":"Advances in pulmonary delivery of nanoparticles.","authors":"Gunjeet Kaur,&nbsp;R K Narang,&nbsp;Goutam Rath,&nbsp;Amit K Goyal","doi":"10.3109/10731199.2011.592494","DOIUrl":"https://doi.org/10.3109/10731199.2011.592494","url":null,"abstract":"<p><p>Nanotechnology has potential in the development of novel and effective delivery of drugs within lungs. Different strategies have been utilized for pulmonary delivery of drugs, including the use of lipid-based delivery systems (liposomes, ISCOMs, SLNs), use of polymeric matrix (PLGA, poly caprolactone, cynoacrylates, gelatin), development of polysaccharide particulates (chitosan, alginates, Carbopol, etc.), biocompatible metallic inorganic particles (iron, gold, zinc), etc. This paper reviews various nanopaticulate approaches in the form of lipids, polymers, metals, polysaccharides, or emulsions based for pulmonary drug delivery that could provide an increased biological efficacy and better local and systemic action.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.592494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29904331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 49
Nanoparticles based on hydrophobic alginate derivative as nutraceutical delivery vehicle: vitamin D3 loading. 基于疏水藻酸盐衍生物的纳米颗粒作为营养保健品递送载体:维生素D3装载。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-08-02 DOI: 10.3109/10731199.2011.597759
Fusheng Sun, Chuanxia Ju, Jiahong Chen, Sai Liu, Na Liu, Keke Wang, Chenguang Liu
{"title":"Nanoparticles based on hydrophobic alginate derivative as nutraceutical delivery vehicle: vitamin D3 loading.","authors":"Fusheng Sun,&nbsp;Chuanxia Ju,&nbsp;Jiahong Chen,&nbsp;Sai Liu,&nbsp;Na Liu,&nbsp;Keke Wang,&nbsp;Chenguang Liu","doi":"10.3109/10731199.2011.597759","DOIUrl":"https://doi.org/10.3109/10731199.2011.597759","url":null,"abstract":"<p><p>We report the application of Vitamin D3 (VD(3)) in nanoparticles of oleoyl alginate ester (OAE)(OAE-VD(3)). The internalization of fluorescent OAE-VD(3) by Caco-2 cells was visualized by confocal laser scanning microscopy. In vivo pharmacokinetic studies showed that incorporation into OAE nanoparticles resulted in increased absorption of VD(3). Its application in the treatment of rickets was assayed using a model of nutritionally induced vitamin D-deficiency rickets. The results showed that the encapsulated VD(3) had better efficacy than that of the free drug in vivo. Our studies provide evidence that OAE nanoparticles are valuable as nutraceutical delivery vehicles to enhance the absorption of VD(3).</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.597759","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29904334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
Gene expression profile of the synovium and cartilage in a chronic arthritis rat model. 慢性关节炎模型大鼠滑膜和软骨的基因表达谱。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-07-15 DOI: 10.3109/10731199.2011.592493
Weimin Zhu, Daping Wang, Wei Lu, Yun Han, Yangkan Ou, Ke Zhou, Liangquan Peng, Wenzhe Feng, Hao Li, Qi Chen, Kai Zhang, Yanjun Zeng, Xiaojun Zhang
{"title":"Gene expression profile of the synovium and cartilage in a chronic arthritis rat model.","authors":"Weimin Zhu,&nbsp;Daping Wang,&nbsp;Wei Lu,&nbsp;Yun Han,&nbsp;Yangkan Ou,&nbsp;Ke Zhou,&nbsp;Liangquan Peng,&nbsp;Wenzhe Feng,&nbsp;Hao Li,&nbsp;Qi Chen,&nbsp;Kai Zhang,&nbsp;Yanjun Zeng,&nbsp;Xiaojun Zhang","doi":"10.3109/10731199.2011.592493","DOIUrl":"https://doi.org/10.3109/10731199.2011.592493","url":null,"abstract":"<p><strong>Background: </strong>Primary osteoarthritis (OA) is a polygenic disease. To investigate the gene expression profile of cartilage and synovium from osteoarthritis and healthy rats using cDNA microarray is beneficial to recognize the pathogenesis of osteoarthritis and provide evidence for gene therapy of osteoarthritis.</p><p><strong>Objective: </strong>The present study aimed to investigate the gene expression profile of the cartilage and synovium of chronic arthritis and healthy rats through cDNA microarray assay, and identify the differentially expressed genes. This study may be helpful for understanding the role of differentially expressed genes in osteoarthritis and the gene polymorphism of osteoarthritis.</p><p><strong>Methods: </strong>A total of 24 male Wistar rats were randomly divided into control group and osteoarthritis group (n = 12 per group). The synovial and cartilage were obtained and total RNA was extracted. cDNA microarray assay was performed to identify the differentially expressed genes, and cluster analysis was conducted.</p><p><strong>Results and conclusion: </strong>A total of 82 differentially expressed genes were identified, among which 27 were up-regulated and 55 down-regulated. Gene microarray assay is effective to identify differentially expressed genes and may find out novel osteoarthritis associated genes. Multiple genes are involved in the pathogenesis of osteoarthritis. The differentially expressed genes provide important information for further studies on the pathogenesis of osteoarthritis and gene therapy of osteoarthritis.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.592493","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30006192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Temperature effects on enzyme activity of chicken liver esterase used in calorimetric biosensor. 温度对量热生物传感器用鸡肝酯酶活性的影响。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-09-26 DOI: 10.3109/10731199.2011.611470
Yi-Hua Zheng, Jun Liu, Yong-Zhi Ma, Yi Xu, Fei Xu, Tse-Chao Hua
{"title":"Temperature effects on enzyme activity of chicken liver esterase used in calorimetric biosensor.","authors":"Yi-Hua Zheng,&nbsp;Jun Liu,&nbsp;Yong-Zhi Ma,&nbsp;Yi Xu,&nbsp;Fei Xu,&nbsp;Tse-Chao Hua","doi":"10.3109/10731199.2011.611470","DOIUrl":"https://doi.org/10.3109/10731199.2011.611470","url":null,"abstract":"<p><p>The temperature effects on enzyme activity at the conditions with and without inhibition, operation, and storage were investigated for free esterase and immobilized chicken liver esterase with three methods (adsorption, adsorption-microcapsule, and adsorption-crosslinking) used in a calorimetric biosensor. The results indicated that the temperature had significant effects on the enzyme activity by means of catalytic reaction, irreversible denaturation, mass transfer, and structure deformation of resin. Among all the esterase, the adsorption-crosslinking immobilized esterase had the best performances of enzyme activity retention ratio (EARR), above 96% in operation and 90% in storage on condition of maintaining its response to dichlorvos.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.611470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30018106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Fabrication of capillary-like network in a matrix of water-soluble polymer using poly(methyl methacrylate) microfibers. 用聚甲基丙烯酸甲酯微纤维在水溶性聚合物基质中制备毛细管状网络。
Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-07-06 DOI: 10.3109/10731199.2011.592492
Takayuki Takei, Naoya Kishihara, Hiroyuki Ijima, Koei Kawakami
{"title":"Fabrication of capillary-like network in a matrix of water-soluble polymer using poly(methyl methacrylate) microfibers.","authors":"Takayuki Takei,&nbsp;Naoya Kishihara,&nbsp;Hiroyuki Ijima,&nbsp;Koei Kawakami","doi":"10.3109/10731199.2011.592492","DOIUrl":"https://doi.org/10.3109/10731199.2011.592492","url":null,"abstract":"<p><p>Poly(methyl methacrylate) (PMMA) microfibers were used as a template for development of a capillary-like network in agarose hydrogel. Microfibers with diameter 10-20 μm, which is comparable to the diameter of native capillary vessels, were fabricated using a wet spinning technique. The microfibers were embedded in agarose gel and dissolved by immersing the gel in dichloromethane. The resultant microchannels in the gel had the same diameter as the microfibers, and allowed an aqueous solution to be perfused through the gel. The methodology is promising for fabricating a capillary-like network in tissue engineering scaffolds of various water-soluble polymers.</p>","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2011.592492","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29986985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
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