Artificial cells, blood substitutes, and immobilization biotechnology最新文献

Chitosan-based intragastric delivery of cefuroxime axetil: development and in-vitro evaluation of mucoadhesive approach. 壳聚糖基灌胃给药头孢呋辛酯:黏附方法的发展和体外评价。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-12-01 Epub Date: 2012-07-10 DOI: 10.3109/10731199.2012.696064

Mitesh Nagar, Adhikrao V Yadav

引用次数: 4

Covalent immobilization of trypsin on glutaraldehyde-activated silica for protein fragmentation. 胰酶在戊二醛活化二氧化硅上的共价固定化。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-12-01 Epub Date: 2012-06-06 DOI: 10.3109/10731199.2012.686917

Cenk Daglioglu, Figen Zihnioglu

引用次数: 17

Immune-stimulating potential of cell envelope proteins from Vibrio cholerae associated to chitosan microparticles: an in vitro study. 壳聚糖微粒对霍乱弧菌包膜蛋白免疫刺激作用的体外研究。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-12-01 Epub Date: 2012-09-04 DOI: 10.3109/10731199.2012.696063

Abhimanyu Dev, Roop Narayan Gupta

{"title":"Immune-stimulating potential of cell envelope proteins from Vibrio cholerae associated to chitosan microparticles: an in vitro study.","authors":"Abhimanyu Dev, Roop Narayan Gupta","doi":"10.3109/10731199.2012.696063","DOIUrl":"https://doi.org/10.3109/10731199.2012.696063","url":null,"abstract":"Context: Cholera is a severe diarrheal disease that remains an important cause of illness and death in many parts of the world.Objective: This study has been designed to check the immune-stimulating potential of antigens in their native and associated form as chitosan microparticles in vitro.Material and methods: Chitosan microparticles were prepared by the ionic gelation technique. The cell envelope proteins (CEPs) isolated from Vibrio cholerae were loaded as antigenic material. The prepared microparticles were characterized for their morphology, loading efficiency, particle size, and zeta potential.Results: The average particle size of CEPs-loaded chitosan microparticles was 2.24 µm and the zeta potential of loaded microparticles was less than blank microparticles. The in vitro release studies of CEPs from CEPs-loaded chitosan microparticles exhibited slow and extended release over a period of time. The higher release of cytokine profile, including interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), and interlukin-6 (IL-6), was observed for CEPs-loaded chitosan microparticles in comparison to CEPs as native antigen.Discussion: The particle size of microparticles was within the range for phagocytosis by macropahges, which affects the immunogenicity. The decrease in zeta potential from blank to loaded microparticles further confirms the loading of antigen. The slow and extended release of CEPs provides continuous stimulus of antigen for a longer period of time. The cytokine profiling has shown the advantage of loaded microparticles over native antigen.Conclusion: The in vitro release studies and cytokine profiling strongly suggested that CEPs-associated chitosan microparticles could be a potential candidate for oral vaccination against Vibrio cholerae.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":"40 6","pages":"400-5"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2012.696063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30879597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

In vitro effect of novel β-lactam compounds on xanthine oxidase enzyme activity. 新型β-内酰胺类化合物对黄嘌呤氧化酶活性的体外影响。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-12-01 Epub Date: 2012-06-06 DOI: 10.3109/10731199.2012.678943

Arlinda Bytyqi-Damoni, Hayriye Genç, Mustafa Zengin, Serap Beyaztas, Nahit Gençer, Oktay Arslan

引用次数: 17

The effect of polymerized placenta hemoglobin on renal ischemia/reperfusion injury. 聚合胎盘血红蛋白对肾缺血再灌注损伤的影响。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-12-01 DOI: 10.3109/10731199.2012.696062

Tao Li, Zhenyu Zhang, Daqin Liao, Yanfang Chen, Chengmin Yang, Xuewen Xu, Jin Liu

引用次数: 9

Synthesis of microwave-assisted poly(methyl vinyl ether-co-maleic anhydride)-bovine serum albumin bioconjugates. 微波辅助聚甲基乙烯醚-共马来酸酐-牛血清白蛋白生物偶联物的合成。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-12-01 Epub Date: 2012-04-30 DOI: 10.3109/10731199.2012.678942

Mesut Karahan, Sevecen Tuğlu, Zeynep Mustafaeva

引用次数: 4

Immobilization of anti-aflatoxin B1 antibody by UV polymerization of aniline and aflatoxin B1 detection via electrochemical impedance spectroscopy. 苯胺紫外聚合固定抗黄曲霉毒素B1抗体及电化学阻抗谱检测黄曲霉毒素B1。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-12-01 Epub Date: 2012-09-04 DOI: 10.3109/10731199.2012.696059

Erhan Dinçkaya, Özer Kinik, Mustafa Kemal Sezgintürk, Çağri Altuğ, Aylin Akkoca

引用次数: 6

Purification and characterization of prophenoloxidase from Galleria mellonella L. mellonella原酚氧化酶的纯化及特性研究。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-12-01 Epub Date: 2012-07-10 DOI: 10.3109/10731199.2012.696060

Dudu Demir, Nahit Gençer, Aylin Er

引用次数: 23

Implanted electro-acupuncture electric stimulation improves outcome of stem cells' transplantation in spinal cord injury. 植入式电针电刺激可改善脊髓损伤干细胞移植的预后。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-10-01 Epub Date: 2012-03-02 DOI: 10.3109/10731199.2012.659350

Haichun Liu, Kaiyun Yang, Tao Xin, Wenliang Wu, Yunzhen Chen

{"title":"Implanted electro-acupuncture electric stimulation improves outcome of stem cells' transplantation in spinal cord injury.","authors":"Haichun Liu, Kaiyun Yang, Tao Xin, Wenliang Wu, Yunzhen Chen","doi":"10.3109/10731199.2012.659350","DOIUrl":"https://doi.org/10.3109/10731199.2012.659350","url":null,"abstract":"Spinal cord injury (SCI) is one of the most serious disorders in clinics, and the high disability rate and functional deficits are common issues in patients. Transplantation of bone-marrow-derived mesenchymal stromal cells (BMSCs) into the injured spinal cord is emerging as a novel method in the therapeutics of SCI; however, its application is limited by the poor survival rate of the transplanted cells and low differentiation rate into neurons. Our laboratory recently reported that electrical stimulation (ES) dramatically improves the survival rate of transplanted BMSCs and increases spinal cord functions in animals with spinal cord injury. In this paper, we asked whether implanted electro-acupuncture (iEA) can advance the beneficial effects from the ES treatment in animals with spinal cord injury. We showed that BMSCs transplantation alone resulted in significant functional recovery in animals. Interestingly, iEA with BMSCs treatment induced a significantly higher functional improvement in locomotor functions and SSEP compared to the BMSCs treatment alone. Additionally, we used molecular biology techniques and showed that BMSCs transplantation with iEA treatment significantly increased the number of surviving BMSCs compared to the BMSCs alone group. In conclusion, our experiment showed that the approach of coupling iEA electric stimulation and BMSCs transplantation remarkably promotes functional improvements in animals with spinal cord injury and holds promising potential to treat spinal cord injury in humans.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":"40 5","pages":"331-7"},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2012.659350","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30501883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Immobilization of laccase on polyacrylamide and polyacrylamide - κ - carragennan-based semi-interpenetrating polymer networks. 漆酶在聚丙烯酰胺和聚丙烯酰胺- κ -卡拉胶基半互穿聚合物网络上的固定化。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-10-01 Epub Date: 2012-05-17 DOI: 10.3109/10731199.2012.658469

M Gökgöz, H Altinok

{"title":"Immobilization of laccase on polyacrylamide and polyacrylamide - κ - carragennan-based semi-interpenetrating polymer networks.","authors":"M Gökgöz, H Altinok","doi":"10.3109/10731199.2012.658469","DOIUrl":"https://doi.org/10.3109/10731199.2012.658469","url":null,"abstract":"In this study, laccase enzyme (L) from Agaricus bisporus was immobilized by entrapment into polyacrylamide (PAAm) and semi-interpenetrating polymer networks (semi-IPNs) prepared with either polyacrylamide/κ-carragennan (0.05g) [PAAm/ κ-car (0.05)] or polyacrylamide/κ-carragennan (0.1 g) [PAAm/ κ-car (0.1)]. The optimum pH was 6.0 for free L, 8.0 for PAAm-L, 8.5 for PAAm/κ-car (0.05)-L, and 9.0 for PAAm/κ-car (0.1)-L. The optimum temperature was determined as 45°C for free L and 60°C for all immobilized laccases. After 27 days of storage at 4°C, free enzyme lost its initial activity whereas immobilized enzymes retained 56 % (-)80% of their initial activities. The immobilized samples were used repeatedly 35 times by retaining 28 %-58 % of their initial activity. K(m(app)) values were calculated as 0.088, 0.139, 0.133, and 0.131 mM and Vmax values were found to be 2.83 x 10(-3), 4.51×10(-3), 4.76×10(-3), and 4.97×10(-3) mM min(-1) for free L and PAAm-L, PAAm/κ-car (0.05)-L, and PAAm/κ-car (0.1)-L, respectively.","PeriodicalId":8413,"journal":{"name":"Artificial cells, blood substitutes, and immobilization biotechnology","volume":"40 5","pages":"326-30"},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199.2012.658469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30624903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10