Immobilization of anti-aflatoxin B1 antibody by UV polymerization of aniline and aflatoxin B1 detection via electrochemical impedance spectroscopy.

Erhan Dinçkaya, Özer Kinik, Mustafa Kemal Sezgintürk, Çağri Altuğ, Aylin Akkoca
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引用次数: 6

Abstract

Abstract In the study, we investigated the practicality of the UV polymerization of aniline for anti-aflatoxin B1 antibody immobilization, and utilization of the resulting biosensor in the impedimetric determination of aflatoxin B1. The anti-aflatoxin B 1 antibody was physically immobilized on gold electrodes by UV polymerization of aniline at a fixed wavelength. The biosensor was based on specific interaction anti-aflatoxin B1 – aflatoxin B1 recognition and investigation of this recognition event by electrochemical impedance spectroscopy. A calibration curve was obtained in a linear detection range 1–20 ng/mL aflatoxin B1. Finally, the biosensor was applied to analysis of a real food sample.
苯胺紫外聚合固定抗黄曲霉毒素B1抗体及电化学阻抗谱检测黄曲霉毒素B1。
在本研究中,我们探讨了苯胺紫外聚合用于黄曲霉毒素B1抗体固定化的可行性,以及将所得生物传感器应用于黄曲霉毒素B1的阻抗测定。将抗黄曲霉毒素b1抗体通过苯胺紫外聚合在固定波长的金电极上物理固定。该生物传感器基于抗黄曲霉毒素B1特异性相互作用-识别黄曲霉毒素B1,并通过电化学阻抗谱对该识别事件进行研究。在1 ~ 20 ng/mL黄曲霉毒素B1的线性检测范围内建立了校准曲线。最后,将该生物传感器应用于实际食品样品的分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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