基于生物传感器的蘑菇(Agaricus bisporus)组织匀浆抑制剂测定亚硫酸盐。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-06-30 DOI:10.3109/10731199.2011.585614

Mustafa Kemal Sezgintürk, Erhan Dinçkaya

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引用次数: 2

摘要

本研究的目的是开发一种以双孢蘑菇组织匀浆为基础的生物传感器，用于食品中亚硫酸盐的灵敏和经济测定。该传感器的工作原理是基于亚硫酸盐对蘑菇中多酚氧化酶的抑制作用。用明胶和戊二醛在clark型氧电极上固定化蘑菇组织匀浆。确定了与生物活性层组成和工作条件有关的优化研究。将该生物传感器应用于食品样品中。本文报道的生物传感器已成功用于分析实际食品样品中的食品添加剂亚硫酸盐。

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Sulfite determination by an inhibitor biosensor-based mushroom (Agaricus bisporus) tissue homogenate.

The aim of the study presented here is to develop a biosensor based on mushroom (Agaricus bisporus) tissue homogenate for sensitive and economical determination of sulfite in foods. The working principle of the biosensor is based on an inhibition effect of sulfite on polyphenol oxidases in mushroom. Mushroom tissue homogenate was immobilized by gelatin and glutaraldehyde on a Clark-type oxygen electrode. Some optimization studies related to the bioactive layer components and working conditions were identified. The biosensor was applied to the food samples. The biosensor reported here was successfully allowed to analyze sulfite, which was a food additive in real food samples.

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来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

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6-12 weeks