微胶囊化酿酒酵母柱生物反应器在肾衰竭尿毒症中的潜在应用。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 DOI:10.3109/10731199.2011.597758

Razek Georges Coussa, Sapna Shah, Poonam Jain, Christopher Martoni, Jasmine Bhathena, Meenakshi Malhotra, Satya Prakash

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引用次数: 1

摘要

设计了一种含有活性APA微囊化酵母细胞的新型生物反应器。采用大鼠血浆进行灌注。研究了酵母细胞负载和灌注流速，以最大限度地去除尿素。尿素去除率由5.67±1.34%提高到30.45±0.48%。流速由低到高，尿素去除率由30.46%提高到40.4%。在100%柱载量和高流速下，肌酐和磷酸盐浓度分别降低22%和10%，氨浓度升高58.9% (p < 0.05)。我们的体外灌注研究表明，微囊化酵母细胞可以有效地去除尿素。

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Microencapsulated Saccharomyces cerevisiae column bioreactor for potential use in renal failure uremia.

A novel bioreactor containing viable APA microencapsulated yeast cells was designed. Rat plasma was used for perfusion. Yeast cell loading and perfusion flow rate were studied to maximize urea removal. An increase in column loading from 25% to 100%, increased urea removal from 5.67 ± 1.34% to 30.45 ± 0.48%. An increase in flow rate from low to high, increased urea removal from 30.46% to 40.4%. At 100% column loading and high flow rate, the creatinine and phosphate concentrations decreased by 22% and 10%, respectively, while ammonia concentrations increased by 58.9% (p < 0.05). Our in-vitro perfusion study demonstrates that microencapsulated yeast cells can remove urea efficiently.

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来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

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审稿时长

6-12 weeks