多血红蛋白-超氧化物歧化酶-过氧化氢酶-碳酸酐酶:一种新型的基于生物技术的血液替代品，可运输氧气和二氧化碳，也可作为抗氧化剂。

Artificial cells, blood substitutes, and immobilization biotechnology Pub Date : 2012-02-01 Epub Date: 2011-06-21 DOI:10.3109/10731199.2011.582041

Yuzhu Bian, Zhixia Rong, Thomas Ming Swi Chang

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引用次数: 4

摘要

多血红蛋白-超氧化物歧化酶-过氧化氢酶-碳酸酐酶(PolyHb-SOD-CAT-CA)是一种治疗性抗氧化剂，也运输氧气和二氧化碳。这是通过使用戊二醛将Hb与SOD、CAT和CA交联而形成的。从红细胞(RBC)中交联无基质Hb可将CA活性降低至55%。添加更多的CA导致制剂具有与RBC相同的CA活性。当二氧化碳转化为碳酸时，复合物中的多聚血红蛋白起到缓冲作用，防止pH值发生大的变化。然后，我们制备并优化了一种新型的PolyHb-SOD-CAT-CA，一种治疗性抗氧化剂，也可以运输氧气和二氧化碳。

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Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant.

Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma-free Hb from red blood cell (RBC) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide.

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来源期刊

Artificial cells, blood substitutes, and immobilization biotechnology 工程技术-材料科学：生物材料

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6-12 weeks