Archives of Insect Biochemistry and Physiology最新文献

{"title":"Response of wild aquatic insect communities to thermal variation through comparative landscape transcriptomics","authors":"Maribet Gamboa,&nbsp;Yusuke Gotoh,&nbsp;Arnelyn Doloiras-Laraño,&nbsp;Kozo Watanabe","doi":"10.1002/arch.22137","DOIUrl":"10.1002/arch.22137","url":null,"abstract":"<p>Fluctuations in temperature are recognized as a potent driver of selection pressure, fostering genomic variations that are crucial for the adaptation and survival of organisms under selection. Notably, water temperature is a pivotal factor influencing aquatic organism persistence. By comprehending how aquatic organisms respond to shifts in water temperature, we can understand their potential physiological adaptations to environmental change in one or multiple species. This, in turn, contributes to the formulation of biologically relevant guidelines for the landscape scale transcriptome profile of organisms in lotic systems. Here, we investigated the distinct responses of seven stream stonefly species, collected from four geographical regions across Japan, to variations in temperature, including atmospheric and water temperatures. We achieved this by assessing the differences in gene expression through RNA-sequencing within individual species and exploring the patterns of community-genes among different species. We identified 735 genes that exhibited differential expressions across the temperature gradient. Remarkably, the community displayed expression levels differences of respiration and metabolic genes. Additionally, the diversity in molecular functions appeared to be linked to spatial variation, with water temperature differences potentially contributing to the overall functional diversity of genes. We found 22 community-genes with consistent expression patterns among species in response to water temperature variations. These genes related to respiration, metabolism and development exhibited a clear gradient providing robust evidence of divergent adaptive responses to water temperature. Our findings underscore the differential adaptation of stonefly species to local environmental conditions, suggesting that shared responses in gene expression may occur across multiple species under similar environmental conditions. This study emphasizes the significance of considering various species when assessing the impacts of environmental changes on aquatic insect communities and understanding potential mechanisms to cope with such changes.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ultraviolet on the environmental adaptability of Megalurothrips usitatus (Thysanoptera: Thripidae)","authors":"Haifeng Jin,&nbsp;Chaozheng Wang,&nbsp;Wayne Jiang,&nbsp;Xia Li,&nbsp;Fen Li,&nbsp;Shaoying Wu","doi":"10.1002/arch.22141","DOIUrl":"10.1002/arch.22141","url":null,"abstract":"<p><i>Megalurothrips usitatus</i> Bagnall, an important pest of bean plants, is primarily managed with synthetic insecticides. <i>M. usitatus</i> has developed considerable resistance to various insecticides in multiple cowpea-growing areas in Hainan Province, China, posing challenges to its control in the field. Light control technology is a potentially effective physical control method for <i>M. usitatus</i>. The vision of thrips is highly sensitive to UV light, whereas other biological characteristics remain unknown. Therefore, this study evaluated the effects of ultraviolet light on the biological characteristics of <i>M. usitatus</i>. Results showed that the egg, larval, and pupal stages of <i>M. usitatus</i> were significantly shortened, and the emergence rate (79.59%) and adult survival rate (77.95%) were reduced under a devoid of UV light environment (UV−), compared with the full-spectrum light (control treatment group, CK) (<i>p</i> &lt; 0.05). However, the single spawning quantity and total amount of spawning were significantly higher, and the sex ratio (57%) was the highest under UV− (<i>p</i> &lt; 0.05). Single UV light (UV+) only affected the pupation rate. Also, the antioxidant enzymes, polyphenol oxidase, superoxide dismutase (SOD), and peroxidase activities were significantly and negatively correlated with the progression of generations under UV−, whereas catalase and SOD activities were significantly and positively correlated with the progression of generations under UV+. The UV− light conditions significantly interfered with the behavior selection of <i>M. usitatus</i>. The results of this study showed that the adaptability of <i>M. usitatus</i> populations would be greatly reduced in the absence of ultraviolet light, providing a theoretical basis for the control of <i>M. usitatus</i> populations.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the temperature adaptation of three rubber tree pest mites based on their two-sex life table","authors":"Weihua Wang,&nbsp;Sanlian Wan,&nbsp;Lijiu Zheng,&nbsp;Fangping Zhang,&nbsp;Junyu Chen","doi":"10.1002/arch.22138","DOIUrl":"10.1002/arch.22138","url":null,"abstract":"<p>To determine the optimal temperature range for the development and reproduction of three spider mites (<i>Eotetranychus sexmaculatus</i>, <i>Eotetranychus orientalis</i>, and <i>Oligonychus biharensis</i>), this study investigated their developmental period, survival rate, lifespan, and reproduction under five temperatures, 21, 24, 27, 30, and 33°C, to predict and control in the field. With the gathered data, a two-sex life table was constructed for each of them. The results revealed that as the temperature increased, both <i>O. biharensis</i> and <i>E. orientalis</i> displayed a gradual reduction in their generation period. Furthermore, an inverse relationship was observed between lifespan and temperature for all three spider mite species. When examining the survival rates at varying temperatures, <i>E. sexmaculatus</i> exhibited the highest rate (98%) at 33°C, while <i>E. orientalis</i> and <i>O. biharensis</i> demonstrated their highest survival rates at 24°C, reaching 90% and 100% respectively. Regarding reproduction, <i>O. biharensis</i> displayed the highest oviposition rates at 30°C with an average of 17.45 eggs per individual. Conversely, <i>E. sexmaculatus</i> and <i>E. orientalis</i> exhibited the highest oviposition rates at 33°C, averaging at 15.22 and 21.38 eggs per individual respectively. Significantly higher intrinsic growth rates were observed for <i>O. biharensis</i> and <i>E. orientalis</i> at 33°C, with rates of 0.22 and 0.26 respectively. In contrast, <i>E. sexmaculatus</i> demonstrated the highest intrinsic growth rate at 27°C. The temperature of 27°C was more suitable for the growth of the <i>E. sexmaculatus</i>, while 33°C was the optimal temperature for the <i>E. orientalis</i> and <i>O. biharensis</i>. The current findings provide valuable guidance for the control and prevention of these three spider mites.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological function of Bombyx Toll9-2 in the silkworm (Bombyx mori) larval midgut: Activation by Escherichia coli/lipopolysaccharide and regulation of growth","authors":"Jisheng Liu,&nbsp;Weifeng Yang,&nbsp;Wenli Liao,&nbsp;Yanling Huang,&nbsp;Weijian Chen,&nbsp;Xiaoling Bu,&nbsp;Shiyi Huang,&nbsp;Wanyi Jiang,&nbsp;Luc Swevers","doi":"10.1002/arch.22130","DOIUrl":"10.1002/arch.22130","url":null,"abstract":"<p>Toll receptors are important regulators of insects' innate immune system which, upon binding of pathogen molecules, activate a conserved signal transduction cascade known as the Toll pathway. RNA interference (RNAi) is a powerful tool to study the function of genes via reverse genetics. However, due to the reported refractory of RNAi efficiency in lepidopteran insects, successful reports of silencing of Toll receptors in the silkworm <i>Bombyx mori</i> have not been reported yet. In this study, a Toll receptor of the silkworm <i>Bombyx Toll9-2</i> (<i>BmToll9-2</i>) was cloned and its expression and function were analyzed. The results showed that <i>BmToll9-2</i> contains an ectodomain (ECD) with a signal peptide and nine leucine-rich repeats, a transmembrane helix, and a cytoplasmic region with a Toll/interleukin-1 domain. Phylogenetic analysis indicates that BmToll9-2 clusters with other insect Toll9 receptors and mammalian Toll-like receptor 4. Oral infection of exogenous pathogens showed that the Gram-negative bacterium <i>Escherichia coli</i> and its main cell wall component lipopolysaccharide (LPS), as well as the Gram-positive bacterium <i>Staphylococcus aureus</i> and its main cell wall component peptidoglycan, significantly induce <i>BmToll9-2</i> expression in vivo. LPS also induced the expression of <i>BmToll9-2</i> in BmN4 cells in vitro. These observations indicate its role as a sensor in the innate immunity to exogenous pathogens and as a pathogen-associated receptor that is responsive to LPS. RNAi of <i>BmToll9-2</i> was effective in the midgut and epidermis. RNAi-mediated knock-down of <i>BmToll9-2</i> reduced the weight and growth of the silkworm. Bacterial challenge following RNAi upregulated the expression of <i>BmToll9-2</i> and rescued the weight differences of the silkworm, which may be related to its participation in the immune response and the regulation of the microbiota in the midgut lumen of the silkworm larvae.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Honeybee (Apis mellifera L.) pollination enhances the yield and flavor quality of kiwifruit","authors":"Quanzhi Ji,&nbsp;Xue Wang,&nbsp;Ting Huang,&nbsp;Xinyu Wang,&nbsp;Yazhou Zhao","doi":"10.1002/arch.22139","DOIUrl":"10.1002/arch.22139","url":null,"abstract":"<p>Pollination is essential for achieving high yields and enhancing the quality of kiwifruit cultivation, both of which significantly influence growers' interests and consumers' preferences. However, compared to studies on yield, there are fewer studies exploring the impact of pollination methods on the flavor of kiwifruit <i>Actinidia chinensis</i> Planchon. This study examined the effects of bee (<i>Apis mellifera</i> L.) pollination and artificial pollination on the yield and flavor of kiwifruit in the main producing areas of China. Compared with those pollinated artificially, bee-pollinated kiwifruit exhibited a greater fruit set rate, heavier fruit weight, and greater number of seeds. Notably, the number of seeds was positively correlated with fruit weight in bee-pollinated kiwifruit, whereas no such correlation was detected in artificially pollinated fruit. Bee pollination not only enhanced the yield but also improved the flavor of kiwifruit. Specifically, bee-pollinated kiwifruit contained higher levels of sucrose and lower concentrations of glucose and fructose, while the acid content was less affected by pollination methods. Furthermore, significant differences were observed in the volatile organic compound (VOC) levels in kiwifruit subjected to different pollination treatments, with bee-pollinated fruit exhibiting a superior flavor. Our findings provide new insights into the beneficial role of bee pollination in enhancing kiwifruit yield and quality, underscoring the crucial importance of bees in kiwifruit pollination.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition and abundance of midgut plasma membrane proteins in two major hemipteran vectors of plant viruses, Bemisia tabaci and Myzus persicae","authors":"Jaime Jiménez,&nbsp;Ruchir Mishra,&nbsp;Xinyue Wang,&nbsp;Ciara M. Magee,&nbsp;Bryony C. Bonning","doi":"10.1002/arch.22133","DOIUrl":"10.1002/arch.22133","url":null,"abstract":"<p>Multiple species within the order Hemiptera cause severe agricultural losses on a global scale. Aphids and whiteflies are of particular importance due to their role as vectors for hundreds of plant viruses, many of which enter the insect via the gut. To facilitate the identification of novel targets for disruption of plant virus transmission, we compared the relative abundance and composition of the gut plasma membrane proteomes of adult <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) and <i>Myzus persicae</i> (Hemiptera: Aphididae), representing the first study comparing the gut plasma membrane proteomes of two different insect species. Brush border membrane vesicles were prepared from dissected guts, and proteins extracted, identified and quantified from triplicate samples via timsTOF mass spectrometry. A total of 1699 <i>B. tabaci</i> and 1175 <i>M. persicae</i> proteins were identified. Following bioinformatics analysis and manual curation, 151 <i>B. tabaci</i> and 115 <i>M. persicae</i> proteins were predicted to localize to the plasma membrane of the gut microvilli. These proteins were further categorized based on molecular function and biological process according to Gene Ontology terms. The most abundant gut plasma membrane proteins were identified. The ten plasma membrane proteins that differed in abundance between the two insect species were associated with the terms “protein binding” and “viral processes.” In addition to providing insight into the gut physiology of hemipteran insects, these gut plasma membrane proteomes provide context for appropriate identification of plant virus receptors based on a combination of bioinformatic prediction and protein localization on the surface of the insect gut.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.22133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the contribution of the salivary gland and midgut to digestion in the swede midge (Contarinia nasturtii) through a genomics-guided approach","authors":"Boyd A. Mori,&nbsp;Cathy Coutu,&nbsp;Martin A. Erlandson,&nbsp;Dwayne D. Hegedus","doi":"10.1002/arch.22135","DOIUrl":"10.1002/arch.22135","url":null,"abstract":"<p>The larvae of <i>Contarinia nasturtii</i> (Kieffer) (Diptera: Cecidomyiidae), the swede midge, targets the meristem of brassica crops where they induce the formation of galls and disrupt seed and vegetable production. Previously, we examined the salivary gland transcriptome of newly-hatched first instar larvae as they penetrated the host and initiated gall formation. Here we examine the salivary gland and midgut transcriptome of third instar larvae and provide evidence for cooperative nutrient acquisition beginning with secretion of enzymes and feeding facilitators followed by gastrointestinal digestion. Sucrose, presumably obtained from the phloem, appeared to be a major nutrient source as several α-glucosidases (sucrases, maltases) and β-fructofuranosidases (invertases) were identified. Genes encoding β-fructofuranosidases/invertases were among the most highly expressed in both tissues and represented two distinct gene families that may have originated via horizontal gene transfer from bacteria. The importance of the phloem as a nutrient source is underscored by the expression of genes encoding regucalcin and ARMET (arginine-rich mutated in early stages of tumor) which interfere with calcium signalling and prevent sieve tube occlusion. Lipids, proteins, and starch appear to serve as a secondary nutrient sources. Genes encoding enzymes involved in the detoxification of glucosinolates (myrosinases, arylsulfatases, and glutathione-S-transferases) were expressed indicative of Brassicaceae host specialization. The midgut expressed simple peritrophins and mucins typical of those found in Type II peritrophic matrices, the first such description for a gall midge.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.22135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silence of Aminopeptidase N 2 gene reveals the trade-offs for acquiring Cry1Ac resistance in Plutella xylostella","authors":"Zhuohong Lv,&nbsp;Shuwen Yu,&nbsp;Yafei Zhao,&nbsp;Zhongxia Yang","doi":"10.1002/arch.22131","DOIUrl":"10.1002/arch.22131","url":null,"abstract":"<p><i>Bacillus thuringiensis</i> (Bt) is widely used as a biopesticide worldwide. To date, at least eight pest species have been found to be resistant to Bt in the field. As the first pest that was reported having resistance to Bt in the field, considerable research has been done on the mechanisms of Bt resistance in <i>Plutella xylostella</i>. However, whether the acquisition of Bt resistance by <i>P. xylostella</i> comes at a fitness cost is also a valuable question. In this study, <i>Aminopeptidase-N 2</i> (<i>APN2</i>), a Cry toxin receptor gene of <i>P. xylostella</i>, was knocked down by RNA interference, resulting in improved resistance to Cry1Ac. It was also found that larval mortality of <i>APN2</i> knockdown <i>P. xylostella</i> was significantly higher than that of the control, while the pupation rate, pupal weight, eclosion rate, fecundity (egg/female), hatchability, and female adult longevity were significantly lower in <i>APN2</i> knockdown <i>P. xylostella</i> than in the control. These results illustrate that if Cry1Ac resistance was obtained only through the reduction of <i>APN2</i> expression, <i>P. xylostella</i> would need to incur some fitness costs for it.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and characterization of the histone variant gene H2A.Z in Bombyx mori","authors":"Jun Mei,&nbsp;Kunling Xu,&nbsp;Yuyi Huang,&nbsp;Jingwei Zhang,&nbsp;Qitao Qian,&nbsp;Jia Dong,&nbsp;Fudan Tong,&nbsp;Wei Yu,&nbsp;Meng Miao","doi":"10.1002/arch.22136","DOIUrl":"10.1002/arch.22136","url":null,"abstract":"<p>H2A.Z, the most evolutionarily conserved variant of histone H2A, plays a pivotal role in chromatin remodeling and contributes significantly to gene transcription and genome stability. However, the role of H2A.Z in the silkworm (<i>Bombyx mori</i>) remains unclear. In this study, we cloned the <i>BmH2A.Z</i> from <i>B. mori</i>. The open reading frame of <i>BmH2A.Z</i> is 390 bp, encoding 129 amino acids, with a confirmed molecular weight of 13.4 kDa through prokaryotic expression analysis. Sequence analysis revealed that BmH2A.Z has a conserved H2A.Z domain and is closely related to the systemic evolution of other known H2A.Zs. The expression profile of <i>BmH2A.Z</i> at various developmental stages of the <i>B. mori</i> exhibited the highest expression level in the 1st instar, followed by the grain stage and the 2nd instar, and the lowest expression level in the moth. The highest transcript level of <i>BmH2A.Z</i> was observed in the head, with relatively lower levels detected in the blood than in the other tissues under consideration. In addition, the upregulation of BmH2A.Z resulted in the amplified expression of <i>B. mori</i> nucleopolyhedrovirus (BmNPV) genes, thus facilitating the proliferation of BmNPV. This study establishes a foundation for investigating the role of BmH2A.Z in <i>B. mori</i> and its participation in virus-host interactions.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Depletion of lipid storage droplet-1 delays endoreplication progression and induces cell death in Drosophila salivary gland","authors":"Yen D. H. Nguyen,&nbsp;Tuan L. A. Pham,&nbsp;Taisei Nishihara,&nbsp;Kaeko Kamei,&nbsp;Duy Binh Tran","doi":"10.1002/arch.22132","DOIUrl":"10.1002/arch.22132","url":null,"abstract":"<p>Perilipins are evolutionarily conserved from insects to mammals. <i>Drosophila</i> lipid storage droplet-1 (LSD-1) is a lipid storage droplet membrane surface-binding protein family member and a counterpart to mammalian perilipin 1 and is known to play a role in lipolysis. However, the function of LSD-1 during specific tissue development remains under investigation. This study demonstrated the role of LSD-1 in salivary gland development. Knockdown of <i>Lsd-1</i> in the salivary gland was established using the GAL4/UAS system. The third-instar larvae of knockdown flies had small salivary glands containing cells with smaller nuclei. The null mutant <i>Drosophila</i> also showed the same phenotype. The depletion of LSD-1 expression induced a delay of endoreplication due to decreasing CycE expression and increasing DNA damage. <i>Lsd-1</i> genetically interacted with <i>Myc</i> in the third-instar larvae. These results demonstrate that LSD-1 is involved in cell cycle and cell death programs in the salivary gland, providing novel insight into the effects of LSD-1 in regulating salivary gland development and the interaction between LSD-1 and Myc.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.22132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}