Archives of Insect Biochemistry and Physiology最新文献_第5页

Insect Reproductive Behavior: Key Genes and Their Mechanisms of Action 昆虫生殖行为：关键基因及其作用机制。

IF 2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-06-24 DOI: 10.1002/arch.70181

Wang-He Zhang, Qiu-Lang Zhang, Si-Jia Chang

{"title":"Insect Reproductive Behavior: Key Genes and Their Mechanisms of Action","authors":"Wang-He Zhang, Qiu-Lang Zhang, Si-Jia Chang","doi":"10.1002/arch.70181","DOIUrl":"10.1002/arch.70181","url":null,"abstract":"<div>\u0000 \u0000 Reproductive behavior refers to a series of activities involved in animal reproduction, including intersexual attraction, courtship, mating, oviposition, and parental care. It is the most common behavior in the biological world and plays a vital role in population propagation. The in-depth study of insect reproductive behavior is fundamental to understanding species' sexual selection and evolution, with important practical significance for pest control and the utilization of beneficial insects. Reproductive behavior is a complex biological process regulated by genes. Research on such genes can offer new ideas and target genes for novel pest control strategies based on reproductive behavior, pheromones, and RNAi. Presently, control methods established using insect sex pheromones and mating behavior patterns have been successfully applied in monitoring and controlling some significant agricultural pests. This review synthesizes current knowledge on genes regulating insect reproductive behavior from three interconnected perspectives: intrasexual regulation (with emphasis on neuropeptides such as tachykinins and natalisin), intersexual regulation (focusing on male accessory gland proteins and female post‑mating responses), and sex pheromone synthesis control (including both female‑autonomous PBAN/PBANR signaling and male‑derived pheromonostatic factors). While major progress has been made in model insects, functional validation in non‑model species and the integration of multi‑omics data remain key challenges. We also discuss emerging tools such as CRISPR/Cas9 and bacterial‑mediated RNAi, which hold promise for translating mechanistic insights into behavior‑based pest management strategies.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"122 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune Response and Gut Microbiota Shift in the Red Palm Weevil (Rhynchophorus ferrugineus) Infected With Entomopathogenic Fungus, Beauveria bassiana, Reveal Host-Pathogen Interactions 感染球孢白僵菌的红棕榈象鼻虫的免疫反应和肠道菌群变化揭示了宿主-病原体的相互作用

IF 2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-06-23 DOI: 10.1002/arch.70183

Tzu-Hao Yang, Fang-Min Chang, Pin-Chang Chen, Rameshwor Pudasaini, Hsiao-Pei Lu, Yu-Shin Nai

{"title":"Immune Response and Gut Microbiota Shift in the Red Palm Weevil (Rhynchophorus ferrugineus) Infected With Entomopathogenic Fungus, Beauveria bassiana, Reveal Host-Pathogen Interactions","authors":"Tzu-Hao Yang, Fang-Min Chang, Pin-Chang Chen, Rameshwor Pudasaini, Hsiao-Pei Lu, Yu-Shin Nai","doi":"10.1002/arch.70183","DOIUrl":"10.1002/arch.70183","url":null,"abstract":"The red palm weevil (RPW), Rhynchophorus ferrugineus (Oliver), is a major pest of palm plants. Entomopathogenic fungi (EPF) are considered promising biocontrol agents against RPW. This study investigated the changes in immune-related gene expression and gut microbiota of RPW larvae infected with Beauveria bassiana (Bb-NCHU-155). In infected larvae, fungal genome copy numbers in the midgut and hindgut were lower than those detected in the fat body, suggesting that infection primarily occurs via the cuticle rather than the digestive tract. Immune-related gene expression in the fat body increased steadily from 3 days post-inoculation (dpi), reflecting a typical host response, whereas digestive tissues exhibited fluctuating patterns. The midgut showed peak induction of C-type lysozyme at 3 dpi and C-type lectin at 6 dpi, while the hindgut displayed the highest expression of serine protease-like protein at 3 dpi. Despite an overall downregulation of immune-related genes, these results indicate tissue-specific immune responses. Although B. bassiana may not infect hosts through the digestive system, gut microbiota composition differed significantly between infected and control groups, with higher relative abundances of Acetobacteraceae, Lactobacillaceae, and Streptococcaceae in the infected larvae. These shifts co-varied with the expression of immune-related genes, such as defensin and C-type lectin, suggesting potential functional links between gut microbiota and host immunity. This study provides fundamental insights into the effects of EPF on gut microbiota and immune gene expression in RPW, supporting further research into the complex interactions underlying microbial control.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"122 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.70183","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bombyx mori Trehalase 2 Activates Energy Metabolism to Resist Fenpropathrin in Silkworm, Bombyx mori 家蚕海藻酶2激活能量代谢抵抗甲氰菊酯。

IF 2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-06-18 DOI: 10.1002/arch.70174

Kai-yi Zheng, Jia-wei Jiang, Yao-ting Zhang, Li-ming Bu, Xiao-yu Fu, Jia-meng Yang, Xue-yang Wang, Zuo-min Shao, Xu-wei Zhu

{"title":"Bombyx mori Trehalase 2 Activates Energy Metabolism to Resist Fenpropathrin in Silkworm, Bombyx mori","authors":"Kai-yi Zheng, Jia-wei Jiang, Yao-ting Zhang, Li-ming Bu, Xiao-yu Fu, Jia-meng Yang, Xue-yang Wang, Zuo-min Shao, Xu-wei Zhu","doi":"10.1002/arch.70174","DOIUrl":"10.1002/arch.70174","url":null,"abstract":"<div>\u0000 \u0000 The extensive use of pesticides poses a significant threat to sericulture, yet effective control strategies are still limited. Trehalose, the primary sugar in insect hemolymph, is a key player in abiotic stress responses and its metabolism is regulated by trehalase. Despite this, the role of the Bombyx mori trehalase 2 (BmTreh2) in the response to the pyrethroid insecticide remains unexplored. In this study, comparative analysis revealed significant differences in BmTreh2 expression among silkworm (Bombyx mori) strains with varying resistance levels to fenpropathrin, suggesting a potential role of this gene in fenpropathrin resistance. At the cellular level, overexpression of BmTreh2 increased tolerance to fenpropathrin, whereas RNAi-mediated knockdown of BmTreh2 decreased cell viability under fenpropathrin exposure. These results demonstrate that BmTreh2 plays a critical role in mediating fenpropathrin resistance. Furthermore, correlation analysis between BmTreh2 and key energy metabolism-related genes, including lactate dehydrogenase (LDH), alanine aminotransferase (ALT), malate dehydrogenase (MDH), and isocitrate dehydrogenase (IDH), revealed a significant positive correlation with MDH and IDH expression, and a negative correlation with LDH and ALT. This study elucidates key molecular mechanisms underlying silkworm resistance to fenpropathrin, establishing a theoretical framework for molecular breeding of resistant silkworm strains and informing integrated pest management strategies.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"122 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148275883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Energy Metabolism-Mediated Heat Acclimation Enhances Reproductive and Migratory Capacities of Cnaphalocrocis medinalis 能量代谢介导的热驯化提高了稻纵卷叶蝉的繁殖和迁徙能力。

IF 2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-06-15 DOI: 10.1002/arch.70179

Jia-Rong Li, Xiang-Dong Liu

{"title":"Energy Metabolism-Mediated Heat Acclimation Enhances Reproductive and Migratory Capacities of Cnaphalocrocis medinalis","authors":"Jia-Rong Li, Xiang-Dong Liu","doi":"10.1002/arch.70179","DOIUrl":"10.1002/arch.70179","url":null,"abstract":"<div>\u0000 \u0000 Migrants rely on habitat shifts to adapt to changing environments, yet how migratory insect populations cope with global warming remains elusive. Here, we illustrated changes in heat tolerance, reproduction, flight performance, and energy metabolism pathways of a migratory insect Cnaphalocrocis medinalis, a serious pest of rice, induced by heat acclimation, which enhance the capacity to buffer further warming. Compared to natural populations, adults from the heat-acclimated strain exhibited longer heat knockdown time, higher critical thermal maxima, shorter pre-oviposition period, extended oviposition period, and increased fecundity under heat stress. Heat acclimation promoted the take-off behavior at an extremely high temperature and flight distance of adults at normal temperature, and induced a greater number of differentially expressed genes of larvae when exposed to high temperature, particularly genes associated with energy metabolisms. Gene sets related to glycolysis/gluconeogenesis, tricarboxylic acid cycle, oxidative phosphorylation, and pentose phosphate pathway were markedly upregulated in the heat-acclimated larvae. The insulin receptor gene of adults was downregulated in the heat-acclimated strain at normal temperature but significantly upregulated under heat stress which may be involved in energy regulation for migration, reproduction, and heat tolerance. Collectively, our findings demonstrate that migratory populations have an extraordinary capacity to buffer heat stress through energy metabolism-mediated acclimation driven by global warming.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"122 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148257107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imidazole-Modified Graphene Quantum Dots Enhance RNAi Efficiency in Spodoptera litura (Lepidoptera: Noctuidae): A Study Using Cuticular Protein Gene Silencing 咪唑修饰的石墨烯量子点增强斜纹夜蛾（鳞翅目：夜蛾科）的RNAi效率：利用表皮蛋白基因沉默的研究。

IF 1.9 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-06-07 DOI: 10.1002/arch.70176

Fei-Feng Wang, Yuan-Yuan Huang, Peng Qin, Lu-Lu Dong, Jun Pan, You-Wei Xi, Lin Yu, Bao-Li Qiu, Jian-Yu Meng, Wen Sang

{"title":"Imidazole-Modified Graphene Quantum Dots Enhance RNAi Efficiency in Spodoptera litura (Lepidoptera: Noctuidae): A Study Using Cuticular Protein Gene Silencing","authors":"Fei-Feng Wang, Yuan-Yuan Huang, Peng Qin, Lu-Lu Dong, Jun Pan, You-Wei Xi, Lin Yu, Bao-Li Qiu, Jian-Yu Meng, Wen Sang","doi":"10.1002/arch.70176","DOIUrl":"10.1002/arch.70176","url":null,"abstract":"<div>\u0000 \u0000 RNA interference (RNAi) serves as a crucial tool for gene function research and pest control. Nevertheless, its application in lepidopteran insects is restricted by the low efficiency of gene silencing. Spodoptera litura (Lepidoptera: Noctuidae), a globally-distributed polyphagous agricultural pest, has developed multiple strategies to resist control measures. This situation highlights the urgent need for innovative strategies to promote functional genomics and molecular target characterization studies. In this study, a novel cuticular protein SlCP was identified, which is vital for epidermal development and larval survival. Silencing of SlCP gave rise to epidermal contraction, a reduction in body size, a decrease in feed intake, and larval mortality. To address the problem of low RNAi efficiency, imidazole-modified graphene quantum dots (IGQDs) were utilized as nanocarriers for the delivery of dsSlCP. IGQD mediated delivery led to a 4.65-fold greater reduction in SlCP transcript levels compared with naked double-stranded RNA and elevated larval mortality from 38.00% to 56.00%. These findings establish that SlCP functions in epidermal development and present a nanomaterial-assisted strategy that enhances RNAi delivery efficacy in S. litura.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"122 2","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148197231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-Mediated Knockout of White Gene Produces Eye Color Mutants in the Southern Green Stinkbug, Nezara viridula CRISPR/ cas9介导的白色基因敲除在南方绿臭虫Nezara病毒中产生眼睛颜色突变。

IF 1.9 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-06-07 DOI: 10.1002/arch.70175

Karthi Sengodan, Chen Xian, Paul Shirk, Jeff Howell, Anil Kumar Moola, Subba Reddy Palli

引用次数: 0

Differential Expression of Dopamine-Related Genes Across Developmental and Parental Stages in the Burying Beetles, Nicrophorus orbicollis 埋虫多巴胺相关基因在发育和亲本阶段的差异表达

IF 1.9 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-06-01 DOI: 10.1002/arch.70177

Stefania Carmen Panaitof, Marrwa Mohamed Kermagi

{"title":"Differential Expression of Dopamine-Related Genes Across Developmental and Parental Stages in the Burying Beetles, Nicrophorus orbicollis","authors":"Stefania Carmen Panaitof, Marrwa Mohamed Kermagi","doi":"10.1002/arch.70177","DOIUrl":"https://doi.org/10.1002/arch.70177","url":null,"abstract":"<div>\u0000 \u0000 Dopamine has diverse roles in the regulation of reproduction and social behaviors in insects. In the burying beetle, Nicrophorus orbicollis, a species with extended care, our previous work has shown that brain dopamine levels increase significantly after 24 h of care. It is not known, however, whether this increase arises from changes in the expression of genes responsible for dopamine biosynthesis and/or degradation. To that end, we quantified transcript levels of tyrosine hydroxylase (Noth); aromatic amino acid decarboxylase (Noaadc), and dopamine N-acetyltransferase (Nodnat) in head tissue of female burying beetles across four developmental and parental stages: newly-emerged, sexually mature, 1-day parental, and 6-day parental. Surprisingly, transcript levels of Noth, encoding the rate-limiting enzyme, did not increase after 24 h of care relative to non-breeding controls. Moreover, expression of Noaadc, encoding the enzyme involved in the final step of dopamine synthesis, showed a striking, albeit not statistically significant, reduction in actively parenting females at 24 h of care, with transcript levels returning to pre-breeding levels by 6 days of care. In contrast, expression of both Noth and Nodnat was significantly elevated in newly emerged females compared with reproductively mature and parental stages, revealing a pattern of transcriptional upregulation associated with early development, rather than active parenting. The previously observed increase in dopamine during early parenting is thus unlikely to be driven by transcriptional upregulation of biosynthetic genes; post-transcriptional mechanisms, including altered release, turnover, or synaptic signaling, may instead contribute to neuromodulatory changes associated with parental care in burying beetles.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"122 2","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148103398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 1.9 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-05-27

引用次数: 0

IF 1.9 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-05-26

引用次数: 0

IF 1.9 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2026-05-26

引用次数: 0