Aquaculture International最新文献

{"title":"Effects of high density on behavior, physiology and disease resistance of the sea cucumber Apostichopus japonicus under stress conditions","authors":"Ruihuan Tian,&nbsp;Guo Wu,&nbsp;Huiyan Wang,&nbsp;Yunjie Yang,&nbsp;Sihan Wang,&nbsp;Chong Zhao","doi":"10.1007/s10499-024-01808-6","DOIUrl":"10.1007/s10499-024-01808-6","url":null,"abstract":"<div><p>High stocking density is widely adopted due to the efficient water utilization in sea cucumber aquaculture. However, bacterial infection challenge and acute temperature decrease are common and inevitable problems in the aquaculture of sea cucumbers. This study investigated the impacts of high density on survival, behavioral and physiological response of <i>Apostichopus japonicus</i> under stress conditions (bacterial infection challenge and an acute temperature decrease). In experiment II, our results showed that morbidity significantly increased in <i>A</i>. <i>japonicus</i> and that feeding behavior significantly reduced in asymptomatic individuals at high density after bacterial challenge. However, foraging behavior was not significantly affected in asymptomatic individuals. These results suggest that high density probably affects the survival rate of sea cucumbers after the SUS challenge and inhibits feeding behavior of asymptomatic individuals. Moreover, in experiment III, righting and feeding behaviors were significantly inhibited in <i>A</i>. <i>japonicus</i> at high density after acute temperature decrease. However, crawling behavior and feeding preferences were not significantly affected in <i>A</i>. <i>japonicus</i>. These results indicated that high density increased the susceptibility to acute temperature decrease stress in <i>A</i>. <i>japonicus</i>. In addition, in experiment I, we further found that superoxide dismutase (SOD) activity and the level of pyruvate kinase (PK) significantly increased in <i>A</i>. <i>japonicus</i> at high density. Increased SOD and PK are probably the important reasons that cause a decreased stress resistance, when sea cucumbers are exposed to bacterial infection or acute temperature decrease. In conclusion, our study clarified the influences of increased stocking density on survival, behavioral and physiological responses of <i>A</i>. <i>japonicus</i> under stress conditions. We further revealed the possible reasons why high density affects the survival, behavior, and physiology in <i>A</i>. <i>japonicus</i> under stress.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of using kidney bean (Phaseolus vulgaris L) protein hydrolysate as a plant protein supplement in Nile tilapia (Oreochromis niloticus) diets: effects on growth, hematology, histology, and blood biochemical parameters","authors":"Shimaa A. Amer,&nbsp;Rowida E. Ibrahim,&nbsp;Elsayed M. Younis,&nbsp;Abdel-Wahab A. Abdel-Warith,&nbsp;Ali Osman,&nbsp;Simon J. Davies,&nbsp;Medhat S. Shakweer","doi":"10.1007/s10499-024-01727-6","DOIUrl":"10.1007/s10499-024-01727-6","url":null,"abstract":"<div><p>Searching for protein sources for fish diet formulations has become essential. So, this investigation evaluated the impacts of kidney bean protein hydrolysate (KPH) (<i>Phaseolus vulgaris</i> L) as a new plant protein source in the diets of Nile tilapia, <i>Oreochromis niloticus</i> (<i>O. niloticus</i>), on the fish growth, hematology, biochemistry, antioxidant status, and tissue histomorphology. Two hundred fingerlings (37.38 ± 0.058 g) were allocated to five experimental treatments (TRTs) in 20 glass tanks for a feeding trial of about 70 days. KPH was supplemented to the diets by five levels: 0, 5, 10, 15, or 20%. A linear increase in the fish growth was observed by dietary KPH supplementation<i>.</i> The final body weight was increased by 6, 12, 15, and 18% for KPH5, KPH10, KPH15, and KPH20, respectively. The red blood cells, hemoglobin, and packed cell volume were increased in the KPH15 and KPH20 groups compared to the KPH0 group. The serum TP and β2-globulin levels were increased in KPH-supplemented TRTs (<i>P</i> &lt; 0.05). Reduced serum MDA levels and increased SOD activity were observed in KPH15 and KPH20 groups (<i>P</i> &lt; 0.01). The best intestinal histomorphometry was seen in the KPH15 group. Histological analysis of the treated groups’ liver and kidney sections revealed a comparatively normal histomorphology. Splenic tissues of the treated groups were normal, with more prevalent lymphoid elements, especially in the KPH10 and KPH15 groups. In conclusion, KPH could be a potential source of protein in Nile tilapia diets with good results for fish growth, blood biochemistry, and antioxidant status. KPH supplementation had no adverse effects on fish organs. The recommended inclusion level was 15% of the diet.\u0000</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"YOLOv10-UDFishNet: detection of diseased Takifugu rubripes juveniles in turbid underwater environments","authors":"Wan Tu,&nbsp;Hong Yu,&nbsp;Zijian Wu,&nbsp;Jian Li,&nbsp;Zhibo Cui,&nbsp;Zongyi Yang,&nbsp;Xin Zhang,&nbsp;Yue Wang","doi":"10.1007/s10499-024-01798-5","DOIUrl":"10.1007/s10499-024-01798-5","url":null,"abstract":"<div><p>Accurate detection of diseased <i>Takifugu rubripes</i> is essential for effective aquaculture disease management. However, turbid underwater conditions and the small scale of juvenile fish result in low precision. This paper proposes a diseased fish detection model called YOLOv10-UDFishNet, which comprehensively optimizes the backbone network, neck network, and detection head of YOLOv10. The model features an innovative backbone network with an auxiliary branch to capture multi-level image information, thereby enhancing feature extraction for diseased fish. Furthermore, space-to-depth convolution (SPD-Conv) replaces traditional strided convolution to preserve detail information, achieving accurate detection of diseased juveniles. In the neck network, we propose a feature fusion method based on high-level feature guidance (HFG), dynamically adjusting the concatenation of disparate feature maps to achieve higher recall. To sharpen the focus on diseased fish, the detection head utilizes the SlideLoss classification loss function, which improves detection performance. Ablation and comparison experiments are designed on a self-constructed dataset to verify the network’s superiority. The precision and recall of YOLOv10-UDFishNet were 94.0% and 90.1%, respectively, which are improved by 2.2% and 4.8%, respectively, over those of YOLOv10. Compared with current state-of-the-art underwater detection models such as DDEYOLOv9 and DFYOLO, our model achieves 2.5% and 3.1% relative improvements, respectively, in diseased fish recall. The results show that YOLOv10-UDFishNet is more adaptable to turbid underwater environments and exhibits enhanced effectiveness in diseased <i>Takifugu rubripes</i> detection.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field study on the dynamic characteristics of sewage collection in a circular aquaculture tank","authors":"Yanfei Wu,&nbsp;Fukun Gui,&nbsp;Jianxin Yang,&nbsp;Hongfang Qi,&nbsp;Hong Zhang,&nbsp;Yang Wang,&nbsp;Dejun Feng,&nbsp;Qingjing Zhang","doi":"10.1007/s10499-024-01755-2","DOIUrl":"10.1007/s10499-024-01755-2","url":null,"abstract":"<div><p>To address the low sewage collection performance in circular aquaculture tanks, this paper systematically investigates the influence of jet pipe flow rate <i>Q</i>, the deployment distance ratio <i>d</i>/<i>r</i> (where <i>d</i> represents the distance between the midpoint of the axis and the nearest tank wall, and <i>r</i> is the radius of the circular aquaculture tank), the deployment angle <i>θ</i>, and the structure of the jet pipe on the dynamic characteristics of sewage collection. In the field study, a high-definition camera was used to record the distribution of sewage, the distribution of the flow field in the aquaculture tank was measured by the Acoustic Doppler Velocimetry, and the distance between the sewage and the center of the tank was quantified. Furthermore, the sewage collection performance of the aquaculture tank was evaluated by comparing the maximum distance between the sewage and the center of the tank under different working conditions. The results indicated that the formation of an effective horizontal circulation in the circular aquaculture tank is the primary condition for sewage accumulation to the center of the tank, while different flow rate <i>Q</i> of the jet pipe, the deployment distance ratio <i>d</i>/<i>r</i>, the deployment angle <i>θ</i>, and the structure of the jet pipe all can affect the sewage collection effect in the tank. In the horizontal single-pipe single-hole jet pipe structure mode, the best sewage collection result was obtained at the deployment angle <i>θ</i> of 0°, the deployment distance ratio of 1/4, and the deployment flow rate <i>Q</i> of 7 <i>L</i>/s; when the deployment flow rate <i>Q</i> was less than 7 <i>L</i>/<i>s</i>, the sewage accumulated near the tank wall, and the sewage particle dispersion was relatively high. In the vertical single-pipe porous jet pipe structure mode, better sewage collection performance was achieved when the deployment flow rate <i>Q</i> was adjusted to 7 ~ 9 <i>L</i>/<i>s</i>, the deployment angle <i>θ</i> was set to 45°, and the deployment distance ratio <i>d</i>/<i>r</i> was set to 1/4. The sewage collection performance of the aquaculture tank under the vertical single-pipe porous jet pipe deployment mode was obviously better than that of the horizontal single-pipe single-hole jet pipe mode. These results can directly and effectively guide the inlet design of such aquaculture tanks to reduce the flow rate and energy consumption and improve sewage collection performance.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary BergaPur® supplementation enhanced the hematological, immune, biochemical, and antioxidative reactions of Nile tilapia, Oreochromis niloticus","authors":"Mohamed Ali,&nbsp;Hiam Elabd,&nbsp;Mohsen Azouz,&nbsp;Amany Abbass","doi":"10.1007/s10499-024-01750-7","DOIUrl":"10.1007/s10499-024-01750-7","url":null,"abstract":"<div><p>The existing trial investigated the effects of supplementing the diet of Nile tilapia with BergaPur^® on the hematology, immune response, biochemical factors, and antioxidative capacity. Four groups of fish with an average weight of 10.0 ± 0.5 g received BergaPur^®, de-oiled phospholipid complex at a rate of 0 (control), 1.0, 1.5, and 2.0 g/kg diet for 9 weeks. Sampling included blood, serum, and liver samples from the four groups at the end of each 3 weeks for a 9 weeks total experimental period. Hematologic readings, serum biochemical, and oxidative parameters were investigated. Findings included a significant (<i>P</i> &lt; 0.05) hematology augmentation (corpuscular Hb, packed cell volume, leukocytic, monocytic, and lymphocytic counts), and a remarked increased (<i>P</i> &lt; 0.05) serum proteins (albumin, globulin, and total) were recorded in BergaPur^® groups. Also, AST and ALT levels decreased significantly (<i>P</i> &lt; 0.05), while glucose levels increased significantly (<i>P</i> &lt; 0.05) in BergaPur^® groups at all sampling points. Immune (phagocytosis and lysozyme activity) and antioxidant (SOD, GPx, and CAT) parameters increased significantly (<i>P</i> &lt; 0.05) in BergaPur^® supplemented groups, compared to the control, while MDA level decreased obviously in the incorporated groups over the control. Our findings verified the positive effects of BergaPur^® on the hematological, immune, and biochemical parameters of <i>O Oreochromis niloticus</i>, with a suggestive concentration of 2 g/kg diet.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-024-01750-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of artichoke (Cynara scolymus) extract in Nile tilapia (Oreochromis niloticus) growth, and digestive enzymes, effect on oxidative and immune- and antioxidant-related gene expression before and after heat stress","authors":"Poulin Shohreh,&nbsp;Sedigheh Mohammadzadeh,&nbsp;Heba H. Mahboub,&nbsp;Pouya Ebrahimi,&nbsp;Hakimeh Gavzan,&nbsp;Mehdi Ahmadifar,&nbsp;Mohsen Shahriari Moghadam,&nbsp;Ehab EI-Haroun,&nbsp;Seyed Hossein Hoseinifar,&nbsp;Inês Guerreiro,&nbsp;Marina Paolucci,&nbsp;Ehsan Ahmadifar","doi":"10.1007/s10499-024-01794-9","DOIUrl":"10.1007/s10499-024-01794-9","url":null,"abstract":"<div><p>The current work studied the influence of artichoke (<i>Cynara scolymus</i>) extract (AE) on growth, digestive enzymes, mucus immune status, liver antioxidant capacity, and health in Nile tilapia (<i>Oreochromis niloticus</i>). For that, Nile tilapia was allocated into four groups and fed for 56 days on 0 (group T0), 100 (group T1), 150 (group T2), and 200 (group T3) mg of AE/kg feed. After the growth trial, fish were exposed to a heat stress challenge (over heat) and immune response, antioxidant enzymes, and stress factors which were measured pre and post-heat stress. Growth, feed efficiency, and protease, trypsin, and amylase activities significantly increased in fish fed the AE-supplemented diets. All measured mucus immune- (excepting alternative complement activity, ACH50) and liver antioxidant- and health-related enzymes and stress factors were negatively affected by the heat stress challenge. Dietary AE supplementation led to an increase in mucus total immunoglobulin, lysozyme, and ACH50 before and after heat stress. Independently of the heat stress, catalase and glutathione peroxidase (GPx) activities increased in fish fed diets T2 and T3, while malondialdehyde (MDA) content decreased. Liver alanine aminotransferase and aspartate aminotransferase (AST) activities, glucose, and cortisol content were decreased in fish fed AE-supplemented diets, both before and after heat stress. A significant interaction between diets and heat stress was observed only in mucus lysozyme, liver MDA, GPx, and AST. Prior to being subjected to heat stress, the expression of <i>IL-1b</i>, <i>lysozyme</i>, <i>GPx</i>, and <i>CAT</i> exhibited significant upregulation compared to the fish fed control diet (<i>p</i> &lt; 0.05). In conclusion, the use of AE in tilapia diets has the ability to boost growth performance, digestive and antioxidant enzymes activity, immune status, hepatic function, and mitigate stress in tissue prior and post-exposure to heat stress, and it can add to fish diet to achieve aquaculture sustainability and fish well-being.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of dietary supplementation with Enteromorpha prolifera polysaccharide on the growth, immunity, and antioxidant capacity of spotted sea bass Lateolabrax maculatus","authors":"Zhongying Long,&nbsp;Longhui Liu,&nbsp;Huihui Qin,&nbsp;Sishun Zhou,&nbsp;Lumin Kong,&nbsp;Jianrong Ma,&nbsp;Hao Lin,&nbsp;Zhangfan Huang,&nbsp;Yi Lin,&nbsp;Zhongbao Li","doi":"10.1007/s10499-024-01792-x","DOIUrl":"10.1007/s10499-024-01792-x","url":null,"abstract":"<div><p>The aim of this study was to investigate the effects of dietary supplementation with <i>Enteromorpha prolifera</i> polysaccharide (EPP) on the growth, antioxidant, and immune capacity of spotted sea bass <i>Lateolabrax maculatus</i>. EPP were added to the diet of spotted sea bass in gradients (0, 3, 6, 9, 12, and 15 g/kg). A total of 540 similarly sized and healthy spotted sea bass were randomly divided into 6 groups and fed the corresponding diet for 49 days. The results showed that compared with not adding an EPP group, the WGR at 9 g/kg EPP supplemental level was significantly increased (<i>P</i> &lt; 0.05); the SGR at 12 g/kg EPP supplemental level was significantly increased (<i>P</i> &lt; 0.05). In addition, the supplementation of EPP could improve the immunity of spotted sea bass to a certain extent, but excessive supplementation may cause immunosuppression. The addition of 6 and 9 g/kg EPP significantly increased the activities of IgM, C3, and ACP in serum. After the increase in supplemental levels, the relevant immune indexes were observed to be significantly decreased. On the other hand, dietary supplementation with EPP showed disorganized results in terms of antioxidant capacity. Transcriptome analysis showed that in the liver, major differential genes were enriched in apoptosis-related pathways, and the related HSP90 gene was downregulated. In the head kidney, differential genes were mainly enriched in immune-related pathways, and the related FTH1 and LST8 genes were observed to be upregulated. Therefore, EPP may regulate the immune capacity of spotted sea bass by regulating immune-related pathways and apoptosis-related pathways.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and application of an evaluation index system for high-quality development in aquaculture","authors":"Shanshan Zhu,&nbsp;Chao Lyu","doi":"10.1007/s10499-024-01784-x","DOIUrl":"10.1007/s10499-024-01784-x","url":null,"abstract":"<div><p>This study aims to develop an evaluation indicator system for the high-quality development of aquaculture in China, guided by relevant national policies. It uses the entropy weight method (EWM) and the CRITIC method to conduct an empirical assessment of major fishing provinces, providing a reference for the formulation of science-based industry policies. The research constructs a comprehensive evaluation framework based on five key dimensions: industrial scale and efficiency, green development, technology and infrastructure, industrial integration and transformation, and industry management and services. Taking local conditions into account, the study employs diversified evaluation methods for an in-depth analysis. The results reveal significant performance differences across provinces in these dimensions. Shandong leads in industrial integration and transformation, Guangdong excels in green development, and Jiangsu stands out in technological innovation and industry management. The evaluation system developed in this study provides a scientific basis for promoting the high-quality development of aquaculture and is of great significance for driving industrial transformation and upgrading, as well as supporting the sustainable development of fisheries globally.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10499-024-01784-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective and haemato-immunological effect of seaweed crude extracts against Tilapia Lake Virus (TiLV) in Nile tilapia, Oreochromis niloticus","authors":"Kaviyarasu Periyannan,&nbsp;Sivasankar Panchavarnam,&nbsp;Chrisolite Bagthasingh,&nbsp;Subash Palaniappan,&nbsp;Priyadharshini Michael,&nbsp;Mageshkumar Palraj,&nbsp;Mohamed Mansoor Mohaideenpitchai,&nbsp;Selvamagheswaran Muthumariyapan","doi":"10.1007/s10499-024-01795-8","DOIUrl":"10.1007/s10499-024-01795-8","url":null,"abstract":"<div><p>Stimulation of non-specific immune responses in Nile tilapia by seaweed-based natural compounds is a potential solution for the deterrence of Tilapia Lake Virus (TiLV) outbreaks. The present study investigated the protective and immunostimulatory effects of different seaweed extracts against TiLV. The study deals with hot water extracts of ten seaweeds in total namely <i>Gracilaria edulis, Ulva fasciata, Caulerpa toxifolia, Turbinaria ornata, Enteromorpha compressa, Sargassum wightii, Sargassum ilicifolium, Sargassum muticum, Hynea musciformis</i> and <i>Chaetomorpha aerea</i> collected from the Gulf of Mannar coast of Mandapam and Rameswaram, Tamil Nadu. Among these, extracts of three seaweeds such as <i>S. ilicifolium, U. fasciata,</i> and <i>E. compressa</i> exhibited notable antiviral effects by inhibiting cytopathic effect without cell toxicity in striped snakehead (SSN1) cell line. These seaweed extracts were further incorporated into artificial pellet feed at different concentrations (100, 300 and 500 mg/kg). Of which, tilapia fed with diet containing <i>S. ilicifolium</i> at 500 mg/kg of feed showed the highest RPS value (58.34%) followed by <i>U. fasciata</i> (50%) and <i>E. compressa</i> (41.67%). Additionally, significant (<i>P</i> &lt; 0.05) improvements in haematological parameters, immunological parameters and immune gene expression were observed in the <i>S. ilicifolium</i>-fed group at 24, 48 and 72 h post-infection. The findings highlight <i>S. ilicifolium</i> extract as a natural dietary immunostimulant that enhances the health, productivity and management of TiLV in tilapia farming, promoting a sustainable approach to mitigate disease outbreaks in commercial aquaculture.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gynogenesis in the spotted catfish Pimelodus maculatus using heterologous gametes from the yellowtail-tetra (Astyanax altiparanae): a key point to achieve monosex female populations in Neotropical catfishes","authors":"Rafaela Manchin Bertolini,&nbsp;Lucia Suárez López,&nbsp;Bruna dos Santos Machado,&nbsp;Nivaldo Ferreira do Nascimento,&nbsp;José Augusto Senhorini,&nbsp;Norberto Castro Vianna,&nbsp;George Shigueki Yasui","doi":"10.1007/s10499-024-01785-w","DOIUrl":"10.1007/s10499-024-01785-w","url":null,"abstract":"<div><p>Monosex populations in aquaculture is important to increase performance for subsequent food supply. Gynogenesis is the main tool to achieve monosex population within only one generation, since it provides only maternal inheritance. The present study aimed to achieve gynogenetic individuals of spotted catfish (<i>Pimelodus maculatus</i>), an important Neotropical species in which the female presents better growth performance. Sperm of yellowtail-tetra (<i>Astyanax altiparanae</i>) was UV-irradiated (80 mJ/cm²) in order to inactivate paternal genome. Afterwards, oocytes of <i>P. maculatus</i> were fertilized with the irradiated sperm and heat-shocked (38 °C for 2 min, 2 mpf) for diploidization by prevention of the release of the 2nd polar body. Diploid progenies derived from <i>P. maculatus</i> couples served as controls, and a non-treated haploid was also produced. Flow cytometric analysis and chromosome number obtained by cytogenetic analysis confirmed that gynogenetic individuals had the same number of chromosomes as the control group, indicating that sperm inactivation and diploidization was successful. Genotyping by microsatellite markers confirmed the maternal inheritance in these gynogenetics. Macroscopic visualization of the gonads revealed an increased number of females (<i>n</i> = 48) and only two males, suggesting a homogametic sex determination system (XX/XY). Finally, an efficient protocol for inducing meiotic gynogenesis in <i>P. maculatus</i> was established using irradiated sperm from <i>A. altiparanae</i>. This protocol can apply to produce monosex population and clonal lines in the <i>P. maculatus</i> species and can contribute for future studies in basic and applied sciences.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}