Effects of Vibrio harveyi and oligochitosan non-specific immune response of hybrid groupers (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) supplementation

IF 2.2 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-04-24 DOI:10.1007/s10499-025-01972-3

Lixin Ma, Zhilong Chen, Hao Zhao, Xiaomin Zheng, Yutao Li, Zhendong Qin, Li Lin, Fei Shi

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引用次数: 0

Abstract

Vibrio harveyi is a major pathogen in aquaculture, severely affecting hybrid groupers. This study investigates the effects of V. harveyi infection and oligochitosan treatment on the growth, biochemical parameters, and immune responses of hybrid groupers. The results show that V. harveyi significantly reduced body weight (P < 0.05), body length (P < 0.05), and head kidney weight (P < 0.05). Histopathological analysis revealed substantial damage to head kidney tissue and a significant decrease in head kidney cell count (P < 0.05). Biochemical analysis of serum and head kidney samples showed that V. harveyi increased glutamic-pyruvic transaminase (GPT) activity (P < 0.05) and reduced catalase (CAT) activity (P < 0.01), while not affecting glutamic-oxaloacetic transaminase (GOT) or superoxide dismutase (SOD) activities. V. harveyi also increased GPT and GOT activities (P < 0.05), and oligochitosan reduced GPT (P < 0.01) and SOD activities (P < 0.05) in the head kidney. Quantitative real-time PCR results indicated that V. harveyi downregulated interleukin-10 (IL- 10) (P < 0.05) and upregulated interleukin-1β (IL- 1β) (P < 0.05), interleukin-8 (IL- 8) (P < 0.05), nuclear factor kappa-light-chain-enhancer of activated B cells p65 (p65) (P < 0.05), and toll-like receptor 3 (TLR- 3) (P < 0.05), which were partially reversed by oligochitosan. V. harveyi also upregulated cysteine-dependent aspartate-specific protease-3 (caspase- 3) (P < 0.05) and bcl-2-associated x protein (BAX) (P < 0.05) expression, increasing apoptosis, which was alleviated by oligochitosan supplementation, as shown by TdT-mediated dUTP nick-end labeling (TUNEL) assay (P < 0.01). In conclusion, V. harveyi induces inflammation and impairs immune response in hybrid groupers, while oligochitosan can mitigate these effects and enhance immunity.

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杂交石斑鱼（斑石斑鱼♀× lanceolatus Epinephelus♀）添加哈威弧菌和低聚壳聚糖非特异性免疫反应的影响

哈威弧菌是水产养殖中的主要病原菌，严重影响杂交石斑鱼。本研究探讨了harveyi v感染和低聚壳聚糖处理对杂交石斑鱼生长、生化指标和免疫应答的影响。结果表明：哈威菌显著降低了体重（P < 0.05）、体长（P < 0.05）和头肾重（P < 0.05）。组织病理学分析显示头部肾组织严重损伤，头部肾细胞计数显著减少（P < 0.05）。血清和头肾样品的生化分析表明，harvey菌提高了谷丙转氨酶（GPT）活性（P < 0.05），降低了过氧化氢酶（CAT）活性（P < 0.01），而对谷草转氨酶（GOT）和超氧化物歧化酶（SOD）活性没有影响。山楂多糖还能提高头肾GPT和GOT活性（P < 0.05），低聚壳聚糖降低头肾GPT （P < 0.01）和SOD活性（P < 0.05）。实时荧光定量PCR结果显示，V. harveyi下调了活化B细胞的白细胞介素-10 (IL- 10) (P < 0.05)，上调了活化B细胞的白细胞介素-1β (IL- 1β) （P < 0.05）、白细胞介素-8 (IL- 8) （P < 0.05）、核因子kappa轻链增强子p65 (p65) （P < 0.05）和toll样受体3 (TLR- 3) (P < 0.05)，这些上调被低聚壳聚糖部分逆转。V. harveyi还上调半胱氨酸依赖的天冬氨酸特异性蛋白酶-3 (caspase- 3) （P < 0.05）和bcl-2相关x蛋白（BAX）（P < 0.05）的表达，增加细胞凋亡，tdt介导的dUTP镍端标记（TUNEL）实验显示，补充寡糖可以减轻细胞凋亡（P < 0.01）。综上所述，杂石斑鱼诱导炎症反应，损害免疫反应，而低壳聚糖可以减轻这些影响，增强免疫。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.